{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T05:16:43Z","timestamp":1788412603160,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501898"],"award-info":[{"award-number":["31501898"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["31501898"],"award-info":[{"award-number":["31501898"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Key Disciplines Projects for Universities","award":["2025ZDZX2045"],"award-info":[{"award-number":["2025ZDZX2045"]}]},{"name":"Guangzhou University\u2019s Exploration Experiment Construction Project","award":["SJ202412"],"award-info":[{"award-number":["SJ202412"]}]},{"award":["SJ202412"],"award-info":[{"award-number":["SJ202412"]}],"id":[{"id":"https:\/\/ror.org\/05ar8rn06","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangzhou University Provincial-Level College Student Innovation Training Program","award":["S202511078168"],"award-info":[{"award-number":["S202511078168"]}]},{"award":["S202511078168"],"award-info":[{"award-number":["S202511078168"]}],"id":[{"id":"https:\/\/ror.org\/05ar8rn06","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangzhou University College Student Innovation Training Program","award":["XJ202511078336; XJ202611078416; XJ202611078426"],"award-info":[{"award-number":["XJ202511078336; XJ202611078416; XJ202611078426"]}]},{"award":["XJ202511078336; XJ202611078416; XJ202611078426"],"award-info":[{"award-number":["XJ202511078336; XJ202611078416; XJ202611078426"]}],"id":[{"id":"https:\/\/ror.org\/05ar8rn06","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Insects lack adaptive immunity and rely exclusively on innate immune system for pathogen defense. However, the specific role of BmToll9-2, a key Toll receptor in the silkworm (Bombyx mori), in mediating immune responses, particularly against bacterial challenges in the larval fat body, remains incompletely understood. To address this gap, this study employed a combination of molecular approaches, including RNA interference (RNAi) targeting BmToll9-2, bacterial challenges with heat-inactivated Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), and quantitative real-time PCR (qPCR) to assess the transcriptional changes of BmToll9-2 and immune-related genes in the fat body, a key immune tissue. Quantitative analysis showed that after BmToll9-2 RNAi, bacterial challenges significantly upregulated BmToll9-2 expression in the fat body at 12 h post-challenge, whereas BmToll9-2 silencing alone notably downregulated most downstream signaling genes of the Toll pathway by 53.07\u201383.14%, as well as 11 immune effector genes, including antimicrobial peptide genes, by 64.48\u201393.40%. Importantly, feeding bacteria post-RNAi reversed these downregulations: E. coli induced a stronger upregulation of both signaling and effector genes compared to S. aureus. This study provides transcriptional evidence that BmToll9-2 may act as a positive regulator of the Toll pathway signaling and antimicrobial peptides in silkworm larval fat body, facilitating robust and rapid immune signaling. This study deepens our understanding of Lepidopteran innate immunity by elucidating key molecular mechanisms, thereby providing a solid foundation for refining RNAi-based pest control strategies.<\/jats:p>","DOI":"10.3390\/insects17090905","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:41:18Z","timestamp":1788266478000},"page":"905","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Crosstalk Between BmToll9-2, the Toll Pathway, and Antimicrobial Peptides in the Silkworm (Bombyx mori) Larval Fat Body"],"prefix":"10.3390","volume":"17","author":[{"given":"Ruixuan","family":"Lin","sequence":"first","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyuan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Lv","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuying","family":"He","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintong","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiling","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingrong","family":"Li","sequence":"additional","affiliation":[{"name":"The Sericultural and Agri-Food Research Institute of the Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0857-4298","authenticated-orcid":false,"given":"Jisheng","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jinsphys.2012.07.004","article-title":"Beyond RNAi: Antiviral defense strategies in Drosophila and mosquito","volume":"59","author":"Merkling","year":"2013","journal-title":"J. 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