{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:11:44Z","timestamp":1789067504830,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System","award":["CARS-01"],"award-info":[{"award-number":["CARS-01"]}]},{"award":["CARS-01"],"award-info":[{"award-number":["CARS-01"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Qiannongkeyuan Emerging Team","award":["[2026]14"],"award-info":[{"award-number":["[2026]14"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Heat shock protein 70 (HSP70) regulates the stress tolerance and reproductive ability of insects. In this study, the SfHSP70-7 gene was cloned and identified from the rice pest Sogatella furcifera. SfHSP70-7 encodes a typical cytoplasmic HSP70 protein that has a conserved functional domain and is highly similar to the HSP70 homologous protein of Laodelphax striatellus. SfHSP70-7 was found to be widely expressed across all developmental stages, with the highest levels in nymphs and female adults. In terms of tissue distribution, it was particularly abundant in the gut and ovaries. Its expression was strongly induced by high\/low temperatures and three insecticides (triflumezopyrim, sulfoxaflor, and imidacloprid), with the maximum induction under heat and triflumezopyrim stress. RNA interference (RNAi) efficiently silenced SfHSP70-7 and significantly increased the susceptibility of S. furcifera to triflumezopyrim and sulfoxaflor, but it had no effect on susceptibility to imidacloprid. RNAi knockdown of SfHSP70-7 markedly reduced survival by 45.5% (30 \u00b0C) and 38.9% (35 \u00b0C) under heat stress. In addition, gene knockdown can damage the reproductive performance of females by reducing oviposition and egg hatchability. These results indicate that SfHSP70-7 is involved in regulating the heat tolerance, insecticide adaptability, and reproductive regulation mechanism of S. furcifera, providing a potential target for pest control.<\/jats:p>","DOI":"10.3390\/insects17080765","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:08:14Z","timestamp":1785161294000},"page":"765","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Roles of SfHSP70-7 in Thermal Tolerance, Insecticide Adaptability, and Reproductive Performance Under Combined Environmental Stress of the Rice Pest Sogatella furcifera (Hemiptera: Delphacidae)"],"prefix":"10.3390","volume":"17","author":[{"given":"Zhenzhen","family":"Wang","sequence":"first","affiliation":[{"name":"Guizhou Rice Research Institute, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6641-0983","authenticated-orcid":false,"given":"Yi","family":"Yan","sequence":"additional","affiliation":[{"name":"Guizhou Key Laboratory of Agricultural Biosecurity, College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongfen","family":"Yi","sequence":"additional","affiliation":[{"name":"Guizhou Rice Research Institute, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guizhou Rice Research Institute, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Plant Protection and Quarantine Station of Huangping County, Huangping 556100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanlie","family":"Yang","sequence":"additional","affiliation":[{"name":"Guizhou Rice Research Institute, Guiyang 550006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"8235","DOI":"10.1002\/ece3.6531","article-title":"Migration sources and pathways of the pest species Sogatella furcifera in Yunnan, China, and across the border inferred from DNA and wind analyses","volume":"10","author":"Hu","year":"2020","journal-title":"Ecol. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"104995","DOI":"10.1016\/j.pestbp.2021.104995","article-title":"A heat shock protein protects against oxidative stress induced by lambda-cyhalothrin in the green peach aphid Myzus persicae","volume":"181","author":"Dong","year":"2022","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/1743-422X-5-111","article-title":"Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection","volume":"5","author":"Callahan","year":"2008","journal-title":"Virol. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.physiol.61.1.243","article-title":"Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology","volume":"61","author":"Feder","year":"1999","journal-title":"Annu. Rev. Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1146\/annurev-ento-011613-162107","article-title":"Insect heat shock proteins during stress and diapause","volume":"60","author":"King","year":"2015","journal-title":"Annu. Rev. Entomol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Mack, L.K., and Attardo, G.M. (2024). Heat shock proteins, thermotolerance, and insecticide resistance in mosquitoes. Front. Insect Sci., 4.","DOI":"10.3389\/finsc.2024.1309941"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nrm3270","article-title":"The unfolded protein response: Controlling cell fate decisions under ER stress and beyond","volume":"13","author":"Hetz","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s12192-011-0285-3","article-title":"Reactivation of protein aggregates by mortalin and Tid1\u2014The human mitochondrial Hsp70 chaperone system","volume":"17","author":"Iosefson","year":"2012","journal-title":"Cell Stress Chaperon."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.pestbp.2016.08.002","article-title":"Effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant Laodelphax striatella","volume":"136","author":"Wang","year":"2017","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.pestbp.2017.01.011","article-title":"Characterization of heat shock protein 70 transcript from Nilaparvata lugens (St\u00e5l): Its response to temperature and insecticide stresses","volume":"142","author":"Lu","year":"2017","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.cbpa.2016.12.021","article-title":"Transcription of four Rhopalosiphum padi (L.) heat shock protein genes and their responses to heat stress and insecticide exposure","volume":"205","author":"Li","year":"2017","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s12192-019-01053-4","article-title":"Effects of abiotic stress on the expression of Hsp70 genes in Sogatella furcifera (Horv\u00e1th)","volume":"25","author":"Zhou","year":"2020","journal-title":"Cell Stress Chaperones"},{"key":"ref_13","first-page":"37","article-title":"Field efficacy of five commonly used insecticides against Nilaparvata lugens and Sogatella furcifera","volume":"37","author":"Zhang","year":"2025","journal-title":"Hubei Plant Prot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1127\/entomologia\/2022\/1496","article-title":"Role of insecticide-mediated transcription of the TOR and JH signaling pathway-related genesin the regulation ofreproduction in Sogatella furcifera","volume":"42","author":"Zhou","year":"2022","journal-title":"Entomol. Gen."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u2206\u2206CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1007\/s13744-023-01034-4","article-title":"Experimental Crosses Between Two Dung Beetle Lineages Show Transgressive Segregation in Physiological Traits","volume":"52","author":"Armas","year":"2023","journal-title":"Neotrop. Entomol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cois.2020.05.005","article-title":"Ontogenetic variation in thermal sensitivity shapes insect ecological responses to climate change","volume":"41","author":"Kingsolver","year":"2020","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"24","DOI":"10.4001\/003.022.0122","article-title":"Short-term increased expression of the heat shock protein 70 family members during heat shock is positively correlated with basal thermotolerance in the midgut of three strains of the silkworm, Bombyx Mori","volume":"22","author":"Lin","year":"2009","journal-title":"Afr. Entomol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.gene.2014.11.043","article-title":"Heat shock protein 70 gene family in the Glanville fritillary butterfly and their response to thermal stress","volume":"556","author":"Luo","year":"2015","journal-title":"Gene"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chen, X., Li, Z.D., Li, D.T., Jiang, M.X., and Zhang, C.X. (2021). HSP70\/DNAJ Family of Genes in the Brown Planthopper, Nilaparvata lugens: Diversity and Function. Genes, 12.","DOI":"10.3390\/genes12030394"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1002\/ps.7384","article-title":"Heat shock protein 70 and Cathepsin B genes are involved in the thermal tolerance of Aphis gossypii","volume":"79","author":"Liu","year":"2023","journal-title":"Pest Manag. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Liu, J., Tang, Y., Fang, Z., Wang, Z., Zhang, H., Guo, S., Lin, L., and Dang, W. (2026). Heat shock protein 70 in Cynops orientalis: Bidirectional thermal regulation and metabolic optimization in amphibian climatic adaptation. BMC Genom., 27.","DOI":"10.1186\/s12864-025-12501-0"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kleczewska, M., Grabinska, A., Jelen, M., Stolarska, M., Schilke, B., Marszalek, J., Craig, E.A., and Dutkiewicz, R. (2020). Biochemical Convergence of Mitochondrial Hsp70 System Specialized in Iron-Sulfur Cluster Biogenesis. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093326"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1002\/arch.20124","article-title":"Cloning and nucleotide sequencing of three heat shock protein genes (hsp90, hsc70, and hsp19.5) from the diamondback moth, Plutella xylostella (L.) and their expression in relation to developmental stage and temperature","volume":"62","author":"Sonoda","year":"2006","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s12192-022-01303-y","article-title":"Molecular characterization of six heat shock protein 70 genes from Arma chinensis and their expression patterns in response to temperature stress","volume":"27","author":"Meng","year":"2022","journal-title":"Cell Stress Chaperones"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Ma, W., Ma, C., Zhang, Q., Tian, Z., Tian, Z., Chen, H., Guo, J., Wan, F., and Zhou, Z. (2022). The hsp70 new functions as a regulator of reproduction both female and male in Ophraella communa. Front. Mol. Biosci., 9.","DOI":"10.3389\/fmolb.2022.931525"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Li, H., Li, S., Chen, J., Dai, L., Chen, R., Ye, J., and Hao, D. (2022). A heat shock 70kDa protein MaltHSP70-2 contributes to thermal resistance in Monochamus alternatus (Coleoptera: Cerambycidae): Quantification, localization, and functional analysis. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08858-1"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1002\/ps.70352","article-title":"MicroRNA Lse-miR-2944c-3p regulates female reproduction in Lasioderma serricorne by targeting the heat shock protein LsHSP70-3","volume":"82","author":"Xu","year":"2026","journal-title":"Pest Manag. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/jisesa\/iey059","article-title":"Comparisons of Expression Levels of Heat Shock Proteins (hsp70 and hsp90) From Anaphothrips obscurus (Thysanoptera: Thripidae) in Polymorphic Adults Exposed to Different Heat Shock Treatments","volume":"18","author":"Guo","year":"2018","journal-title":"J. Insect Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Jin, J., Zhao, M., Wang, Y., Zhou, Z., Wan, F., and Guo, J. (2020). Induced Thermotolerance and Expression of Three Key Hsp Genes (Hsp70, Hsp21, and sHsp21) and Their Roles in the High Temperature Tolerance of Agasicles hygrophila. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.01593"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Huang, Q., Tang, W., Liu, X., Ma, Q., Zhu-Salzman, K., and Cheng, W. (2025). Characterization of Two Novel Heat Shock Protein 70 Transcripts from Sitodiplosis mosellana and Their Response to Larval Diapause and Thermal Stress. Biology, 14.","DOI":"10.3390\/biology14091147"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2842","DOI":"10.1021\/acs.jafc.4c09505","article-title":"Characterizing Three Heat Shock Protein 70 Genes of Aphis gossypii and Their Expression in Response to Temperature and Insecticide Stress","volume":"73","author":"Liu","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"106827","DOI":"10.1016\/j.pestbp.2025.106827","article-title":"Functional analysis of heat shock protein 70 genes in Tetranychus truncatus reveals their critical role in cross-tolerance to heat and acaricide stress","volume":"216","author":"Sun","year":"2026","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Sannino, S., and Brodsky, J.L. (2017). Targeting protein quality control pathways in breast cancer. BMC Biol., 15.","DOI":"10.1186\/s12915-017-0449-4"}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/765\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:26:43Z","timestamp":1785162403000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/765"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["insects17080765"],"URL":"https:\/\/doi.org\/10.3390\/insects17080765","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}