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Despite the widespread application of the CRISPR\/Cas9 gene-editing system in several insect orders, its utilization in locusts, particularly in the desert locust, has remained relatively unexplored. We established a CRISPR\/Cas9-mediated gene-editing workflow for the desert locust using gene encoding for neuropeptide corazonin (Crz) as a target. We also analyzed the phenotypic and physiological characteristics of the mutant using paraffin sectioning, HE staining, and chitin staining techniques. Our findings revealed that while Crz knockout desert locusts were viable and maintained normal fertility, they exhibited striking phenotypic alterations, including albinism and a significant reduction in cuticle thickness. These observations not only highlight the functional role of Crz in pigmentation and cuticle development but also underscore the potential of CRISPR\/Cas9 as a powerful tool for dissecting gene function in locusts. Furthermore, the successful application of CRISPR\/Cas9 in desert locusts also paves the way for similar genetic studies in other non-model insects, expanding the scope of functional genomics in entomology.<\/jats:p>","DOI":"10.3390\/insects16070704","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T13:44:19Z","timestamp":1752241459000},"page":"704","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["CRISPR\/Cas9-Mediated Knockout of the Corazonin Gene Indicates Its Regulation on the Cuticle Development of Desert Locusts (Schistocerca gregaria)"],"prefix":"10.3390","volume":"16","author":[{"given":"Yingying","family":"He","sequence":"first","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"},{"name":"School of Life Science, Shanxi University, Taiyuan 030006, China"},{"name":"Institute of Zoology-Developmental Biology, University of Cologne, 50923 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7858-1877","authenticated-orcid":false,"given":"Qiang","family":"Yan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Life Sciences, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Bi","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"},{"name":"School of Life Science, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guosheng","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Life Sciences, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, School of Life Sciences, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2237-3417","authenticated-orcid":false,"given":"Xiaoming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Warren","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Keele University, Huxley Building, Newcastle ST5 5BG, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siegfried","family":"Roth","sequence":"additional","affiliation":[{"name":"School of Life Science, Shanxi University, Taiyuan 030006, China"},{"name":"Institute of Zoology-Developmental Biology, University of Cologne, 50923 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3894-837X","authenticated-orcid":false,"given":"Tingting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"},{"name":"Institute of Zoology-Developmental Biology, University of Cologne, 50923 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kimathi, E., Tonnang, H.E.Z., Subramanian, S., Cressman, K., Abdel-Rahman, E.M., Tesfayohannes, M., Niassy, S., Torto, B., Dubois, T., and Tanga, C.M. 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