{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:22:27Z","timestamp":1780568547727,"version":"3.54.1"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202296"],"award-info":[{"award-number":["32202296"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CAAS-ASTIP-TRICAAS"],"award-info":[{"award-number":["CAAS-ASTIP-TRICAAS"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015335","name":"Modern Agricultural Industry Technology System","doi-asserted-by":"publisher","award":["32202296"],"award-info":[{"award-number":["32202296"]}],"id":[{"id":"10.13039\/501100015335","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015335","name":"Modern Agricultural Industry Technology System","doi-asserted-by":"publisher","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}],"id":[{"id":"10.13039\/501100015335","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015335","name":"Modern Agricultural Industry Technology System","doi-asserted-by":"publisher","award":["CAAS-ASTIP-TRICAAS"],"award-info":[{"award-number":["CAAS-ASTIP-TRICAAS"]}],"id":[{"id":"10.13039\/501100015335","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovative Program of the Chinese Academy of Agricultural Sciences","award":["32202296"],"award-info":[{"award-number":["32202296"]}]},{"name":"Innovative Program of the Chinese Academy of Agricultural Sciences","award":["CARS-19"],"award-info":[{"award-number":["CARS-19"]}]},{"name":"Innovative Program of the Chinese Academy of Agricultural Sciences","award":["CAAS-ASTIP-TRICAAS"],"award-info":[{"award-number":["CAAS-ASTIP-TRICAAS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The tea black tussock moth (Dasychira baibarana), a devastating pest in Chinese tea plantations, uses a ternary Type-II pheromone blend containing (3Z,6Z)-cis-9,10-epoxyhenicosa-3,6-diene (Z3,Z6,epo9-21:H), (3Z,6Z,11E)-cis-9,10-epoxyhenicosa-3,6,11-triene (Z3,Z6,epo9,E11-21:H), and (3Z,6Z)-henicosa-3,6-dien-11-one (Z3,Z6-21:11-one) for mate communication. To elucidate the P450 candidates associated with the biosynthesis of these sex pheromone components, we sequenced the female D. baibarana pheromone gland and the abdomen excluding the pheromone gland. A total of 75 DbP450s were identified. Function annotation suggested six CYPs were orthologous genes that are linked to molting hormone metabolism, and eight antennae specifically and significantly up-regulated CYPs may play roles in odorant processing. Based on a combination of comparative RNAseq, phylogenetic, and tissue expression pattern analysis, one CYP4G with abdomen specifically predominant expression pattern was likely to be the P450 decarbonylase, while the pheromone-gland specifically and most abundant CYP341B65 was the most promising epoxidase candidate for the D. baibarana sex pheromone biosynthesis. Collectively, our research laid a valuable basis not only for further functional elucidation of the candidate P450 decarbonylase and epoxidase for the sex pheromone biosynthesis but also for understanding the physiological functions and functional diversity of the CYP gene superfamily in the D. baibarana.<\/jats:p>","DOI":"10.3390\/insects15020139","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T10:40:15Z","timestamp":1708339215000},"page":"139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Transcriptome-Wide Identification of Cytochrome P450s in Tea Black Tussock Moth (Dasychira baibarana) and Candidate Genes Involved in Type-II Sex Pheromone Biosynthesis"],"prefix":"10.3390","volume":"15","author":[{"given":"Tiekuang","family":"Wang","sequence":"first","affiliation":[{"name":"Qinghai Academy of Agriculture and Forestry Science, Qinghai University, Xining 810016, China"},{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xufei","family":"Liu","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongxiu","family":"Luo","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Cai","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoqun","family":"Li","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Bian","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6001-9139","authenticated-orcid":false,"given":"Chunli","family":"Xiu","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongmao","family":"Chen","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiurong","family":"Li","sequence":"additional","affiliation":[{"name":"Qinghai Academy of Agriculture and Forestry Science, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6151-9808","authenticated-orcid":false,"given":"Nanxia","family":"Fu","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2452","DOI":"10.1021\/jf073161w","article-title":"Sex pheromone of browntail moth, Euproctis chrysorrhea (L.): Synthesis and field deployment","volume":"56","author":"Khrimian","year":"2008","journal-title":"J. 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