{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:13:20Z","timestamp":1785208400907,"version":"3.55.0"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:00:00Z","timestamp":1712102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014271","name":"University of Life Sciences in Lublin","doi-asserted-by":"publisher","award":["LKE\/S\/64\/2021-2023"],"award-info":[{"award-number":["LKE\/S\/64\/2021-2023"]}],"id":[{"id":"10.13039\/501100014271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The morphological changes in fat body cells, tergal gland cells, and the surface areas of the cell nuclei were determined in queen bees of the subspecies Apis mellifera carnica. This study focused on 1-, 8-, and 20-day-old uninseminated females kept in colonies, analyzing cells from three locations in the abdomen: the sternite, and tergites III and V. The oenocytes in the sternites were large, oval\/circular with a centrally located nucleus, while in tergites III and V, they were small and triangular in the 1-day-old queens. During the first week of life, these cells in tergites III and V change their shape to oval and increase their sizes. The initially light yellow and then dark yellow granularities in the oenocytes of the fat body appear along with the advancing age of the queens. The trophocytes (sternites, tergites III and V) in the 1-day-old queens were completely filled with droplets of different sizes. In the 8- and 20-day-old queens, the number and size of the droplets decreased in the trophocytes of tergites III and V. The tergal gland cells had a centrally located cell nucleus in the 1-, 8- and 20-day-old queens. The dark granularities in these cells were visible only in the 20-day-old queens. Different morphological images of the fat body at the sternite, and tergites III and V, and the difference in the size of the oenocyte cell nuclei may indicate various functions of the fat body depending on its location. Characterization of the changes in the morphology of the fat body, taking into account its segmental character, and the tergal glands requires further research in older queens, e.g., one-year-old, brooding queens.<\/jats:p>","DOI":"10.3390\/insects15040244","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T00:58:38Z","timestamp":1712105918000},"page":"244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Morphological Image of Fat Body and Tergal Gland Cells in Uninseminated Apis mellifera Queen Bees"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9504-2216","authenticated-orcid":false,"given":"Milena","family":"Jaremek","sequence":"first","affiliation":[{"name":"Department of Invertebrate Ecophysiology and Experimental Biology, Faculty of Environmental Biology, University of Life Sciences in Lublin, Do\u015bwiadczalna 50a, 20-280 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4968-4621","authenticated-orcid":false,"given":"Krzysztof","family":"Olszewski","sequence":"additional","affiliation":[{"name":"Subdepartment of Apidology, Institute of Biological Basis of Animal Production, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Chobotow","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 20-400 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1313-9880","authenticated-orcid":false,"given":"Aneta","family":"Strachecka","sequence":"additional","affiliation":[{"name":"Department of Invertebrate Ecophysiology and Experimental Biology, Faculty of Environmental Biology, University of Life Sciences in Lublin, Do\u015bwiadczalna 50a, 20-280 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tautz, J. (2008). The Buzz about Bees, Springer Science and Business Media LLC.","DOI":"10.1007\/978-3-540-78729-7"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Bry\u015b, M.S., Skowronek, P., and Strachecka, A. (2021). Pollen Diet\u2014Properties and Impact on a Bee Colony. Insects, 12.","DOI":"10.3390\/insects12090798"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.cvfa.2021.06.006","article-title":"Honey Bee Nutrition","volume":"37","author":"Tsuruda","year":"2021","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"102511","DOI":"10.1016\/j.jksus.2022.102511","article-title":"Impact of Different Pollen Protein Diets on the Physiology of Apis mellifera L. (Hymenoptera: Apidae) Workers from Essential Plant Sources","volume":"35","author":"Idrees","year":"2023","journal-title":"J. King Saud Univ.-Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Paleolog, J., Wilde, J., Gancarz, M., Wi\u0105cek, D., Nawrocka, A., and Strachecka, A. (2023). Imidacloprid Pesticide Causes Unexpectedly Severe Bioelement Deficiencies and Imbalance in Honey Bees Even at Sublethal Doses. Animals, 13.","DOI":"10.3390\/ani13040615"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"14790","DOI":"10.1073\/pnas.0906970106","article-title":"Changes in Transcript Abundance Relating to Colony Collapse Disorder in Honey Bees (Apis mellifera)","volume":"106","author":"Johnson","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"18466","DOI":"10.1073\/pnas.1314923110","article-title":"Neonicotinoid Clothianidin Adversely Affects Insect Immunity and Promotes Replication of a Viral Pathogen in Honey Bees","volume":"110","author":"Cavaliere","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Strachecka, A., Kuszewska, K., Olszewski, K., Skowronek, P., Grzybek, M., Grabowski, M., Paleolog, J., and Woyciechowski, M. (2022). Activities of Antioxidant and Proteolytic Systems and Biomarkers in the Fat Body and Hemolymph of Young Apis mellifera Females. Animals, 12.","DOI":"10.3390\/ani12091121"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Strachecka, A., Migda\u0142, P., Kuszewska, K., Skowronek, P., Grabowski, M., Paleolog, J., and Woyciechowski, M. (2021). Humoral and Cellular Defense Mechanisms in Rebel Workers of Apis mellifera. Biology, 10.","DOI":"10.3390\/biology10111146"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1016\/j.jinsphys.2008.07.016","article-title":"The Ontogeny of Immunity: Development of Innate Immune Strength in the Honey Bee (Apis mellifera)","volume":"54","author":"Dres","year":"2008","journal-title":"J. Insect Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1111\/j.1365-2583.2006.00682.x","article-title":"Immune Pathways and Defence Mechanisms in Honey Bees Apis mellifera","volume":"15","author":"Evans","year":"2006","journal-title":"Insect Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1111\/j.1365-2583.2006.00684.x","article-title":"Comparative Analysis of Serine Protease-Related Genes in the Honey Bee Genome: Possible Involvement in Embryonic Development and Innate Immunity","volume":"15","author":"Zou","year":"2006","journal-title":"Insect Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Skowronek, P., W\u00f3jcik, \u0141., and Strachecka, A. (2021). Fat Body\u2014Multifunctional Insect Tissue. Insects, 12.","DOI":"10.3390\/insects12060547"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Strachecka, A., Chobotow, J., Kuszewska, K., Olszewski, K., Skowronek, P., Bry\u015b, M., Paleolog, J., and Woyciechowski, M. (2022). Morphology of Nasonov and Tergal Glands in Apis mellifera Rebels. Insects, 13.","DOI":"10.3390\/insects13050401"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13887","DOI":"10.1038\/s41598-021-93357-8","article-title":"Segmentation of the Subcuticular Fat Body in Apis mellifera Females with Different Reproductive Potentials","volume":"11","author":"Strachecka","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1093\/jee\/97.3.752","article-title":"Bacterial Probiotics Induce an Immune Response in the Honey Bee (Hymenoptera: Apidae)","volume":"97","author":"Evans","year":"2004","journal-title":"J. Econ. Entomol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1673\/1536-2442(2006)6[1:ATAOTA]2.0.CO;2","article-title":"A Transcriptome Analysis of the Aedes aegypti Vitellogenic Fat Body","volume":"6","author":"Feitosa","year":"2006","journal-title":"J. Insect Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1590\/S0074-02762010000300003","article-title":"Culex Quinquefasciatus Vitellogenesis: Morphological and Biochemical Aspects","volume":"105","author":"Cardoso","year":"2010","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1365-2583.2006.00647.x","article-title":"Anopheles Gambiae P450 Reductase Is Highly Expressed in Oenocytes and in Vivo Knockdown Increases Permethrin Susceptibility","volume":"15","author":"Lycett","year":"2006","journal-title":"Insect Mol. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1590\/S0074-02762011000300009","article-title":"Insights into the Transcriptome of Oenocytes from Aedes aegypti Pupae","volume":"106","author":"Martins","year":"2011","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1146\/annurev.ento.48.091801.112657","article-title":"Male Accessory Gland Secretions: Modulators of Female Reproductive Physiology and Behavior","volume":"48","author":"Gillott","year":"2003","journal-title":"Annu. Rev. Entomol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev-ento-112408-085356","article-title":"Insect Fat Body: Energy, Metabolism, and Regulation","volume":"55","author":"Arrese","year":"2009","journal-title":"Annu. Rev. Entomol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1051\/apido:19990407","article-title":"Honeybee Queen Tergal Gland Secretion Affects Ovarian Development in Caged Workers","volume":"30","author":"Wossler","year":"1999","journal-title":"Apidologie"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1023\/A:1020839505622","article-title":"The Releaser Effects of the Tergal Gland Secretion of Queen Honeybees (Apis mellifera)","volume":"12","author":"Wossler","year":"1999","journal-title":"J. Insect Behav."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s13592-018-0628-5","article-title":"Tergal Gland Components of Reproductively Dominant Honey Bee Workers Have Both Primer and Releaser Effects on Subordinate Workers","volume":"50","author":"Okosun","year":"2019","journal-title":"Apidologie"},{"key":"ref_26","first-page":"1","article-title":"Standard Methods for Rearing and Selection of Apis mellifera Queens","volume":"52","author":"Andonov","year":"2013","journal-title":"J. Apic. Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Strachecka, A., Chobotow, J., Paleolog, J., \u0141os, A., Schulz, M., Teper, D., Kucharczyk, H., and Grzybek, M. (2017). Insights into the Biochemical Defence and Methylation of the Solitary Bee Osmia Rufa L: A Foundation for Examining Eusociality Development. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0176539"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1590\/S0073-47212004000400010","article-title":"Carminda Da Cruz-Landim, & Protein Content and Electrophoretic Profile of Fat Body and Ovary Extracts from Workers of Melipona Quadrifasciata Anthidioides (Hymenoptera, Meliponini)","volume":"94","year":"2004","journal-title":"Iheringia. S\u00e9rie Zool."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1365-2435.2008.01419.x","article-title":"Ageing in a Eusocial Insect: Molecular and Physiological Characteristics of Life Span Plasticity in the Honey Bee","volume":"22","author":"Amdam","year":"2008","journal-title":"Funct. Ecol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111562","DOI":"10.1016\/j.mad.2021.111562","article-title":"The Adult Lifespan of the Female Honey Bee (Apis mellifera): Metabolic Rate, AGE Pigment and the Effect of Dietary Fatty Acids","volume":"199","author":"Martin","year":"2021","journal-title":"Mech. Ageing Dev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6852","DOI":"10.1038\/s41598-021-86078-5","article-title":"Novel Structure in the Nuclei of Honey Bee Brain Neurons Revealed by Immunostaining","volume":"11","author":"Hurd","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1002\/cne.900270105","article-title":"Nuclear Size in the Nerve Cells of the Bee during the Life Cycle","volume":"27","author":"Smallwood","year":"1916","journal-title":"J. Comp. Neurol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1034\/j.1600-0749.2002.00056.x","article-title":"Comparative Biochemistry of Eumelanogenesis and the Protective Roles of Phenoloxidase and Melanin in Insects","volume":"15","author":"Sugumaran","year":"2002","journal-title":"Pigment Cell Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S1534-5807(02)00267-8","article-title":"An Immune-Responsive Serpin Regulates the Melanization Cascade in Drosophila","volume":"3","author":"Han","year":"2002","journal-title":"Dev. Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0968-4328(00)00071-8","article-title":"Ferritin in Iron Containing Granules from the Fat Body of the Honeybees Apis mellifera and Scaptotrigona postica","volume":"33","author":"Keim","year":"2002","journal-title":"Micron"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4393","DOI":"10.1242\/jeb.00709","article-title":"Chitin Metabolism in Insects: Structure, Function and Regulation of Chitin Synthases and Chitinases","volume":"206","author":"Merzendorfer","year":"2003","journal-title":"J. Exp. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1016\/j.ibmb.2004.08.005","article-title":"Phenoloxidase Activity in Apis mellifera Honey Bee Pupae, and Ecdysteroid-Dependent Expression of the Prophenoloxidase MRNA","volume":"34","author":"Zufelato","year":"2004","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.ibmb.2006.01.012","article-title":"Model Reactions for Insect Cuticle Sclerotization: Cross-Linking of Recombinant Cuticular Proteins upon Their Laccase-Catalyzed Oxidative Conjugation with Catechols","volume":"36","author":"Suderman","year":"2006","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0022-1910(85)90002-2","article-title":"Pheromonal Activity and Fine Structure of the Mandibular Glands of Honeybee Drones (Apis mellifera L.) (Insecta, Hymenoptera, Apidae)","volume":"31","author":"Lensky","year":"1985","journal-title":"J. Insect Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1080\/00218839.1964.11100085","article-title":"Pheromones of the Honeybee: Biological Studies of the Mandibular Gland Secretion of the Queen","volume":"3","author":"Butler","year":"1964","journal-title":"J. Apic. Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Brutscher, L.M., Baer, B., and Ni\u00f1o, E.L. (2019). Putative Drone Copulation Factors Regulating Honey Bee (Apis mellifera) Queen Reproduction and Health: A Review. Insects, 10.","DOI":"10.3390\/insects10010008"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10886-017-0912-2","article-title":"Evaluating the Role of Drone-Produced Chemical Signals in Mediating Social Interactions in Honey Bees (Apis mellifera)","volume":"44","author":"Villar","year":"2018","journal-title":"J. Chem. Ecol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6293","DOI":"10.1038\/s41598-017-06241-9","article-title":"Virgin Queen Attraction toward Males in Honey Bees","volume":"7","author":"Bastin","year":"2017","journal-title":"Sci. Rep."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/15\/4\/244\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:22:44Z","timestamp":1760106164000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/15\/4\/244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,3]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["insects15040244"],"URL":"https:\/\/doi.org\/10.3390\/insects15040244","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,3]]}}}