{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T05:51:36Z","timestamp":1788846696063,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T00:00:00Z","timestamp":1740873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research and Development Program sponsored by the Ministry of Science and Technology (MOST)","award":["2023YFA1801004"],"award-info":[{"award-number":["2023YFA1801004"]}]},{"name":"Key Research and Development Program sponsored by the Ministry of Science and Technology (MOST)","award":["82261128002"],"award-info":[{"award-number":["82261128002"]}]},{"name":"National Natural Science Foundation of China, and the Bill and Melinda Gates Foundation joint program","award":["2023YFA1801004"],"award-info":[{"award-number":["2023YFA1801004"]}]},{"name":"National Natural Science Foundation of China, and the Bill and Melinda Gates Foundation joint program","award":["82261128002"],"award-info":[{"award-number":["82261128002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Aedes albopictus is one of the most important vectors of Dengue, which poses a serious threat to public health. The bacterial microbiota has an effect on the parameters of mosquitos, such as larval development and fecundity, and it has emerged as a promising field to be explored for novel environmentally friendly control strategies. Rahnella sp. are present in many insects, including Ae. Albopictus, and play a role in bacterial\u2013insect interactions; however, the role of the bacteria in mosquito biology has not yet been characterized. In this study, we characterized the Rahnella isolate RAeA1 obtained from Ae. albopcitus, and its colonization stability in Ae. albopictus was investigated by generating GFP-tagged bacteria. The influences of the bacteria on larval development and mosquito reproductive capacity were evaluated by inoculating RAeA1 in axenic larvae and antibiotic-treated adult mosquitoes, respectively. The results indicated that RAeA1, which is widespread in the field population of Ae. albopictus, can be transmitted directly from the parental strain to the progeny and can rescue axenic larvae developing into adults with a prolonged development time to pupation. RAeA1 inoculation can impair egg production and ovary maturation, as well as reducing the synthesis of ecdysteroids and vitellogenin in Ae. albopictus females. Overall, our results provide a thorough study of bacterium function characterization that will facilitate the development of potential strategies in relation to the design of microbiomes for vector control.<\/jats:p>","DOI":"10.3390\/insects16030257","type":"journal-article","created":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T05:52:16Z","timestamp":1740981136000},"page":"257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Rahnella aquatilis Isolated from Aedes albopictus Impairs Mosquito Reproduction Capacity"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7377-7506","authenticated-orcid":false,"given":"Ling","family":"Gu","sequence":"first","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyang","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiao","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Wan","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meichun","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julin","family":"Li","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meihua","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoding","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7145-9922","authenticated-orcid":false,"given":"Jianxia","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Public Health, Nanjing Medical University, Nanjing 211166, China"},{"name":"National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Provincial Medical Key Laboratory, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Battaglia, V., Agostini, V., Moroni, E., Colombo, G., Lombardo, G., Rambaldi Migliore, N., Gabrieli, P., Garofalo, M., Gagliardi, S., and Gomulski, L.M. (2022). The worldwide spread of Aedes albopictus: New insights from mitogenomes. Front. Genet., 13.","DOI":"10.3389\/fgene.2022.931163"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.pt.2013.07.003","article-title":"The invasive mosquito species Aedes albopictus: Current knowledge and future perspectives","volume":"29","author":"Bonizzoni","year":"2013","journal-title":"Trends Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1186\/s13071-015-0959-2","article-title":"Paratransgenesis: A promising new strategy for mosquito vector control","volume":"8","author":"Wilke","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.mib.2012.03.004","article-title":"Mosquito\/microbiota interactions: From complex relationships to biotechnological perspectives","volume":"15","author":"Ricci","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.cois.2018.05.008","article-title":"Composition and functional roles of the gut microbiota in mosquitoes","volume":"28","author":"Strand","year":"2018","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1002\/ps.5643","article-title":"A bacterium against the tiger: Preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area","volume":"76","author":"Caputo","year":"2020","journal-title":"Pest. Manag. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1186\/s13071-016-1660-9","article-title":"Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae)","volume":"9","author":"Coon","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Scolari, F., Casiraghi, M., and Bonizzoni, M. (2019). Aedes spp. and Their Microbiota: A Review. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02036"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Chen, S., Zhang, D., Augustinos, A., Doudoumis, V., Bel Mokhtar, N., Maiga, H., Tsiamis, G., and Bourtzis, K. (2020). Multiple Factors Determine the Structure of Bacterial Communities Associated With Aedes albopictus Under Artificial Rearing Conditions. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00605"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Saab, S.A., Dohna, H.Z., Nilsson, L.K.J., Onorati, P., Nakhleh, J., Terenius, O., and Osta, M.A. (2020). The environment and species affect gut bacteria composition in laboratory co-cultured Anopheles gambiae and Aedes albopictus mosquitoes. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-60075-6"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.meegid.2014.09.029","article-title":"Composition of Anopheles coluzzii and Anopheles gambiae microbiota from larval to adult stages","volume":"28","author":"Gimonneau","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1111\/mec.12771","article-title":"Mosquitoes rely on their gut microbiota for development","volume":"23","author":"Coon","year":"2014","journal-title":"Mol. Ecol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gu\u00e9gan, M., Zouache, K., D\u00e9michel, C., Minard, G., Tran Van, V., Potier, P., Mavingui, P., and Valiente Moro, C. (2018). The mosquito holobiont: Fresh insight into mosquito-microbiota interactions. Microbiome, 6.","DOI":"10.1186\/s40168-018-0435-2"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yadav, K.K., Datta, S., Naglot, A., Bora, A., Hmuaka, V., Bhagyawant, S., Gogoi, H.K., Veer, V., and Raju, P.S. (2016). Diversity of Cultivable Midgut Microbiota at Different Stages of the Asian Tiger Mosquito, Aedes albopictus from Tezpur, India. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0167409"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.syapm.2014.09.001","article-title":"Rahnella victoriana sp. nov., Rahnella bruchi sp. nov., Rahnella woolbedingensis sp. nov., classification of Rahnella genomospecies 2 and 3 as Rahnella variigena sp. nov. and Rahnella inusitata sp. nov., respectively and emended description of the genus Rahnella","volume":"37","author":"Brady","year":"2014","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Wang, Y.H., Salam, N., Liu, Q., Yang, Z.W., Cao, L.X., Meng, X.L., Nie, G.X., Ju, J.H., and Li, W.J. (2017). Symbiotic bacteria associated with puffer fish Gastrophysus spadiceus and evaluation of their antimicrobial activities. 3 Biotech., 7.","DOI":"10.1007\/s13205-017-0989-x"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12223-016-0469-4","article-title":"Degradation capacities of bacteria and yeasts isolated from the gut of Dendroctonus rhizophagus (Curculionidae: Scolytinae)","volume":"62","year":"2017","journal-title":"Folia Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chakraborty, A., Ashraf, M.Z., Modlinger, R., Synek, J., Schlyter, F., and Roy, A. (2020). Unravelling the gut bacteriome of Ips (Coleoptera: Curculionidae: Scolytinae): Identifying core bacterial assemblage and their ecological relevance. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-75203-5"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Pineda-Mendoza, R.M., Z\u00fa\u00f1iga, G., L\u00f3pez, M.F., Hidalgo-Lara, M.E., Santiago-Hern\u00e1ndez, A., L\u00f3pez-L\u00f3pez, A., Ordu\u00f1a, F.N.R., and Cano-Ram\u00edrez, C. (2022). Rahnella sp., a Dominant Symbiont of the Core Gut Bacteriome of Dendroctonus Species, Has Metabolic Capacity to Degrade Xylan by Bifunctional Xylanase-Ferulic Acid Esterase. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.911269"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"745","DOI":"10.4014\/jmb.2301.01018","article-title":"Pantoea Bacteria Isolated from Three Thrips (Frankliniella occidentalis, Frankliniella intonsa, and Thrips tabaci) in Korea and Their Symbiotic Roles in Host Insect Development","volume":"33","author":"Jin","year":"2023","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.ijfoodmicro.2006.07.003","article-title":"Biological control of postharvest spoilage caused by Penicillium expansum and Botrytis cinerea in apple by using the bacterium Rahnella aquatilis","volume":"113","author":"Calvo","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mei, L., Xu, S., Lu, P., Lin, H., Guo, Y., and Wang, Y. (2017). CsrB a noncoding regulatory RNA, is required for BarA-dependent expression of biocontrol traits in Rahnella aquatilis HX2. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0187492"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"El Khoury, S., Giovenazzo, P., and Derome, N. (2022). Endogenous Honeybee Gut Microbiota Metabolize the Pesticide Clothianidin. Microorganisms, 10.","DOI":"10.3390\/microorganisms10030493"},{"key":"ref_24","first-page":"475","article-title":"Microbiota structure and diversity in Aedes albopictus at different developmental stages","volume":"34","author":"Zhao","year":"2022","journal-title":"Chin. J. Schistosomiasis Control"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"105174","DOI":"10.1016\/j.actatropica.2019.105174","article-title":"Use of MALDI-TOF MS to identify the culturable midgut microbiota of laboratory and wild mosquitoes","volume":"200","author":"Feitosa","year":"2019","journal-title":"Acta Trop."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1574-6941.2010.01012.x","article-title":"Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar","volume":"75","author":"Zouache","year":"2011","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Rose, W.A., McGowin, C.L., Spagnuolo, R.A., Eaves-Pyles, T.D., Popov, V.L., and Pyles, R.B. (2012). Commensal bacteria modulate innate immune responses of vaginal epithelial cell multilayer cultures. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0032728"},{"key":"ref_28","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol. Biol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (iTOL) v5: An online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lu, P., Bian, G., Pan, X., and Xi, Z. (2012). Wolbachia induces density-dependent inhibition to dengue virus in mosquito cells. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001754"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1038\/s41467-021-21195-3","article-title":"Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host-microbiota interactions","volume":"12","author":"Romoli","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.tim.2023.02.007","article-title":"Axenic and gnotobiotic insect technologies in research on host-microbiota interactions","volume":"31","author":"Wu","year":"2023","journal-title":"Trends Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Martinson, V.G., and Strand, M.R. (2021). Diet-Microbiota Interactions Alter Mosquito Development. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.650743"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Xi, Z., Ramirez, J.L., and Dimopoulos, G. (2008). The Aedes aegypti toll pathway controls dengue virus infection. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000098"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1534\/g3.114.016162","article-title":"Comparison of larval and adult Drosophila astrocytes reveals stage-specific gene expression profiles","volume":"5","author":"Huang","year":"2015","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Pang, X., Xiao, X., Liu, Y., Zhang, R., Liu, J., Liu, Q., Wang, P., and Cheng, G. (2016). Mosquito C-type lectins maintain gut microbiome homeostasis. Nat. Microbiol., 1.","DOI":"10.1038\/nmicrobiol.2016.23"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1186\/s13071-023-05957-0","article-title":"MicroRNA-989 targets 5-hydroxytryptamine receptor1 to regulate ovarian development and eggs production in Culex pipiens pallens","volume":"16","author":"Zheng","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Harrison, R.E., Yang, X., Eum, J.H., Martinson, V.G., Dou, X., Valzania, L., Wang, Y., Boyd, B.M., Brown, M.R., and Strand, M.R. (2023). The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence. Commun. Biol., 6.","DOI":"10.1038\/s42003-023-05545-z"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1007\/s00203-020-01961-5","article-title":"Rahnella aceris sp. nov., isolated from sap drawn from Acer pictum","volume":"202","author":"Lee","year":"2020","journal-title":"Arch. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Valzania, L., Martinson, V.G., Harrison, R.E., Boyd, B.M., Coon, K.L., Brown, M.R., and Strand, M.R. (2018). Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006638"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Coon, K.L., Valzania, L., Brown, M.R., and Strand, M.R. (2020). Predaceous Toxorhynchites mosquitoes require a living gut microbiota to develop. Proc. Biol. Sci., 287.","DOI":"10.1098\/rspb.2019.2705"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1007\/s00436-017-5466-2","article-title":"Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany","volume":"116","author":"Zotzmann","year":"2017","journal-title":"Parasitol. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Rani, A., Sharma, A., Rajagopal, R., Adak, T., and Bhatnagar, R.K. (2009). Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector. BMC Microbiol., 9.","DOI":"10.1186\/1471-2180-9-96"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1186\/1756-3305-7-419","article-title":"Isolation and identification of culturable bacteria from wild Anopheles culicifacies, a first step in a paratransgenesis approach","volume":"7","author":"Chavshin","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1186\/s13071-021-05093-7","article-title":"Characterization of the reproductive tract bacterial microbiota of virgin, mated, and blood-fed Aedes aegypti and Aedes albopictus females","volume":"14","author":"Camargo","year":"2021","journal-title":"Parasit. Vectors"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1111\/mec.16327","article-title":"Mosquito-bacteria interactions during larval development trigger metabolic changes with carry-over effects on adult fitness","volume":"31","author":"Giraud","year":"2022","journal-title":"Mol. Ecol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1186\/1756-3305-4-105","article-title":"Contribution of midgut bacteria to blood digestion and egg production in Aedes aegypti (diptera: Culicidae) (L.)","volume":"4","author":"Santos","year":"2011","journal-title":"Parasit. Vectors"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e01142-20","DOI":"10.1128\/mBio.01142-20","article-title":"Insect bacterial symbiont-mediated vitellogenin uptake into ooocytes to support egg development","volume":"11","author":"Mao","year":"2020","journal-title":"mBio"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Lau, M.J., Ross, P.A., Endersby-Harshman, N.M., Yang, Q., and Hoffmann, A.A. (2022). Wolbachia inhibits ovarian formation and increases blood feeding rate in female Aedes aegypti. PLoS Negl. Trop. Dis., 16.","DOI":"10.1101\/2022.04.19.488749"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1146\/annurev-ento-020117-043258","article-title":"Regulatory pathways controlling female insect reproduction","volume":"63","author":"Roy","year":"2018","journal-title":"Annu. Rev. Entomol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"15506","DOI":"10.1073\/pnas.0503501102","article-title":"Synergistic action of E74B and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene","volume":"102","author":"Sun","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_53","first-page":"S475","article-title":"Unusual functions of insect vitellogenins: Minireview","volume":"72","year":"2023","journal-title":"Physiol Res."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/3\/257\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:45:47Z","timestamp":1760028347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/3\/257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,2]]},"references-count":53,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["insects16030257"],"URL":"https:\/\/doi.org\/10.3390\/insects16030257","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,2]]}}}