{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T09:02:32Z","timestamp":1792054952887,"version":"4.4.0"},"reference-count":80,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS Kakenhi","award":["18H03960"],"award-info":[{"award-number":["18H03960"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Dietary management using immunostimulants to protect fish health and prevent bacterial infection is widely practiced. Many insect species possess various bioactive substances that can improve animal health. We previously identified several bioactive polysaccharides derived from insects, including dipterose-BSF from black soldier fly (Hermetia illucens) larvae; this can stimulate innate immunity in mammalian macrophage RAW264.7 cells. However, the effect of dietary dipterose-BSF on the immune system of teleosts remains unclear. Here, we analyzed the immune status of zebrafish (Danio rerio) after 14 days of dietary inclusion of dipterose-BSF (0.01, 0.1, and 1 \u00b5g\/g), followed by an immersion challenge using Edwardsiella tarda. To identify changes in the transcriptional profile induced by dipterose-BSF, we performed RNA-sequencing analyses of the liver and intestine. Differentially expressed genes were investigated, with both organs showing several upregulated genes, dominated by nuclear factor and tumor necrosis factor family genes. Gene Ontology analysis revealed several terms were significantly higher in the experimental group compared with the control group. Challenge tests suggested that dietary dipterose-BSF had some positive effects on disease resistance in fish, but these effects were not pronounced.<\/jats:p>","DOI":"10.3390\/insects15050326","type":"journal-article","created":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T10:33:31Z","timestamp":1714559611000},"page":"326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dietary Black Soldier Fly (Hermetia illucens)\u2014Dipterose-BSF\u2014Enhanced Zebrafish Innate Immunity Gene Expression and Resistance to Edwardsiella tarda Infection"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7408-081X","authenticated-orcid":false,"given":"Haruki","family":"Nishiguchi","sequence":"first","affiliation":[{"name":"Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2837-0911","authenticated-orcid":false,"given":"Ibnu Bangkit Bioshina","family":"Suryadi","sequence":"additional","affiliation":[{"name":"The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan"},{"name":"Department of Fisheries, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jalan Ir. Soekarno KM 21, Sumedang 45363, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0791-5732","authenticated-orcid":false,"given":"Muhammad Fariz Zahir","family":"Ali","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan"},{"name":"Research Center for Marine and Land Bio Industry, National Research and Innovation Agency, Jl. Raya Senggigi, Kodek Bay, Pemenang 83352, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiemi","family":"Miura","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2322-5301","authenticated-orcid":false,"given":"Takeshi","family":"Miura","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/j.1748-5967.2012.00465.x","article-title":"Antibacterial effect of extracts of Hermetia illucens (Diptera: Stratiomyidae) larvae against Gram-negative bacteria","volume":"42","author":"Choi","year":"2012","journal-title":"J. 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