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However, the oriental fruit fly has gradually developed resistance to multiple insecticides, posing significant challenges for pest resistance management. This study investigated changes in insecticide resistance in oriental fruit flies under different selection pressures. We identified resistance-related genes and their mutation sites using cDNA probes. Our results showed that resistance to trichlorfon decreased significantly in the absence of selection pressure. However, resistance increased when selection pressures were applied every 30 or 60 days. Although the resistance ratio showed a decreasing trend at every 90 and 120 days, a risk of a rebound remained. A large number of homologous sequences were identified in the suppression subtractive hybridization library of insecticide-resistant strains. Resistance to trichlorfon and alphamethrin primarily involves detoxification and metabolic genes, which are often associated with cross-resistance. A single gene may detoxify multiple pesticides, leading to cross-resistance. The four acetylcholinesterase mutations identified in this study are consistent with previous research findings. Moreover, resistance results from the combined action of multiple genes. Based on these findings, this study recommends avoiding continuous use of trichlorfon and maintaining an interval of more than 80 days between applications. It also emphasizes the importance of incorporating pesticide application intervals into integrated pest management strategies. This study provides a theoretical foundation for delaying resistance development in the oriental fruit fly, offers guidance on proper pesticide application intervals in the field, and contributes practical insights for pest resistance management.<\/jats:p>","DOI":"10.3390\/insects17080784","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T10:13:47Z","timestamp":1785320027000},"page":"784","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insecticide Resistance Dynamics and Gene Screening in Bactrocera dorsalis (Hendel) Under Varying Selection Pressures"],"prefix":"10.3390","volume":"17","author":[{"given":"Doudou","family":"Li","sequence":"first","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2094-7280","authenticated-orcid":false,"given":"Qi","family":"Yao","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Hainan Academy of Agricultural Sciences, Haikou 570100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Agricultural Engineering, Guangxi Vocational University of Agriculture, Nanning 530003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daifeng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangwen","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongyue","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.4238\/2012.July.10.12","article-title":"Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae","volume":"11","author":"Jin","year":"2012","journal-title":"Genet. Mol. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S2095-3119(18)62015-5","article-title":"Invasion, expansion and control of Bactrocera dorsalis (Hendel) in China","volume":"18","author":"Liu","year":"2019","journal-title":"J. Integr. Agric."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105498","DOI":"10.1016\/j.pestbp.2023.105498","article-title":"Functional analysis of an overexpressed glutathione S-transferase BdGSTd5 involved in malathion and malaoxon detoxification in Bactrocera dorsalis","volume":"194","author":"Zhang","year":"2023","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1127\/entomologia\/2024\/2711","article-title":"Advancements in Integrated Pest Management strategies for Bactrocera dorsalis in Asia: Current status, insights, and future prospects","volume":"44","author":"Wang","year":"2024","journal-title":"Entomol. Gen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1002\/ps.2076","article-title":"Insecticide resistance of the oriental fruit fly (Bactrocera dorsalis (Hendel)) (Diptera: Tephritidae) in mainland China","volume":"67","author":"Jin","year":"2011","journal-title":"Pest Manag. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Li, X., Li, P., Li, D., Cai, X., Gu, S., Zeng, L., Cheng, D., and Lu, Y. (2024). Dynamics of Bactrocera dorsalis Resistance to Seven Insecticides in South China. Insects, 15.","DOI":"10.3390\/insects15090679"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Li, D., Cai, X., Qi, Y., Lu, Y., and Li, X. (2024). Lethal, Sublethal, and Offspring Effects of Fluralaner and Dinotefuran on Three Species of Bactrocera Fruit Flies. Insects, 15.","DOI":"10.3390\/insects15060440"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pestbp.2009.07.013","article-title":"Identification of resistance-responsive proteins in larvae of Bactrocera dorsalis (Hendel), for pyrethroid toxicity by a proteomic approach","volume":"96","author":"Jin","year":"2010","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.pestbp.2011.02.005","article-title":"Molecular and biochemical mechanisms of organophosphate resistance in laboratory-selected lines of the oriental fruit fly (Bactrocera dorsalis)","volume":"100","author":"Hsu","year":"2011","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1603\/EC11200","article-title":"Fitness Costs of an Insecticide Resistance and Their Population Dynamical Consequences in the Oriental Fruit Fly","volume":"104","author":"Fang","year":"2011","journal-title":"J. Econ. Entomol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.pestbp.2016.02.001","article-title":"Identification of a carboxylesterase associated with resistance to naled in Bactrocera dorsalis (Hendel)","volume":"131","author":"Hsu","year":"2016","journal-title":"Pest. Biochem. Phys."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Hafeez, M., Li, X.W., Ullah, F., Zhang, Z.J., Zhang, J.M., Huang, J., Chen, L.M., Siddiqui, J.A., Ren, X.Y., and Zhou, S.X. (2022). Characterization of indoxacarb resistance in the fall armyworm: Selection, inheritance, cross-resistance, possible biochemical mechanisms, and fitness costs. Biology, 11.","DOI":"10.3390\/biology11121718"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8400","DOI":"10.1021\/acs.jafc.3c01563","article-title":"An Antenna-Abundant Glutathione S-Transferase BdGSTd8 Participates in Detoxification of Two Organophosphorus Insecticides in Bactrocera dorsalis (Hendel)","volume":"71","author":"Meng","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Li, D., Chen, L., Cai, X., Qi, Y., and Lu, Y. (2024). Comparative Population Biology and Related Gene Expression in the Beta-Cypermethrin-Resistant Strains of Bactrocera dorsalis (Hendel). Insects, 15.","DOI":"10.3390\/insects15080569"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/jee\/tov276","article-title":"Inheritance, Realized Heritability, and Biochemical Mechanisms of Malathion Resistance in Bactrocera dorsalis (Diptera: Tephritidae)","volume":"109","author":"Wang","year":"2016","journal-title":"J. Econ. Entomol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ibmb.2014.03.012","article-title":"Molecular biology of insect sodium channels and pyrethroid resistance","volume":"50","author":"Dong","year":"2014","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_17","first-page":"1","article-title":"Effects of biological and environmental factors on the emergence and development of pest resistance","volume":"5","author":"Li","year":"2023","journal-title":"Plant Prot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"wrae058","DOI":"10.1093\/ismejo\/wrae058","article-title":"Lactic acid bacteria modulate the CncC pathway to enhance resistance to \u03b2-alphamethrin in the oriental fruit fly","volume":"18","author":"Zeng","year":"2024","journal-title":"ISME J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1002\/ps.6039","article-title":"Insecticide rotation scheme restores insecticide susceptibility in thiamethoxam-resistant field populations of Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), in Florida","volume":"77","author":"Chen","year":"2021","journal-title":"Pest Manag. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1111\/j.1439-0418.2008.01373.x","article-title":"Organophosphosphate resistance-related mutations in the acetylcholinesterase gene of Tephritidae","volume":"132","author":"Kakani","year":"2008","journal-title":"J. Appl. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Paulose, B., Kandasamy, S., and Dhankher, O.P. (2010). Expression profiling of Crambe abyssinica under arsenate stress identifies genes and gene networks involved in arsenic metabolism and detoxification. BMC Plant Biol., 10.","DOI":"10.1186\/1471-2229-10-108"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1182\/blood.V118.21.3195.3195","article-title":"Expressions of KLF1, FOXO3a, HOOK3 and MIER1 genes Are Associated with Fetal Hemoglobin Levels in Hereditary Persistence of Fetal Hemoglobin","volume":"118","author":"Roversi","year":"2011","journal-title":"Blood"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2932","DOI":"10.1002\/ps.5891","article-title":"Genome-wide and expression-profiling analyses of the cytochrome P450 genes in Bactrocera dorsalis (Hendel) and screening of candidate P450 genes associated with malathion resistance","volume":"76","author":"Jing","year":"2020","journal-title":"Pest Manag. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.aspen.2021.11.001","article-title":"SSH reveals different functional categories of putative resin synthesizing genes in the industrial insect, Kerria lacca (Kerr)","volume":"24","author":"Thamilarasi","year":"2021","journal-title":"J. Asia-Pac. Entomol."},{"key":"ref_25","first-page":"437","article-title":"Oligonucleotide immobilization using 10-(carbomethoxy)decyl-dimethylchlorosilane for mRNA isolation and cDNA synthesis on a microfluidic chip","volume":"155","author":"Banda","year":"2010","journal-title":"Sens. Actuators B Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/emm.2005.46","article-title":"Development of an angiogenesis-focused cDNA chip and validation of its functionality","volume":"37","author":"Shim","year":"2005","journal-title":"Exp. Mol. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1093\/nar\/27.6.1517","article-title":"Combining SSH and cDNA microarrays for rapid identification of differentially expressed genes","volume":"27","author":"Yang","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/s00425-007-0502-6","article-title":"Differentially expressed cDNAs at the early stage of banana ripening identified by suppression subtractive hybridization and cDNA microarray","volume":"226","author":"Xu","year":"2007","journal-title":"Planta"},{"key":"ref_29","first-page":"24","article-title":"Combining suppressive subtractive hybridization and cDNA microarrays to identify dietary phosphorus-responsive genes of the rainbow trout (Oncorhynchus mykiss) kidney","volume":"5","author":"Lake","year":"2010","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1073\/pnas.90.5.1957","article-title":"A single-amino acid substitution in a gamma-aminobutyric acid subtype A receptor locus is associated with cyclodiene insecticide resistance in Drosophila populations","volume":"90","author":"Constant","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1093\/molbev\/msr294","article-title":"High Resolution Mapping of Candidate Alleles for Desiccation Resistance in Drosophila melanogaster under Selection","volume":"29","author":"Marina","year":"2012","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.pestbp.2012.05.002","article-title":"Cuticular penetration of \u03b2-cypermethrin in insecticide-susceptible and resistant strains of Bactrocera dorsalis","volume":"103","author":"Lin","year":"2012","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Nebangwa, T., Mugenzi, J., Muhammad, A., Nebangwa, N., Kouamo, M., Tagne, D., Tekoh, A., Tchouakui, M., Ghogomu, M., and Ibrahim, S. (2024). Two highly selected mutations in the tandemly duplicated CYP6P4a and CYP6P4b genes drive pyrethroid resistance in Anopheles funestus in West Africa. BMC Biol., 22.","DOI":"10.1186\/s12915-024-02081-y"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"R27","DOI":"10.1186\/gb-2014-15-2-r27","article-title":"A single mutation in the GSTe2 gene allows tracking of metabolically based insecticide resistance in a major malaria vector","volume":"15","author":"Riveron","year":"2014","journal-title":"Genome Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1127\/entomologia\/2024\/2654","article-title":"A novel potential nAChR variation and the upregulation of CYP304A1 contribute to resistance against lambda-cyhalothrin and spinosad in Bactrocera dorsalis","volume":"44","author":"Liu","year":"2024","journal-title":"Entomol. Gen."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"105972","DOI":"10.1016\/j.pestbp.2024.105972","article-title":"GABA-gated chloride channel mutation (Rdl) induces cholinergic physiological compensation resulting in cross resistance in Drosophila melanogaster","volume":"203","author":"Xie","year":"2014","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_37","first-page":"22554","article-title":"N318L Blocks the Interaction of Fluralaner but Not Broflanilide or Fipronil with the Insect GABA Receptor In Vivo","volume":"72","author":"Zhang","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Huang, Q., Sheng, C., Liu, G., Zhang, K., Jia, Z., Tang, T., Mao, X., Jones, A.K., and Han, Z. (2023). G3\u2019MTMD3 in the insect GABA receptor subunit, RDL, confers resistance to broflanilide and fluralaner. PLoS Genet., 19.","DOI":"10.1371\/journal.pgen.1010814"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103934","DOI":"10.1016\/j.ibmb.2023.103934","article-title":"A single point mutation in the Bemisia tabaci cytochrome-P450 CYP6CM1 causes enhanced resistance to neonicotinoids","volume":"156","author":"Pym","year":"2023","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1002\/ps.4798","article-title":"Novel cytochrome P450 (CYP6D1) and voltage sensitive sodium channel (Vssc) alleles of the house fly (Musca domestica) and their roles in pyrethroid resistance","volume":"74","author":"Pan","year":"2018","journal-title":"Pest Manag. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3469","DOI":"10.1534\/g3.118.200537","article-title":"Signatures of Insecticide Selection in the Genome of Drosophila melanogaster","volume":"8","author":"Duneau","year":"2018","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Hu, B., Zhang, Y., Xing, Z., Chen, X., Rao, C., Liu, K., Tan, A., and Su, J. (2025). Two independent regulatory mechanisms synergistically contribute to P450-mediated insecticide resistance in a lepidopteran pest, Spodoptera exigua. BMC Biol., 23.","DOI":"10.1186\/s12915-025-02228-5"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.chemosphere.2018.02.135","article-title":"Benzene induced resistance in exposed Drosophila melanogaster: Outcome of improved detoxification and gene modulation","volume":"201","author":"Sharma","year":"2018","journal-title":"Chemosphere"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Lu, W., Liu, Z., Fan, X., Zhang, X., Qiao, X., and Huang, J. (2022). Nicotinic acetylcholine receptor modulator insecticides act on diverse receptor subtypes with distinct subunit compositions. PLoS Genet., 18.","DOI":"10.1371\/journal.pgen.1009920"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"106702","DOI":"10.1016\/j.pestbp.2025.106702","article-title":"Target identification and mechanism of action of the neonicotinoid Cycloxaprid","volume":"215","author":"Qiao","year":"2025","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Menozzi, P., Shi, M.A., Lougarre, A., Zhen Tang, H., and Fournier, D. (2004). Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations. BMC Evol. Biol., 4.","DOI":"10.1186\/1471-2148-4-4"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5741","DOI":"10.1002\/ps.8304","article-title":"Monitoring of the nAChRs\u03b16 G275A spinetoram resistance allele in Drosophila melanogaster populations from New York vineyards","volume":"80","author":"Scott","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Yin, C., O\u2019Reilly, A.O., Liu, S.N., Du, T.H., Gong, P.P., Zhang, C.J., Wei, X.G., Yang, J., Huang, M.J., and Fu, B.L. (2024). Dual mutations in the whitefly nicotinic acetylcholine receptor \u03b21 subunit confer target-site resistance to multiple neonicotinoid insecticides. PLoS Genet., 20.","DOI":"10.1371\/journal.pgen.1011163"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e21523","DOI":"10.1002\/arch.21523","article-title":"Protein-protein interaction network analysis of insecticide resistance molecular mechanism in Drosophila melanogaster","volume":"100","author":"Zhang","year":"2019","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"102105","DOI":"10.1016\/j.aspen.2023.102105","article-title":"CRISPR\/Cas9 editing of transformer2 gene of the Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) leads to intersex phenotype","volume":"26","author":"Wishard","year":"2023","journal-title":"J. Asia-Pac. Entomol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1038\/s42003-024-06505-x","article-title":"AANAT1 regulates insect midgut detoxification through the ROS\/CncC pathway","volume":"7","author":"Zeng","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_52","first-page":"895935","article-title":"Life-History Traits and Population Relative Fitness of Trichlorphon-Resistant and -Susceptible Bactrocera dorsalis (Diptera: Tephritidae)","volume":"2010","author":"Zhang","year":"2010","journal-title":"Psyche J. Entomol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s13355-013-0232-0","article-title":"A novel point mutation of acetylcholinesterase in a trichlorfon-resistant strain of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)","volume":"49","author":"Jiang","year":"2014","journal-title":"Appl. Entomol. Zool."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/117248a0","article-title":"The Control of Insect Pests in Agriculture","volume":"117","author":"Imms","year":"2010","journal-title":"Nature"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.pestbp.2011.04.004","article-title":"Insecticide resistance in Tephritid flies","volume":"100","author":"Vontas","year":"2011","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Wan, X.W., Liu, Y.H., and Zhang, B. (2012). Invasion History of the Oriental Fruit Fly, Bactrocera dorsalis, in the Pacific-Asia Region: Two Main Invasion Routes. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036176"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1111\/imb.12161","article-title":"Discovery of genes related to formothion resistance in oriental fruit fly (Bactrocera dorsalis) by a constrained functional genomics analysis","volume":"24","author":"Kuo","year":"2015","journal-title":"Insect Mol. Biol."},{"key":"ref_58","first-page":"791","article-title":"Dynamic of resistance of Bactrocera dorsalis (Hendel) high-resistant strain under selection by beta-cypermethrin with different frequency","volume":"39","author":"Yao","year":"2017","journal-title":"J. Environ. Entomol."},{"key":"ref_59","first-page":"864","article-title":"A comparative study of the population biology of trichlorfon-resistant strains of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae)","volume":"58","author":"Chen","year":"2015","journal-title":"Acta Entomol. Sin."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13071-022-05175-0","article-title":"CPR63 promotes pyrethroid resistance by increasing cuticle thickness in Culex pipiens pallens","volume":"15","author":"Xu","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jare.2022.12.012","article-title":"Genomes of the cosmopolitan fruit pest Bactrocera dorsalis (Diptera: Tephritidae) reveal its global invasion history and thermal adaptation","volume":"53","author":"Zhang","year":"2023","journal-title":"J. Adv. Res."},{"key":"ref_62","first-page":"978","article-title":"Effects of four plant secondary compounds on the resistance to the trichlorphon-resistant strains of Bactrocera dorsalis (Hendel)","volume":"46","author":"Gao","year":"2024","journal-title":"J. Environ. Entomol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1146\/annurev-ento-121123-021252","article-title":"Insect Pest Management and Environmental Risk","volume":"70","author":"Peterson","year":"2025","journal-title":"Annu. Rev. Entomol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1002\/ps.5318","article-title":"Two delta class glutathione S-transferases involved in the detoxification of malathion in Bactrocera dorsalis (Hendel)","volume":"75","author":"Meng","year":"2019","journal-title":"Pest Manag. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"126836","DOI":"10.1016\/j.ijbiomac.2023.126836","article-title":"Characterization and transcriptional expression of ABCG genes in Bactrocera dorsalis: Insights into their roles in fecundity and insecticidal stress response","volume":"253","author":"Wang","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Zeng, W., Sun, Z., Lai, Z., Yang, S., Chen, H., Yang, X., Tao, J., and Tang, X. (2019). Determination of the MiRNAs Related to Bean Pyralid Larvae Resistance in Soybean Using Small RNA and Transcriptome Sequencing. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20122966"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"107793","DOI":"10.1016\/j.soilbio.2020.107793","article-title":"Comparing high throughput sequencing and real time qPCR for characterizing entomopathogenic nematode biogeography","volume":"145","author":"Dritsoulas","year":"2020","journal-title":"Soil Biol. Biochem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.ijbiomac.2018.02.085","article-title":"De novo transcriptome assembly and analysis of differential gene expression following peptidoglycan (PGN) challenge in Antheraea pernyi","volume":"112","author":"Liu","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.cois.2018.04.005","article-title":"Copy number variation (CNV) and insecticide resistance in mosquitoes: Evolving knowledge or an evolving problem?","volume":"27","author":"Weetman","year":"2018","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s40643-022-00515-5","article-title":"Degradation insight of organophosphate pesticide chlorpyrifos through novel intermediate 2,6-dihydroxypyridine by Arthrobacter sp. HM01","volume":"9","author":"Mali","year":"2022","journal-title":"Bioresour. Bioprocess."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e2413587122","DOI":"10.1073\/pnas.2413587122","article-title":"Copy number variation contributes to parallel local adaptation in an invasive plant","volume":"122","author":"Wilson","year":"2025","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ibmb.2006.10.001","article-title":"A new amino-acid substitution in acetylcholinesterase 1 confers insecticide resistance to Culex pipiens mosquitoes from Cyprus","volume":"37","author":"Alout","year":"2007","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1002\/ps.8003","article-title":"Acetylcholine esterase of Drosophila melanogaster: A laboratory model to explore insecticide susceptibility gene drives","volume":"80","author":"Hernandes","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"eadh3425","DOI":"10.1126\/sciadv.adh3425","article-title":"From sub-Saharan Africa to China: Evolutionary history and adaptation of Drosophila melanogaster revealed by population genomics","volume":"10","author":"Chen","year":"2024","journal-title":"Sci. Adv."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"9961","DOI":"10.1038\/s41467-024-54210-4","article-title":"A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population","volume":"15","author":"Auradkar","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"118189","DOI":"10.1016\/j.ecoenv.2025.118189","article-title":"New mutations of acetylcholinesterase in Bactrocera dorsalis populations across southern China and impact on resistance to organophosphate","volume":"297","author":"Fan","year":"2025","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_77","first-page":"1075","article-title":"Cross-resistance of Bactrocera dorsalis (Diptera: Tephritidae) to beta-cypermethrin, trichlorfon and abamectin","volume":"67","author":"Li","year":"2024","journal-title":"Acta Entomol. Sin."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Kouam\u00e9, J.K.I., Edi, C.V.A., Zahouli, J.B.Z., Kouam\u00e9, R.M.A., Kacou, Y.A.K., Yokoly, F.N., Gbalegba, C.G.N., Malone, D., and Koudou, B.G. (2024). Assessing species composition and insecticide resistance of Anopheles gambiae complex members in three coastal health districts of C\u00f4te d\u2019Ivoire. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0297604"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"106481","DOI":"10.1016\/j.mimet.2022.106481","article-title":"Pulling needles out of a haystack: Subtractive Community Metatranscriptomics retrieves anaerobic o-xylene degradation pathway genes out of a mixed microbial culture","volume":"197","author":"Rossmassler","year":"2022","journal-title":"J. Microbiol. Methods"},{"key":"ref_80","first-page":"862","article-title":"The roles of CPR and P450 genes in the resistance of insects to insecticides","volume":"45","author":"Qiao","year":"2023","journal-title":"J. Environ. Entomol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1002\/ps.7939","article-title":"What is the value of rotations to insecticide resistance management?","volume":"80","author":"Madgwick","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Shikano, I., Gutierrez-Coarite, R., Streit, C., Perez, E., Fujitani, E., and Mau, R.F.L. (2022). Field tests of three alternative insecticides with protein bait for the development of an insecticide rotation program to control melon flies, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae). Insects, 13.","DOI":"10.3390\/insects13070629"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1002\/ps.6254","article-title":"Insecticide resistance management and industry: The origins and evolution of the Insecticide Resistance Action Committee (IRAC) and the mode of action classification scheme","volume":"77","author":"Sparks","year":"2021","journal-title":"Pest Manag. Sci."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/784\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T04:30:28Z","timestamp":1785472228000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":83,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["insects17080784"],"URL":"https:\/\/doi.org\/10.3390\/insects17080784","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]}}}