{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:44:35Z","timestamp":1760060675201,"version":"build-2065373602"},"reference-count":67,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"IDB RAS Government basic research program","award":["0088-2024-0017"],"award-info":[{"award-number":["0088-2024-0017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Gene duplications are considered to be the major evolutionary resource of novel functions. The gene family Esf2\/ABP1 is conserved in metazoan organisms from yeast to humans. Here we performed a search and characterization of Esf2\/ABP1 homologs in the Drosophila genus. Whereas in the majority of Drosophila species this gene family is represented by only a single gene, in the melanogaster and suzukii subgroups recurrent gene duplications arose, providing 47 homologous genes located on the X chromosome. To study the evolutionary history of duplicates, we performed phylogenetic, functional domain, and tissue-specific expression analyses. We revealed a male-specific and testis-biased transcription pattern of duplicated copies in Drosophila melanogaster and Drosophila sechellia compared to ubiquitous expression of the parental gene. The amplification of 21 repeated paralogs within the heterochromatic piRNA cluster resulted in the ovarian-specific transformation of these repeats into piRNAs in D. melanogaster. In three species of the suzukii subgroup, Esf2\/ABP1 genes evolved with domain diversification: in addition to RNA-binding ABT1-like domain preservation, all homologous proteins acquired expanded intrinsically disordered regions. By studying the duplicated copies of the Esf2\/ABP1 family in Drosophila, we offer insight into how novel gene functions emerge and are maintained, contributing to life\u2019s diversity and complexity.<\/jats:p>","DOI":"10.3390\/insects16090956","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T10:50:04Z","timestamp":1757587804000},"page":"956","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recurrent Duplication, Testis-Biased Expression, and Functional Diversification of Esf2\/ABT1 Family Genes in Drosophila"],"prefix":"10.3390","volume":"16","author":[{"given":"Elizaveta D.","family":"Davydova","sequence":"first","affiliation":[{"name":"Laboratory of Functional Genomics, Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Str., 119334 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5866-3574","authenticated-orcid":false,"given":"Alexei A.","family":"Kotov","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Genomics, Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Str., 119334 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina V.","family":"Chernizova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Neurobiology of Learning, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 5A Butlerov Str., 117485 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0624-0246","authenticated-orcid":false,"given":"Ekaterina Yu.","family":"Yakovleva","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Genomics, Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Str., 119334 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7422-4387","authenticated-orcid":false,"given":"Ludmila V.","family":"Olenina","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Genomics, Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Str., 119334 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/nrg1204","article-title":"The origin of new genes: Glimpses from the young and old","volume":"4","author":"Long","year":"2003","journal-title":"Nat. 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