{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:06:46Z","timestamp":1788311206997,"version":"build-2803163510"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T00:00:00Z","timestamp":1722729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Guangxi Key Laboratory of Sugarcane Genetic Improvement\/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning","award":["21-238-16-K-05-02"],"award-info":[{"award-number":["21-238-16-K-05-02"]}]},{"name":"Project of Guangxi Key Laboratory of Sugarcane Genetic Improvement\/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning","award":["31972274"],"award-info":[{"award-number":["31972274"]}]},{"name":"Project of Guangxi Key Laboratory of Sugarcane Genetic Improvement\/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning","award":["T2022009"],"award-info":[{"award-number":["T2022009"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21-238-16-K-05-02"],"award-info":[{"award-number":["21-238-16-K-05-02"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972274"],"award-info":[{"award-number":["31972274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["T2022009"],"award-info":[{"award-number":["T2022009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Outstanding Youth Science and Technology Innovation Team Project of Colleges and Universities in Hubei Province","award":["21-238-16-K-05-02"],"award-info":[{"award-number":["21-238-16-K-05-02"]}]},{"name":"Outstanding Youth Science and Technology Innovation Team Project of Colleges and Universities in Hubei Province","award":["31972274"],"award-info":[{"award-number":["31972274"]}]},{"name":"Outstanding Youth Science and Technology Innovation Team Project of Colleges and Universities in Hubei Province","award":["T2022009"],"award-info":[{"award-number":["T2022009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Chilo sacchariphagus (Lepidoptera: Pyralidae) is an economically important sugarcane pest. Although numerous studies were conducted on the physiological responses in C. sacchariphagus, little is known regarding the genes regulating these physiological processes. Gene expression analysis by qRT-PCR can offer a significant indication for functional gene studies. To our knowledge, the reference genes of C. sacchariphagus have not been screened or evaluated, which hinders the functional gene study. In the present study, the stability of seven reference genes (\u03b2-ACT, GAPDH, BTF3, 28S, RPL7, EF1\u03b1, and SDHA) was evaluated in C. sacchariphagus under different experimental conditions, including tissues (antenna, head, thorax, abdomen, leg, and wing), temperatures (4 \u00b0C, 25 \u00b0C, and 37 \u00b0C) and sexes (male and female), through RefFinder, which integrates four algorithms (Normfinder, BestKeeper, \u0394Ct method, and geNorm). The findings suggested that the combination of \u03b2-ACT and RPL7 is ideal to analyze gene expressions in different tissues and at distinct temperatures, and EF1\u03b1 and SDHA were suitable reference genes for comparing gene expressions between sexes. Finally, the expression profiles of CsacPBP1 gene were evaluated, and the outcomes further confirm the importance of selecting fitting reference genes for normalization of qRT-PCR data. This study represents the first kind in screening out suitable reference genes for gene expression analysis in C. sacchariphagus. Information from this study is poised to galvanize future inquiry into the gene expression of C. sacchariphagus, an economically important pest of sugarcane.<\/jats:p>","DOI":"10.3390\/insects15080594","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T13:57:28Z","timestamp":1722866248000},"page":"594","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation and Validation of Reference Genes for Gene Expression Analysis Using qRT-PCR in the Sugarcane Stem Borer Chilo sacchariphagus (Lepidoptera: Pyralidae)"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhixiong","family":"Wang","sequence":"first","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8823-7798","authenticated-orcid":false,"given":"Xiankun","family":"Shang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs\/Guangxi Key Laboratory of Sugarcane Genetic Improvement\/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jili","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs\/Guangxi Key Laboratory of Sugarcane Genetic Improvement\/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Life Science, Yangtze University, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agriculture, Yangtze University, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.cropro.2012.06.003","article-title":"The use of Erianthus arundinaceus as a trap crop for the stem borer Chilo sacchariphagus reduces yield losses in sugarcane: Preliminary results","volume":"42","author":"Nibouche","year":"2012","journal-title":"Crop Prot."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"104877","DOI":"10.1016\/j.cropro.2019.104877","article-title":"Erianthus arundinaceus as a trap crop for the sugarcane stem borer Chilo sacchariphagus: Field validation and disease risk assessment","volume":"124","author":"Nibouche","year":"2019","journal-title":"Crop Prot."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Li, A., Chen, Z., Liao, F., Zhao, Y., Qin, C., Wang, M., Pan, Y., Wei, S., and Huang, D. 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