{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:58:31Z","timestamp":1785412711371,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Chinese National Natural Science Foundation","doi-asserted-by":"publisher","award":["32371886"],"award-info":[{"award-number":["32371886"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32371886"],"award-info":[{"award-number":["32371886"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"National Key R & D Program of China","doi-asserted-by":"publisher","award":["2022YFD1401000"],"award-info":[{"award-number":["2022YFD1401000"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Ecological control studies of the Asian longhorned beetle (Anoplophora glabripennis) distinguish susceptible, resistant, and dead-end trap trees as functionally different plant categories. We used shotgun metagenomics to examine bacterial and archaeal profiles detected in adult gut samples after 72 h exposure to three dietary plants or prolonged water-only starvation. The study included 24 metagenomes, with three biological replicates per DietGroup \u00d7 SexGroup combination. No time-zero gut samples were available, so the observed patterns remain superimposed on the beetles\u2019 field history. The retained catalogue contained 152,895 bacterial genes and 9 archaeal genes. The original observed-richness difference was strongly correlated with host-depleted read depth and was not supported after common-depth rarefaction. Genus-level Bray\u2013Curtis analysis detected a DietGroup \u00d7 SexGroup interaction that persisted after depth adjustment and exclusion of low-yield samples. This interaction was exploratory because of the small within-cell sample size. Raw Bray\u2013Curtis analysis of KEGG Orthology profiles showed a DietGroup association, but this association was not robust to direct-depth adjustment or Aitchison analysis. CAZy profiles were descriptive and showed no significant DietGroup effect. These results indicate short-term, depth-sensitive associations between dietary treatment and gut-sample bacterial and archaeal profiles. They do not establish resident status, microbial activity, or a physiological mechanism.<\/jats:p>","DOI":"10.3390\/insects17080756","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:13:17Z","timestamp":1784826797000},"page":"756","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Short-Term Feeding on Ecologically Distinct Dietary Plants Is Associated with Gut-Sample Bacterial and Archaeal Profiles in Adult Anoplophora glabripennis"],"prefix":"10.3390","volume":"17","author":[{"given":"Hanshuo","family":"Liu","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1728-1763","authenticated-orcid":false,"given":"Ruohan","family":"Qi","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Tian","sequence":"additional","affiliation":[{"name":"Shijingshan District, Beijing Gardening and Greening Bureau, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0333-0681","authenticated-orcid":false,"given":"Lili","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youqing","family":"Luo","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1146\/annurev-ento-112408-085427","article-title":"Managing invasive populations of Asian longhorned beetle and citrus longhorned beetle: A worldwide perspective","volume":"55","author":"Haack","year":"2010","journal-title":"Annu. Rev. Entomol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1461-9563.2009.00443.x","article-title":"Ecology and management of exotic and endemic Asian longhorned beetle Anoplophora glabripennis","volume":"11","author":"Hu","year":"2009","journal-title":"Agric. For. Entomol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1111\/1744-7917.13187","article-title":"Current and future control of the wood-boring pest Anoplophora glabripennis","volume":"30","author":"Wang","year":"2023","journal-title":"Insect Sci."},{"key":"ref_4","first-page":"51","article-title":"Behavior of Anoplophora glabripennis adults","volume":"36","author":"He","year":"1993","journal-title":"Acta Entomol. Sin."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1111\/1365-2656.13063","article-title":"Divergent host plant utilization by adults and offspring is related to intra-plant variation in chemical defences","volume":"88","author":"Mason","year":"2019","journal-title":"J. Anim. Ecol."},{"key":"ref_6","first-page":"31","article-title":"Research foundations and prospects for ecological self-control of Asian longhorned beetle disasters","volume":"62","author":"Li","year":"2025","journal-title":"Chin. J. Appl. Entomol."},{"key":"ref_7","first-page":"44","article-title":"Planting model of dead-end trap tree Elaeagnus angustifolia and resistant host tree Populus alba var. pyramidalis for ecological self-regulation of the Anoplophora glabripennis disaster","volume":"43","author":"Yang","year":"2024","journal-title":"For. Pest Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.phytochem.2013.11.009","article-title":"Using plant chemistry and insect preference to study the potential of Barbarea (Brassicaceae) as a dead-end trap crop for diamondback moth (Lepidoptera: Plutellidae)","volume":"98","author":"Reichelt","year":"2014","journal-title":"Phytochemistry"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.0013-8703.2004.00120.x","article-title":"Host tree resistance against the polyphagous wood-boring beetle Anoplophora glabripennis","volume":"110","author":"Morewood","year":"2004","journal-title":"Entomol. Exp. Appl."},{"key":"ref_10","first-page":"89","article-title":"Comparison of the resistant characteristics of Populus alba var. pyramidalis and Populus deltoides CL Beikang to damages against Anoplophora glabripennis (Coleoptera: Cerambycidae)","volume":"54","author":"Wang","year":"2018","journal-title":"Sci. Silvae Sin."},{"key":"ref_11","first-page":"23","article-title":"Preliminary study on the trap function of Elaeagnus angustifolia on Anoplophora glabripennis population","volume":"4","author":"Tian","year":"2003","journal-title":"J. Inn. Mong. For. Sci. Technol."},{"key":"ref_12","first-page":"85","article-title":"Selection of trap trees for controlling Anoplophora glabripennis and A. nobilis","volume":"4","author":"Li","year":"1999","journal-title":"J. Beijing For. Univ."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Li, C., Pei, J., Wang, L., Tian, Y., Ren, L., and Luo, Y. (2024). Interactions at the oviposition scar: Molecular and metabolic insights into Elaeagnus angustifolia\u2019s resistance response to Anoplophora glabripennis. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25179504"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Qi, R., Pei, J., Zhou, Q., Hao, K., Tian, Y., Ren, L., and Luo, Y. (2024). Comparative metabolic defense responses of three tree species to the supplemental feeding behavior of Anoplophora glabripennis. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252312716"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev.ento.49.061802.123416","article-title":"The gut bacteria of insects: Nonpathogenic interactions","volume":"49","author":"Dillon","year":"2004","journal-title":"Annu. Rev. Entomol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/1574-6976.12025","article-title":"The gut microbiota of insects\u2014Diversity in structure and function","volume":"37","author":"Engel","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1146\/annurev-ento-010814-020822","article-title":"Multiorganismal insects: Diversity and function of resident microorganisms","volume":"60","author":"Douglas","year":"2015","journal-title":"Annu. Rev. Entomol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00442-015-3327-1","article-title":"Gut microbes may facilitate insect herbivory of chemically defended plants","volume":"179","author":"Hammer","year":"2015","journal-title":"Oecologia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/nrmicro3182","article-title":"Symbiotic digestion of lignocellulose in termite guts","volume":"12","author":"Brune","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12932","DOI":"10.1073\/pnas.0805257105","article-title":"Lignin degradation in wood-feeding insects","volume":"105","author":"Geib","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Scully, E.D., Geib, S.M., Hoover, K., Tien, M., Tringe, S.G., Barry, K.W., Glavina del Rio, T., Chovatia, M., Herr, J.R., and Carlson, J.E. (2013). Metagenomic profiling reveals lignocellulose degrading system in a microbial community associated with a wood-feeding beetle. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0073827"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Scully, E.D., Geib, S.M., Carlson, J.E., Tien, M., McKenna, D., and Hoover, K. (2014). Functional genomics and microbiome profiling of the Asian longhorned beetle Anoplophora glabripennis reveal insights into the digestive physiology and nutritional ecology of wood-feeding beetles. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-1096"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1186\/s13059-016-1088-8","article-title":"Genome of the Asian longhorned beetle Anoplophora glabripennis, a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle\u2013plant interface","volume":"17","author":"McKenna","year":"2016","journal-title":"Genome Biol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wang, L., Li, C., Wang, X., Wang, G., Shang, S., Dou, Z., and Luo, Y. (2022). Gut lignocellulose activity and microbiota in Asian longhorned beetle and their predicted contribution to larval nutrition. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.899865"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1007\/s00248-018-1205-1","article-title":"Bacterial and fungal midgut community dynamics and transfer between mother and brood in the Asian longhorned beetle Anoplophora glabripennis, an invasive xylophage","volume":"77","author":"Mason","year":"2019","journal-title":"Microb. Ecol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"420287","DOI":"10.1155\/2013\/420287","article-title":"Characterization of the bacterial community associated with larvae and adults of Anoplophora chinensis collected in Italy by culture and culture-independent methods","volume":"2013","author":"Rizzi","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_27","first-page":"1750","article-title":"Host adaptability of Asian longhorn beetle Anoplophora glabripennis based on metabolomics and insect intestinal bacteria","volume":"49","author":"Gu","year":"2022","journal-title":"J. Plant Prot."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4704","DOI":"10.1002\/ece3.3977","article-title":"The influence of diet and environment on the gut microbial community of field crickets","volume":"8","author":"Ng","year":"2018","journal-title":"Ecol. Evol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4500","DOI":"10.1038\/ncomms5500","article-title":"Individual diet has sex-dependent effects on vertebrate gut microbiota","volume":"5","author":"Bolnick","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1038\/nbt.3935","article-title":"Shotgun metagenomics, from sampling to analysis","volume":"35","author":"Quince","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3468","DOI":"10.1128\/AEM.00068-13","article-title":"Mountain pine beetles colonizing historical and na\u00efve host trees are associated with a bacterial community highly enriched in genes contributing to terpene metabolism","volume":"79","author":"Adams","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1007\/s10886-013-0313-0","article-title":"Bacteria associated with a tree-killing insect reduce concentrations of plant defense compounds","volume":"39","author":"Boone","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1007\/s00442-014-2950-6","article-title":"Plant-associated bacteria degrade defense chemicals and reduce their adverse effects on an insect defoliator","volume":"175","author":"Mason","year":"2014","journal-title":"Oecologia"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4099","DOI":"10.1111\/mec.14186","article-title":"Gut microbiota of the pine weevil degrades conifer diterpenes and increases insect fitness","volume":"26","author":"Berasategui","year":"2017","journal-title":"Mol. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7618","DOI":"10.1038\/ncomms8618","article-title":"Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee","volume":"6","author":"Vega","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1469-8137.2006.01798.x","article-title":"Genome-wide analysis of the structural genes regulating defense phenylpropanoid metabolism in Populus","volume":"172","author":"Tsai","year":"2006","journal-title":"New Phytol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1080\/07352680903241279","article-title":"Genomics of secondary metabolism in Populus: Interactions with biotic and abiotic environments","volume":"28","author":"Chen","year":"2009","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1126\/science.1217411","article-title":"The rise of chemodiversity in plants","volume":"336","author":"Weng","year":"2012","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/BF00988096","article-title":"Distribution of birch (Betula spp.), willow (Salix spp.), and poplar (Populus spp.) secondary metabolites and their potential role as chemical defense against herbivores","volume":"10","author":"Palo","year":"1984","journal-title":"J. Chem. Ecol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and accurate short read alignment with Burrows\u2013Wheeler transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: An ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph","volume":"31","author":"Li","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Hyatt, D., Chen, G.L., LoCascio, P.F., Land, M.L., Larimer, F.W., and Hauser, L.J. (2010). Prodigal: Prokaryotic gene recognition and translation initiation site identification. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-119"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: A fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"Li","year":"2006","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.1093\/bioinformatics\/btp336","article-title":"SOAP2: An improved ultrafast tool for short read alignment","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/s41592-021-01101-x","article-title":"Sensitive protein alignments at tree-of-life scale using DIAMOND","volume":"18","author":"Buchfink","year":"2021","journal-title":"Nat. Methods"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"D274","DOI":"10.1093\/nar\/gkaa1018","article-title":"COG database update: Focus on microbial diversity, model organisms, and widespread pathogens","volume":"49","author":"Galperin","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"D490","DOI":"10.1093\/nar\/gkt1178","article-title":"The carbohydrate-active enzymes database CAZy in 2013","volume":"42","author":"Lombard","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"W95","DOI":"10.1093\/nar\/gky418","article-title":"dbCAN2: A meta server for automated carbohydrate-active enzyme annotation","volume":"46","author":"Zhang","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Eddy, S.R. (2011). Accelerated profile HMM searches. PLoS Comput. Biol., 7.","DOI":"10.1371\/journal.pcbi.1002195"},{"key":"ref_52","first-page":"32","article-title":"A new method for non-parametric multivariate analysis of variance","volume":"26","author":"Anderson","year":"2001","journal-title":"Austral Ecol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/j.1541-0420.2005.00440.x","article-title":"Distance-based tests for homogeneity of multivariate dispersions","volume":"62","author":"Anderson","year":"2006","journal-title":"Biometrics"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"Segata","year":"2011","journal-title":"Genome Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1038\/s41559-018-0519-1","article-title":"Function and functional redundancy in microbial systems","volume":"2","author":"Louca","year":"2018","journal-title":"Nat. Ecol. Evol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.tim.2016.02.002","article-title":"Functional redundancy-induced stability of gut microbiota subjected to disturbance","volume":"24","author":"Moya","year":"2016","journal-title":"Trends Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Pauchet, Y., Wilkinson, P., Chauhan, R., and Ffrench-Constant, R.H. (2010). Diversity of beetle genes encoding novel plant cell wall degrading enzymes. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0015635"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.cbpa.2015.10.018","article-title":"Lignocellulose degradation mechanisms across the Tree of Life","volume":"29","author":"Cragg","year":"2015","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/bs.aiip.2019.08.001","article-title":"Plant cell wall degradation in insects: Recent progress on endogenous enzymes revealed by multi-omics technologies","volume":"57","author":"Tokuda","year":"2019","journal-title":"Adv. Insect Physiol."},{"key":"ref_60","first-page":"1179","article-title":"Cellulase activity and its relationship with host selection in the Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae)","volume":"53","author":"Li","year":"2010","journal-title":"Acta Entomol. Sin."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/756\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T17:26:33Z","timestamp":1784827593000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/756"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,23]]},"references-count":60,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["insects17080756"],"URL":"https:\/\/doi.org\/10.3390\/insects17080756","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,23]]}}}