{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T04:31:38Z","timestamp":1786509098579,"version":"build-2736575974"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2014Brasil","doi-asserted-by":"publisher","award":["306207\/2023-0"],"award-info":[{"award-number":["306207\/2023-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["306207\/2023-0"],"award-info":[{"award-number":["306207\/2023-0"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Spodoptera frugiperda (Lepidoptera: Noctuidae) is a key insect pest of maize in Brazil. However, the evolution of insecticide resistance has raised concerns about the risk of control failures under field conditions. This study assessed insecticide efficacy and estimated the risk of control failure in Brazilian populations of S. frugiperda exposed to selected insecticides. Nine field populations were collected from representative maize-producing regions of Brazil during the 2024\/2025 and 2025\/2026 seasons. Early third-instar (L3) larvae from these populations were exposed to the highest label rate of ten insecticides representing different modes of action in maize leaf bioassays. Mortality was assessed 96 h after exposure, and the risk of control failure was based on the efficacy threshold of 80% mortality. Significant effects of population, insecticide, and the population \u00d7 insecticide interaction were detected, indicating variation in susceptibility among the sampled populations. Methoxyfenozide + spinetoram, spinosad, chlorfenapyr, and spinetoram consistently caused high mortality (&gt;81%) and were associated with low risk of control failure across all populations. In contrast, chlorantraniliprole exhibited reduced efficacy with mortality below the expected efficacy threshold in most populations and a high risk of control failure. Population-dependent variation in insecticide efficacy was also evident for metaflumizone, emamectin benzoate, thiodicarb, indoxacarb, and methoxyfenozide, with corresponding variation in the risk of control failure. These findings show that insecticide efficacy and the risk of control failure depend on the specific insecticide\u2013population combination and provide valuable information to support decision-making regarding insecticide selection and the rotation of modes of action within insect resistance management (IRM) and integrated pest management (IPM) programs.<\/jats:p>","DOI":"10.3390\/insects17080825","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:23:50Z","timestamp":1786357430000},"page":"825","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Variation in Label-Rate Insecticide Efficacy and Control Failure Risk Among Brazilian Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9790-7738","authenticated-orcid":false,"given":"Zanandra Z.","family":"Tamiosso","sequence":"first","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2849-8886","authenticated-orcid":false,"given":"Daniela N.","family":"Godoy","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4620-3762","authenticated-orcid":false,"given":"Ramon B.","family":"Palharini","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica L. S.","family":"Grzybowski","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1980-6816","authenticated-orcid":false,"given":"Marylia P.","family":"Cargnin","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9201-0163","authenticated-orcid":false,"given":"Venicius E.","family":"Pretto","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6749-952X","authenticated-orcid":false,"given":"Arthur","family":"Dallanora","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josemar","family":"Foresti","sequence":"additional","affiliation":[{"name":"Corteva Agriscience, Barueri 06454-080, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionei S.","family":"Muraro","sequence":"additional","affiliation":[{"name":"Corteva Agriscience, Barueri 06454-080, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo R. da","family":"Silva","sequence":"additional","affiliation":[{"name":"Corteva Agriscience, Barueri 06454-080, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9320-1642","authenticated-orcid":false,"given":"Oderlei","family":"Bernardi","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, Federal University of Santa Maria (UFSM), Roraima Avenue 1000, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1127\/entomologia\/2022\/1659","article-title":"Invasiveness, biology, ecology, and management of the fall armyworm, Spodoptera frugiperda","volume":"43","author":"Kenis","year":"2022","journal-title":"Entomol. Gen."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"286","DOI":"10.4001\/003.026.0286","article-title":"Host plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas","volume":"26","author":"Montezano","year":"2018","journal-title":"Afr. Entomol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1570-7458.2010.01058.x","article-title":"Development of Spodoptera frugiperda on different hosts and damage to reproductive structures in cotton","volume":"137","author":"Barros","year":"2010","journal-title":"Entomol. Exp. Appl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"10080","DOI":"10.1038\/s41598-020-67339-1","article-title":"Cross-crop resistance of Spodoptera frugiperda selected on Bt maize to genetically modified soybean expressing Cry1Ac and Cry1F proteins in Brazil","volume":"10","author":"Machado","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/jipm\/pmx011","article-title":"Adaptive potential of fall armyworm (Lepidoptera: Noctuidae) limits Bt trait durability in Brazil","volume":"8","author":"Fatoretto","year":"2017","journal-title":"J. Integr. Pest Manag."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1093\/jee\/toab011","article-title":"Baseline response, monitoring, and cross-resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to sodium channel blocker insecticides in Brazil","volume":"114","author":"Kaiser","year":"2021","journal-title":"J. Econ. Entomol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.cropro.2014.06.019","article-title":"Field-evolved resistance to Cry1F maize by Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil","volume":"64","author":"Farias","year":"2014","journal-title":"Crop Prot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1002\/ps.4201","article-title":"Field-evolved resistance to Cry1Ab maize by Spodoptera frugiperda in Brazil","volume":"72","author":"Omoto","year":"2016","journal-title":"Pest Manag. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1093\/jee\/toac183","article-title":"Global patterns of insect resistance to transgenic Bt crops: The first 25 years","volume":"116","author":"Tabashnik","year":"2023","journal-title":"J. Econ. Entomol."},{"key":"ref_10","first-page":"1","article-title":"Primer registro de supervivencia de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) en ma\u00edz Bt que expresa la prote\u00edna Vip3Aa20, en la Argentina","volume":"103","author":"Vera","year":"2026","journal-title":"Rev. Ind. Agric. Tucum\u00e1n"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1002\/ps.4660","article-title":"Managing fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), with Bt maize and insecticides in southern Brazil","volume":"73","author":"Burtet","year":"2017","journal-title":"Pest Manag. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1093\/jee\/toac108","article-title":"Chemical control and insecticide resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae)","volume":"115","year":"2022","journal-title":"J. Econ. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Paredes-S\u00e1nchez, F.A., Rivera, G., Bocanegra-Garc\u00eda, V., Mart\u00ednez-Padr\u00f3n, H.Y., Berrones-Morales, M., Ni\u00f1o-Garc\u00eda, N., and Herrera-Mayorga, V. (2021). Advances in control strategies against Spodoptera frugiperda: A review. Molecules, 26.","DOI":"10.3390\/molecules26185587"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1007\/s41348-023-00777-x","article-title":"The invasive fall armyworm, Spodoptera frugiperda, in Africa and Asia: Responding to the food security challenge, with priorities for integrated pest management research","volume":"130","author":"Mendesil","year":"2023","journal-title":"J. Plant Dis. Prot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1590\/S1519-566X2001000200016","article-title":"Heran\u00e7a da resist\u00eancia de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) a lambda-cialotrina","volume":"30","author":"Omoto","year":"2001","journal-title":"Neotrop. Entomol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Carvalho, R.A., Omoto, C., Field, L.M., Williamson, M.S., and Bass, C. (2013). Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062268"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1002\/ps.4057","article-title":"Genetic basis of Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance to the chitin-synthesis inhibitor lufenuron","volume":"72","author":"Farias","year":"2016","journal-title":"Pest Manag. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1002\/ps.4829","article-title":"Inheritance and fitness costs of Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance to spinosad in Brazil","volume":"74","author":"Okuma","year":"2018","journal-title":"Pest Manag. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2682","DOI":"10.1002\/ps.5376","article-title":"Selection and characterization of the inheritance of resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to chlorantraniliprole and cross-resistance to other diamide insecticides","volume":"75","author":"Bolzan","year":"2019","journal-title":"Pest Manag. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"106071","DOI":"10.1016\/j.cropro.2022.106071","article-title":"Evidence of field-evolved resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) to emamectin benzoate in Brazil","volume":"162","author":"Muraro","year":"2022","journal-title":"Crop Prot."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1093\/jee\/69.4.487","article-title":"Velvetbean caterpillar: A rearing procedure and artificial medium","volume":"69","author":"Greene","year":"1976","journal-title":"J. Econ. Entomol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1002\/ps.4452","article-title":"Insecticide resistance, control failure likelihood and the first law of geography","volume":"73","author":"Guedes","year":"2017","journal-title":"Pest Manag. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. Econ. Entomol."},{"key":"ref_24","unstructured":"R Core Team (2025). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1007\/s10340-020-01220-y","article-title":"Diamide resistance: 10 years of lessons from lepidopteran pests","volume":"93","author":"Richardson","year":"2020","journal-title":"J. Pest Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1002\/ps.4308","article-title":"A retrospective look at anthranilic diamide insecticides: Discovery and lead optimization to chlorantraniliprole and cyantraniliprole","volume":"73","author":"Selby","year":"2017","journal-title":"Pest Manag. Sci."},{"key":"ref_27","unstructured":"Ribeiro, R.S. (2014). Monitoring the Susceptibility to Diamide Insecticides in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) Populations in Brazil. [Master\u2019s Thesis, University of S\u00e3o Paulo]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/ps.5505","article-title":"Detection of a ryanodine receptor target-site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda","volume":"76","author":"Boaventura","year":"2020","journal-title":"Pest Manag. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Okuma, D.M., Cuenca, A., Nauen, R., and Omoto, C. (2022). Large-scale monitoring of the frequency of ryanodine receptor target-site mutations conferring diamide resistance in Brazilian field populations of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects, 13.","DOI":"10.3390\/insects13070626"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s10340-019-01165-x","article-title":"Low risk of resistance evolution of Spodoptera frugiperda to chlorfenapyr in Brazil","volume":"93","author":"Kanno","year":"2020","journal-title":"J. Pest Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2674","DOI":"10.1002\/ps.5812","article-title":"Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: Inheritance and cross-resistance to spinosad","volume":"76","author":"Lira","year":"2020","journal-title":"Pest Manag. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"105921","DOI":"10.1016\/j.pestbp.2024.105921","article-title":"Bulk segregant mapping and transcriptome analyses reveal the molecular mechanisms of spinetoram resistance in Spodoptera frugiperda","volume":"202","author":"Kanno","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Moustafa, M.A.M., El-Said, N.A., Alfuhaid, N.A., Abo-Elinin, F.M.A., Mohamed, R.M.B., and Aioub, A.A.A. (2024). Monitoring and detection of insecticide resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae): Evidence for field-evolved resistance in Egypt. Insects, 15.","DOI":"10.3390\/insects15090705"},{"key":"ref_34","unstructured":"Amado, D. (2017). Characterization of the Susceptibility to Insect Growth Regulator Insecticides in Populations of Helicoverpa armigera and Spodoptera frugiperda (Lepidoptera: Noctuidae) from Brazil. [Master\u2019s Thesis, University of S\u00e3o Paulo]."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jare.2025.06.083","article-title":"Upregulation of a cytochrome P450 gene, CYP6B50, confers multi-insecticide resistance in Spodoptera frugiperda","volume":"82","author":"Guo","year":"2026","journal-title":"J. Adv. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1002\/ps.1753","article-title":"Biochemical Mechanisms of Methoxyfenozide resistance in the cotton leafworm Spodoptera littoralis","volume":"65","author":"Mosallanejad","year":"2009","journal-title":"Pest Manag. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"12887","DOI":"10.1038\/s41598-026-45372-w","article-title":"Biochemical and molecular responses of Spodoptera frugiperda to insecticide exposure: Detoxification enzymes, gene expression, and genotoxic effects","volume":"16","author":"Aburawash","year":"2026","journal-title":"Sci. Rep."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/825\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T04:19:10Z","timestamp":1786508350000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":37,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["insects17080825"],"URL":"https:\/\/doi.org\/10.3390\/insects17080825","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]}}}