{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:19:04Z","timestamp":1787635144223,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"crossref","award":["2026ZD01908804"],"award-info":[{"award-number":["2026ZD01908804"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Identifying conserved RNAi targets that remain effective across mosquito species and resistance backgrounds is important for developing broadly applicable mosquito control tools. Here, an engineered Escherichia coli HT115-L4440 system was used to produce \u03b2-tubulin double-stranded RNA (dsRNA), and its larvicidal activity was evaluated in susceptible and pyrethroid-resistant strains of Culex quinquefasciatus and Aedes albopictus. Recombinant bacteria produced species-specific \u03b2-tubulin dsRNA fragments of approximately 360 bp. Immersion treatment caused high larval mortality in both species. In Cx. quinquefasciatus, mortality reached 85.00% and 89.00% in susceptible and resistant strains, respectively, compared with 8.00% and 10.00% in controls, while \u03b2-tubulin transcript abundance showed maximum reductions of 571-fold and 333-fold, respectively. In Ae. albopictus, mortality reached 91.00% and 82.00% in susceptible and resistant strains, compared with 9% and 8% in controls, with maximum reductions in \u03b2-tubulin expression of 157-fold and 337-fold, respectively. These comparable effects suggest that \u03b2-tubulin dsRNA is not compromised by conventional pyrethroid-resistance mechanisms. Transcriptomic and proteomic analyses showed that \u03b2-tubulin knockdown disrupted key cellular processes, including cytoskeletal regulation, metabolism, protein synthesis, intracellular transport, detoxification, oxidative stress, DNA replication, and autophagy. Overall, engineered bacteria-derived \u03b2-tubulin dsRNA induced consistent gene silencing and larval mortality in both susceptible and resistant mosquito larvae, supporting \u03b2-tubulin as a conserved candidate RNAi target for mosquito control.<\/jats:p>","DOI":"10.3390\/insects17090880","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T14:49:34Z","timestamp":1787582974000},"page":"880","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engineered Escherichia coli-Derived dsRNA Identifies \u03b2-Tubulin as a Candidate RNAi Target in Mosquito Larvae"],"prefix":"10.3390","volume":"17","author":[{"given":"Kai","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 561113, China"},{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongdan","family":"Bi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2143-3350","authenticated-orcid":false,"given":"Teng","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxi","family":"Pan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 561113, China"},{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"},{"name":"Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahong","family":"Wu","sequence":"additional","affiliation":[{"name":"Characteristic Key Laboratory of Modern Pathogen Biology, School of Basic Medicine Sciences, Guizhou Medical University, Gui-an New Area 561113, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Moyes, C.L., Vontas, J., Martins, A.J., Ng, L.C., Koou, S.Y., Dusfour, I., Raghavendra, K., Pinto, J., Corbel, V., and David, J.P. 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