{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T04:56:49Z","timestamp":1781326609614,"version":"3.54.1"},"reference-count":65,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32470475"],"award-info":[{"award-number":["32470475"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"General Research Project of Zhejiang Provincial Department of Education","award":["KYZ34425424"],"award-info":[{"award-number":["KYZ34425424"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Sungaya inexpectata is a tropical stick insect endemic to the Philippines, providing a useful system for investigating cold responses in tropical ectotherms. In this study, we exposed individuals to low temperature (8 \u00b0C) and normal temperature (25 \u00b0C) and characterized their transcriptomic responses. A total of 1656 differentially expressed genes were identified, including those involved in energy metabolism, cuticular proteins (CPs), and heat shock proteins. Since CP-related genes were notably enriched, we focused on this family. qPCR assessment provided preliminary expression profiles for selected candidate CP genes. Using comparative transcriptomics with eight New Zealand alpine stick insect species, we reconstructed the phylogeny of major CP families and annotated their conserved domains. Clade analysis revealed significant positive selection in the CPAP3-3 gene. In summary, this study reveals the transcriptional response of cuticular protein genes in S. inexpectata under cold exposure at 8 \u00b0C. These findings provide preliminary transcriptional data for understanding how this species responds to low temperature.<\/jats:p>","DOI":"10.3390\/insects17060604","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:43:54Z","timestamp":1780965834000},"page":"604","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cold Tolerance and Differential Expression of Cuticular Protein Genes in Sungaya inexpectata Zompro, 1996 (Insecta: Phasmatodea)"],"prefix":"10.3390","volume":"17","author":[{"given":"Kun","family":"Yang","sequence":"first","affiliation":[{"name":"College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuxiang","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijie","family":"Lin","sequence":"additional","affiliation":[{"name":"MOE Frontier Science Center for Brain Science and Brain-Machine Integration, State Key Laboratory of Brain-Machine Intelligence, Zhejiang University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingzhou","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7679-2548","authenticated-orcid":false,"given":"Jiayong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China"},{"name":"Key Lab of Wildlife Biotechnology, Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua 321004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"ref_1","first-page":"2159","article-title":"Effect of global warming on insect: A literature review","volume":"30","author":"Chen","year":"2010","journal-title":"Acta Ecol. Sin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1093\/evolut\/qpaf264","article-title":"On the repeated evolution of parthenogenesis in stick insects","volume":"80","author":"Schwander","year":"2026","journal-title":"Evolution"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1111\/ens.12467","article-title":"Outbreak of the stick insect, Ramulus mikado (Phasmatodea, Phasmatidae), in the Akashina area of Japan (Azumino City, Nagano Prefecture)","volume":"24","author":"Yano","year":"2021","journal-title":"Entomol. Sci."},{"key":"ref_4","first-page":"751","article-title":"Classes of Insect Cold Hardiness","volume":"7","author":"Bale","year":"1993","journal-title":"Funct. Ecol."},{"key":"ref_5","first-page":"849","article-title":"Insects and low temperatures: From molecular biology to distributions and abundance","volume":"357","author":"Bale","year":"2002","journal-title":"Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1017\/S000748531500098X","article-title":"The effect of rearing temperature on development, body size, energetics and fecundity of the diamondback moth","volume":"106","author":"Garrad","year":"2016","journal-title":"Bull. Entomol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/BF00379690","article-title":"Effects of cold stress during pupal diapause on the survival and development of Mamestra configurata (Lepidoptera: Noctuidae)","volume":"56","author":"Turnock","year":"1983","journal-title":"Oecologia"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"102","DOI":"10.2307\/2404603","article-title":"Effects of Sublethal Cold Stress on the Aphid Rhopalosiphum padi","volume":"31","author":"Hutchinson","year":"1994","journal-title":"J. Appl. Ecol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Denlinger, D.L., and Lee, R.E. (2010). Low Temperature Biology of Insects, Cambridge University Press.","DOI":"10.1017\/CBO9780511675997"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/syen.12472","article-title":"Reconstructing the nonadaptive radiation of an ancient lineage of ground-dwelling stick insects (Phasmatodea: Heteropterygidae)","volume":"46","author":"Bank","year":"2021","journal-title":"Syst. Entomol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1111\/mec.12767","article-title":"Divergent transcriptional responses to low temperature among populations of alpine and lowland species of New Zealand stick insects (Micrarchus)","volume":"23","author":"Dunning","year":"2014","journal-title":"Mol. Ecol."},{"key":"ref_12","first-page":"24","article-title":"Identification of cold-responsive genes in a New Zealand alpine stick insect using RNA-Seq","volume":"8","author":"Dunning","year":"2013","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"13965","DOI":"10.1038\/srep13965","article-title":"Parallel molecular routes to cold adaptation in eight genera of New Zealand stick insects","volume":"5","author":"Dennis","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/BF00613439","article-title":"Response characteristics of a cold receptor in the stick insect Carausius morosus","volume":"160","author":"Tichy","year":"1987","journal-title":"J. Comp. Physiol. A"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ibmb.2010.02.001","article-title":"Structural cuticular proteins from arthropods: Annotation, nomenclature, and sequence characteristics in the genomics era","volume":"40","author":"Willis","year":"2010","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.cois.2014.08.005","article-title":"Application of functional \u2018Omics\u2019 in environmental stress physiology: Insights, limitations, and future challenges","volume":"4","author":"Hayward","year":"2014","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s40659-015-0054-3","article-title":"Transcriptome responses to heat- and cold-stress in ladybirds (Cryptolaemus montrouzieri Mulasnt) analyzed by deep-sequencing","volume":"48","author":"Zhang","year":"2015","journal-title":"Biol. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.asd.2004.05.006","article-title":"Design and mechanical properties of insect cuticle","volume":"33","author":"Vincent","year":"2004","journal-title":"Arthropod Struct. Dev."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Chaudhari, S.S., Moussian, B., Specht, C.A., Arakane, Y., Kramer, K.J., Beeman, R.W., and Muthukrishnan, S. (2014). Functional specialization among members of Knickkopf family of proteins in insect cuticle organization. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004537"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Jasrapuria, S., Specht, C.A., Kramer, K.J., Beeman, R.W., and Muthukrishnan, S. (2012). Gene families of cuticular proteins analogous to peritrophins (CPAPs) in Tribolium castaneum have diverse functions. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0049844"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.ibmb.2007.03.011","article-title":"CPF and CPFL, two related gene families encoding cuticular proteins of Anopheles gambiae and other insects","volume":"37","author":"Togawa","year":"2007","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/imb.12777","article-title":"Cuticular protein genes involve heat acclimation of insect larvae under global warming","volume":"31","author":"Guo","year":"2022","journal-title":"Insect Mol. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.semcdb.2011.12.004","article-title":"The evolution of gene expression and the transcriptome\u2013phenotype relationship","volume":"23","author":"Harrison","year":"2012","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3044","DOI":"10.1073\/pnas.1318797111","article-title":"The predictability of molecular evolution during functional innovation","volume":"111","author":"Blank","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1152\/physiolgenomics.00042.2012","article-title":"Combined transcriptomic and metabolomic approach uncovers molecular mechanisms of cold tolerance in a temperate flesh fly","volume":"44","author":"Teets","year":"2012","journal-title":"Physiol. Genom."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: A fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"Li","year":"2006","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis","volume":"8","author":"Haas","year":"2013","journal-title":"Nat. Protoc."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Li, B., and Dewey, C.N. (2011). RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1467-985X.2010.00676_9.x","article-title":"ggplot2: Elegant graphics for data analysis","volume":"174","author":"Ginestet","year":"2011","journal-title":"J. R. Stat. Soc. Ser. A Stat. Soc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and sensitive protein alignment using DIAMOND","volume":"12","author":"Buchfink","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Eddy, S.R. (2011). Accelerated profile HMM searches. PLoS Comput. Biol., 7.","DOI":"10.1371\/journal.pcbi.1002195"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D412","DOI":"10.1093\/nar\/gkaa913","article-title":"Pfam: The protein families database in 2021","volume":"49","author":"Mistry","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the unification of biology","volume":"25","author":"Botstein","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: An integrative toolkit developed for interactive analyses of big biological data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol. Plant"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1186\/s13059-019-1832-y","article-title":"OrthoFinder: Phylogenetic orthology inference for comparative genomics","volume":"20","author":"Emms","year":"2019","journal-title":"Genome Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"W116","DOI":"10.1093\/nar\/gki442","article-title":"InterProScan: Protein domains identifier","volume":"33","author":"Quevillon","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ibmb.2014.06.004","article-title":"CutProtFam-Pred: Detection and classification of putative structural cuticular proteins from sequence alone, based on profile Hidden Markov Models","volume":"52","author":"Ioannidou","year":"2014","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/j.ibmb.2007.03.007","article-title":"Drosophila cuticular proteins with the R&R Consensus: Annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences","volume":"37","author":"Karouzou","year":"2007","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_43","first-page":"393","article-title":"Leveraging Reinforcement Learning and GBlocks for Interactive Analysis of Conserved HIV-1 Regions","volume":"11","author":"Jangade","year":"2025","journal-title":"GRADIVA Rev. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent updates to the phylogenetic tree display and annotation tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1111\/1755-0998.13096","article-title":"PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies","volume":"20","author":"Zhang","year":"2020","journal-title":"Mol. Ecol. Resour."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1002\/ece3.5015","article-title":"EasyCodeML: A visual tool for analysis of selection using CodeML","volume":"9","author":"Gao","year":"2019","journal-title":"Ecol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1093\/molbev\/msr311","article-title":"An improved likelihood ratio test for detecting site-specific functional divergence among clades of protein-coding genes","volume":"29","author":"Weadick","year":"2012","journal-title":"Mol. Biol. Evol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Cheng, D., Zhang, Z., He, X., and Liang, G. (2013). Validation of reference genes in Solenopsis invicta in different developmental stages, castes and tissues. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057718"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"L\u00fc, J., Yang, C., Zhang, Y., and Pan, H. (2018). Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: A systematic review. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.01560"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"318","DOI":"10.2144\/98242pf02","article-title":"Primer premier: Program for design of degenerate primers from a protein sequence","volume":"24","author":"Singh","year":"1998","journal-title":"BioTechniques"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Cronk, B.C. (2016). How to Use IBM SPSS Statistics: A Step-by-Step Guide to Analysis and Interpretation, Routledge.","DOI":"10.4324\/9781315142999"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/jen.12728","article-title":"Comparative transcriptome analysis of Locusta migratoria tibetensis Chen (Orthoptera: Oedipodidae) under high- and low-temperature stress","volume":"144","author":"Ran","year":"2020","journal-title":"J. Appl. Entomol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/jisesa\/iez109","article-title":"Comparative analysis of transcriptome responses to cold stress in Galeruca daurica (Coleoptera: Chrysomelidae)","volume":"19","author":"Zhou","year":"2019","journal-title":"J. Insect Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2726","DOI":"10.1111\/mec.14067","article-title":"Comparative analysis of the transcriptional responses to low and high temperatures in three rice planthopper species","volume":"26","author":"Huang","year":"2017","journal-title":"Mol. Ecol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"4634","DOI":"10.1021\/pr200518y","article-title":"Elucidating the biochemical overwintering adaptations of Larval Cucujus clavipes puniceus, a nonmodel organism, via high throughput proteomics","volume":"10","author":"Carrasco","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/j.1365-2583.2005.00589.x","article-title":"Cold hardening and transcriptional change in Drosophila melanogaster","volume":"14","author":"Qin","year":"2005","journal-title":"Insect Mol. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4486","DOI":"10.1038\/s41467-025-59723-0","article-title":"3,4-Dihydroxyphenylacetaldehyde synthase evolved an ordered structure to deliver oxygen to pyridoxal 5\u2019-phosphate for cuticle assembly in the mosquito Aedes aegypti","volume":"16","author":"Chen","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Cui, M., Hu, P., Wang, T., Tao, J., and Zong, S. (2017). Differential transcriptome analysis reveals genes related to cold tolerance in seabuckthorn carpenter moth, Eogystia hippophaecolus. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0187105"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1016\/S0022-2275(20)41163-0","article-title":"Purification and properties of a phosphorylatable triacylglycerol lipase from the fat body of an insect, Manduca sexta","volume":"35","author":"Arrese","year":"1994","journal-title":"J. Lipid Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e12563","DOI":"10.7717\/peerj.12563","article-title":"The metabolism and role of free fatty acids in key physiological processes in insects of medical, veterinary and forensic importance","volume":"9","author":"Kaczmarek","year":"2021","journal-title":"PeerJ"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2656","DOI":"10.1194\/jlr.M600161-JLR200","article-title":"The main triglyceride-lipase from the insect fat body is an active phospholipase A1: Identification and characterization","volume":"47","author":"Arrese","year":"2006","journal-title":"J. Lipid Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0022-1910(00)00099-8","article-title":"Relationships between cold hardiness and diapause, and between glycerol and free amino acid contents in overwintering larvae of the oriental corn borer, Ostrinia furnacalis","volume":"47","author":"Goto","year":"2001","journal-title":"J. Insect Physiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1111\/1744-7917.12869","article-title":"Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae)","volume":"28","author":"Hou","year":"2021","journal-title":"Insect Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"17028","DOI":"10.1073\/pnas.1112288108","article-title":"Knickkopf protein protects and organizes chitin in the newly synthesized insect exoskeleton","volume":"108","author":"Chaudhari","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/6\/604\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T04:17:37Z","timestamp":1781324257000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/6\/604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,8]]},"references-count":65,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["insects17060604"],"URL":"https:\/\/doi.org\/10.3390\/insects17060604","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,8]]}}}