{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:33:14Z","timestamp":1788381194158,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32460711"],"award-info":[{"award-number":["32460711"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32460711"],"award-info":[{"award-number":["32460711"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guizhou Key Laboratory of Agricultural Biosecurity","award":["QianKeHe ZSYS [2025] 024"],"award-info":[{"award-number":["QianKeHe ZSYS [2025] 024"]}]},{"name":"Innovative Capabilities Buildup of Green Prevention and Control for Invasive Species in Agriculture","award":["QianKeHeFuQi [2023]011"],"award-info":[{"award-number":["QianKeHeFuQi [2023]011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The gene encoding juvenile hormone transcription factor Kr\u00fcppel homolog 1 (Kr-h1) was studied for its effects on ovary development and reproduction in the ladybug beetle, Coccinella septempunctata. Kr-h1 expression in C. septempunctata was evaluated in females supplied with a juvenile hormone (JH) amended diet using quantitative PCR, and the function of Kr-h1 in female reproduction was assessed using RNAi technology. Expression of Kr-h1 in females supplied with a JH diet was significantly higher at 5 and 10 d than in females not supplied with JH. At 5 and 10 d post-injection with Kr-h1-dsRNA, Kr-h1 expression levels were 30.97% and 38.32% lower, respectively, than expression in controls injected with GFP-dsRNA. Development of ovaries and vitellogenesis in ladybugs microinjected with Kr-h1-dsRNA was significantly delayed in comparison to controls. At 20 d post-injection, mean egg production decreased by 28.74% relative to controls. These results prove that Kr-h1 has a vital role in modulating ladybug fecundity by impacting ovary development and egg production.<\/jats:p>","DOI":"10.3390\/insects17060577","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:37:26Z","timestamp":1780454246000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Kr-h1 Encoding Juvenile Hormone Transcription Factor Impacts Reproductive Functions in Coccinella septempunctata"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1121-2722","authenticated-orcid":false,"given":"Ying","family":"Cheng","sequence":"first","affiliation":[{"name":"Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550006, China"},{"name":"Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Guiyang 550006, China"},{"name":"Guizhou Provincial Laboratory of Green Technology and Application Engineering of Plant Protection, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550006, China"},{"name":"Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Guiyang 550006, China"},{"name":"Guizhou Provincial Laboratory of Green Technology and Application Engineering of Plant Protection, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cao","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Pollution-Free Engineering Center of Plant Protection, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1242\/dev.033712","article-title":"Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila","volume":"136","author":"Liu","year":"2009","journal-title":"Development"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.cois.2018.07.010","article-title":"Juvenile hormone signaling in insect oogenesis","volume":"31","author":"Santos","year":"2019","journal-title":"Curr. 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