{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:12:18Z","timestamp":1785409938257,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018818","name":"NKFIH","doi-asserted-by":"publisher","award":["K 134811"],"award-info":[{"award-number":["K 134811"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"award":["K 134811"],"award-info":[{"award-number":["K 134811"]}],"id":[{"id":"https:\/\/ror.org\/03g2am276","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Plant-mediated interactions can modify herbivore behaviour and vector-borne plant disease dynamics, yet the systemic consequences of prior cereal leaf beetle (Oulema spp.) window-pane feeding for later phloem feeder vectors remain unclear. We hypothesised that prior Oulema window-pane feeding induces systemic changes in barley that alter the subsequent host acceptance and feeding behaviour of Psammotettix alienus. Plants received one of three 24 h treatments on one leaf: Oulema pre-colonisation, standardised artificial vascular injury or an undamaged control. Leafhopper responses were measured on a different, systemic leaf using female choice assays and electrical penetration graph recordings. Oulema damage was low and strongly right-skewed and did not affect most feeding variables, including non-phloem activities, phloem activity, drinking latency or phloem activity latency. Artificial vascular injury, however, reduced the phloem\u2013saliva ratio in females, which indicates a lower relative investment in phloem preparation once the phloem phase was reached. Within Oulema-treated plants, increasing damage reduced the number of transitions between penetration phases in males but not females. Females showed no treatment-related preference in salivary sheath production or oviposition. Thus, Oulema window-pane feeding left only a weak systemic plant-mediated legacy for P. alienus, closer to behavioural neutrality than strong facilitation or interference under these short-term systemic experimental conditions.<\/jats:p>","DOI":"10.3390\/insects17080783","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:57:28Z","timestamp":1785254248000},"page":"783","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prior Cereal Leaf Beetle (Oulema spp.) Window-Pane Feeding Has Weak Systemic Legacy on the Wheat Dwarf Virus Vector Psammotettix alienus in Barley"],"prefix":"10.3390","volume":"17","author":[{"given":"Regina","family":"Gerstenbrand","sequence":"first","affiliation":[{"name":"HUN-REN Centre for Agricultural Research, Plant Protection Institute, 1518 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"HUN-REN Centre for Agricultural Research, Plant Protection Institute, 1518 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6591-2284","authenticated-orcid":false,"given":"Ferenc","family":"Samu","sequence":"additional","affiliation":[{"name":"HUN-REN Centre for Agricultural Research, Plant Protection Institute, 1518 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3364-0322","authenticated-orcid":false,"given":"Gergely","family":"Tholt","sequence":"additional","affiliation":[{"name":"HUN-REN Centre for Agricultural Research, Plant Protection Institute, 1518 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1086\/284302","article-title":"Evolution of Insect Host Plant Relationships","volume":"124","author":"Jermy","year":"1984","journal-title":"Am. 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