{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:48:26Z","timestamp":1785775706775,"version":"3.56.0"},"reference-count":53,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2023YFE0207400"],"award-info":[{"award-number":["2023YFE0207400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["6242020"],"award-info":[{"award-number":["6242020"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Bactrocera dorsalis (Diptera: Tephritidae) ranks among the most serious quarantine pests threatening global fruit and crop production. Irradiation has been adopted worldwide as an eco-friendly phytosanitary treatment strategy, yet how B. dorsalis responds to irradiation is not fully understood. To investigate the transcriptional response to irradiation, third-instar B. dorsalis larvae were exposed to two X-ray doses: a sub-effective dose of 15 Gy and a phytosanitary dose of 120 Gy. Comparative transcriptomic profiling revealed a clear dose-dependent escalation in the number of differentially expressed gene (DEGs). While 15 Gy primarily disrupted basic metabolism and cuticle integrity, 120 Gy induced a systemic metabolic collapse coupled with severe genotoxic stress, activating DNA repair, lysosomal degradation, and the JAK-STAT signaling pathway. Notably, we identified nine dose-specific candidate biomarker genes that are exclusively regulated at the phytosanitary dose. These findings elucidate the molecular network mediating radiation-induced mortality and provide rapid molecular diagnostic tools for validating phytosanitary irradiation efficacy and optimizing pest management strategies.<\/jats:p>","DOI":"10.3390\/insects17080804","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:51:23Z","timestamp":1785772283000},"page":"804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Comparative Transcriptomic Analysis of X-Ray Irradiation Responses Identifies Candidate Biomarker Genes in Bactrocera dorsalis Larvae"],"prefix":"10.3390","volume":"17","author":[{"given":"Baishu","family":"Li","sequence":"first","affiliation":[{"name":"Chinese Academy of Quality and Inspection & Testing, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sihan","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haomiao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Quality and Inspection & Testing, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-151X","authenticated-orcid":false,"given":"Junzheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4111-7961","authenticated-orcid":false,"given":"Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Quality and Inspection & Testing, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev.ento.50.071803.130428","article-title":"Invasive phytophagous pests arising through a recent tropical evolutionary radiation: The Bactrocera dorsalis complex of fruit flies","volume":"50","author":"Clarke","year":"2005","journal-title":"Annu. 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