{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T08:36:17Z","timestamp":1786523777350,"version":"build-2736575974"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300395"],"award-info":[{"award-number":["32300395"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32300395"],"award-info":[{"award-number":["32300395"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Scientific Research Foundation for Talented Scholars of Anhui Agricultural University","award":["rc342405"],"award-info":[{"award-number":["rc342405"]}]},{"award":["rc342405"],"award-info":[{"award-number":["rc342405"]}],"id":[{"id":"https:\/\/ror.org\/0327f3359","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Spodoptera frugiperda (J.E. Smith) is a notoriously destructive and invasive agricultural pest, causing substantial economic losses worldwide. Research has shown that cytochrome P450s play an important role in detoxifying insecticides. However, the precise molecular mechanism of P450-mediated detoxification of pyrethroids in S. frugiperda remains largely unknown. Enzyme activity assays demonstrated the involvement of P450 monooxygenases in pyrethroid detoxification in S. frugiperda. Subsequently, the results of induction experiments revealed that CYP9A58 and CYP9A59 were significantly upregulated by bifenthrin (up to 4.59- and 5.54-fold) and lambda-cyhalothrin (up to 4.17- and 3.28-fold). Stage- and tissue-specific expression analyses suggested that CYP9A59 exhibited predominant expression in sixth-instar larvae, and CYP9A58 was primarily expressed in the moth stage, with both P450 genes showing high transcript levels in the midgut. The functional role of P450s was confirmed by RNAi, where knockdown of either CYP9A58 or CYP9A59 significantly increased larval susceptibility to lambda-cyhalothrin, raising mortality by 45.71% and 57.68%, respectively. Furthermore, structural modeling demonstrated the capacity of both P450 enzymes\u2014CYP9A58 and CYP9A59\u2014to form stable complexes with pyrethroid insecticides. Collectively, these results confirm that CYP9A58 and CYP9A59 are key mediators of pyrethroid detoxification, conferring insecticide tolerance in S. frugiperda.<\/jats:p>","DOI":"10.3390\/insects17080836","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T07:57:26Z","timestamp":1786521446000},"page":"836","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytochrome P450 Genes CYP9A58 and CYP9A59 Contribute to Pyrethroid Tolerance in Spodoptera frugiperda"],"prefix":"10.3390","volume":"17","author":[{"given":"Dongliang","family":"Su","sequence":"first","affiliation":[{"name":"Key Laboratory of Agri-products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weifang","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agri-products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agri-products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuxia","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agri-products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-1089","authenticated-orcid":false,"given":"Yun","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agri-products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.2307\/3494083","article-title":"A review of the biology of the fall armyworm","volume":"62","author":"Sparks","year":"1979","journal-title":"Fla. Entomol."},{"key":"ref_2","first-page":"e00865","article-title":"Potential distribution of Spodoptera frugiperda (J.E. Smith) in China and the major factors influencing distribution","volume":"21","author":"Wang","year":"2020","journal-title":"Glob. Ecol. Conserv."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Baloch, M.N., Fan, J., Haseeb, M., and Zhang, R. (2020). Mapping potential distribution of Spodoptera frugiperda (Lepidoptera: Noctuidae) in Central Asia. Insects, 11.","DOI":"10.3390\/insects11030172"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/S2095-3119(19)62839-X","article-title":"Case study on the first immigration of fall armyworm, Spodoptera frugiperda invading into China","volume":"20","author":"Sun","year":"2021","journal-title":"J. Integr. Agric."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1111\/1744-7917.12700","article-title":"Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques","volume":"27","author":"Jing","year":"2020","journal-title":"Insect Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4057","DOI":"10.1002\/ps.7602","article-title":"Life table study of Spodoptera frugiperda at different wheat stages and the effect of larval population density on wheat yield","volume":"79","author":"Gong","year":"2023","journal-title":"Pest Manag. Sci."},{"key":"ref_7","first-page":"494","article-title":"Efficacy of insecticides against Spodoptera frugiperda (Smith, 1797)","volume":"11","author":"Fernandes","year":"2019","journal-title":"J. Agric. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2948","DOI":"10.1002\/ps.5407","article-title":"Population genetic structure and demographic history of Spodoptera frugiperda (Lepidoptera: Noctuidae): Implications for insect resistance management programs","volume":"75","author":"Arias","year":"2019","journal-title":"Pest Manag. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5049","DOI":"10.1002\/ps.6545","article-title":"Inheritance patterns, cross-resistance and synergism in Spodoptera frugiperda (Lepidoptera: Noctuidae) resistant to emamectin benzoate","volume":"77","author":"Muraro","year":"2021","journal-title":"Pest Manag. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/S2095-3119(20)63392-5","article-title":"Insecticide resistance monitoring for the invasive populations of fall armyworm, Spodoptera frugiperda in China","volume":"20","author":"Zhang","year":"2021","journal-title":"J. Integr. Agric."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1093\/jee\/toy372","article-title":"Field-evolved resistance of the fall armyworm (Lepidoptera: Noctuidae) to synthetic insecticides in Puerto Rico and Mexico","volume":"112","author":"Blanco","year":"2019","journal-title":"J. Econ. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8007998","DOI":"10.1155\/2022\/8007998","article-title":"Susceptibility evaluation of fall armyworm (Spodoptera frugiperda) infesting maize in Kenya against a range of insecticides","volume":"2022","author":"Gichere","year":"2022","journal-title":"J. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S0965-1748(99)00038-7","article-title":"Cytochrome P450 and insecticide resistance","volume":"29","author":"Scott","year":"1999","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_14","first-page":"326","article-title":"An overview of the main pathways of metabolic resistance in insects","volume":"13","author":"Panini","year":"2016","journal-title":"ISJ-Invert. Surviv. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/s10340-021-01452-6","article-title":"Transgenic overexpression of P450 genes confers deltamethrin resistance in the fall armyworm, Spodoptera frugiperda","volume":"95","author":"Chen","year":"2022","journal-title":"J. Pest Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1007\/s10340-022-01483-7","article-title":"Midgut-specific expression of CYP321A8 P450 gene increases deltamethrin tolerance in the fall armyworm Spodoptera frugiperda","volume":"96","author":"Chen","year":"2023","journal-title":"J. Pest Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1093\/jee\/toad168","article-title":"Function analysis of CYP321A9 from Spodoptera frugiperda (Lepidoptera: Noctuidae) associated with emamectin benzoate, and a novel insecticide, cyproflanilide detoxification","volume":"116","author":"Shi","year":"2023","journal-title":"J. Econ. Entomol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1017\/S0007485314000510","article-title":"Identification of a novel cytochrome P450 gene, CYP321E1 from the diamondback moth, Plutella xylostella (L.) and RNA interference to evaluate its role in chlorantraniliprole resistance","volume":"104","author":"Hu","year":"2014","journal-title":"Bull. Entomol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/1744-7917.12315","article-title":"Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm (Spodoptera litura) and RNA interference to evaluate its role in commonly used insecticides","volume":"24","author":"Wang","year":"2017","journal-title":"Insect Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, R.L., Liu, S.W., Baerson, S.R., Qin, Z., Ma, Z.H., Su, Y.J., and Zhang, J.E. (2018). Identification and functional analysis of a novel cytochrome P450 gene CYP9A105 associated with pyrethroid detoxification in Spodoptera exigua H\u00fcbner. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030737"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"103646","DOI":"10.1016\/j.ibmb.2021.103646","article-title":"Comparative analysis of the detoxification gene inventory of four major Spodoptera pest species in response to xenobiotics","volume":"138","author":"Amezian","year":"2021","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3713","DOI":"10.1002\/ps.6061","article-title":"Toxicological and molecular profiling of insecticide resistance in a Brazilian strain of fall armyworm resistant to Bt Cry1 proteins","volume":"77","author":"Boaventura","year":"2021","journal-title":"Pest Manag. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Cheng, Z., Huang, Z., Yan, B., Huang, X., and Mei, Y. (2024). Comprehensive transcriptomic analysis of Spodoptera frugiperda reveals stage-specific gene expression and P450-mediated adaptation mechanisms. Agronomy, 14.","DOI":"10.20944\/preprints202411.0952.v1"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1038\/s42003-020-01382-6","article-title":"Adaptation by copy number variation increases insecticide resistance in the fall armyworm","volume":"3","author":"Gimenez","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hilliou, F., Chertemps, T., Ma\u00efb\u00e8che, M., and Le Goff, G. (2021). Resistance in the genus Spodoptera: Key insect detoxification genes. Insects, 12.","DOI":"10.3390\/insects12060544"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106599","DOI":"10.1016\/j.pestbp.2025.106599","article-title":"Monitoring the molecular mechanisms of insecticide resistance in Spodoptera frugiperda populations from Thailand","volume":"214","author":"Lueke","year":"2025","journal-title":"Pestic. Biochem Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.pestbp.2007.03.001","article-title":"Beta-cypermethrin resistance associated with high carboxylesterase activities in a strain of house fly, Musca domestica (Diptera: Muscidae)","volume":"89","author":"Zhang","year":"2007","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/0048-3575(79)90057-9","article-title":"Glutathione Stransferase and hydrolytic activity in a tetrachlorvinphos-resistant strain of housefly and their influence on resistance","volume":"111","author":"Oppenoorth","year":"1979","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s10340-017-0933-1","article-title":"Uptake of quercetin reduces larval sensitivity to lambda-cyhalothrin in Helicoverpa armigera","volume":"91","author":"Chen","year":"2018","journal-title":"J. Pest Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Han, S., Qin, Q., Wang, D., Zhou, Y., and He, Y. (2021). Selection and Evaluation of reference genes for qRT-PCR in Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects, 12.","DOI":"10.3390\/insects12100902"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"121698","DOI":"10.1016\/j.jhazmat.2019.121698","article-title":"Activation of CncC pathway by ROS burst regulates cytochrome P450 CYP6AB12 responsible for lambda-cyhalothrin tolerance in Spodoptera litura","volume":"387","author":"Lu","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1111\/imb.12829","article-title":"Knockdown of CYP9A9 increases the susceptibility to lufenuron, methoxyfenozide and a mixture of both in Spodoptera exigua","volume":"32","author":"Zhang","year":"2023","journal-title":"Insect Mol. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"15206","DOI":"10.1073\/pnas.1202047109","article-title":"Resistance of Australian Helicoverpa armigera to fenvalerate is due to the chimeric P450 enzyme CYP337B3","volume":"109","author":"Agnolet","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.pestbp.2015.01.006","article-title":"Cytochrome P450s-Their expression, regulation, and role in insecticide resistance","volume":"120","author":"Liu","year":"2015","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1146\/annurev-ento-070621-061328","article-title":"The Role of Cytochrome P450s in insect toxicology and resistance","volume":"67","author":"Nauen","year":"2022","journal-title":"Annu. Rev. Entomol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1093\/jee\/toaf003","article-title":"Responses of biological characteristics and detoxification enzymes in the fall armyworm to methoxyfenozide stress","volume":"118","author":"Yang","year":"2025","journal-title":"J. Econ. Entomol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1002\/ps.7355","article-title":"Cytochrome P450s genes CYP321A9 and CYP9A58 contribute to host plant adaptation in the fall armyworm Spodoptera frugiperda","volume":"79","author":"He","year":"2023","journal-title":"Pest Manag. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"105916","DOI":"10.1016\/j.pestbp.2024.105916","article-title":"Investigation of lambda-cyhalothrin resistance in Spodoptera frugiperda: Heritability, cross-resistance, and mechanisms","volume":"202","author":"Guo","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"106009","DOI":"10.1016\/j.pestbp.2024.106009","article-title":"Molecular insights into the functional analysis of P450 CYP321A7 gene in the involvement of detoxification of lambda-cyhalothrin in Spodoptera frugiperda","volume":"203","author":"Li","year":"2024","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"21812","DOI":"10.1038\/s41598-025-99196-1","article-title":"Genetic variation associated with increased lambda-cyhalothrin resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) in West Africa","volume":"15","author":"Tossou","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4371","DOI":"10.1111\/j.1365-294X.2012.05548.x","article-title":"Gossypol-enhanced P450 gene pool contributes to cotton bollworm tolerance to a pyrethroid insecticide","volume":"21","author":"Tao","year":"2012","journal-title":"Mol. Ecol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.pestbp.2016.07.001","article-title":"RNA interference of three up-regulated transcripts associated with insecticide resistance in an imidacloprid resistant population of Leptinotarsa decemlineata","volume":"135","author":"Clements","year":"2017","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.pestbp.2009.08.014","article-title":"Cross-strain comparison of cypermethrin-induced cytochrome P450 transcription under different induction conditions in diamondback moth","volume":"96","author":"Baek","year":"2010","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/imb.12140","article-title":"Cytochrome P450s from the fall armyworm (Spodoptera frugiperda): Responses to plant allelochemicals and pesticides","volume":"24","author":"Giraudo","year":"2015","journal-title":"Insect. Mol. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1093\/jee\/tox155","article-title":"Expression differences of resistance-related genes induced by cycloxaprid using qRT-PCR in the female adult of Sogatella furcifera (Hemiptera: Delphacidae)","volume":"110","author":"Jin","year":"2017","journal-title":"J. Econ. Entomol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Shu, H.J., Lin, Y.F., Zhang, Z.B., Qiu, L., Ding, W.B., Gao, Q., Xue, J., Li, Y.Z., and He, H.L. (2023). The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin. BMC Genom., 24.","DOI":"10.1186\/s12864-022-09095-2"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1042\/BST0341252","article-title":"Evolution of insect P450","volume":"34","author":"Feyereisen","year":"2006","journal-title":"Biochem. Soc. Trans."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10886-014-0540-z","article-title":"Expression analysis of two P450 monooxygenase genes of the tobacco cutworm moth (Spodoptera litura) at different developmental stages and in response to plant allelochemicals","volume":"41","author":"Wang","year":"2015","journal-title":"J. Chem. Ecol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jinsphys.2015.03.013","article-title":"Two homologous carboxylesterase genes from Locusta migratoria with different tissue expression patterns and roles in insecticide detoxification","volume":"77","author":"Zhang","year":"2015","journal-title":"J. Insect. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/1744-7917.12538","article-title":"The expression of Spodoptera exigua P450 and UGT genes: Tissue specificity and response to insecticides","volume":"26","author":"Hu","year":"2019","journal-title":"Insect Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8557","DOI":"10.1073\/pnas.1000059107","article-title":"A brain-specific cytochrome P450 responsible for the majority of deltamethrin resistance in the QTC279 strain of Tribolium castaneum","volume":"107","author":"Zhu","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Hafeez, M., Li, X., Ullah, F., Zhang, Z., Zhang, J., Huang, J., Fern\u00e1ndez-Grandon, G.M., Khan, M.M., Siddiqui, J.A., and Chen, L. (2022). Downregulation of P450 genes enhance susceptibility to indoxacarb and alters physiology and development of fall armyworm, Spodoptera frugipreda (Lepidoptera: Noctuidae). Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.884447"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"14989\u221215002","DOI":"10.1021\/acs.jafc.3c05423","article-title":"CYP321A Subfamily P450s contribute to the detoxification of phytochemicals and pyrethroids in Spodoptera litura","volume":"71","author":"Xiao","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.taap.2017.11.010","article-title":"Age dependent in vitro metabolism of bifenthrin in rat and human hepatic microsomes","volume":"338","author":"Nallani","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s13071-021-04609-5","article-title":"Assessing cross-resistance within the pyrethroids in terms of their interactions with key cytochrome P450 enzymes and resistance in vector populations","volume":"14","author":"Moyes","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"104871","DOI":"10.1016\/j.pestbp.2021.104871","article-title":"Pyrethrin-resembling pyrethroids are metabolized more readily than heavily modified ones by CYP9As from Helicoverpa armigera","volume":"176","author":"Tian","year":"2021","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Liu, Z., Wu, F., Liang, W., Zhou, L., and Huang, J. (2022). Molecular mechanisms underlying metabolic resistance to cyflumetofen and bifenthrin in Tetranychus urticae koch on cowpea. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232416220"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1007\/s10886-013-0335-7","article-title":"Structure and function of cytochrome P450s in insect adaptation to natural and synthetic toxins: Insights gained from molecular modeling","volume":"39","author":"Schuler","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/1744-7917.13376","article-title":"P450 gene CYP321A8 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda","volume":"32","author":"Chen","year":"2025","journal-title":"Insect Sci."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Al Naggar, Y., Fahmy, N.M., Alkhaibari, A.M., Al-Akeel, R.K., Alharbi, H.M., Mohamed, A., Eleftherianos, I., El-Seedi, H.R., Giesy, J.P., and Alharbi, H.A. (2025). Mechanisms and genetic drivers of resistance of insect pests to insecticides and approaches to its control. Toxics, 13.","DOI":"10.3390\/toxics13080681"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.cropro.2025.107322","article-title":"An overview of insecticide resistance mechanisms, challenges, and management strategies in Spodoptera frugiperda","volume":"197","author":"Ngegba","year":"2025","journal-title":"Crop Prot."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/836\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T08:13:10Z","timestamp":1786522390000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/8\/836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":63,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["insects17080836"],"URL":"https:\/\/doi.org\/10.3390\/insects17080836","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]}}}