{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T07:09:46Z","timestamp":1773385786976,"version":"3.50.1"},"reference-count":47,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Lymantria dispar (L.) and Dendrolimus sibiricus Tschetv. are lepidopteran forest pest species, with a long history of outbreak dynamics. The recently isolated strain of Cypovirus\u2014Dendrolimus sibiricus cypovirus-1 (DsCPV-1) shows potential as a bioinsecticide against these and other lepidopteran species. Manduca sexta (L.) has been identified as a promising producer of DsCPV-1. Although M. sexta offers clear advantages as an alternative host for DsCPV-1 production, the DsCPV-1 isolate passaged through M. sexta (DsCPV-Ms) produces fewer polyhedra than the original isolate. Here, we evaluated the virulence, recovery of polyhedron formation, and replication of the DsCPV-Ms in L. dispar (alternative host) and D. sibiricus (original host) larvae to assess its suitability as a biocontrol agent in these hosts. Our results demonstrate that DsCPV-Ms causes significant mortality along with efficient polyhedra synthesis in D. sibiricus larvae. In contrast, DsCPV-Ms infection of L. dispar resulted in no significant mortality despite detectable viral replication and polyhedron formation. Polyhedron formation in L. dispar was significantly lower following infection with DsCPV-Ms than with the original isolate, despite confirmed replication of DsCPV-Ms. These findings indicate that DsCPV-Ms remains effective against D. sibiricus; however, further improvements are needed before it can be applied to L. dispar.<\/jats:p>","DOI":"10.3390\/insects16121217","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T09:34:37Z","timestamp":1764581677000},"page":"1217","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Impact of DsCPV-1 Passage Through Alternative Host Manduca sexta on Its Virulence, Productivity, and Transmission Potential in Lepidopteran Forest Pests"],"prefix":"10.3390","volume":"16","author":[{"given":"Anna O.","family":"Subbotina","sequence":"first","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Olympic Avenue 1, Sirius 354340, Russia"},{"name":"Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3180-5998","authenticated-orcid":false,"given":"Yuriy B.","family":"Akhanaev","sequence":"additional","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Olympic Avenue 1, Sirius 354340, Russia"},{"name":"Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1066-1867","authenticated-orcid":false,"given":"Elena L.","family":"Arzhanova","sequence":"additional","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Olympic Avenue 1, Sirius 354340, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina A.","family":"Belousova","sequence":"additional","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Olympic Avenue 1, Sirius 354340, Russia"},{"name":"Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey V.","family":"Kolosov","sequence":"additional","affiliation":[{"name":"FBRI State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, Koltsovo 630559, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris S.","family":"Kondrashov","sequence":"additional","affiliation":[{"name":"Department of Natural Science, Novosibirsk State University, Pirogova Str. 1, Novosibirsk 630090, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia S.","family":"Melnikova","sequence":"additional","affiliation":[{"name":"Department of Natural Science, Novosibirsk State University, Pirogova Str. 1, Novosibirsk 630090, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7061-3772","authenticated-orcid":false,"given":"Vyacheslav V.","family":"Martemyanov","sequence":"additional","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Olympic Avenue 1, Sirius 354340, Russia"},{"name":"Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"ref_1","unstructured":"Pogue, M. 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