{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:33:30Z","timestamp":1791689610383,"version":"4.3.4"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T00:00:00Z","timestamp":1746921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RUDN University Strategic Academic Leadership Program"},{"name":"Higher Education Commission (HEC), Pakistan"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Climate change is thought to be responsible for the spread of various vector-borne diseases. The current study was conducted to evaluate the impact of different temperature and relative humidity regimes on the developmental stages of the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae). The study also evaluated the impact of larval density on the survival of Ae. aegypti. In addition, the association between vector larval abundance, dengue incidence, and climatic factors were elucidated during 2016\u20132019 in three populated districts of Punjab, Pakistan, i.e., Lahore, Rawalpindi, and Multan. The results of the study revealed that at 10 \u00b0C and 35 \u00b0C, egg hatching and adult emergence were significantly reduced, regardless of the relative humidity. In contrast, at 20 \u00b0C and 30 \u00b0C, the rates of egg and adult survival increased with higher relative humidity. In addition, a density-dependent response was observed regarding larval survival of Ae. aegypti. Moreover, larval incidence was positively correlated with the number of dengue patients, Tmax, RH, and precipitation at Lahore (0.55, 0.23, 0.29, and 0.13), Rawalpindi (0.90, 0.30, 0.21, and 0.14), and Multan (0.05, 0.27, and 0.13) respectively, except in Multan, where a negative correlation (\u22120.09) with precipitation was observed. The inflow of patients had a positive correlation with the occurrence of a larval population, relative humidity, and precipitation at Lahore, Rawalpindi, and Multan districts, with the scale values of 0.55, 0.25, and 0.16; 0.90, 0.22, and 0.03; and 0.05, 0.06, and 0.03, respectively. In addition, a forecast model, ARIMA, predicted that there was a higher rate of larval occurrence in Rawalpindi, followed by Lahore. This study concluded that the role of precipitation &gt; 200 mm prior to a 1\u20132-month lag, a 20\u201330 \u00b0C temperature range, and an RH exceeding 60% lead to the occurrence of larvae and dengue case spikes. This study will help to reinforce dengue surveillance and control strategies in Pakistan and to establish early management strategies based on changing climatic factors.<\/jats:p>","DOI":"10.3390\/insects16050513","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T12:18:06Z","timestamp":1747052286000},"page":"513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Climate Change: A Major Factor in the Spread of Aedes aegypti (Diptera: Culicidae) and Its Associated Dengue Virus"],"prefix":"10.3390","volume":"16","author":[{"given":"Shahid","family":"Majeed","sequence":"first","affiliation":[{"name":"Department of Entomology, University of Agriculture, Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waseem","family":"Akram","sequence":"additional","affiliation":[{"name":"Department of Entomology, University of Agriculture, Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9771-5461","authenticated-orcid":false,"given":"Muhammad","family":"Sufyan","sequence":"additional","affiliation":[{"name":"Department of Entomology, University of Agriculture, Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-0490","authenticated-orcid":false,"given":"Asim","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Entomology, University of Agriculture, Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidra","family":"Riaz","sequence":"additional","affiliation":[{"name":"District Head Quarter (DHQ), Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6303-5986","authenticated-orcid":false,"given":"Shahla","family":"Faisal","sequence":"additional","affiliation":[{"name":"Department of Statistics and Center of Data Science, Government College University, Faisalabad 38040, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8360-9705","authenticated-orcid":false,"given":"Muhammad","family":"Binyameen","sequence":"additional","affiliation":[{"name":"Department of Entomology, Faculty of Agricultural Sciences & Technology, Bahauddin Zakariya University, Multan 60800, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad I.","family":"Bashir","sequence":"additional","affiliation":[{"name":"Epidemic Prevention and Control Program, Directorate General Health Services Punjab, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahzad","family":"Hassan","sequence":"additional","affiliation":[{"name":"Epidemic Prevention and Control Program, Directorate General Health Services Punjab, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7317-0084","authenticated-orcid":false,"given":"Saba","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, The Women University Multan, Multan 66000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0057-6628","authenticated-orcid":false,"given":"Oksana","family":"Kucher","sequence":"additional","affiliation":[{"name":"College of Naturopathic Medicine, East Grinstead, West Sussex RH19 4LZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena A.","family":"Piven","sequence":"additional","affiliation":[{"name":"Public Health, Health and Hygiene, RUDN University, 6 Miklukho-Maklaya St., 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9816-7759","authenticated-orcid":false,"given":"Olga D.","family":"Kucher","sequence":"additional","affiliation":[{"name":"Department of Environmental Management, Institute of Environmental Engineering, RUDN University, 6 Miklukho-Maklaya St., 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.rser.2011.09.009","article-title":"A review of climate change, mitigation and adaptation","volume":"16","author":"VijayaVenkataRaman","year":"2012","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_2","first-page":"Si-S332","article-title":"State of the Climate in 2017","volume":"99","author":"Blunden","year":"2018","journal-title":"Bull. Am. Meteorol. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1080\/00139157.2025.2434494","article-title":"Global warming has accelerated: Are the United Nations and the public well-informed?","volume":"67","author":"Hansen","year":"2025","journal-title":"Environ. Sci. Policy Sustain. Dev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/416389a","article-title":"Ecological responses to recent climate change","volume":"416","author":"Walther","year":"2002","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s10393-010-0354-6","article-title":"Climate change, vector-borne disease and interdisciplinary research: Social science perspectives on an environment and health controversy","volume":"7","author":"Brisbois","year":"2010","journal-title":"EcoHealth"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1086\/650284","article-title":"Climate change and highland malaria: Fresh air for a hot debate","volume":"85","author":"Chaves","year":"2010","journal-title":"Q. Rev. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1128\/CMR.11.3.480","article-title":"Dengue and dengue hemorrhagic fever","volume":"11","author":"Gubler","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1111\/ele.13675","article-title":"The influence of vector-borne disease on human history: Socio-ecological mechanisms","volume":"24","author":"Athni","year":"2021","journal-title":"Ecol. Lett."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Onen, H., Luzala, M.M., Kigozi, S., Sikumbili, R.M., Muanga, C.K., Zola, E.N., Wendji, S.N., Buya, A.B., Balciunaitiene, A., and Vi\u0161kelis, J. (2023). Mosquito-borne diseases and their control strategies: An overview focused on green synthesized plant-based metallic nanoparticles. Insects, 14.","DOI":"10.3390\/insects14030221"},{"key":"ref_10","first-page":"225","article-title":"Impact of climate change on insect pests, pathogens and nematodes","volume":"19","author":"Reddy","year":"2013","journal-title":"Pest Manag. Hortic. Ecosyst."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"772","DOI":"10.4269\/ajtmh.2006.74.772","article-title":"Effects of microclimatic changes caused by deforestation on the survivorship and reproductive fitness of Anopheles gambiae in western Kenya highlands","volume":"74","author":"Afrane","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1242\/jeb.037564","article-title":"Challenges in predicting climate and environmental effects on vector-borne disease episystems in a changing world","volume":"213","author":"Tabachnick","year":"2010","journal-title":"J. Exp. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Shapiro, L.L.M., Whitehead, S.A., and Thomas, M.B. (2017). Quantifying the effects of temperature on mosquito and parasite traits that determine the transmission potential of human malaria. PLoS Biol., 15.","DOI":"10.1371\/journal.pbio.2003489"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s13071-019-3391-1","article-title":"The role of temperature in affecting carry-over effects and larval competition in the globally invasive mosquito Aedes albopictus","volume":"12","author":"Ezeakacha","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s12940-017-0326-1","article-title":"Potential impact of climate change on emerging vector-borne and other infections in the UK","volume":"16","author":"Baylis","year":"2017","journal-title":"Environ. Health"},{"key":"ref_16","first-page":"141","article-title":"Climate change and mosquito-borne disease","volume":"109","author":"Reiter","year":"2001","journal-title":"Environ. Health Perspect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/S0140-6736(02)09964-6","article-title":"Potential effect of population and climate changes on global distribution of dengue fever: An empirical model","volume":"360","author":"Hales","year":"2002","journal-title":"Lancet"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1590\/S0085-56262010000300021","article-title":"Impact of small variations in temperature and humidity on the reproductive activity and survival of Aedes aegypti (Diptera, Culicidae)","volume":"54","year":"2010","journal-title":"Rev. Bras. Entomol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/0035-9203(95)90412-3","article-title":"Dengue haemorrhagic fever outbreak in Karachi, Pakistan, 1994","volume":"89","author":"Chan","year":"1995","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_20","first-page":"306","article-title":"Endemic dengue fever: A seldom recognized hazard for Pakistani children","volume":"3","author":"Siddiqui","year":"2009","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_21","first-page":"553","article-title":"Dengue fever in patients admitted in tertiary care hospitals in Pakistan","volume":"64","author":"Munir","year":"2014","journal-title":"J. Pak. Med. Assoc."},{"key":"ref_22","first-page":"81","article-title":"Dengue in Pakistan: Journey from a disease-free to a hyper-endemic nation","volume":"5","author":"Khanani","year":"2011","journal-title":"J. Dow Univ. Health Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1093\/cid\/cis932","article-title":"Forecasting high-priority infectious disease surveillance regions: A socioeconomic model","volume":"56","author":"Chan","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/S1473-3099(15)70091-5","article-title":"Effect of climate change on vector-borne disease risk in the UK","volume":"15","author":"Medlock","year":"2015","journal-title":"Lancet Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S1473-3099(09)70104-5","article-title":"Climate change and infectious diseases in Europe","volume":"9","author":"Semenza","year":"2009","journal-title":"Lancet Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1007\/s42690-024-01241-5","article-title":"Efficacy of different blood meals for the mass rearing of Aedes aegypti (Diptera: Culicidae) population","volume":"44","author":"Majeed","year":"2024","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Couret, J., Dotson, E., and Benedict, M.Q. (2014). Temperature, larval diet, and density effects on development rate and survival of Aedes aegypti (Diptera: Culicidae). PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087468"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e18","DOI":"10.1590\/s1678-9946201961018","article-title":"Abiotic factors and population dynamic of Aedes aegypti and Aedes albopictus in an endemic area of dengue in Brazil","volume":"61","author":"Custodio","year":"2019","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1007\/s42690-022-00742-5","article-title":"Influence of abiotic factors on the oviposition of Aedes (Stegomyia) aegypti (Diptera: Culicidae) in Northern Paran\u00e1, Brazil","volume":"42","author":"Pescim","year":"2022","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e200046","DOI":"10.1590\/0074-02760200046","article-title":"The influence of meteorological variables on the oviposition dynamics of Aedes aegypti (Diptera: Culicidae) in four environmentally distinct areas in northeast Brazil","volume":"115","author":"Santos","year":"2020","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/jvec.12244","article-title":"The effect of inbreeding and larval feeding regime on immature development of Aedes albopictus","volume":"42","author":"Jong","year":"2017","journal-title":"J. Vector Ecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1111\/j.0269-283X.2004.00495.x","article-title":"Temperature-related duration of aquatic stages of the Afrotropical malaria vector mosquito Anopheles gambiae in the laboratory","volume":"18","author":"Bayoh","year":"2004","journal-title":"Med. Vet. Entomol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.actatropica.2011.04.004","article-title":"Effects of different temperature regimens on the development of Aedes aegypti (L.) (Diptera: Culicidae) mosquitoes","volume":"119","author":"Mohammed","year":"2011","journal-title":"Acta Trop."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/jvec.12358","article-title":"The effects of temperature and shading on mortality and development rates of Aedes aegypti (Diptera: Culicidae)","volume":"44","author":"Sukiato","year":"2019","journal-title":"J. Vector Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0022-1910(99)00085-2","article-title":"Adaptation of Aedes aegypti (Diptera: Culicidae) oviposition behavior in response to humidity and diet","volume":"45","author":"Canyon","year":"1999","journal-title":"J. Insect Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1603\/ME13214","article-title":"The impact of temperature on the bionomics of Aedes (Stegomyia) aegypti, with special reference to the cool geographic range margins","volume":"51","author":"Eisen","year":"2014","journal-title":"J. Med. Entomol."},{"key":"ref_37","first-page":"217","article-title":"Influence of some environmental factors on the viability and hatching of Aedes aegypti (L.) eggs","volume":"28","author":"Mulla","year":"1968","journal-title":"Mosq. News."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"104726","DOI":"10.1016\/j.jinsphys.2024.104726","article-title":"Effects of temperature and humidity on the survival and hatching response of diapausing and non-diapausing Aedes aegypti eggs","volume":"161","author":"Fischer","year":"2025","journal-title":"J. Insect Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Carrington, L.B., Armijos, M.V., Lambrechts, L., and Scott, T.W. (2013). Fluctuations at a low mean temperature accelerate dengue virus transmission by Aedes aegypti. PLoS Neglected Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002190"},{"key":"ref_40","first-page":"217","article-title":"Studies on the behaviour of Anopheles minimus","volume":"4","author":"Thomson","year":"1941","journal-title":"J. Malar. Inst. India"},{"key":"ref_41","unstructured":"Dickerson, C.Z. (2007). The Effects of Temperature and Humidity on the Eggs of Aedes aegypti (L.) and Aedes albopictus (Skuse) in Texas. [Ph.D. Thesis, Texas A&M University]."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Estallo, E.L., Ludue\u00f1a-Almeida, F.F., Introini, M.V., Zaidenberg, M., and Almir\u00f3n, W.R. (2015). Weather variability associated with Aedes (Stegomyia) aegypti (Dengue vector) oviposition dynamics in Northwestern Argentina. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0127820"},{"key":"ref_43","first-page":"509","article-title":"Laboratory oviposition, fecundity and egg hatching ability of colonized Anopheles albimanus from southwestern Mexico","volume":"4","author":"Ramsey","year":"1988","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1504\/IJGW.2020.108671","article-title":"The effect of temperature on the development of immature stages of Aedes spp. against breeding containers","volume":"21","author":"Awang","year":"2020","journal-title":"Int. J. Glob. Warm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/22221751.2019.1707125","article-title":"Increased temperatures reduce the vectorial capacity of Aedes mosquitoes for Zika virus","volume":"9","author":"Onyango","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1186\/1756-3305-5-225","article-title":"Diet and density dependent competition affect larval performance and oviposition site selection in the mosquito species Aedes albopictus (Diptera: Culicidae)","volume":"5","author":"Yoshioka","year":"2012","journal-title":"Parasites Vectors"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zapletal, J., Erraguntla, M., Adelman, Z.N., Myles, K.M., and Lawley, M.A. (2018). Impacts of diurnal temperature and larval density on aquatic development of Aedes aegypti. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194025"},{"key":"ref_48","first-page":"223","article-title":"Effect of larval density on the development of Aedes aegypti (L.) and the size of adults","volume":"1","author":"Wada","year":"1965","journal-title":"Quaest. Entomol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1603\/ME13003","article-title":"The effect of temperature on life history traits of Culex mosquitoes","volume":"51","author":"Ciota","year":"2014","journal-title":"J. Med. Entomol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0033-3549(04)50218-2","article-title":"Climatic variables and transmission of malaria: A 12-year data analysis in Shuchen County, China","volume":"118","author":"Bi","year":"2003","journal-title":"Public Health Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"131","DOI":"10.4269\/ajtmh.1996.55.131","article-title":"Plasmodium falciparum malaria and climate change in the Northwest Frontier Province of Pakistan","volume":"55","author":"Bouma","year":"1996","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Chen, M.J., Lin, C.Y., Wu, Y.T., Wu, P.C., Lung, S.C., and Su, H.J. (2012). Effects of extreme precipitation on the distribution of infectious diseases in Taiwan, 1994\u20132008. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0034651"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Sirisena, P.D.N.N., Noordeen, F., Kurukulasuriya, H., Romesh, T.A., and Fernando, L. (2017). Effect of climatic factors and population density on the distribution of dengue in Sri Lanka: A GIS-based evaluation for prediction of outbreaks. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0166806"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"852","DOI":"10.26719\/emhj.19.019","article-title":"Role of climatic factors in the incidence of dengue in Port Sudan City, Sudan","volume":"25","author":"Noureldin","year":"2019","journal-title":"East. Mediterr. Health J."},{"key":"ref_55","first-page":"8","article-title":"Study of potential risk of dengue disease outbreak in Sri Lanka using GIS and statistical modelling","volume":"8","author":"Pathirana","year":"2009","journal-title":"J. Rural Trop. Public Health"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1080\/09603123.2011.572279","article-title":"The influence of climate variables on dengue in Singapore","volume":"21","author":"Pinto","year":"2011","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e2016052","DOI":"10.4178\/epih.e2016052","article-title":"Empirical model for estimating dengue incidence using temperature, rainfall, and relative humidity: A 19-year retrospective analysis in East Delhi","volume":"38","author":"Ramachandran","year":"2016","journal-title":"Epidemiol. Health"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Ahmad, R., Suzilah, I., Wan Najdah, W.M.A., Topek, O., Mustafakamal, I., and Lee, H.L. (2018). Factors determining dengue outbreak in Malaysia. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193326"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Somboonsak, P. (2019, January 21\u201323). Forecasting dengue fever epidemics using ARIMA model. Proceedings of the 2019 2nd Artificial Intelligence and Cloud Computing Conference, Kobe, Japan.","DOI":"10.1145\/3375959.3375970"},{"key":"ref_60","first-page":"299","article-title":"Epidemiology of dengue: Past, present and future prospects","volume":"5","author":"Murray","year":"2013","journal-title":"Clin. Epidemiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4537","DOI":"10.3390\/ijerph9124537","article-title":"North American wetlands and mosquito control","volume":"9","author":"Rey","year":"2012","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.ijid.2013.10.012","article-title":"Evolution of dengue in Sri Lanka\u2014Changes in the virus, vector, and climate","volume":"19","author":"Sirisena","year":"2014","journal-title":"Int. J. Infect. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1046\/j.1365-2915.2000.00207.x","article-title":"Effects of temperature and larval diet on development rates and survival of the dengue vector Aedes aegypti in north Queensland, Australia","volume":"14","author":"Burkot","year":"2000","journal-title":"Med. Vet. Entomol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.envres.2016.07.026","article-title":"Dengue in a changing climate","volume":"151","author":"Ebi","year":"2016","journal-title":"Environ. Res."},{"key":"ref_65","first-page":"942","article-title":"The effect of extrinsic incubation temperature on development of dengue serotype 2 and 4 viruses in Aedes aegypti (L.)","volume":"40","author":"Rohani","year":"2009","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2224","DOI":"10.1016\/j.scitotenv.2008.11.034","article-title":"Higher temperature and urbanization affect the spatial patterns of dengue fever transmission in subtropical Taiwan","volume":"407","author":"Wu","year":"2009","journal-title":"Sci. Total Environ."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/5\/513\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:30:53Z","timestamp":1760031053000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/5\/513"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,11]]},"references-count":66,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["insects16050513"],"URL":"https:\/\/doi.org\/10.3390\/insects16050513","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,11]]}}}