{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:37:16Z","timestamp":1793119036641,"version":"4.5.0"},"reference-count":84,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["31860612"],"award-info":[{"award-number":["31860612"]}]},{"name":"National Key Research and Development Program of China","award":["32070472"],"award-info":[{"award-number":["32070472"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Eulophidae and Pteromalidae are parasitic wasps with a global distribution and import for the biological control of pests. They can be distributed in different altitude regions, but their morphological and genetic adaptations to different altitudes are unclear. Here, we collected specimens that belong to Eulophidae and Pteromalidae from various altitudinal gradients, based on integrated taxonomic approaches to determine the species composition, and we analyzed their body shape and size from different altitudes using geometric morphometrics. Then, we performed an analysis of the D. isaea population\u2019s haplotype genes to illustrate their genetic diversity. As a result, eight species that belong to two genera, Diglyphus Walker (Eulophidae) and Pachyneuron Walker (Pteromalidae), were identified, including two newly recorded species from China (D. chabrias and D. sabulosus). Through a geometric morphometrics analysis of body shape, we found that a narrow forewing shape and a widened thorax are the significant characteristics of adaptation to high-altitude environments in D. isaea and P. aphidis. Additionally, the body size studies showed a principal relationship between centroid size and altitude; the size of the forewings and thorax increases at higher altitudes. Next, using haplotype analysis, 32 haplotypes were found in seven geographic populations with high genetic diversity of this species. Our research provides preliminary evidence for the morphological and genetic diversity adaptation of parasitic wasps to extreme environments, and these data can provide important references for investigations on the ecological adaptability of parasitic wasps.<\/jats:p>","DOI":"10.3390\/insects15070497","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T04:23:43Z","timestamp":1719980623000},"page":"497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Geometric Morphometrics and Genetic Diversity Analysis of Chalcidoidea (Diglyphus and Pachyneuron) at Various Elevations"],"prefix":"10.3390","volume":"15","author":[{"given":"Ouyan","family":"Xi","sequence":"first","affiliation":[{"name":"College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuli","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinzhe","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2295-1072","authenticated-orcid":false,"given":"Hongying","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Xinjiang University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Bai","sequence":"additional","affiliation":[{"name":"Institute of Zoology, Chinese Academy of Sciences, Beijing 100864, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1146\/annurev.anthro.34.081804.120613","article-title":"Geometric Morphometrics","volume":"36","author":"Slice","year":"2007","journal-title":"Annu. Rev. Anthropol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.16970\/entoted.989659","article-title":"Size and shape variations in wing morphology of Anopheles maculipennis s.s. Meigen, 1818 (Diptera: Culicidae) from northeastern Turkey","volume":"45","year":"2022","journal-title":"Turk. J. Entomol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Martinet, J.P., Ferte, H., Sientzoff, P., Krupa, E., Mathieu, B., and Depaquit, J. (2021). Wing Morphometrics of Aedes Mosquitoes from North-Eastern France. Insects, 12.","DOI":"10.3390\/insects12040341"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Simoes, R.F., Wilke, A.B.B., Chagas, C.R.F., Menezes, R.M.T., Suesdek, L., Multini, L.C., Silva, F.S., Grech, M.G., Marrelli, M.T., and Kirchgatter, K. (2020). Wing Geometric Morphometrics as a Tool for the Identification of Culex Subgenus Mosquitoes of Culex (Diptera: Culicidae). Insects, 11.","DOI":"10.3390\/insects11090567"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lao, J.S., Chang, B.J., and Chaiphongpachara, T. (2023). Evaluation of modern techniques for species identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric morphometrics and DNA barcoding. Insects, 14.","DOI":"10.3390\/insects14010078"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1080\/14772019.2022.2067491","article-title":"Systematic significance of wing morphology in extinct Prophalangopsidae (Insecta, Ensifera) revealed by geometric morphometrics and description of two new species","volume":"19","author":"Gu","year":"2022","journal-title":"J. Syst. Palaeontol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1111\/ele.12687","article-title":"Body size phenology in a regional bee fauna: A temporal extension of Bergmann\u2019s rule","volume":"19","author":"Arnan","year":"2016","journal-title":"Ecol. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s00442-018-4154-y","article-title":"Is thermal limitation the primary driver of elevational distributions? Not for montane rainforest ants in the Australian Wet Tropics","volume":"188","author":"Nowrouzi","year":"2018","journal-title":"Oecologia"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s00040-020-00796-z","article-title":"Ecogeographical patterns of body size differ among North American paper wasp species","volume":"68","author":"Miller","year":"2021","journal-title":"Insectes Sociaux"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4612","DOI":"10.1038\/s41467-019-12655-y","article-title":"Reduced body sizes in climate-impacted Borneo moth assemblages are primarily explained by range shifts","volume":"10","author":"Wu","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8936","DOI":"10.1002\/ece3.6596","article-title":"Temperature-driven color lightness and body size variation scale to local assemblages of European Odonata but are modified by propensity for dispersal","volume":"10","author":"Brandle","year":"2020","journal-title":"Ecol. Evol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jcz.2023.04.001","article-title":"A geometric morphometrics and genetics characterization of Vanessa carye in an extreme elevational gradient in the Chilean Altiplano","volume":"304","author":"Fabres","year":"2023","journal-title":"Zool. Anz."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kang, N., Hu, H.Y., Huang, Z.Q., Luo, S.G., and Guo, S.H. (2023). Environmental factors drive Chalcid body size increases with altitudinal gradients for two hyper-diverse taxa. Insects, 14.","DOI":"10.3390\/insects14010067"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/eea.13432","article-title":"Does the temperature\u2013size rule apply to idiobiont parasitoids?","volume":"172","author":"Gagnon","year":"2024","journal-title":"Entomol. Exp. Appl."},{"key":"ref_15","first-page":"595","article-title":"Morphometric variation in the wing of four populations of Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) in Iran","volume":"15","author":"Farsi","year":"2020","journal-title":"Munis Entomol. Zool."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1111\/1749-4877.12564","article-title":"Functional correlates of skull shape in Chiroptera: Feeding and echolocation adaptations","volume":"17","author":"Giacomini","year":"2022","journal-title":"Integr. Zool."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"32680","DOI":"10.1038\/srep32680","article-title":"Geographic variation in wing size and shape of the grasshopper Trilophidia annulata (Orthoptera: Oedipodidae): Morphological trait variations follow an ecogeographical rule","volume":"6","author":"Bai","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Nan, N.L., Hua, M.L., Yan, J., Xin, G.L., Yang, L., Tian, J.W., Jin, M.H., Qing, S.N., and Xiu, M.X. (2022). Geometric morphology and population genomics provide insights into the adaptive evolution of Apis cerana in Changbai Mountain. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08298-x"},{"key":"ref_19","first-page":"453","article-title":"The evolution of genetic diversity","volume":"205","author":"Clarke","year":"1997","journal-title":"Proc. R. Soc. Lond. Ser. B Biol. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Seyahooei, M.A., Alphen, J.J., and Kraaijeveld, K. (2011). Genetic structure of Leptopilina boulardi populations from different climatic zones of Iran. BMC Ecol., 11.","DOI":"10.1186\/1472-6785-11-4"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s11103-022-01326-0","article-title":"Genetic diversity and gene expression diversity shape the adaptive pattern of the aquatic plant Batrachium bungei along an altitudinal gradient on the Qinghai\u2013Tibet plateau","volume":"111","author":"Yu","year":"2022","journal-title":"Plant Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s13355-023-00840-x","article-title":"Globular galls on Haloxylon (Centrospermae: Chenopodiaceae) induced by gall midges, and the corresponding parasitoids in China","volume":"58","author":"Zhao","year":"2023","journal-title":"Appl. Entomol. Zool."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.biocontrol.2006.04.008","article-title":"Mitochondrial phylogeography of a leafminer parasitoid, Diglyphus isaea (Hymenoptera: Eulophidae) in China","volume":"38","author":"Sha","year":"2006","journal-title":"Biol. Control"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1080\/00305316.2009.10417593","article-title":"Pachyneuron (Hymenoptera: Pteromalidae) from China","volume":"43","author":"Xiao","year":"2009","journal-title":"Orient. Insects"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"DeSalle, R., Munro, J.B., Heraty, J.M., Burks, R.A., Hawks, D., Mottern, J., Cruaud, A., Rasplus, J.Y., and Jansta, P. (2011). A Molecular Phylogeny of the Chalcidoidea (Hymenoptera). PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027023"},{"key":"ref_26","first-page":"29","article-title":"The Australian species of Pachyneuron Walker (Hymenoptera: Chalcidoidea: Pteromalidae)","volume":"10","author":"Gibson","year":"2001","journal-title":"J. Hymenopt. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1016\/j.ijbiomac.2020.01.308","article-title":"Novel gene rearrangement in the mitochondrial genome of Pachyneuron aphidis (Hymenoptera: Pteromalidae)","volume":"149","author":"Wu","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Li, J.H., Wu, Y.K., Zhang, Q., Li, H.Q., Pan, H.S., Lu, W., Wang, D.M., Zhang, J.P., and Lu, Y.H. (2018). Aphid parasitism and parasitoid diversity in cotton fields in Xinjiang, China. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0207034"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1002\/jemt.23347","article-title":"Morphological analysis of sensilla on different organs in Pachyneuron aphidis, a hyperparasitoid of Myzus persicae","volume":"82","author":"Shen","year":"2019","journal-title":"Microsc. Res. Tech."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1365-3113.2010.00558.x","article-title":"Integrative taxonomy, or iterative taxonomy?","volume":"36","author":"Yeates","year":"2010","journal-title":"Syst. Entomol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/s13744-020-00834-2","article-title":"An Integrative Taxonomy of a New Species of Trichogramma Westwood (Hymenoptera: Trichogrammatidae) with High Reproductive Capacity","volume":"50","author":"Takahashi","year":"2021","journal-title":"Neotrop. Entomol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1742-9994-7-16","article-title":"The integrative future of taxonomy","volume":"7","author":"Padial","year":"2010","journal-title":"Front. Zool."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"103957","DOI":"10.1016\/j.earscirev.2022.103957","article-title":"Recent climate and hydrological changes in a mountain\u2013basin system in Xinjiang, China","volume":"226","author":"Yao","year":"2022","journal-title":"Earth-Sci. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.scitotenv.2019.04.297","article-title":"Identifying climate change impacts on water resources in Xinjiang, China","volume":"676","author":"Luo","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_35","unstructured":"Graham, M.W.R. (1987). A Reclassfication of the European Tetrastichinae (Hymenoptera: Eulophidae), with a Revision of Certain Genera, British Museum Press."},{"key":"ref_36","unstructured":"Gibson, G.A.P., Huber, J.T., and Woolley, J.B. (1997). Annotated Keys to the Genera of Nearctic Chalcidoidea (Hymenoptera), NRC Research Press."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1080\/00305316.2000.10417266","article-title":"A review of the Chinese Diglyphus Walker (Hymenoptera: Eulophidae)","volume":"34","author":"Zhu","year":"2000","journal-title":"Orient. Insects"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/j.1439-0469.2006.00375.x","article-title":"Diglyphus isaea (Hymenoptera: Eulophidae): A probable complex of cryptic species that forms an important biological control agent of agromyzid leaf miners","volume":"45","author":"Sha","year":"2007","journal-title":"J. Zool. Syst. Evol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"73","DOI":"10.31610\/zsr\/2009.18.1.73","article-title":"Review of Kazakhstan species of the genus Pachyneuron Walker, 1833 (Hymenoptera: Chalcidoidea: Pteromalidae)","volume":"18","author":"Dzhanokmen","year":"2009","journal-title":"Zoosyst. Ross."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"197","DOI":"10.11646\/zootaxa.4269.2.2","article-title":"Review of the European species of Diglyphus Walker (Hymenoptera: Eulophidae) including the description of a new species","volume":"4269","author":"Hansson","year":"2017","journal-title":"Zootaxa"},{"key":"ref_41","first-page":"9","article-title":"The tps series of software","volume":"26","author":"Rohlf","year":"2015","journal-title":"Hystrix Ital. J. Mammal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/j.1755-0998.2010.02924.x","article-title":"MorphoJ: An integrated software package for geometric morphometrics","volume":"11","author":"Klingenberg","year":"2010","journal-title":"Mol. Ecol. Resour."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1111\/syen.12081","article-title":"Morphometric analysis and taxonomic revision of Anisopteromalus Ruschka (Hymenoptera: Chalcidoidea: Pteromalidae)\u2014An integrative approach","volume":"39","author":"Baur","year":"2014","journal-title":"Syst. Entomol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1093\/nar\/22.22.4673","article-title":"Clustal w: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e87","DOI":"10.1002\/imt2.87","article-title":"Using PhyloSuite for molecular phylogeny and tree-based analyses","volume":"16","author":"Xiang","year":"2023","journal-title":"iMeta"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"Modelfinder: Fast model selection for accurate phylogenetic estimates","volume":"14","author":"Kalyaanamoorthy","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hoang, D.T., Vinh, L.S., Flouri, T., Stamatakis, A., von Haeseler, A., and Minh, B.Q. (2018). MPBoot: Fast phylogenetic maximum parsimony tree inference and bootstrap approximation. BMC Evol. Biol., 18.","DOI":"10.1186\/s12862-018-1131-3"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.1111\/j.1365-294X.2011.05239.x","article-title":"ABGD, Automatic Barcode Gap Discovery for primary species delimitation","volume":"21","author":"Puillandre","year":"2012","journal-title":"Mol. Ecol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s00239-018-9885-1","article-title":"An extension of the Kimura two-parameter model to the natural evolutionary process","volume":"87","author":"Nishimaki","year":"2019","journal-title":"J. Mol. Evol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/2041-210X.12035","article-title":"Geomorph: An r package for the collection and analysis of geometric morphometric shape data","volume":"4","author":"Adams","year":"2013","journal-title":"Methods Ecol. Evol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1590\/S1415-47572013000400002","article-title":"Mantel test in population genetics","volume":"36","author":"Soares","year":"2013","journal-title":"Genet. Mol. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Luo, Z., Brock, J., Dyer, J.M., Kutchan, T., Schachtman, D., Augustin, M., Ge, Y., Fahlgren, N., and Abdel-Haleem, H. (2019). Genetic Diversity and Population Structure of a Camelina sativa Spring Panel. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00184"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1093\/jhered\/93.2.153","article-title":"IBD (isolation by distance): A program for analyses of isolation by distance","volume":"93","author":"Bohonak","year":"2002","journal-title":"J. Hered."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1080\/00222933.2020.1762941","article-title":"A first phylogenomic hypothesis for Eulophidae (Hymenoptera, Chalcidoidea)","volume":"54","author":"Rasplus","year":"2020","journal-title":"J. Nat. Hist."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1080\/00379271.2011.10697720","article-title":"A review of Turkish DiglyphusWalker (Hymenoptera: Eulophidae), with description of a new species","volume":"47","author":"Yefremova","year":"2013","journal-title":"Ann. Soc. Entomol. Fr. (NS)"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3897\/jhr.52.8787","article-title":"Geometric morphometric discrimination of the three African honeybee subspecies Apis mellifera intermissa, A. m. sahariensis and A. m. capensis (Hymenoptera, Apidae): Fore wing and hind wing landmark configurations","volume":"52","author":"Barour","year":"2016","journal-title":"J. Hymenopt. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"01205","DOI":"10.1088\/1755-1315\/197\/1\/012015","article-title":"Honey bees species differentiation using geometric morphometric on wing venations","volume":"197","author":"Santoso","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1080\/10635150590947258","article-title":"Distances and directions in multidimensional shape spaces: Implications for morphometric applications","volume":"54","author":"Peter","year":"2005","journal-title":"Syst. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s00427-016-0539-2","article-title":"Size, shape, and form: Concepts of allometry in geometric morphometrics","volume":"226","author":"Klingenberg","year":"2016","journal-title":"Dev. Genes Evol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1186\/s13071-017-2163-z","article-title":"Wing morphometrics as a tool in species identification of forensically important blow flies of Thailand","volume":"10","author":"Sontigun","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Ma, Y., Guo, Z., Wang, L., Wang, B., Huang, T., Tang, B., Zhang, G., and Zhou, Q. (2022). The genome of the rice planthopper egg parasitoid wasps Anagrus nilaparvatae casts light on the chemo- and mechanosensation in parasitism. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08656-9"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Tang, L.D., Sun, J.W., Dai, P., Mu, M.Y., Nkunika, P.O.Y., Desneux, N., and Zang, L.S. (2023). Performance of two dominant Trichogrammatid species of fall armyworm from China and Africa under contrasted temperature and humidity regimes. Biol. Control, 179.","DOI":"10.1016\/j.biocontrol.2023.105179"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"14622","DOI":"10.1038\/s41598-022-18891-5","article-title":"Seasonal variation in wing size and shape of Drosophila melanogaster reveals rapid adaptation to environmental changes","volume":"12","author":"Aksoy","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Wallberg, A., Schoning, C., Webster, M.T., and Hasselmann, M. (2017). Two extended haplotype blocks are associated with adaptation to high altitude habitats in East African honey bees. PLoS Genet., 13.","DOI":"10.1371\/journal.pgen.1006792"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"10851","DOI":"10.1038\/ncomms10851","article-title":"Enhanced flight performance by genetic manipulation of wing shape in Drosophila","volume":"7","author":"Ray","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_68","first-page":"140","article-title":"Morphologic differentiation of the exotic parasitoid Eupelmus pulchriceps (Hymenoptera: Eupelmidae) in the Galapagos Archipelago","volume":"53","author":"Herrera","year":"2024","journal-title":"Neotrop. Entomol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Anand, P.P., Seena, S., Girish, K.P., and Shibu, V.Y. (2023). Species morphospace boundary revisited through wing phenotypic variations of Antodynerus species (Hymenoptera: Vespidae: Eumeninae) from the Indian subcontinent. Front. Ecol. Evol., 10.","DOI":"10.3389\/fevo.2022.965577"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Benitez, H.A., Munoz-Ramirez, C., Correa, M., Acuna-Rodriguez, I.S., Villalobos-Leiva, A., Contador, T., Velasquez, N.A., and Suazo, M.J. (2024). Breaking the law: Is it correct to use the converse Bergmann Rule in ceroglossus chilensis? An overview using geometric morphometrics. Insects, 15.","DOI":"10.3390\/insects15020097"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1111\/een.12853","article-title":"Temperature-body size responses in insects: A case study of British Odonata","volume":"45","author":"Wonglersak","year":"2020","journal-title":"Ecol. Entomol."},{"key":"ref_72","first-page":"1","article-title":"Divergence in body mass, wing loading, and population structure reveals species-specific and potentially adaptive trait variation across elevations in montane bumble bees","volume":"5","author":"Lozier","year":"2021","journal-title":"Insect Syst. Divers."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1007\/s13744-020-00784-9","article-title":"Relationship between body mass and forewing length in neotropical Ichneumonidae (Insecta: Hymenoptera)","volume":"49","author":"Mazon","year":"2020","journal-title":"Neotrop. Entomol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.cub.2017.01.027","article-title":"Evolutionary history of the hymenoptera","volume":"27","author":"Peters","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_75","first-page":"1","article-title":"Altitudinal distribution and body resource allocation in a high mountain social wasp (Hymenoptera: Vespidae)","volume":"37","author":"Sarmiento","year":"2008","journal-title":"Ecol. Behav. Bionomics"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/ens.12293","article-title":"Geometric morphometrics in entomology: Basics and applications: Geometric morphometrics in entomology","volume":"21","author":"Tatsuta","year":"2017","journal-title":"Entomol. Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s10974-011-9278-1","article-title":"Regulating the contraction of insect flight muscle","volume":"32","author":"Bullard","year":"2011","journal-title":"J. Muscle Res. Cell Motil."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1242\/jeb.128363","article-title":"Mechanics of the thorax in flies","volume":"220","author":"Deora","year":"2017","journal-title":"J. Exp. Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"367","DOI":"10.16970\/entoted.331102","article-title":"Morphometric analysis of wild-caught flies of Drosophila (Diptera: Drosophilidae) species: Altitudinal pattern of body size traits, wing morphology and sexual dimorphism","volume":"41","author":"Fartyal","year":"2018","journal-title":"Turk. J. Entomol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Cao, T., and Jin, J.P. (2020). Evolution of flight muscle contractility and energetic efficiency. Front. Physiol., 11.","DOI":"10.3389\/fphys.2020.01038"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.ejmp.2021.07.005","article-title":"Advances in micro-CT imaging of small animals","volume":"88","author":"Clark","year":"2021","journal-title":"Phys. Med."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1088\/0031-9155\/53\/19\/R01","article-title":"In vivo small-animal imaging using micro-CT and digital subtraction angiography","volume":"53","author":"Badea","year":"2008","journal-title":"Phys. Med. Biol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2478\/dna-2014-0001","article-title":"Mitochondrial markers for identification and phylogenetic studies in insects\u2014A Review","volume":"2","author":"Mandal","year":"2014","journal-title":"DNA Barcodes"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/een.12792","article-title":"How climate change affects the seasonal ecology of insect parasitoids","volume":"45","author":"Tougeron","year":"2020","journal-title":"Ecol. Entomol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/15\/7\/497\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:09:28Z","timestamp":1760108968000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/15\/7\/497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,3]]},"references-count":84,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["insects15070497"],"URL":"https:\/\/doi.org\/10.3390\/insects15070497","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,3]]}}}