{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:42:53Z","timestamp":1760060573592,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Armed Forces Pest Management Board (AFPMB)","award":["#23-102"],"award-info":[{"award-number":["#23-102"]}]},{"name":"Uniformed Services University Graduate Student Research Award","award":["#23-102"],"award-info":[{"award-number":["#23-102"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Hydrogen peroxide (H2O2) occurs in the environment, including in aquatic environments where mosquitoes might lay eggs. However, little is known about the compound\u2019s impact on mosquitoes. We conducted an experiment to determine the effect of H2O2 on Ae. aegypti oviposition behavior and egg hatching using H2O2 concentrations similar to those in natural aquatic environments. Oviposition behavior was evaluated by dual-choice and multi-choice bioassays. Gravid Ae. aegypti mosquitoes were placed in cages with containers with different H2O2 concentrations (5, 25, 50, and 100 \u03bcM). After 72 h, the number of eggs laid was compared between oviposition sites with and without H2O2. Additionally, egg hatching was assessed under long-period exposure (48 h) and short-period exposure (2, 4, and 6 h and then in deionized water for up to 48 h). Results showed no significant difference in oviposition preference scores in the multi-choice assay (OAI = \u22120.135 \u00b1 0.06) (p = 0.138), but a significant difference in the dual-choice assay (0.195 \u00b1 0.01) (p = 0.001). Long-period exposure to H2O2 did not significantly affect hatch rates (11.34%) (p = 0.363), but short-period exposure significantly impacted hatch rates (17%) (p = 0.0001), with period of exposure alone playing a significant role (p &lt; 0.0044). Eggs exposed to 100 \u03bcM H2O2 for 2 h (p = 0.0070) and 4 h (p = 0.0036) had significantly higher hatch rates compared to the control. This study demonstrates that low concentrations of H2O2 can influence oviposition site characteristics and egg hatch rates. Combined with other environmental factors, H2O2 can shape the reproductive success of Ae. aegypti, offering potential strategies for mosquito control.<\/jats:p>","DOI":"10.3390\/insects16090928","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T09:18:57Z","timestamp":1756977537000},"page":"928","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Hydrogen Peroxide on Oviposition Site Preference and Egg Hatching of the Aedes aegypti (Linnaeus) Mosquito"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2712-3196","authenticated-orcid":false,"given":"Luka","family":"Ndungu","sequence":"first","affiliation":[{"name":"Division of Occupational and Environmental Health Sciences, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"Roberts","sequence":"additional","affiliation":[{"name":"Division of Global Public Health, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis","family":"Long","sequence":"additional","affiliation":[{"name":"Division of Global Public Health, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6284-4346","authenticated-orcid":false,"given":"Emilie","family":"Goguet","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"},{"name":"The Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., 6720A Rockledge Drive, Bethesda, MD 20817, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6537-3065","authenticated-orcid":false,"given":"Alex","family":"Stubner","sequence":"additional","affiliation":[{"name":"Division of Occupational and Environmental Health Sciences, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9965-8923","authenticated-orcid":false,"given":"Sean","family":"Beeman","sequence":"additional","affiliation":[{"name":"Division of Occupational and Environmental Health Sciences, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Lewandowski","sequence":"additional","affiliation":[{"name":"Division of Occupational and Environmental Health Sciences, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3583-3291","authenticated-orcid":false,"given":"Bernard","family":"Okech","sequence":"additional","affiliation":[{"name":"Graduate Programs, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"David, M.R., Dantas, E.S., Maciel-de-Freitas, R., Code\u00e7o, C.T., Prast, A.E., and Louren\u00e7o-de-Oliveira, R. 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