{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:24:37Z","timestamp":1778563477737,"version":"3.51.4"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31972616"],"award-info":[{"award-number":["31972616"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013058","name":"Key Technologies R&D Program of Jiangsu Province","doi-asserted-by":"crossref","award":["BE2021346"],"award-info":[{"award-number":["BE2021346"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agriculture Research System of China of MOF and MARA","award":["CARS-18-ZJ0101"],"award-info":[{"award-number":["CARS-18-ZJ0101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Background: Tissue-specific long non-coding RNAs (lncRNAs) represent potential biomarkers. The testis-enriched lncRNA41584, previously identified as downregulated in male-sterile silkworm mutants (JMS, GMS), is associated with male sterility, but its functional mechanism remained unknown. Subcellular localization, dual-luciferase reporter assays, MTT, and flow cytometry were employed to examine lncRNA41584\u2013miR-3047-z\u2013BmCDK20 interactions. In vivo functional validation included lncRNA41584 knockdown and miR-3047-z overexpression in Bombyx mori. lncRNA41584 localizes predominantly to the cytoplasm and acts as a competing endogenous RNA (ceRNA) by sponging miR-3047-z, thereby upregulating the cyclin-dependent kinase BmCDK20. Perturbation of this axis impaired BmN cell proliferation, causing G1 phase arrest, and led to spermatocyst malformation, reduced fertilization rates, and increased unfertilized eggs. The lncRNA41584\u2013miR-3047-z\u2013BmCDK20 ceRNA network is essential for testicular cell cycle progression and spermatogenesis in silkworms, offering mechanistic insights into lepidopteran male sterility and potential targets for pest fertility regulation.<\/jats:p>","DOI":"10.3390\/insects16111120","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:15:14Z","timestamp":1762175714000},"page":"1120","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The lncRNA41584-miR3047-z-BmCDK20 ceRNA Regulatory Network Influences Reproductive Development in Male Silkworms (Bombyx mori)"],"prefix":"10.3390","volume":"16","author":[{"given":"Tianchen","family":"Huang","sequence":"first","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9048-056X","authenticated-orcid":false,"given":"Dongxu","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heying","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoling","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/BF02126341","article-title":"Physiological role of apyrene spermatozoa of Bombyx mori","volume":"43","author":"Osanai","year":"1987","journal-title":"Cell. 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