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In this study, the repellent rate was significantly decreased by about 20% when the PxylPBP2 was silenced using RNAi. There was obvious interaction between 2,3-dimethyl-6-(1-hydroxy)-pyrazine and PxylPBP2, with a Kd (Dissociation constant) value of 7.78 \u03bcmol\/L via microscale thermophoresis, while the value was changed to 4.45 \u03bcmol\/L when the PxylOR31 (Pxyl, Plutella xylostella; OR31, Odorant receptor 31) was introduced to this compound and ligand system. Moreover, the RMSD (Root mean square deviation) value of pyrazine + PxylPBP2 + PxylOR31 treatment ranged between 0.030 and 0.125 nm; the average number of hydrogen bonds and Van der Waals interaction value can be used to summarize that there was a stronger interaction between pyrazine and PxylPBP2. Furthermore, the ILE122 (L-Isoleucine at 122 site) was an important target site during the binding process between PxylBPB2 and ligands, which was validated by performing a fluorescence competitive binding experiment. Finally, the PxylPBP2 showed a strong ability to bind to \u03b1-pinene and \u03b2-ionone with the Ki values of 10.54 \u03bcmol\/L and 11.74 \u03bcmol\/L, respectively. These data provide a creative strategy for developing novel insecticides, which is also important for achieving the green control of P. xylostella and delaying the development of resistance.<\/jats:p>","DOI":"10.3390\/insects17070708","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:55:32Z","timestamp":1783598132000},"page":"708","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Analysis of PxylPBP2 Responding to Repellent Activity of Natural Pyrazine Against Diamondback Moth"],"prefix":"10.3390","volume":"17","author":[{"given":"Yan-Lu","family":"Zhou","sequence":"first","affiliation":[{"name":"Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China"},{"name":"Key Laboratory of Agri-Products Quality and Biosafety (Anhui Agricultural University), 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