{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:23:01Z","timestamp":1787239381462,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Large-scale breeding and resource utilization of BSF","award":["H20439"],"award-info":[{"award-number":["H20439"]}]},{"DOI":"10.13039\/501100021171","name":"GuangDong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2021A1515010907"],"award-info":[{"award-number":["2021A1515010907"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The impacts of various feed substrates and processing treatments (boiling and extrusion) designed to reduce plant-derived antinutritional factors on the growth and development, nutritional composition, and intestinal microbiota of black soldier fly larvae (BSFL) were evaluated. The research revealed that, except for the soybean flour group, the cottonseed meal group and the treatment group with antinutritional factors removed showed better growth trends compared to the Gainesville House Fly Diet group. The heat-processed groups had higher maximum larval weights, survival rates, and feed conversion rates than the untreated groups. Additionally, BSFL fed with high-protein and high-fat experimental diets generally exhibited higher fat and protein contents. Plant-derived antinutritional factors in the test substrates significantly affected the intestinal microbiota and their metabolic pathways in BSFL. Gut microbiota\u2014including phyla such as Firmicutes, Proteobacteria, and Bacteroidetes, along with various lactic acid\u2014and short-chain fatty acid-producing genera\u2014and metabolic pathways related to carbohydrates, amino acids, cofactors, and vitamins, play crucial roles in helping BSFL resist plant antinutritional factors.<\/jats:p>","DOI":"10.3390\/insects17070675","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:11:57Z","timestamp":1782785517000},"page":"675","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Soybean- and Cottonseed-Based Diets on Growth Performance and Gut Microbiota of Black Soldier Fly Larvae"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3277-633X","authenticated-orcid":false,"given":"Xingbao","family":"Feng","sequence":"first","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"College of Biological and Food Engineering, Hubei Minzu University, Enshi 445000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianru","family":"Mo","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxuan","family":"Jian","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6670-3062","authenticated-orcid":false,"given":"Ling","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kaya, C., Generalovic, T.N., St\u00e5hls, G., Hauser, M., Samayoa, A.C., Nunes-Silva, C.G., Roxburgh, H., Wohlfahrt, J., Ewusie, E.A., and Kenis, M. (2021). Global population genetic structure and demographic trajectories of the black soldier fly, Hermetia illucens. BMC Biol., 19.","DOI":"10.1186\/s12915-021-01029-w"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12701","DOI":"10.1007\/s13762-021-03524-7","article-title":"Sustainable waste management using black soldier fly larva: A review","volume":"19","author":"Purkayastha","year":"2022","journal-title":"Int. J. Environ. Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s13744-019-00719-z","article-title":"A Review of the Use of Black Soldier Fly Larvae, Hermetia illucens (Diptera: Stratiomyidae), to Compost Organic Waste in Tropical Regions","volume":"49","author":"Hesselberg","year":"2020","journal-title":"Neotrop. Entomol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1163\/23524588-00001439","article-title":"Selective breeding of heat-tolerant black soldier fly (Hermetia illucens) larvae: Gut microbial shifts and transcriptional patterns","volume":"11","author":"Feng","year":"2025","journal-title":"J. Insects Food Feed."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.wasman.2024.02.007","article-title":"Selective breeding of cold-tolerant black soldier fly (Hermetia illucens) larvae: Gut microbial shifts and transcriptional patterns","volume":"177","author":"Ma","year":"2024","journal-title":"Waste. Manag."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"103260","DOI":"10.1016\/j.eti.2023.103260","article-title":"Exploring insect-based technology for waste management and livestock feeding in selected South and East Asian countries","volume":"32","author":"Salam","year":"2023","journal-title":"Environ. Technol. Innov."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3390\/biology12010025","article-title":"The Variety of Applications of Hermetia illucens in Industrial and Agricultural Areas-Review","volume":"12","author":"Kaczor","year":"2023","journal-title":"Biology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s40104-019-0358-1","article-title":"Hermetia illucens larvae as a potential dietary protein source altered the microbiota and modulated mucosal immune status in the colon of finishing pigs","volume":"10","author":"Yu","year":"2019","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1007\/s10311-019-00904-x","article-title":"Applications of chitosan in food, pharmaceuticals, medicine, cosmetics, agriculture, textiles, pulp and paper, biotechnology, and environmental chemistry","volume":"17","author":"Lichtfouse","year":"2019","journal-title":"Environ. Chem. Lett."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.ijbiomac.2019.10.113","article-title":"Chitosan as an environment friendly biomaterial-a review on recent modifications and applications","volume":"150","author":"Bakshi","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/gcbb.12470","article-title":"A comparison in product-value potential in four treatment strategies for food waste and faeces-assessing composting, fly larvae composting and anaerobic digestion","volume":"10","author":"Lalander","year":"2018","journal-title":"GCB Bioenergy"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"115529","DOI":"10.1016\/j.jenvman.2022.115529","article-title":"Valorization of pretreated biogas digestate with black soldier fly (Hermetia illucens, L.; Diptera: Stratiomyidae) larvae","volume":"319","author":"Pas","year":"2022","journal-title":"J. Environ. Manag."},{"key":"ref_13","first-page":"2676","article-title":"Performance of the black soldier fly (Diptera: Stratiomyidae) on vegetable residue-based diets formulated based on protein and carbohydrate contents","volume":"111","author":"Dicke","year":"2018","journal-title":"J. Econ. Entomol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bian, H., Qiao, Y., Li, Y., Wang, Z., Zhao, L., Li, Z., Cheng, B., and Ding, G. (2024). The Growth Performance and Nutrient Composition of Black Soldier Fly (Hermetia illucens) Larvae Fed Slaughtered Bovine Blood. Insects, 15.","DOI":"10.3390\/insects15090635"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Belperio, S., Cattaneo, A., Nannoni, E., Sardi, L., Martelli, G., Dabbou, S., and Meneguz, M. (2024). Assessing Substrate Utilization and Bioconversion Efficiency of Black Soldier Fly (Hermetia illucens) Larvae: Effect of Diet Composition on Growth and Development Temperature. Animals, 14.","DOI":"10.20944\/preprints202403.1268.v1"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1603\/0022-2585-39.4.695","article-title":"Rearing methods for the black soldier fly (Diptera: Stratiomyidae)","volume":"39","author":"Sheppard","year":"2002","journal-title":"J. Med. Entomol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bukan, M., Andrijani\u0107, Z., Peji\u0107, I., Klju\u010dari\u0107, M., \u010ci\u017emek, L., Tomaz, I., Buljevi\u0107, N., and \u0160ar\u010devi\u0107, H. (2024). Validation of Molecular Markers for Low Kunitz Trypsin Inhibitor Content in European Soybean (Glycine max L. Merr.) Germplasm. Genes, 15.","DOI":"10.3390\/genes15081028"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100726","DOI":"10.1016\/j.fochx.2023.100726","article-title":"Ultra-high-pressure passivation of soybean agglutinin and safety evaluation","volume":"18","author":"Han","year":"2023","journal-title":"Food Chem. X"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Song, J.H., Shin, G., Kim, H.J., Lee, S.B., Moon, J.Y., Jeong, J.C., Choi, H., Kim, I.A., Song, H., and Kim, C.Y. (2022). Mutation of GmIPK1 gene using CRISPR\/Cas9 reduced phytic acid content in soybean seeds. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810583"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Yan, Z., Li, T., Zou, G., Zhang, X., Qu, L., and Wei, Y. (2025). Probiotic Fermentation of Defatted Cottonseed Meal for Sustainable Foods and Non-Food Applications. Microorganisms, 13.","DOI":"10.3390\/microorganisms13051020"},{"key":"ref_21","unstructured":"China Agricultural Sciences Academy Beijing Veterinary Medicine Research Institute, and National Key Laboratory of Animal Husbandry and Poultry Nutrition and Breeding (2024). Explanation for the formulation of China Feed Composition and Nutritional Value Table. China Feed, 1, 181."},{"key":"ref_22","unstructured":"(2018). Determination of crude protein in feed\u2014Kjeldahl method (Standard No. GB\/T 6432-2018)."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1007\/s11694-022-01628-3","article-title":"Monitoring of critical parameters in thermophilic solid-state fermentation process of soybean meal using NIR spectroscopy and chemometrics","volume":"17","author":"Dai","year":"2023","journal-title":"J. Food Meas. Charact."},{"key":"ref_24","unstructured":"(2006). Determination of crude fat in feed (Standard No. GB\/T 6433-2006)."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jclepro.2018.10.017","article-title":"Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens)","volume":"208","author":"Lalander","year":"2019","journal-title":"J. Clean. Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1093\/ee\/nvv002","article-title":"Ability of black soldier fly (Diptera: Stratiomyidae) larvae to recycle food waste","volume":"44","author":"Nguyen","year":"2015","journal-title":"Environ. Entomol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/ee\/37.1.11","article-title":"Development of black soldier fly (Diptera: Stratiomyidae) larvae fed dairy manure","volume":"37","author":"Myers","year":"2008","journal-title":"Environ. Entomol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1603\/ME13021","article-title":"Developmental and waste reduction plasticity of three black soldier fly strains (Diptera: Stratiomyidae) raised on different livestock manures","volume":"50","author":"Zhou","year":"2013","journal-title":"J. Med. Entomol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/tmi.12228","article-title":"Growth rates of black soldier fly larvae fed on fresh human faeces and their implication for improving sanitation","volume":"19","author":"Banks","year":"2014","journal-title":"Trop. Med. Int. Health"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1007\/s00253-023-12857-x","article-title":"Intestinal microbial community well explain larval growth than feed types","volume":"108","author":"Li","year":"2024","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3920\/JIFF2016.0055","article-title":"Nutritional value of the black soldier fly (Hermetia illucens L.) and its suitability as animal feed-a review","volume":"3","author":"Dicke","year":"2017","journal-title":"J. Insects Food Feed."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Oonincx, D.G.A.B., Van Broekhoven, S., Van Huis, A., and van Loon, J.J.A. (2015). Feed conversion, survival and development, and composition of four insect species on diets composed of food by-products. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0144601"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.aspen.2017.09.017","article-title":"Metabolic adjustment of the larval fat body in Hermetia illucens to dietary conditions","volume":"20","author":"Pimentel","year":"2017","journal-title":"J. Asia-Pac. Entomol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ashayerizadeh, A., Jazi, V., Sharifi, F., Toghyani, M., Mohebodini, H., Kim, I.H., and Roura, E. (2024). Fermented but not irradiated cottonseed meal has the potential to partially substitute soybean meal in broiler chickens. Animals, 14.","DOI":"10.3390\/ani14192797"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.2527\/2004.8241108x","article-title":"Effect of soybean variety and processing on growth performance of young chicks and pigs","volume":"82","author":"Palacios","year":"2004","journal-title":"J. Anim. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.aninu.2024.03.020","article-title":"Research progress on cottonseed meal as a protein source in pig nutrition: An updated review","volume":"18","author":"Tao","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4436","DOI":"10.1007\/s13197-022-05523-z","article-title":"The effect of radiofrequency heat treatment on trypsin inhibitor activity and in vitro digestibility of soybean varieties (Glycine max. (L.) Merr.)","volume":"59","author":"Nagy","year":"2022","journal-title":"J. Food Sci. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Xiao, R., Rosso, L., Walker, T., Reilly, P., Zhang, B., and Huang, H. (2025). Reducing Heat Without Impacting Quality: Optimizing Trypsin Inhibitor Inactivation Process in Low-TI Soybean. Foods, 14.","DOI":"10.3390\/foods14173039"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"skaa224","DOI":"10.1093\/jas\/skaa224","article-title":"Thermomechanical and enzyme-facilitated processing of soybean meal enhanced in vitro kinetics of protein digestion and protein and amino acid digestibility in weaned pigs","volume":"98","author":"Lupatsch","year":"2020","journal-title":"J. Anim. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.aninu.2018.05.007","article-title":"Processing techniques of selected oilseed by-products of potential use in animal feed: Effects on proximate nutrient composition, amino acid profile and antinutrients","volume":"4","author":"Duodu","year":"2018","journal-title":"Anim. Nutr."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1603\/EN10126","article-title":"Inoculating poultry manure with companion bacteria influences growth and development of black soldier fly (Diptera: Stratiomyidae) larvae","volume":"40","author":"Yu","year":"2011","journal-title":"Environ. Entomol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1007\/s00248-018-1286-x","article-title":"Assessing the microbiota of black soldier fly larvae (Hermetia illucens) reared on organic waste streams on four different locations at laboratory and large scale","volume":"77","author":"Wynants","year":"2019","journal-title":"Microb. Ecol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"136898","DOI":"10.1016\/j.jhazmat.2024.136898","article-title":"Alkane degradation coupled to Fe (III) reduction mediated by Gram-positive bacteria","volume":"486","author":"Liu","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"100471","DOI":"10.1016\/j.clinsp.2024.100471","article-title":"Firmicutes\/Bacteroidetes and Firmicutes\/Proteobacteria ratios are associated with worse prognosis in a cohort of Latin American patients with cirrhosis","volume":"79","author":"Mantovani","year":"2024","journal-title":"Clinics"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Rabee, A.E., Abd El Rahman, T., and Lamara, M. (2023). Changes in the bacterial community colonizing extracted and non-extracted tannin-rich plants in the rumen of dromedary camels. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0282889"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1039\/D2FO02095E","article-title":"Modified cereal bran (MCB) from finger millet, kodo millet, and rice bran prevents high-fat diet-induced metabolic derangements","volume":"14","author":"Devi","year":"2023","journal-title":"Food Funct."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Gong, T., Ji, M., Yang, Y., Liu, J., Gong, Y., Liu, S., Zhao, Y., Cao, G., Guo, X., and Yang, Y. (2024). Enzymatically hydrolyzed diet improves growth performance and intestinal microbiome in growing pigs. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1485017"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e03616-22","DOI":"10.1128\/spectrum.03616-22","article-title":"Week-old chicks with high Bacteroides abundance have increased short-chain fatty acids and reduced markers of gut inflammation","volume":"11","author":"Fan","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Kuang, B., Xiao, R., Hu, Y., Wang, Y., Zhang, L., Wei, Z., Bai, J., Zhang, K., Acu\u00f1a, J.J., and Jorquera, M.A. (2023). Metagenomics reveals biogeochemical processes carried out by sediment microbial communities in a shallow eutrophic freshwater lake. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1112669"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Wan, J., Wang, X., Yang, T., Wei, Z., Banerjee, S., Friman, V.P., Mei, X., Xu, Y., and Shen, Q. (2021). Livestock manure type affects microbial community composition and assembly during composting. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.621126"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"119026","DOI":"10.1016\/j.ecoenv.2025.119026","article-title":"Integrated field-scale natural composite oxidation pond system for livestock wastewater treatment: Microbial insights and nutrient removal dynamics","volume":"303","author":"Wang","year":"2025","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5480","DOI":"10.1002\/fsn3.4195","article-title":"Fermenting kale (Brassica oleracea L.) enhances its functional food properties by increasing accessibility of key phytochemicals and reducing antinutritional factors","volume":"12","author":"Subedi","year":"2024","journal-title":"Food Sci. Nutr."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7413","DOI":"10.1039\/D3FO01547E","article-title":"Characteristics of the microstructure and the key components of white kidney bean sourdough bread induced by mixed-strain fermentation and its influence on gut microbiota","volume":"14","author":"Chen","year":"2023","journal-title":"Food Funct."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"105616","DOI":"10.1016\/j.psj.2025.105616","article-title":"Replacement of soybean meal with fermented rapeseed meal in broiler diets: Impacts on growth performance, gut health, and nutrient digestibility","volume":"104","author":"Dastar","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.aninu.2021.11.005","article-title":"Gut microbiota-derived short chain fatty acids are potential mediators in gut inflammation","volume":"8","author":"Akhtar","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.chom.2023.03.016","article-title":"Cross-feeding in the gut microbiome: Ecology and mechanisms","volume":"31","author":"Culp","year":"2023","journal-title":"Cell Host Microbe"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e70035","DOI":"10.1002\/imt2.70035","article-title":"Single-microbe RNA sequencing uncovers unexplored specialized metabolic functions of keystone species in the human gut","volume":"4","author":"Shen","year":"2025","journal-title":"iMeta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11274-025-04288-9","article-title":"The multifaceted roles of gut microbiota in insect physiology, metabolism, and environmental adaptation: Implications for pest management strategies","volume":"41","author":"Haider","year":"2025","journal-title":"World J. Microbiol. Biotechnol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/7\/675\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T04:41:45Z","timestamp":1782967305000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/7\/675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,28]]},"references-count":58,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["insects17070675"],"URL":"https:\/\/doi.org\/10.3390\/insects17070675","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,28]]}}}