{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T07:02:07Z","timestamp":1780556527637,"version":"3.54.1"},"reference-count":70,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Tomicus destruens (Wollaston), the Mediterranean pine shoot beetle, has long been confused with Tomicus piniperda (Linnaeus) in Greece, as well as in many other Mediterranean countries. To clarify its presence and population structure, we analyzed COI sequences of specimens from 21 Greek sites (including islands) and compared them with samples from central (Italy) and western (Spain) Mediterranean populations. Greek populations were dominated by a single haplotype (Td06), whereas Samothraki Island harbored several private haplotypes, indicating long localized lineage persistence. In contrast, Italian and Spanish populations showed high haplotype diversity with many region-specific lineages, consistent with long-term refugial stability in the central-western Mediterranean. Physiological measurements further differentiate the two species: the mean supercooling point (SCP) of Greek T. destruens (\u201312.3 \u00b0C) was substantially higher than reported for T. piniperda (\u2248\u201318 \u00b0C), demonstrating lower cold tolerance. This supports its restriction to mild Mediterranean climates. Overall, our results confirm the dominant occurrence of T. destruens with no recovery of T. piniperda individuals in Greece and highlight the combined value of genetic and physiological markers for resolving species boundaries and understanding Mediterranean bark beetle biogeography.<\/jats:p>","DOI":"10.3390\/insects17060579","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:37:26Z","timestamp":1780454246000},"page":"579","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["What Really Lurks in the Dark? Revisiting the Occurrence of Tomicus destruens (Coleoptera, Curculionidae, Scolytinae) in Greece"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7772-6892","authenticated-orcid":false,"given":"Dimitrios N.","family":"Avtzis","sequence":"first","affiliation":[{"name":"Forest Research Institute, Hellenic Agricultural Organization \u00abDimitra\u00bb, 57006 Vassilika, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7158-0204","authenticated-orcid":false,"given":"Hugo","family":"Mas","sequence":"additional","affiliation":[{"name":"Laboratori de Sanitat Forestal, Centro para la Investigaci\u00f3n y Experimentaci\u00f3n Forestal de la CV, VAERSA, Conselleria de Agricultura, Generalitat Valenciana, Av. Comarques del Pa\u00eds Valenci\u00e0 114, 46930 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0402-8604","authenticated-orcid":false,"given":"Matteo","family":"Bracalini","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Food, Environment and Forestry, University of Florence, Piazzale delle Cascine, 18, 50144 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achilleas","family":"Kaltsidis","sequence":"additional","affiliation":[{"name":"Forest Research Institute, Hellenic Agricultural Organization \u00abDimitra\u00bb, 57006 Vassilika, Greece"},{"name":"Laboratory of Applied Zoology and Parasitology, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7878-1503","authenticated-orcid":false,"given":"Eleni I.","family":"Koutsogeorgiou","sequence":"additional","affiliation":[{"name":"Institute of Plant Breeding and Genetic Resources, ELGO Dimitra, 57001 Thermi, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5440-5316","authenticated-orcid":false,"given":"Stefanos S.","family":"Andreadis","sequence":"additional","affiliation":[{"name":"Institute of Plant Breeding and Genetic Resources, ELGO Dimitra, 57001 Thermi, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6532-9614","authenticated-orcid":false,"given":"Nikoleta","family":"Eleftheriadou","sequence":"additional","affiliation":[{"name":"Forest Research Institute, Hellenic Agricultural Organization \u00abDimitra\u00bb, 57006 Vassilika, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9355-0516","authenticated-orcid":false,"given":"Massimo","family":"Faccoli","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Food, Natural Resources, Animals and the Environment, University of Padua, Viale dell\u2019Universit\u00e0, 16, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","first-page":"289","article-title":"Distribution and biology of the Mediterranean pine shoot beetle Tomicus destruens (Wollaston, 1865) in the western Mediterranean region of Turkey","volume":"34","author":"Sarikaya","year":"2010","journal-title":"Turk. 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