{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:17:14Z","timestamp":1787807834630,"version":"build-2784847793"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key R&amp;D Program of China","award":["2023YFD1400600"],"award-info":[{"award-number":["2023YFD1400600"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302395"],"award-info":[{"award-number":["32302395"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32302395"],"award-info":[{"award-number":["32302395"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["CQMAITS202305"],"award-info":[{"award-number":["CQMAITS202305"]}]},{"name":"Yunnan Scientific Research Project","award":["202405AF140085"],"award-info":[{"award-number":["202405AF140085"]}]},{"DOI":"10.13039\/501100006250","name":"Southwest University","doi-asserted-by":"publisher","award":["SWU-XJLJ202310"],"award-info":[{"award-number":["SWU-XJLJ202310"]}],"id":[{"id":"10.13039\/501100006250","id-type":"DOI","asserted-by":"publisher"}]},{"award":["SWU-XJLJ202310"],"award-info":[{"award-number":["SWU-XJLJ202310"]}],"id":[{"id":"https:\/\/ror.org\/01kj4z117","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Neoseiulus barkeri is an important predatory mite used for biological control in citrus orchards in China. Systematic studies on the toxicity of commonly used pesticides to predatory mites are rarely reported. In this study, we developed a \u201cfour-well volcanic dish\u201d bioassay system to determine the acute contact toxicity of 33 pesticides registered in citrus, and further evaluated the sublethal effects of chlorpyrifos, lambda-cyhalothrin, and chlorfenapyr at LC10 and LC20 on the development and reproduction of N. barkeri. Amitraz was the most toxic compound, with an LC50 of 55.92 mg\/L. Chlorpyrifos, chlorfenapyr, lambda-cyhalothrin, glufosinate-ammonium, and beta-cyfluthrin also showed relatively high toxicity, with LC50 values of 100\u2013400 mg\/L, whereas many other pesticides were much less toxic, with LC50 values above 1000 mg\/L. LC20 exposure to chlorpyrifos, lambda-cyhalothrin, or chlorfenapyr significantly prolonged development, reduced fecundity, and shortened female longevity. LC20 treatments also decreased the net reproductive rate (R0) and intrinsic rate of increase (rm), indicating reduced population growth potential. This study clarified the toxic effects of various pesticides on predatory mites and further elucidated the sublethal effects of three broad-spectrum pesticides on these mites. These findings provide a theoretical basis for coordinating chemical and biological control in citrus IPM programs.<\/jats:p>","DOI":"10.3390\/insects17090891","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:01:07Z","timestamp":1787670067000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toxic and Sublethal Effects of Commonly Used Pesticides on Neoseiulus barkeri (Acari: Phytoseiidae)"],"prefix":"10.3390","volume":"17","author":[{"given":"Yu-Fei","family":"Chen","sequence":"first","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wu","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chao","family":"Shen","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng-Ye","family":"Yin","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0421-990X","authenticated-orcid":false,"given":"Si-Chen","family":"Li","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Liu","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Pan","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1680-4228","authenticated-orcid":false,"given":"Lin","family":"Cong","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan-Fu","family":"Yao","sequence":"additional","affiliation":[{"name":"Yuxi Academy of Agricultural Sciences, Yuxi 653100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Jiang","family":"Yu","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Ran","sequence":"additional","affiliation":[{"name":"Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Chongqing 400712, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"ref_1","unstructured":"Demite, P.R., de Moraes, G.J., McMurtry, J.A., Denmark, H.A., and Castilho, R.C. 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