{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:45:51Z","timestamp":1785818751713,"version":"3.56.0"},"reference-count":67,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"INTA","award":["2023-PD-L06-I116"],"award-info":[{"award-number":["2023-PD-L06-I116"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Microbial communities associated with insect larvae play key roles in host nutrition, protection against pathogens, immune modulation, and represent a source of biotechnologically relevant traits. In Alphitobius diaperinus, a major poultry pest, the gut microbiota has been mainly studied at the community level, providing limited insight into the potential roles of individual microorganisms. Here, we characterized the aerobically culturable fraction of the larval gut microbiota derived from a single pooled homogenate of fifteen larvae under stable laboratory rearing conditions\u2014an approach that uniquely enables strain-level genomic resolution, functional experimentation, and biotechnological exploitation. Ten bacterial strains and two yeasts were initially isolated; eight bacterial isolates were identical, revealing the most frequently recovered cultivable bacterial morphotype. We provide high-quality draft genomes for five members of the A. diaperinus cultivable gut microbiota\u2014Enterobacter hormaechei subsp. xiangfangensis strain INTA AN1-1, Staphylococcus hominis subsp. novobiosepticus strain INTA AC1-4, Staphylococcus succinus subsp. succinus strain INTA AC1-8, Hyphopichia burtonii strain INTA AB1-1, and Debaryomyces fabryi strain INTA AB1-4\u2014establishing a genome-resolved reference framework validated through a polyphasic approach. Genome-scale annotation enabled us to predict the metabolic potential and hypothesize the functional capabilities among cultivable members, providing a foundation for future studies on this economically relevant pest.<\/jats:p>","DOI":"10.3390\/insects17080800","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:51:23Z","timestamp":1785772283000},"page":"800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Characterization of Aerobically Culturable Gut-Associated Bacteria and Yeasts Isolated from Pooled Larval Midguts of the Lesser Mealworm Alphitobius diaperinus (Coleoptera: Tenebrionidae)"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0291-5303","authenticated-orcid":false,"given":"Gisele Ivonne","family":"Antonuccio","sequence":"first","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Instituto de Microbiolog\u00eda y Zoolog\u00eda Agr\u00edcola (IMYZA), Buenos Aires 1686, Argentina"},{"name":"Comisi\u00f3n de Investigaciones Cient\u00edficas (CIC), Gobierno de la Provincia de Buenos Aires, La Plata 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo Juli\u00e1n","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Instituto de Microbiolog\u00eda y Zoolog\u00eda Agr\u00edcola (IMYZA), Buenos Aires 1686, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6065-4626","authenticated-orcid":false,"given":"Marcelo Facundo","family":"Berretta","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Instituto de Microbiolog\u00eda y Zoolog\u00eda Agr\u00edcola (IMYZA), Buenos Aires 1686, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires 1425, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3866-7180","authenticated-orcid":false,"given":"Diego Herman","family":"Sauka","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria (INTA), Instituto de Microbiolog\u00eda y Zoolog\u00eda Agr\u00edcola (IMYZA), Buenos Aires 1686, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires 1425, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,2]]},"reference":[{"key":"ref_1","first-page":"100110","article-title":"A comprehensive account of functional role of insect gut microbiome in insect orders","volume":"11","author":"Yasika","year":"2025","journal-title":"J. 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