{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T18:49:31Z","timestamp":1783104571978,"version":"3.54.6"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the basic research and frontier exploration projects of Chongqing","award":["cstc2018jcyjAX0075"],"award-info":[{"award-number":["cstc2018jcyjAX0075"]}]},{"name":"the basic research and frontier exploration projects of Chongqing","award":["20210607223136209"],"award-info":[{"award-number":["20210607223136209"]}]},{"name":"the basic research and frontier exploration projects of Chongqing","award":["2022 (9)"],"award-info":[{"award-number":["2022 (9)"]}]},{"name":"the subsidy fund for the development of national silk in Chongqing","award":["cstc2018jcyjAX0075"],"award-info":[{"award-number":["cstc2018jcyjAX0075"]}]},{"name":"the subsidy fund for the development of national silk in Chongqing","award":["20210607223136209"],"award-info":[{"award-number":["20210607223136209"]}]},{"name":"the subsidy fund for the development of national silk in Chongqing","award":["2022 (9)"],"award-info":[{"award-number":["2022 (9)"]}]},{"name":"the Chongqing modern mountain characteristic high-efficiency agriculture sericulture industry technical system post-scientist","award":["cstc2018jcyjAX0075"],"award-info":[{"award-number":["cstc2018jcyjAX0075"]}]},{"name":"the Chongqing modern mountain characteristic high-efficiency agriculture sericulture industry technical system post-scientist","award":["20210607223136209"],"award-info":[{"award-number":["20210607223136209"]}]},{"name":"the Chongqing modern mountain characteristic high-efficiency agriculture sericulture industry technical system post-scientist","award":["2022 (9)"],"award-info":[{"award-number":["2022 (9)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Transmembrane emp24 domain (TMED) proteins have been extensively studied in mammalian embryonic development, immune regulation, and signal transduction. However, their role in insects, apart from Drosophila melanogaster, remains largely unexplored. Our previous study demonstrated the abundant expression of BmTMED6 across all stages and tissues of the silkworm. In this study, we investigate the function of BmTMED6 in reproduction. We observe significant differences in the expression of BmTMED6 between male and female silkworms, particularly in the head and fatboby, during the larval stage. Furthermore, qRT-PCR and WB analysis reveal substantial variation in BmTMED6 levels in the ovaries during pupal development, suggesting a potential association with silkworm female reproduction. We find that reducing TMED6 expression significantly decreases the number of eggs laid by female moths, leading to an accumulation of unlaid eggs in the abdomen. Moreover, downregulation of BmTMED6 leads to a decrease in the expression of BmDop2R1 and BmDop2R2, while overexpression of BmTMED6 in vitro has the opposite effect. These indicate that BmTMED6 plays a role in oviposition in female moths, potentially through the dopamine signaling pathway. This study provides a new regulatory mechanism for female reproduction in insects.<\/jats:p>","DOI":"10.3390\/insects15020103","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T09:24:08Z","timestamp":1706865848000},"page":"103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Role of BmTMED6 in Female Reproduction in Silkworm, Bombyx mori"],"prefix":"10.3390","volume":"15","author":[{"given":"Chunyang","family":"Wang","sequence":"first","affiliation":[{"name":"College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunmei","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfu","family":"Xiao","sequence":"additional","affiliation":[{"name":"Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anlian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Sericultural Research Institute, Sichuan Academy of Agricultural Sciences, Nanchong 637000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China"},{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1515\/bmc-2012-0028","article-title":"Diverse roles for the p24 family of proteins in eukaryotic cells","volume":"3","author":"Schuiki","year":"2012","journal-title":"Biomol. 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