{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T11:47:22Z","timestamp":1776167242993,"version":"3.50.1"},"reference-count":73,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Key-Area Research and Development Program of Guangdong","award":["2026B0202190004"],"award-info":[{"award-number":["2026B0202190004"]}]},{"name":"Innovation Team Project in Modern Agricultural Industrial Technology System of Guangdong","award":["2025CXTD21"],"award-info":[{"award-number":["2025CXTD21"]}]},{"name":"Guangdong Special Support Program-Leading Talents in Science and Technology Innovation","award":["2023TX07A017"],"award-info":[{"award-number":["2023TX07A017"]}]},{"name":"Science and Technology Talent and Platform Project of Yunnan","award":["202405AF140082"],"award-info":[{"award-number":["202405AF140082"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Bactrocera dorsalis (Hendel) is a major fruit-feeding pest that poses a severe and persistent threat to the horticulture industry in tropical and subtropical regions. Methyl eugenol (ME) is a powerful male-specific attractant phytochemical and pheromone precursor that has been widely exploited in lure-and-kill pest management programs. Upon ingestion, ME is metabolized (E)-coniferyl alcohol (E-CF) and 2-allyl-4,5-dimethoxyphenol (DMP), which are stored in the male rectal glands and released during courtship to attract females. Despite its ecological significance, the fundamental molecular mechanism underlying ME perception remains poorly understood. Here, we performed a comparative transcriptomic analysis of ME-responsive and ME-non-responsive male B. dorsalis across four tissues (head, gut, midleg, and wing). A total of 15,727 genes were annotated, of which 970 were associated with odorant-binding proteins (OBPs), odorant receptors (ORs), gustatory receptors (GRs), ionotropic receptors (IRs), and chemosensory proteins (CSPs), as well as detoxification families comprising cytochrome P450s (CYPs), carboxylesterases (CaEs), glutathione S-transferases (GSTs), and uridine diphosphate (UDP)-glycosyltransferases (UGTs), and the stress-related heat shock proteins (HSPs) genes. Differential expression analysis identified 7222, 7763, and 6105 differentially expressed genes (DEGs) in the head, gut, and wings\/midlegs, respectively, between ME-responsive and ME-non-responsive males. Notably, CYPs, UGTs, and HSPs involved in detoxification and stress response were significantly downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that CYPs were significantly enriched in metabolic detoxification pathways. These findings reveal a complex molecular interplay between olfaction and detoxification and suggest that ME induces coordinated genetic pathways supporting survival, reproduction, and environmental adaptability. This knowledge provides a foundation for the development of eco-friendly pest management strategies targeting these molecular mechanisms.<\/jats:p>","DOI":"10.3390\/insects17040416","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T10:29:08Z","timestamp":1776162548000},"page":"416","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Understanding the Chemosensory and Detoxification Mechanisms in the Oriental Fruit Fly, Bactrocera dorsalis"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7664-7869","authenticated-orcid":false,"given":"Saleem","family":"Jaffar","sequence":"first","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5813-8446","authenticated-orcid":false,"given":"Yongyue","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1038\/s41467-018-03384-9","article-title":"Strong phenotypic plasticity limits potential for evolutionary responses to climate change","volume":"9","author":"Oostra","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Anton, S., and Cortesero, A.-M. (2022). Plasticity in Chemical Host Plant Recognition in Herbivorous Insects and Its Implication for Pest Control. Biology, 11.","DOI":"10.3390\/biology11121842"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/phen.12428","article-title":"Olfactory receptors in Bactrocera species for sustainable fruit fly management: A review and future perspectives","volume":"49","author":"Jaffar","year":"2024","journal-title":"Physiol. Entomol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/s42003-021-02966-6","article-title":"Chromosome-level genome assembly of Bactrocera dorsalis reveals its adaptation and invasion mechanisms","volume":"5","author":"Jiang","year":"2022","journal-title":"Commun. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1038\/s42003-024-06505-x","article-title":"AANAT1 regulates insect midgut detoxification through the ROS\/CncC pathway","volume":"7","author":"Zeng","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Pavlidi, N., Dermauw, W., Rombauts, S., Chrysargyris, A., Chrisargiris, A., Van Leeuwen, T., and Vontas, J. (2013). Analysis of the Olive Fruit Fly Bactrocera oleae Transcriptome and Phylogenetic Classification of the Major Detoxification Gene Families. PLoS ONE, 8.","DOI":"10.1371\/annotation\/ab935a02-e8a2-4d7e-85cb-f8c48d6d9ba2"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"R519","DOI":"10.1016\/j.cub.2023.04.025","article-title":"Volatile-mediated plant\u2013plant communication and higher-level ecological dynamics","volume":"33","author":"Kessler","year":"2023","journal-title":"Curr. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.cbpc.2006.04.009","article-title":"Transport of methyl eugenol-derived sex pheromonal components in the male fruit fly, Bactrocera dorsalis","volume":"143","author":"Tan","year":"2006","journal-title":"Comp. Biochem. Physiol. Part C Toxicol. Pharmacol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Jaffar, S., Rizvi, S.A.H., and Lu, Y. (2023). Understanding the Invasion, Ecological Adaptations, and Management Strategies of Bactrocera dorsalis in China: A Review. Horticulturae, 9.","DOI":"10.3390\/horticulturae9091004"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev-ento-120811-153635","article-title":"Odorant Reception in Insects: Roles of Receptors, Binding Proteins, and Degrading Enzymes","volume":"58","author":"Leal","year":"2013","journal-title":"Annu. Rev. Entomol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1127\/entomologia\/3505","article-title":"Honeybees as sentinels of microplastic pollution: A meta-analysis of accumulation, toxicity, and biomonitoring potential","volume":"45","author":"Raza","year":"2025","journal-title":"Entomol. Gen."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S2095-3119(18)62015-5","article-title":"Invasion, expansion, and control of Bactrocera dorsalis (Hendel) in China","volume":"18","author":"Liu","year":"2019","journal-title":"J. Integr. Agric."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1127\/entomologia\/2024\/2711","article-title":"Advancements in Integrated Pest Management strategies for Bactrocera dorsalis in Asia: Current status, insights, and future prospects","volume":"44","author":"Wang","year":"2024","journal-title":"Entomol. Gen."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1146\/annurev-ento-012723-102658","article-title":"The global epidemic of Bactrocera pests: Mixed-species invasions and risk assessment","volume":"69","author":"Zhao","year":"2024","journal-title":"Annu. Rev. Entomol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Su, H., Zhao, J., Yu, H., Jaffar, S., Hao, Z., Liang, G., Zeng, L., and Lu, Y. (2025). Oviposition Competition Between Zeugodacus cucurbitae and Bactrocera dorsalis Adults in Five Hosts. Insects, 16.","DOI":"10.3390\/insects16040419"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Li, X., Li, P., Li, D., Cai, X., Gu, S., Zeng, L., Cheng, D., and Lu, Y. (2024). Dynamics of Bactrocera dorsalis resistance to seven insecticides in south China. Insects, 15.","DOI":"10.3390\/insects15090679"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Li, D., Chen, L., Cai, X., Qi, Y., and Lu, Y. (2024). Comparative Population Biology and Related Gene Expression in the Beta-Cypermethrin-Resistant Strains of Bactrocera dorsalis (Hendel). Insects, 15.","DOI":"10.3390\/insects15080569"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1007\/s10340-024-01789-8","article-title":"Evaluation of methyl eugenol-supplemented diet as pre-release treatment for simultaneous application of male annihilation and sterile insect techniques against Bactrocera dorsalis","volume":"98","author":"Wee","year":"2025","journal-title":"J. Pest Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"107015","DOI":"10.1016\/j.cropro.2024.107015","article-title":"Effects of semiochemical pre-feeding, physiological state, and weather on the response of Bactrocera dorsalis to methyl eugenol baited traps","volume":"188","author":"Pogue","year":"2025","journal-title":"Crop Prot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112567","DOI":"10.1016\/j.ecoenv.2021.112567","article-title":"Assessment of 2-allyl-4,5-dimethoxyphenol safety and attractiveness to mature males of Bactrocera dorsalis (Hendel)","volume":"223","author":"Deng","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"15004","DOI":"10.1038\/s41598-020-72209-x","article-title":"Male-lure type, lure dosage, and fly age at feeding all influence male mating success in Jarvis\u2019 fruit fly","volume":"10","author":"Wee","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1002\/ps.6713","article-title":"Assessment of attractancy and safeness of (E)-coniferyl alcohol for management of female adults of Oriental fruit fly, Bactrocera dorsalis (Hendel)","volume":"78","author":"Liu","year":"2022","journal-title":"Pest Manag. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Liu, H., Chen, Z.-S., Zhang, D.-J., and Lu, Y.-Y. (2018). BdorOR88a Modulates the Responsiveness to Methyl Eugenol in Mature Males of Bactrocera dorsalis (Hendel). Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.00987"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Banfi, D., Bianchi, T., Mastore, M., and Brivio, M.F. (2025). The Role of Heat Shock Proteins in Insect Stress Response, Immunity, and Climate Adaptation. Insects, 16.","DOI":"10.3390\/insects16070741"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101796","DOI":"10.1016\/j.jbc.2022.101796","article-title":"Mechanisms tailoring the expression of heat shock proteins to proteostasis challenges","volume":"298","author":"Alecki","year":"2022","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e18657","DOI":"10.1016\/j.heliyon.2023.e18657","article-title":"Identification of metabolizing enzyme genes associated with xenobiotics and odorants in the predatory stink bug Arma custos based on transcriptome analysis","volume":"9","author":"Li","year":"2023","journal-title":"Heliyon"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Volont\u00e9, M., Traverso, L., Sierra, I., Aptekmann, A.A., Nadra, A.D., and Ons, S. (2025). Characterization of odorant binding and chemosensory protein families in the kissing bug Triatoma infestans: Comparative analysis among Heteroptera species. BMC Genom., 26.","DOI":"10.1186\/s12864-025-11967-2"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7784","DOI":"10.1021\/acs.jafc.3c09791","article-title":"Odorant Receptor BdorOR49b Mediates Oviposition and Attraction Behavior of Bactrocera dorsalis to Benzothiazole","volume":"72","author":"Zhang","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5551","DOI":"10.1002\/ps.8908","article-title":"Multiple mechanosensory pathways mediate oviposition behavior in Bactrocera dorsalis (Diptera: Tephritidae)","volume":"81","author":"Zhang","year":"2025","journal-title":"Pest Manag. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Xu, L., Tang, K.Y., Chen, X.F., Tao, Y., Jiang, H.B., and Wang, J.J. (2021). Comparative transcriptomic analysis reveals female-biased olfactory genes potentially involved in plant volatile-mediated oviposition behavior of Bactrocera dorsalis. BMC Genom., 22.","DOI":"10.1186\/s12864-020-07325-z"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1111\/1744-7917.12997","article-title":"CRISPR-mediated mutagenesis of the odorant receptor co-receptor (Orco) gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis","volume":"29","author":"Xu","year":"2022","journal-title":"Insect Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"136774","DOI":"10.1016\/j.ijbiomac.2024.136774","article-title":"The gustatory receptor BdorGr43a mediated sucrose preference in the feeding of Bactrocera dorsalis","volume":"282","author":"Dong","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1021\/acs.jafc.0c05945","article-title":"OBP2 in the Midlegs of the Male Bactrocera dorsalis Is Involved in the Perception of the Female-Biased Sex Pheromone 4-Allyl-2,6-dimethoxyphenol","volume":"69","author":"Hu","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"110736","DOI":"10.1016\/j.ygeno.2023.110736","article-title":"Chromosome-level genome assembly of Bactrocera correcta provides insights into its adaptation and invasion mechanisms","volume":"115","author":"Guo","year":"2023","journal-title":"Genomics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8400","DOI":"10.1021\/acs.jafc.3c01563","article-title":"An Antenna-Abundant Glutathione S-Transferase BdGSTd8 Participates in Detoxification of Two Organophosphorus Insecticides in Bactrocera dorsalis (Hendel)","volume":"71","author":"Meng","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1673\/031.010.0801","article-title":"Pre-Release consumption of methyl eugenol increases the mating competitiveness of sterile males of the oriental fruit fly, Bactrocera dorsalis, in large field enclosures","volume":"10","author":"Shelly","year":"2010","journal-title":"J. Insect Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"nwae294","DOI":"10.1093\/nsr\/nwae294","article-title":"Methyl eugenol regulates mating behavior in oriental fruit flies by enhancing lek attractiveness","volume":"12","author":"Zhang","year":"2024","journal-title":"Natl. Sci. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1038\/s41467-018-03281-1","article-title":"The genomic and functional landscapes of developmental plasticity in the American cockroach","volume":"9","author":"Li","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"jkad133","DOI":"10.1093\/g3journal\/jkad133","article-title":"Evolution of chemosensory and detoxification gene families across herbivorous Drosophilidae","volume":"13","author":"Gloss","year":"2023","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.pestbp.2012.05.002","article-title":"Cuticular penetration of \u03b2-cypermethrin in insecticide-susceptible and resistant strains of Bactrocera dorsalis","volume":"103","author":"Lin","year":"2012","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1146\/annurev.ento.51.110104.151104","article-title":"Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics","volume":"52","author":"Li","year":"2007","journal-title":"Annu. Rev. Entomol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Abendroth, J.A., Moural, T.W., Wei, H., and Zhu, F. (2023). Roles of insect odorant binding proteins in communication and xenobiotic adaptation. Front. Insect Sci., 3.","DOI":"10.3389\/finsc.2023.1274197"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3951","DOI":"10.1021\/acs.jafc.4c10518","article-title":"Chemosensory Proteins Protect Nilaparvata lugens from Imidacloprid by Sequestering the Insecticide and Facilitating Metabolic Detoxification","volume":"73","author":"Deng","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"21012","DOI":"10.1073\/pnas.1909130116","article-title":"Overcoming insecticide resistance through computational inhibitor design","volume":"116","author":"Correy","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1017\/S0007485317001195","article-title":"Effects of insecticides chlorpyrifos, emamectin benzoate and fipronil on Spodoptera litura might be mediated by OBPs and CSPs","volume":"108","author":"Lin","year":"2018","journal-title":"Bull. Entomol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Sierra, I., Latorre-Estivalis, J.M., Traverso, L., Gonzalez, P.V., Aptekmann, A., Nadra, A.D., Masuh, H., and Ons, S. (2021). Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus. PLoS Neglected Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009587"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"20101","DOI":"10.1021\/acs.jafc.4c05026","article-title":"Overexpression of Two Odorant Binding Proteins Confers Chlorpyrifos Resistance in the Green Peach Aphid Myzus persicae","volume":"72","author":"Xiao","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Kefi, M., Charamis, J., Balabanidou, V., Ioannidis, P., Ranson, H., Ingham, V.A., and Vontas, J. (2021). Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs. BMC Genom., 22.","DOI":"10.1186\/s12864-021-08205-w"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/s41586-019-1864-1","article-title":"A sensory appendage protein protects malaria vectors from pyrethroids","volume":"577","author":"Ingham","year":"2020","journal-title":"Nature"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"106858","DOI":"10.1016\/j.ympev.2020.106858","article-title":"Analyses of chemosensory genes provide insight into the evolution of behavioral differences to phytochemicals in Bactrocera species","volume":"151","author":"Wu","year":"2020","journal-title":"Mol. Phylogenetics Evol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"163762","DOI":"10.1016\/j.scitotenv.2023.163762","article-title":"A sublethal dose of neonicotinoid imidacloprid precisely sensed and detoxified by a C-minus odorant-binding protein 17 highly expressed in the legs of Apis cerana","volume":"885","author":"Qiu","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_52","first-page":"e70008","article-title":"Insect chemosensory proteins as targets in insecticide resistance and development","volume":"2","author":"Li","year":"2025","journal-title":"New Plant Prot."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0014-5793(99)01683-X","article-title":"Disulfide structure of the pheromone binding protein from the silkworm moth, Bombyx mori","volume":"464","author":"Leal","year":"1999","journal-title":"Fed. Eur. Biochem. Soc. Lett."},{"key":"ref_54","first-page":"100605","article-title":"Genome-wide identification and expression profiling of odorant-binding proteins in the oriental fruit fly, Bactrocera dorsalis","volume":"31","author":"Chen","year":"2019","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Rond\u00f3n, J.J., Moreyra, N.N., Pisarenco, V.A., Rozas, J., Hurtado, J., and Hasson, E. (2022). Evolution of the odorant-binding protein gene family in Drosophila. Front. Ecol. Evol., 10.","DOI":"10.3389\/fevo.2022.957247"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1093\/gbe\/evr033","article-title":"Comparative Genomics of the Odorant-Binding and Chemosensory Protein Gene Families across the Arthropoda: Origin and Evolutionary History of the Chemosensory System","volume":"3","author":"Vieira","year":"2011","journal-title":"Genome Biol. Evol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Fan, Y., Zhang, C., Qin, Y., Yin, X., Dong, X., Desneux, N., and Zhou, H. (2022). Monitoring the Methyl Eugenol Response and Non-Responsiveness Mechanisms in Oriental Fruit Fly Bactrocera dorsalis in China. Insects, 13.","DOI":"10.3390\/insects13111004"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"15894","DOI":"10.1038\/s41598-017-15893-6","article-title":"BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel)","volume":"7","author":"Liu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1038\/nprot.2016.095","article-title":"Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown","volume":"11","author":"Pertea","year":"2016","journal-title":"Nat. Protoc."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1093\/bioinformatics\/btg034","article-title":"TIGR Gene Indices clustering tools (TGICL): A software system for fast clustering of large EST datasets","volume":"19","author":"Pertea","year":"2003","journal-title":"Bioinformatics"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.pestbp.2015.01.006","article-title":"Cytochrome P450s\u2014Their expression, regulation, and role in insecticide resistance","volume":"120","author":"Liu","year":"2015","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.ijbiomac.2022.07.198","article-title":"An odorant binding protein mediates Bactrocera dorsalis olfactory sensitivity to host plant volatiles and male attractant compounds","volume":"219","author":"Liu","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"103801","DOI":"10.1016\/j.ibmb.2022.103801","article-title":"BdorOBP69a is involved in the perception of the phenylpropanoid compound methyl eugenol in oriental fruit fly (Bactrocera dorsalis) males","volume":"147","author":"Chen","year":"2022","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"103656","DOI":"10.1016\/j.ibmb.2021.103656","article-title":"CRISPR\/Cas9 mutagenesis abolishes odorant-binding protein BdorOBP56f-2 and impairs the perception of methyl eugenol in Bactrocera dorsalis (Hendel)","volume":"139","author":"Chen","year":"2021","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1038\/nn.4100","article-title":"Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo","volume":"18","author":"Rossier","year":"2015","journal-title":"Nat. Neurosci."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Wu, Z., Lin, J., Zhang, H., and Zeng, X. (2016). BdorOBP83a-2 Mediates Responses of the Oriental Fruit Fly to Semiochemicals. Front. Physiol., 7.","DOI":"10.3389\/fphys.2016.00452"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1016\/j.jinsphys.2012.05.011","article-title":"Odorant receptor co-receptor Orco is upregulated by methyl eugenol in male Bactrocera dorsalis (Diptera: Tephritidae)","volume":"58","author":"Zheng","year":"2012","journal-title":"J. Insect Physiol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/4\/416\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T10:48:01Z","timestamp":1776163681000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/4\/416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,14]]},"references-count":73,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["insects17040416"],"URL":"https:\/\/doi.org\/10.3390\/insects17040416","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,14]]}}}