{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:20:38Z","timestamp":1788326438617,"version":"build-2803163510"},"reference-count":68,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32202294"],"award-info":[{"award-number":["32202294"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32202294"],"award-info":[{"award-number":["32202294"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Jiangsu Liuqiao Grain Reserve Direct Depot Co., Ltd.","award":["2185072511"],"award-info":[{"award-number":["2185072511"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>It has been suggested that hypoxia-inducible factor 1\u03b1 (HIF1\u03b1) serves as a key regulatory factor in insect metabolism remodelling under hypoxia, in which lipid metabolism appears to play an important role. However, the underlying mechanism remains incompletely understood. In this paper, the function of HIF1\u03b1 in regulating lipid metabolism under hypoxia in Callosobruchus chinensis (Coleoptera: Bruchidae), a typical stored-product insect, was investigated. Based on the amplification and cloning, the sequence of the CcHIF1\u03b1 gene was analysed and found to be highly conserved throughout evolution. Then HIF1\u03b1 expression in the adult C. chinensis was knocked down using RNA interference (RNAi) via feeding methods. After HIF1\u03b1 knockdown, insects under hypoxic conditions exhibited higher mortality, along with decreased activities of four typical antioxidant enzymes (CAT, POD, GST, and SOD) and increased levels of two peroxide products (ROS and LPO), compared to those of the control. Moreover, comprehensive lipidomic analysis using UHPLC-QE\u2013MS\/MS revealed that lipid metabolism was reprogrammed, with significant changes in the levels of lipids such as SM, LPC, PI, SQDG, MAG, PE, ACar, and TAG. In particular, the increase in ceramide (Cer) or Cer-related lipids was observed to be associated with the production of peroxides. ELISA assays further verified the effect of HIF1\u03b1 on changes in these characteristic lipids, such as the increase in PA and Cer and a decrease in TAG, PC and SM. Correspondingly, the expressions of the key genes (i.e., SPTLC1, KDSR, SMPD3, SGPL1, DGAT) involved in sphingolipid metabolism changed following HIF1\u03b1 knockdown. These results are consistent with the possibility that HIF1\u03b1-mediated regulation of lipid metabolism may be important for insects facing hypoxic environments, and these findings could provide an in-depth understanding of insect hypoxia adaptation, thereby potentially contributing to the development of innovative pest management strategies.<\/jats:p>","DOI":"10.3390\/insects17090904","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:41:18Z","timestamp":1788266478000},"page":"904","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Function of HIF-1\u03b1 in Regulating Sphingolipid Metabolism and Alleviating Oxidative Stress Damage in Callosobruchus chinensis Under Hypoxia"],"prefix":"10.3390","volume":"17","author":[{"given":"Xiao","family":"Li","sequence":"first","affiliation":[{"name":"School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"},{"name":"School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2753-514X","authenticated-orcid":false,"given":"Sufen","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Zhichao","family":"Wan","sequence":"additional","affiliation":[{"name":"School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"},{"name":"School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xuemei","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Yutong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xueqing","family":"Geng","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7171-7899","authenticated-orcid":false,"given":"Yujie","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jspr.2018.05.010","article-title":"Hermetic storage as an alternative for controlling Callosobruchus maculatus (Coleoptera: Chrysomelidae) and preserving the quality of cowpeas","volume":"78","author":"Silva","year":"2018","journal-title":"J. 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