{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T11:35:35Z","timestamp":1768304135869,"version":"3.49.0"},"reference-count":63,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2024YFC2607600"],"award-info":[{"award-number":["2024YFC2607600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2025YFC2609100"],"award-info":[{"award-number":["2025YFC2609100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tian-Shan Talent Program","award":["2022TSYCCX0084"],"award-info":[{"award-number":["2022TSYCCX0084"]}]},{"name":"Xi-Zang Key Research and Development Program","award":["XZ202401ZY0031"],"award-info":[{"award-number":["XZ202401ZY0031"]}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["XBZX-04"],"award-info":[{"award-number":["XBZX-04"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Tomato leafminer (Tuta absoluta) significantly affects tomato (Solanum lycopersicum) and eggplant (Solanum melongena) crops worldwide, with its feeding patterns being closely associated with its gut microbiota. We aimed to compare the cultivable gut bacteria of T. absoluta larvae fed on tomato and eggplant to investigate their role in host adaptation. Gut bacteria were cultivated on Luria\u2013Bertani broth, nutrient agar, and Brain Heart Infusion media under different temperature conditions, followed by morphology- and 16S rRNA-based identification. Notably, both feeding groups revealed distinct gut bacterial community structures. Tomato-fed larvae harbored bacteria spanning eight species, five genera, four families, and two phyla. In contrast, eggplant-fed larvae exhibited greater microbial diversity, encompassing 15 species, 10 genera, 9 families, and 3 phyla, including unique genera such as Pseudomonas and Pectobacterium, which was attributed to the host plant contribution. Enterococcus mundtii was the most dominant bacterium, and species such as Bacillus wiedmannii and Micrococcus luteus were most thermotolerant. Overall, these findings highlight the importance of multi-condition culture approaches for thoroughly characterizing insect gut microbiota and underscore the role of host plants in pest adaptability by modulating gut microbial communities, providing new insights for developing sustainable control strategies utilizing \u201cplant\u2013insect\u2013microorganism\u201d interactions.<\/jats:p>","DOI":"10.3390\/insects17010081","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T09:13:01Z","timestamp":1768209181000},"page":"81","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-Condition Cultivation Reveals the Host Plant-Dependent Gut Bacteria Diversity in Tomato Leafminer (Tuta absoluta) Larvae"],"prefix":"10.3390","volume":"17","author":[{"given":"Xiaoyu","family":"Fang","sequence":"first","affiliation":[{"name":"College of Life Science, Shanxi Normal University, Taiyuan 030031, China"},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoyi","family":"Wen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science, Shanxi Normal University, Taiyuan 030031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7759-0811","authenticated-orcid":false,"given":"Jianyang","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjun","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6014-3383","authenticated-orcid":false,"given":"Nianwan","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4275-8632","authenticated-orcid":false,"given":"Fanghao","family":"Wan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1015-1510","authenticated-orcid":false,"given":"Zhichuang","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanxue","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1127\/entomologia\/2023\/2026","article-title":"Genetic diversity and genetic differentiation pattern of Tuta absoluta across China","volume":"43","author":"Wang","year":"2023","journal-title":"Entomol. 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