{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:26:53Z","timestamp":1792002413405,"version":"4.4.0"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cBiodiversidade de Insecta na Amaz\u00f4nia\u2014Rede BIA\u201d project","award":["#407.627\/2013-8"],"award-info":[{"award-number":["#407.627\/2013-8"]}]},{"name":"\u201cBiodiversidade de Insecta na Amaz\u00f4nia\u2014Rede BIA\u201d project","award":["#2001683"],"award-info":[{"award-number":["#2001683"]}]},{"name":"\u201cBiodiversidade de Insecta na Amaz\u00f4nia\u2014Rede BIA\u201d project","award":["#2323041"],"award-info":[{"award-number":["#2323041"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)-POSGRAD Fellowship","award":["#407.627\/2013-8"],"award-info":[{"award-number":["#407.627\/2013-8"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)-POSGRAD Fellowship","award":["#2001683"],"award-info":[{"award-number":["#2001683"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM)-POSGRAD Fellowship","award":["#2323041"],"award-info":[{"award-number":["#2323041"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM) and the Coorden\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior- Brasil (CAPES)","award":["#407.627\/2013-8"],"award-info":[{"award-number":["#407.627\/2013-8"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM) and the Coorden\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior- Brasil (CAPES)","award":["#2001683"],"award-info":[{"award-number":["#2001683"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas (FAPEAM) and the Coorden\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior- Brasil (CAPES)","award":["#2323041"],"award-info":[{"award-number":["#2323041"]}]},{"name":"USA National Science Foundation","award":["#407.627\/2013-8"],"award-info":[{"award-number":["#407.627\/2013-8"]}]},{"name":"USA National Science Foundation","award":["#2001683"],"award-info":[{"award-number":["#2001683"]}]},{"name":"USA National Science Foundation","award":["#2323041"],"award-info":[{"award-number":["#2323041"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Lampyrinae Lucidotini contains nearly a third of the world\u2019s Lampyridae. The lack of revisions and the overlap of diagnostic features across taxonomic levels have hindered identification and, therefore, further studies of Lucidotini taxa. The use of terminalia and genital traits in Lucidotini phylogenies has been fundamental to inform and update the genus-level delimitations in this group. One important open question is whether the presence of increased-length abdominal segment VIII (in relation to segment VII) in the closely related genera Scissicauda and Haplocauda is synapomorphic or homoplastic. In recent collecting efforts combined with studies of specimens deposited in different scientific collections, we found specimens hypothesized as four new firefly species from the Amazon basin that share the unique characteristics of the male abdomen of Haplocauda species. To test the hypothesis that these new species are monophyletic and sisters to Haplocauda species, and that the augmented segment VIII is synapomorphic to Scissicauda + Haplocauda, we ran phylogenetic analyses building upon a pre-existing character matrix, including a wide sample of Lucidotini and neighboring branches. Our results support the placement of the four new species described here (H. lata sp. nov., H. amazonensis sp. nov., H. aculeata sp. nov. and H. antimary sp. nov.) in Haplocauda. Importantly, one species with regular-sized segment VIII sclerites was recovered as the earliest diverging lineages of Haplocauda, suggesting that segment VIII was augmented at least twice in the Lucidotini\u2014the other one being within Scissicauda. We also report, for the first time, a sympatry between Haplocauda species. We revised the definition of Haplocauda and updated the distribution of H. mendesi and the existing key to species.<\/jats:p>","DOI":"10.3390\/insects16080824","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T13:20:09Z","timestamp":1754659209000},"page":"824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Four New Species of Haplocauda, with Notes on the Evolutionary Convergence of Copulation Clamps in Lucidotini (Coleoptera: Lampyridae: Lampyrinae)"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9321-7078","authenticated-orcid":false,"given":"Leandro","family":"Zeballos","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Sistem\u00e1tica e Ecologia de Coleoptera (LASEC), Coordena\u00e7\u00e3o de Biodiversidade (CBIO), Instituto Nacional de Pesquisas da Amaz\u00f4nia (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Felipe Lima da","family":"Silveira","sequence":"additional","affiliation":[{"name":"Biology Department, Western Carolina University, Cullowhee, NC 28723, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1955-288X","authenticated-orcid":false,"given":"Cl\u00e1udio Ruy Vasconcelos da","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Sistem\u00e1tica e Ecologia de Coleoptera (LASEC), Coordena\u00e7\u00e3o de Biodiversidade (CBIO), Instituto Nacional de Pesquisas da Amaz\u00f4nia (INPA), Manaus 69067-375, AM, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,8]]},"reference":[{"key":"ref_1","unstructured":"Keller, O. (2025, January 03). The Lampyridae of the World. Available online: https:\/\/lampyridae.world\/."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/isd\/ixz024","article-title":"Higher-Level Phylogeny and Reclassification of Lampyridae (Coleoptera: Elateroidea)","volume":"3","author":"Martin","year":"2019","journal-title":"Insect Syst. Divers."},{"key":"ref_3","first-page":"1","article-title":"The Taxonomy of the Lampyridae (Coleoptera)","volume":"90","author":"Mcdermott","year":"1964","journal-title":"Trans. Am. Entomol. Soc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3897\/asp.79.e67185","article-title":"Description and Phylogenetic Analysis of a New Firefly Genus from the Atlantic Rainforest, with Five New Species and New Combinations (Coleoptera: Lampyridae: Lampyrinae)","volume":"79","author":"Roza","year":"2021","journal-title":"Arthropod Syst. Phylogeny"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zeballos, L.F., Roza, A.S., Campello-Gon\u00e7alves, L., Vaz, S., Da Fonseca, C.R.V., Rivera, S.C., and da Silveira, L.F.L. (2023). Phylogeny of Scissicauda Species, with Eight New Species, Including the First Photinini Fireflies with Biflabellate Antennae (Coleoptera: Lampyridae). Diversity, 15.","DOI":"10.3390\/d15050620"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Viana, J.H., Roza, A.S., Vaz, S., Powell, G., and da Silveira, L.F.L. (2025). A New Firefly Genus from South America, with Seven New Species, a New Combination, and Notes on the Phylogeny of Lampyrinae: Lucidotini (Coleoptera: Lampyridae). PeerJ, 13.","DOI":"10.7717\/peerj.18967"},{"key":"ref_7","first-page":"55","article-title":"Systematic Review of the Firefly Genus Scissicauda (Coleoptera, Lampyridae, Amydetinae) from Brazil","volume":"2016","author":"Mermudes","year":"2016","journal-title":"Zookeys"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/zoolinnean\/zlaa041","article-title":"Systematic Review and Phylogeny of the Firefly Genus Dilychnia (Lampyridae: Lampyrinae), with Notes on Geographical Range","volume":"190","author":"Vaz","year":"2020","journal-title":"Zool. J. Linn. Soc."},{"key":"ref_9","first-page":"79","article-title":"Memoan Ciceroi Gen. et Sp. Nov., a Remarkable New Firefly Genus and Species from the Atlantic Rainforest (Coleoptera: Lampyridae)","volume":"3640","author":"Mermudes","year":"2013","journal-title":"Zootaxa"},{"key":"ref_10","first-page":"201","article-title":"Systematic Review of the Firefly Genus Amydetes Illiger, 1807 (Coleoptera: Lampyridae), with Description of 13 New Species","volume":"3765","author":"Mermudes","year":"2014","journal-title":"Zootaxa"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Roza, A.S., Mermudes, J.R.M., and Silveira, L.F.L.d. (2022). A New Genus and Two New Species of Fireflies from South America (Lampyridae: Lampyrinae: Photinini). Diversity, 14.","DOI":"10.3390\/d14111005"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Campello-Gon\u00e7alves, L., Roza, A.S., Rivera, S., Mermudes, J.R.M., Branham, M.A., and Lima da Silveira, L.F. (2025). Systematics of Amydetinae (Coleoptera: Lampyridae). Syst. Biodivers., 23.","DOI":"10.1080\/14772000.2025.2481663"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"da Silveira, L.F.L., Lima, W., da Fonseca, C.R.V., and McHugh, J. (2022). Haplocauda, a New Genus of Fireflies Endemic to the Amazon Rainforest (Coleoptera: Lampyridae). Insects, 13.","DOI":"10.3390\/insects13010058"},{"key":"ref_14","first-page":"421","article-title":"The Hind Wing of Coleoptera (Insecta): Morphology, Nomenclature and Phylogenetic Significance. Part 1. General Discussion and Archostemata-Elateroidea","volume":"71","author":"Lawrence","year":"2021","journal-title":"Annal Zool."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.11646\/zootaxa.3782.1.1","article-title":"Biogeographical Regionalisation of the Neotropical Region","volume":"3782","author":"Morrone","year":"2014","journal-title":"Zootaxa"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Morrone, J.J., Escalante, T., Rodr\u00edguez-Tarpia, G., Carmona, A., Arana, M., and Mercado-G\u00f3mez, J.D. (2022). Biogeographic Regionalization of the Neotropical Region: New Map and Shapefile. An. Acad. Bras. Cienc., 94.","DOI":"10.1590\/0001-3765202220211167"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"439","DOI":"10.32614\/RJ-2018-009","article-title":"Simple Features for R: Standardized Support for Spatial Vector Data","volume":"10","author":"Pebesma","year":"2018","journal-title":"R J."},{"key":"ref_18","unstructured":"Wickham, H., Fran\u00e7ois, R., Henry, L., M\u00fcller, K., and Vaughan, D. (2025, June 04). Dplyr: A Grammar of Data Manipulation. Available online: https:\/\/CRAN.R-project.org\/package=dplyr."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). Ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1111\/j.1096-0031.2007.00161.x","article-title":"Logical Basis for Morphological Characters in Phylogenetics","volume":"23","author":"Sereno","year":"2007","journal-title":"Cladistics"},{"key":"ref_21","unstructured":"Maddison, W.P., and Maddison, D.R. (2025, January 14). Mesquite: A Modular System for Evolutionary Analysis. Available online: http:\/\/www.mesquiteproject.org."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1096-0031.1994.tb00179.x","article-title":"BRANCH SUPPOLRT AND TREE STABILITY","volume":"10","author":"Bremer","year":"1994","journal-title":"Cladistics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1111\/j.1096-0031.2008.00209.x","article-title":"Weighting against Homoplasy Improves Phylogenetic Analysis of Morphological Data Sets","volume":"24","author":"Goloboff","year":"2008","journal-title":"Cladistics"},{"key":"ref_24","unstructured":"Nixon, K.C. (2021). WinClada, Version 2.0, Nixon, K.C."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3897\/zookeys.88.807","article-title":"Family-Group Names In Coleoptera (Insecta)","volume":"88","author":"Bouchard","year":"2011","journal-title":"Zookeys"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Fu, X., and Ballantyne, L. (2025). An Overview of the Firefly Genus Pygoluciola Wittmer, a Phylogeny of the Luciolinae Using Mitochondrial Genomes, a Description of Six New Species, and an Assessment of a Copulation Clamp in This Genus (Coleoptera: Lampyridae: Luciolinae). Insects, 16.","DOI":"10.3390\/insects16040394"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mayr, E. (1963). Animal Species and Evolution, Harvard University Press.","DOI":"10.4159\/harvard.9780674865327"},{"key":"ref_28","first-page":"273413","article-title":"Synergy between Allopatry and Ecology in Population Differentiation and Speciation","volume":"2012","author":"Johansson","year":"2012","journal-title":"Int. J. Ecol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1111\/zsc.12566","article-title":"Unlocking the Evolution of Abdominal Specializations in Luciuranus Fireflies (Coleoptera, Lampyridae)","volume":"51","author":"Silveira","year":"2022","journal-title":"Zool. Scr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev.ento.53.103106.093346","article-title":"Flash Signal Evolution, Mate Choice, and Predation in Fireflies","volume":"53","author":"Lewis","year":"2008","journal-title":"Annu. Rev. Entomol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/8\/824\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:26:36Z","timestamp":1760034396000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/16\/8\/824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,8]]},"references-count":30,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["insects16080824"],"URL":"https:\/\/doi.org\/10.3390\/insects16080824","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,8]]}}}