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Factors such as environmental conditions and genetic variations in the yellow fever virus (YFV) strains and mosquito populations may explain this absence. Mosquito populations have undergone strong selective pressure owing to the excessive use of insecticides. This pressure has led to the spread of alterations, such as knockdown-resistant mutations (kdr), which, while conferring resistance to pyrethroids, also induce various physiological side effects in the insect. Therefore, it is important to investigate whether the presence of kdr mutations influences the infectivity of YFV mosquitoes. This study evaluated the susceptibility of Ae. aegypti from Pakistan with distinct kdr genotypes to different YFV strains under laboratory conditions. Methods: Ae. aegypti from a Pakistani colony were exposed to YFV strains (PR4408\/2008 and ES504\/2017) along with the Rockefeller strain. After 14 days, RNA and DNA were extracted for viral RNA detection (qPCR) and kdr genotyping (TaqMan qPCR and HRM for T1520I and F1534C SNPs). Results: Pakistani Ae. aegypti were orally susceptible to YFV, with infection rates of 83.7% (PR4408\/2008) and 61.3% (ES504), respectively, similar to Rockefeller. Two kdr genotypes (II + CC and TI + FC) were identified, with no significant differences in viral infection or dissemination rates. Conclusions: The Ae. aegypti population from Asia is capable of YFV infection and dissemination, regardless of kdr genotype.<\/jats:p>","DOI":"10.3390\/insects16010033","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T12:37:19Z","timestamp":1735648639000},"page":"33","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Yellow Fever Virus Infection in Aedes aegypti Mosquitoes from Pakistan with Distinct Knockdown Resistance Genotypes"],"prefix":"10.3390","volume":"16","author":[{"given":"Carlucio Rocha","family":"dos Santos","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Controlee Vigil\u00e2ncia de InsetosVetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2700-0049","authenticated-orcid":false,"given":"Caleb Guedes Miranda dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Instituto de Biologia do Ex\u00e9rcito, Divis\u00e3o de Ensino e Pesquisa, Rio de Janeiro 20911-270, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2043-6306","authenticated-orcid":false,"given":"Dinair","family":"Couto-Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Mosquitos Transmissores de Hematozo\u00e1rios, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara Silva","family":"Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Controlee Vigil\u00e2ncia de InsetosVetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-0453","authenticated-orcid":false,"given":"Rafi Ur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Controlee Vigil\u00e2ncia de InsetosVetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Dornelas Ribeiro","sequence":"additional","affiliation":[{"name":"Instituto de Biologia do Ex\u00e9rcito, Divis\u00e3o de Ensino e Pesquisa, Rio de Janeiro 20911-270, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Bento Pereira","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Controlee Vigil\u00e2ncia de InsetosVetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5739-1215","authenticated-orcid":false,"given":"Ademir Jesus","family":"Martins","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Controlee Vigil\u00e2ncia de InsetosVetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia (INCT), Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow fever","volume":"64","author":"Monath","year":"2015","journal-title":"J. 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