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This blend contains (Z,Z,Z)-3,6,9-octadecatriene, (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene, and (Z,Z)-3,9-cis-6,7-epoxy-nonadecadiene. To elucidate the genes related to the biosynthesis of these sex pheromone components, transcriptome sequencing of the female E. obliqua pheromone gland and the abdomen without pheromone gland was performed. Comparative RNAseq analyses identified 52 putative genes, including 7 fatty acyl-CoA elongases (ELOs), 9 fatty acyl-CoA reductases (FARs), 1 decarbonylase (DEC), 3 lipophorins (LIPs), and 32 cytochrome P450 enzymes (CYPs). Tissue expression profiles revealed that two ELOs (ELO3 and ELO5), two FARs (FAR2 and FAR9), one DEC (CYP4G173), and one LIP (LIP1) displayed either abdomen-centric or -specific expression, suggesting potential roles in sex pheromone biosynthesis within the oenocytes of E. obliqua. Furthermore, the tissue expression patterns, combined with phylogenetic analysis, showed that CYP340BD1, which was expressed specifically and predominantly only in the pheromone gland, was clustered with the previously reported epoxidases, highlighting its potential role in the epoxidation of the unsaturated polytriene sex pheromone components. Collectively, our research provides valuable insights into the genes linked to sex pheromone biosynthesis.<\/jats:p>","DOI":"10.3390\/insects15040276","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T10:24:26Z","timestamp":1713176666000},"page":"276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of Candidate Genes Associated with Type-II Sex Pheromone Biosynthesis in the Tea Geometrid (Ectropis obliqua) (Lepidoptera: Geometridae)"],"prefix":"10.3390","volume":"15","author":[{"given":"Changxia","family":"Xu","sequence":"first","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"},{"name":"College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6151-9808","authenticated-orcid":false,"given":"Nanxia","family":"Fu","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoming","family":"Cai","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoqun","family":"Li","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Bian","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6001-9139","authenticated-orcid":false,"given":"Chunli","family":"Xiu","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongmao","family":"Chen","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0098-6458","authenticated-orcid":false,"given":"Zongxiu","family":"Luo","sequence":"additional","affiliation":[{"name":"Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/b95450","article-title":"Insect pheromone biosynthesis","volume":"Volume 239","author":"Schulz","year":"2004","journal-title":"Chemistry of Pheromones and Other Semiochemicals I"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.yhbeh.2023.105330","article-title":"Methoprene-tolerant and Kruppel homolog 1 are actors of juvenile hormone-signaling controlling the development of male sexual behavior in the moth Agrotis ipsilon","volume":"150","author":"Aguilar","year":"2023","journal-title":"Horm. Behav."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1584\/jpestics.D20-046","article-title":"Semiochemicals containing lepidopteran sex pheromones: Wonderland for a natural product chemist","volume":"45","author":"Ando","year":"2020","journal-title":"J. Pestic. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.copbio.2020.07.011","article-title":"Insect sex pheromone production in yeasts and plants","volume":"65","author":"Petkevicius","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1002\/arch.940300104","article-title":"Hormonal-regulation of sex-pheromone biosynthesis in the turnip moth, Agrotis segetum","volume":"30","author":"Zhu","year":"1995","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1007\/s10886-013-0292-1","article-title":"Biosynthesis and PBAN-regulated transport of pheromone polyenes in the winter moth, Operophtera brumata","volume":"39","author":"Wang","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.ibmb.2011.02.004","article-title":"Biosynthetic pathways of the sex pheromone components and substrate selectivity of the oxidation enzymes working in pheromone glands of the fall webworm, Hyphantria cunea","volume":"41","author":"Kiyota","year":"2011","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.ibmb.2011.05.003","article-title":"Terminal fatty-acyl-CoA desaturase involved in sex pheromone biosynthesis in the winter moth (Operophtera brumata)","volume":"41","author":"Ding","year":"2011","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/S0965-1748(03)00002-X","article-title":"Polyunsaturated hydrocarbons in the hemolymph: Biosynthetic precursors of epoxy pheromones of geometrid and arctiid moths","volume":"33","author":"Wei","year":"2003","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1007\/s10886-007-9324-z","article-title":"Biosynthesis of (3Z,6Z,9Z)-3,6,9-Octadecatriene: The main component of the pheromone blend of Erannis bajaria","volume":"33","author":"Goller","year":"2007","journal-title":"J. Chem. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1146\/annurev-ento-031620-071754","article-title":"Chemical ecology, biochemistry, and molecular biology of insect hydrocarbons","volume":"66","author":"Blomquist","year":"2021","journal-title":"Annu. Rev. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e58019","DOI":"10.7554\/eLife.58019","article-title":"Isolation and transcriptomic analysis of Anopheles gambiae oenocytes enables the delineation of hydrocarbon biosynthesis","volume":"9","author":"Grigoraki","year":"2020","journal-title":"eLife"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s001140050511","article-title":"Novel and highly specific transport of a volatile sex pheromone by hemolymph lipophorin in moths","volume":"85","author":"Schal","year":"1998","journal-title":"Naturwissenschaften"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1016\/j.ibmb.2006.04.006","article-title":"Transport of a hydrophobic biosynthetic precursor by lipophorin in the hemolymph of a geometrid female moth which secretes an epoxyalkenyl sex pheromone","volume":"36","author":"Matsuoka","year":"2006","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ibmb.2019.03.002","article-title":"CYPs in different families are involved in the divergent regio-specific epoxidation of alkenyl sex pheromone precursors in moths","volume":"108","author":"Rong","year":"2019","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ibmb.2019.02.001","article-title":"Functional characterization of the epoxidase gene, Li_epo1 (CYP341B14), involved in generation of epoxyalkene pheromones in the mulberry tiger moth Lemyra imparilis","volume":"107","author":"Rong","year":"2019","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.ibmb.2014.09.009","article-title":"CYP341B14: A cytochrome P450 involved in the specific epoxidation of pheromone precursors in the fall webworm Hyphantria cunea","volume":"54","author":"Rong","year":"2014","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3389\/fphys.2018.01602","article-title":"Comparison of olfactory genes in two Ectropis species: Emphasis on candidates involved in the detection of Type-II sex pheromones","volume":"9","author":"Li","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_19","unstructured":"Ya-Nan, Z., Xiu-Yun, Z., Li-Ping, F., Peng, H., Zhi-Qiang, W., Geng, C., Liang, S., Zhan-Feng, Y., Dao-Gui, D., and Jin-Bu, L. (2015). Identification and expression profiles of sex pheromone biosynthesis and transport related genes in Spodoptera litura. PLoS ONE, 10."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wang, T., Liu, X., Luo, Z., Cai, X., Li, Z., Bian, L., Xiu, C., Chen, Z., Li, Q., and Fu, N. (2024). Transcriptome-wide identification of cytochrome P450s in tea black tussock moth (Dasychira baibarana) and candidate genes involved in Type-II sex pheromone biosynthesis. Insects, 15.","DOI":"10.3390\/insects15020139"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Yuan, T.-T., Luo, Z.-J., Luo, Z.-X., Cai, X.-M., Bian, L., Xiu, C.-L., Fu, N.-X., Chen, Z.-M., Zhang, L.-W., and Li, Z.-Q. (2022). Olfactory gene families in Scopula subpunctaria and candidates for Type-II sex pheromone detection. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232415775"},{"key":"ref_24","first-page":"40","article-title":"Standardization of qPCR and RT-qPCR","volume":"29","author":"Bustin","year":"2009","journal-title":"Genet. Eng. Biotechnol. News"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"14858","DOI":"10.1073\/pnas.1208650109","article-title":"An insect-specific P450 oxidative decarbonylase for cuticular hydrocarbon biosynthesis","volume":"109","author":"Qiu","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e12641","DOI":"10.7717\/peerj.12641","article-title":"Transcriptome analysis identifies candidate genes in the biosynthetic pathway of sex pheromones from a zygaenid moth, Achelura yunnanensis (Lepidoptera: Zygaenidae)","volume":"9","author":"Nuo","year":"2021","journal-title":"PeerJ"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1146\/annurev.ento.45.1.575","article-title":"Polyene hydrocarbons and epoxides: A second major class of lepidopteran sex attractant pheromones","volume":"45","author":"Millar","year":"2000","journal-title":"Annu. Rev. Entomol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1093\/jee\/tox216","article-title":"Evidence of premating isolation between two sibling moths: Ectropis grisescens and Ectropis obliqua (Lepidoptera: Geometridae)","volume":"110","author":"Luo","year":"2017","journal-title":"J. Econ. Entomol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1038\/s41437-020-00380-y","article-title":"Advances in deciphering the genetic basis of insect cuticular hydrocarbon biosynthesis and variation","volume":"126","author":"Holze","year":"2021","journal-title":"Heredity"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Moris, V.C., Podsiadlowski, L., Martin, S., Oeyen, J.P., Donath, A., Petersen, M., Wilbrandt, J., Misof, B., Liedtke, D., and Thamm, M. (2023). Intrasexual cuticular hydrocarbon dimorphism in a wasp sheds light on hydrocarbon biosynthesis genes in Hymenoptera. Commun. Biol., 6.","DOI":"10.1038\/s42003-022-04370-0"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4273","DOI":"10.1073\/pnas.0608142104","article-title":"A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogaster","volume":"104","author":"Chertemps","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/imb.12138","article-title":"Identification and characterization of a fatty acyl reductase from a Spodoptera littoralis female gland involved in pheromone biosynthesis","volume":"24","author":"Piulachs","year":"2015","journal-title":"Insect Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1515\/znc-2017-0031","article-title":"Biotechnological potential of insect fatty acid-modifying enzymes","volume":"72","author":"Tupec","year":"2017","journal-title":"Z. Naturforsch. C"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"29927","DOI":"10.1038\/srep29927","article-title":"Two fatty acyl reductases involved in moth pheromone biosynthesis","volume":"6","author":"Antony","year":"2016","journal-title":"Sci. Rep"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1111\/pbi.13906","article-title":"FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double-stranded FAR in rice exhibits strong pest resistance","volume":"20","author":"Sun","year":"2022","journal-title":"Plant Biotechnol. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"9156","DOI":"10.1073\/pnas.1531993100","article-title":"Pheromone gland-specific fatty-acyl reductase of the silkmoth, Bombyx mori","volume":"100","author":"Moto","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"8069","DOI":"10.1073\/pnas.1104355108","article-title":"Pheromone emergencies and drifting moth genomes","volume":"108","author":"Rooney","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"23592","DOI":"10.1074\/jbc.272.38.23592","article-title":"Molecular cloning and expression of fatty acid alpha-hydroxylase from Sphingomonas paucimobilis","volume":"272","author":"Matsunaga","year":"1997","journal-title":"J. Biol. Chem."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/15\/4\/276\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:28:20Z","timestamp":1760106500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/15\/4\/276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,15]]},"references-count":39,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["insects15040276"],"URL":"https:\/\/doi.org\/10.3390\/insects15040276","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,15]]}}}