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disciplines","award":["20225BCJ22005"],"award-info":[{"award-number":["20225BCJ22005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Huanglongbing (HLB) is a systemic plant disease caused by \u2018Candidatus Liberibacter asiaticus (CLas)\u2019 and transmitted by Diaphorina citri. D. citri acquires the CLas bacteria in the nymph stage and transmits it in the adult stage, indicating that molting from the nymph to adult stages is crucial for HLB transmission. However, the available D. citri reference genomes are incomplete, and gene function studies have been limited to date. In the current research, PacBio single-molecule real-time (SMRT) and Illumina sequencing were performed to investigate the transcriptome of D. citri nymphs and adults. In total, 10,641 full-length, non-redundant transcripts (FLNRTs), 594 alternative splicing (AS) events, 4522 simple sequence repeats (SSRs), 1086 long-coding RNAs (lncRNAs), 281 transcription factors (TFs), and 4459 APA sites were identified. Furthermore, 3746 differentially expressed genes (DEGs) between nymphs and adults were identified, among which 30 DEGs involved in the Hippo signaling pathway were found. Reverse transcription\u2013quantitative PCR (RT-qPCR) further validated the expression levels of 12 DEGs and showed a positive correlation with transcriptome data. Finally, the spatiotemporal expression pattern of genes involved in the Hippo signaling pathway exhibited high expression in the D. citri testis, ovary, and egg. Silencing of the D. citri transcriptional co-activator (DcYki) gene significantly increased D. citri mortality and decreased the cumulative molting. Our results provide useful information and a reliable data resource for gene function research of D. citri.<\/jats:p>","DOI":"10.3390\/insects15060391","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T03:48:49Z","timestamp":1716868129000},"page":"391","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Combinatorial Single-Molecule Real-Time and Illumina Sequencing Analysis of Postembryonic Gene Expression in the Asian Citrus Psyllid Diaphorina citri"],"prefix":"10.3390","volume":"15","author":[{"given":"Qin","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Can","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"He","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao-Ying","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Xin","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"},{"name":"National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-6312","authenticated-orcid":false,"given":"Zhan-Jun","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"},{"name":"National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8908-6781","authenticated-orcid":false,"given":"Hai-Zhong","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Gannan Normal University, Ganzhou 341000, China"},{"name":"National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"104036","DOI":"10.1016\/j.jprot.2021.104136","article-title":"Proteomic and transcriptomic analyses of saliva and salivary glands from the Asian citrus psyllid, Diaphorina citri","volume":"238","author":"Wu","year":"2021","journal-title":"J. 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