{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T20:43:09Z","timestamp":1779223389989,"version":"3.51.4"},"reference-count":65,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32272608"],"award-info":[{"award-number":["32272608"]}]},{"name":"National Natural Science Foundation of China","award":["32460668"],"award-info":[{"award-number":["32460668"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The high reproductive performance of Nilaparvata lugens (St\u00e5l) (Hemiptera: Delphacidae) is one of the key factors contributing to its serious damage. Studies have demonstrated that trehalose hydrolysis, catalyzed by trehalase (TRE), plays an important role in the reproductive regulation of N. lugens. However, it remains unclear how TRE regulates the reproduction of N. lugens. To address this question, the current study was designed to investigate the effects of TRE on the reproductive regulatory network of N. lugens. Specifically, RNA interference (RNAi) was conducted by injecting double-stranded RNA (dsRNA) targeting the TRE genes, and a trehalase inhibitor, validamycin, was used to suppress TRE activity. Subsequently, the relative expression levels of key genes involved in the reproductive regulatory pathway, as well as the triglyceride content in the fat body and ovary, were determined. The results indicated that both dsTREs and validamycin treatment decreased the relative expression of Vitellogenin (Vg) without affecting the expression of its receptor, VgR. However, their impact on the reproductive regulatory network differed: dsTREs injection did not affect the relative expression of JHAMT and Met in the juvenile hormone signaling pathway, but resulted in the upregulation of USP and TOR, and inhibition of InR2 and S6K. In contrast, validamycin injection inhibited the expression of Met, USP, EcR, InR1, TOR, and S6K. Both dsTREs and validamycin inhibited the expression of Fas, but only validamycin decreased the ovarian triglyceride content. These findings suggest that the inhibition of TRE leads to alterations in reproductive regulatory signals, thereby reducing the reproductive capacity of N. lugens.<\/jats:p>","DOI":"10.3390\/insects16070725","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T15:48:22Z","timestamp":1752680902000},"page":"725","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of Trehalase on the Gene Expression of the Reproductive Regulation Pathway Network and Triglyceride Metabolism in Nilaparvata lugens (St\u00e5l)"],"prefix":"10.3390","volume":"16","author":[{"given":"Bin","family":"Tang","sequence":"first","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxin","family":"Ge","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongkang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwen","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Han","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1000-5687","authenticated-orcid":false,"given":"Gao","family":"Hu","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guiyang University, Guiyang 550005, China"},{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wu","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, Guiyang University, Guiyang 550005, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Xu, H.J., and Zhang, C.X. (2017). Insulin receptors and wing dimorphism in rice planthoppers. Philos. Trans. R. Soc. B Biol. Sci., 372.","DOI":"10.1098\/rstb.2015.0489"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lin, X.M., Zhang, H.H., Gao, H.L., Yuan, X.W., and Liu, Z.W. (2023). The transcription factor CREB3-2 regulated neutral lipase gene expression in ovary of Nilaparvata lugens. Pestic. Biochem. Physiol., 196.","DOI":"10.1016\/j.pestbp.2023.105632"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zhang, H.H., Yang, B.J., Xu, X.X., Zou, J.Z., and Liu, Z.W. (2024). Roles of spermary-specific carboxylesterases in Nilaparvata lugens reproduction: Opposite between insecticide-induced upregulation and resistance-associated overexpression. Int. J. Biol. Macromol., 290.","DOI":"10.1016\/j.ijbiomac.2024.138991"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1127\/entomologia\/2024\/2351","article-title":"Jasmonic acid changes associated with long-term control of lepidopteran rice pests after insecticide seed treatment","volume":"44","author":"Xia","year":"2024","journal-title":"Entomol. Gen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15403","DOI":"10.1021\/acs.jafc.0c05763","article-title":"Contribution of glutathione s-transferases to imidacloprid resistance in Nilaparvata lugens","volume":"68","author":"Yang","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Tang, B., Xu, K., Liu, Y., Zhou, Z., Karthi, S., Yang, H., and Li, C. (2022). A review of physiological resistance to insecticide stress in Nilaparvata lugens. 3 Biotech, 12.","DOI":"10.1007\/s13205-022-03137-y"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1002\/ps.6666","article-title":"Overexpression of ATP-binding cassette transporter Mdr49-like confers resistance to imidacloprid in the field populations of brown planthopper, Nilaparvata lugens","volume":"78","author":"Wang","year":"2022","journal-title":"Pest Manag. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.jinsphys.2011.03.012","article-title":"RNA interference unveils functions of the hypertrehalosemic hormone on cyclic fluctuation of hemolymph trehalose and oviposition in the virgin female Blattella germanica","volume":"57","author":"Huang","year":"2011","journal-title":"J. Insect Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8423","DOI":"10.1021\/acs.jafc.3c08898","article-title":"Impact of Three Thiazolidinone Compounds with Piperine Skeletons on Trehalase Activity and Development of Spodoptera frugiperda Larvae","volume":"72","author":"Tang","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1127\/entomologia\/2023\/2277","article-title":"Responses of aphid and ladybird to lead transfer through soil and broad beans","volume":"44","author":"Li","year":"2024","journal-title":"Entomol. Gen."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Avonce, N., Mendoza-Vargas, A., Morett, E., Morett, E., and Iturriaga, G. (2006). Insights on the evolution of trehalose biosynthesis. BMC Evol. Biol., 6.","DOI":"10.1186\/1471-2148-6-109"},{"key":"ref_12","first-page":"501","article-title":"Membrane-penetrating trehalase from Bombyx mori","volume":"14","author":"Mitsumasu","year":"2005","journal-title":"Molecular cloning and localization in larval midgut. Insect Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.1271\/bbb.70239","article-title":"Molecular cloning of cDNA for trehalase from the European honeybee, Apis mellifera L., and its heterologous expression in Pichia pastoris","volume":"71","author":"Lee","year":"2007","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Tang, B., Wei, P., Zhao, L.N., Shi, Z., Shen, Q., Yang, M., Xie, G., and Wang, S. (2016). Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathways in Tribolium castaneum. BMC Biotechnol., 16.","DOI":"10.1186\/s12896-016-0297-2"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zhao, L.N., Yang, M.M., Shen, Q.D., Liu, X.J., Shi, Z.K., Wang, S.G., and Tang, B. (2016). Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference. Sci. Rep., 6.","DOI":"10.1038\/srep27841"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.pestbp.2016.10.003","article-title":"Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens","volume":"137","author":"Tang","year":"2017","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Tang, B., Han, Y., Mao, Q.X., Fu, H.Y., Luo, Y.J., Hua, L.Y.H., Liu, B.S., Hu, G., Wang, S.G., and Desneux, N. (2024). Regulation of three novel pepper thiothiazolidinones on the fecundity of Spodoptera frugiperda. Pestic. Biochem. Physiol., 204.","DOI":"10.1016\/j.pestbp.2024.106033"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Matassini, C., Parmeggiani, C., and Cardona, F. (2020). New Frontiers on human safe insecticides and fungicides: An opinion on trehalase inhibitors. Molecules, 25.","DOI":"10.3390\/molecules25133013"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1111\/1744-7917.12963","article-title":"Validamycin treatment significantly inhibits the glycometabolism and chitin synthesis in the common cutworm, Spodoptera litura","volume":"29","author":"Yu","year":"2022","journal-title":"Insect Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/jat.4547","article-title":"In vivo evaluation of the neurogenotoxic effects of exposure to validamycin A in neuroblasts of Drosophila melanogaster larval brain","volume":"44","author":"Demir","year":"2024","journal-title":"J. Appl. Toxicol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1603\/EC14051","article-title":"Trehalase activity in fungus-growing termite, Odontotermes feae (Isoptera: Termitideae) and inhibitory effect of validamycin","volume":"107","author":"Tatun","year":"2014","journal-title":"J. Econ. Entomol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1093\/aesa\/sav111","article-title":"Developmental and Lethal Effects of Trehalase Inhibitor (Validamycin) on the Tribolium castaneum (Coleoptera: Tenebrionidae)","volume":"109","author":"Tatun","year":"2016","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_23","first-page":"311","article-title":"Suppression of Trehalase Activity by Validamycin Induces Mortality and Developmental Delays in Sitophilus zeamais (Coleoptera: Curculionidae)","volume":"59","author":"Wangsantitham","year":"2024","journal-title":"J. Entomol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1093\/jme\/tjaa004","article-title":"Validamycin A Delays Development and Prevents Flight in Aedes aegypti (Diptera: Culicidae)","volume":"57","author":"Marten","year":"2020","journal-title":"J. Med. Entomol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Shi, S., Zuo, H., Gao, L., Yi, X., and Zhong, G. (2018). Silencing of rieske iron-sulfur protein impacts upon the development and reproduction of Spodoptera exigua by regulating ATP synthesis. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.00575"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/j.jinsphys.2008.08.007","article-title":"Molecular characteristics of insect vitellogenins","volume":"54","author":"Tufail","year":"2008","journal-title":"J. Insect Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Shen, Y., Chen, Y.Z., Lou, Y.H., and Zhang, C.X. (2019). Vitellogenin and vitellogenin-like genes in the brown planthopper. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.01181"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wu, Z.X., Yang, L.B., He, Q.J., and Zhou, S.T. (2021). Regulatory mechanisms of vitellogenesis in insects. Front. Cell Dev. Biol., 8.","DOI":"10.3389\/fcell.2020.593613"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"E2173","DOI":"10.1073\/pnas.1305293110","article-title":"Juvenile hormone and its receptor, methoprene-tolerant, control the dynamics of mosquito gene expression","volume":"110","author":"Zou","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Hansen, I.A., Attardo, G.M., Rodriguez, S.D., and Drake, L.L. (2014). Four-way regulation of mosquito yolk protein precursor genes by juvenile hormone-, ecdysone-, nutrient-, and insulin-like peptide signaling pathways. Front. Physiol., 5.","DOI":"10.3389\/fphys.2014.00103"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1146\/annurev-ento-020117-043258","article-title":"Regulatory pathways controlling female insect reproduction","volume":"63","author":"Roy","year":"2018","journal-title":"Annu. Rev. Entomol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ibmb.2018.09.002","article-title":"Juvenile hormone promotes locust fat body cell polyploidization and vitellogenesis by activating the transcription of Cdk6 and E2f1","volume":"102","author":"Wu","year":"2018","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cois.2018.07.010","article-title":"Juvenile hormone signaling in insect oogenesis","volume":"31","author":"Santos","year":"2019","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e2023470118","DOI":"10.1073\/pnas.2023470118","article-title":"Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito Aedes aegypti","volume":"118","author":"Ling","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e2104461118","DOI":"10.1073\/pnas.2104461118","article-title":"Juvenile hormone signaling promotes ovulation and maintains egg shape by inducing expression of extracellular matrix genes","volume":"118","author":"Luo","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Wu, M.Z., Fan, S.T., Zhang, Y.C., Tan, J.F., and Zhu, G.H. (2024). Disrupting shadow in the prothoracic gland induced larval development arrest in the fall armyworm Spodoptera frugiperda. Front. Physiol., 15.","DOI":"10.3389\/fphys.2024.1502753"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s13071-020-04558-5","article-title":"20-Hydroxyecdysone (20E) signaling as a promising target for the chemical control of malaria vectors","volume":"14","author":"Ekoka","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.ento.51.110104.151011","article-title":"Signaling and function of insulin-like peptides in insects","volume":"51","author":"Wu","year":"2006","journal-title":"Annu. Rev. Entomol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kannan, K., and Fridell, Y.W. (2013). Functional implications of Drosophila insulin-like peptides in metabolism, aging, and dietary restriction. Front. Physiol., 4.","DOI":"10.3389\/fphys.2013.00288"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s11427-019-1734-6","article-title":"FoxO directly regulates the expression of TOR\/S6K and vitellogenin to modulate the fecundity of the brown planthopper","volume":"64","author":"Dong","year":"2021","journal-title":"Sci. China Life Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1021\/acs.jafc.2c07433","article-title":"Insulin receptor substrate-1 (IRS1) regulates oogenesis and vitellogenesis in Propylea japonica by Mediating the FOXO transcription factor expression, independent of JH and 20E signaling pathways","volume":"71","author":"Huangfu","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ygcen.2016.11.004","article-title":"Insulin-like peptide DILP6 regulates juvenile hormone and dopamine metabolism in Drosophila females","volume":"243","author":"Rauschenbach","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.cois.2015.08.003","article-title":"Nutritional control of insect reproduction","volume":"11","author":"Smykal","year":"2015","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.exger.2010.11.036","article-title":"Role of TOR signaling in aging and related biological processes in Drosophila melanogaster","volume":"46","author":"Katewa","year":"2011","journal-title":"Exp. Gerontol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Lu, K., Chen, X., Liu, W.T., Zhang, X.Y., Chen, M.X., and Zhou, Q. (2016). Nutritional signaling regulates vitellogenin synthesis and egg development through juvenile hormone in Nilaparvata lugens (St\u00e5l). Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030269"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lu, K., Chen, X., Liu, W.T., and Zhou, Q. (2016). TOR pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in Nilaparvata lugens (St\u00e5l). Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17040438"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.gene.2017.10.011","article-title":"Insulin-like peptides and DNA\/tRNA methyltransferases are involved in the nutritional regulation of female reproduction in Nilaparvata lugens (St\u00e5l)","volume":"639","author":"Lu","year":"2018","journal-title":"Gene"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zhou, X., Ye, Y.Z., Ogihara, M.H., Takeshima, M., Fujinaga, D., Liu, C.W., Zhu, Z., Kataoka, H., and Bao, Y.Y. (2020). Functional analysis of ecdysteroid biosynthetic enzymes of the rice planthopper, Nilaparvata lugens. Insect Biochem. Mol. Biol., 123.","DOI":"10.1016\/j.ibmb.2020.103428"},{"key":"ref_49","first-page":"1","article-title":"Trehalase regulates ovarian maturation and egg hatchability of Nilaparvata lugens (St\u00e5l)","volume":"31","author":"Liu","year":"2025","journal-title":"J. Pest Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nature14286","article-title":"Two insulin receptors determine alternative wing morphs in planthoppers","volume":"519","author":"Xu","year":"2015","journal-title":"Nature"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.jprot.2015.09.022","article-title":"Jinggangmycin increases fecundity of the brown planthopper, Nilaparvata lugens (St\u00e5l) via fatty acid synthase gene expression","volume":"130","author":"Li","year":"2016","journal-title":"J. Proteom."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.cbpa.2018.02.008","article-title":"Lipophorin receptor regulates Nilaparvata lugens fecundity by promoting lipid accumulation and vitellogenin biosynthesis","volume":"219\u2013220","author":"Lu","year":"2018","journal-title":"Comp. Biochem. Physiol. Part A Mol. Integr. Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.jinsphys.2015.01.007","article-title":"Molecular characterization and RNA interference analysis of vitellogenin receptor from Nilaparvata lugens (St\u00e5l)","volume":"73","author":"Lu","year":"2015","journal-title":"J. Insect Physiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1093\/jisesa\/ieaa108","article-title":"The modulation of trehalose metabolism by 20-hydroxyecdysone in Antheraea pernyi (Lepidoptera: Saturniidae) during its diapause termination and post-termination period","volume":"20","author":"Li","year":"2020","journal-title":"J. Insect Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2425","DOI":"10.1093\/jee\/toae244","article-title":"Tendency to mate with short-winged partner of the brown planthopper Nilaparvata lugens (Hemiptera: Delphacidae)","volume":"117","author":"He","year":"2024","journal-title":"J. Econ. Entomol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Xue, W.H., Xu, N., Chen, S.J., Liu, X.Y., Zhang, J.L., and Xu, H.J. (2021). Neofunctionalization of a second insulin receptor gene in the wing-dimorphic planthopper, Nilaparvata lugens. PLoS Genet., 17.","DOI":"10.1371\/journal.pgen.1009653"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/imb.12636","article-title":"Molecular characterization of insulin-like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)","volume":"29","author":"Xue","year":"2020","journal-title":"Insect Mol. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.ibmb.2006.01.014","article-title":"Lipid uptake by insect oocytes","volume":"36","author":"Ziegler","year":"2006","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1007\/s00018-006-6016-8","article-title":"The life cycle of neutral lipids: Synthesis, storage and degradation","volume":"63","author":"Athenstaedt","year":"2006","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev-ento-112408-085356","article-title":"Insect fat body: Energy, metabolism, and regulation","volume":"55","author":"Arrese","year":"2010","journal-title":"Annu. Rev. Entomol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Wang, W., Yang, R.R., Peng, L.Y., Zhang, L., Yao, Y.L., and Bao, Y.Y. (2021). Proteolytic activity of the proteasome is required for female insect reproduction. Open Biol., 11.","DOI":"10.1098\/rsob.200251"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Wang, W., Ma, Y., Yang, R.R., Cheng, X., Huang, H.J., Zhang, C.X., and Bao, Y.Y. (2021). An MD-2-related lipid-recognition protein is required for insect reproduction and integument development. Open Biol., 11.","DOI":"10.1098\/rsob.210170"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Lu, K., Wang, Y., Chen, X., Zhang, X., Li, W., Cheng, Y., Li, Y., Zhou, J., You, K., and Song, Y. (2019). Adipokinetic hormone receptor mediates trehalose homeostasis to promote vitellogenin uptake by oocytes in Nilaparvata lugens. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.01904"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s12986-020-00443-1","article-title":"Trehalose itself plays a critical role on lipid metabolism: Trehalose increases jejunum cytoplasmic lipid droplets which negatively correlated with mesenteric adipocyte size in both HFD-fed trehalase KO and WT mice","volume":"17","author":"Arai","year":"2020","journal-title":"Nutr. Metab."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/7\/725\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:10:55Z","timestamp":1760033455000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/7\/725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,16]]},"references-count":65,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["insects16070725"],"URL":"https:\/\/doi.org\/10.3390\/insects16070725","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,16]]}}}