{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:21:59Z","timestamp":1788268919760,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yuelushan Laboratory Breeding Program","award":["YSL-2025-ZY02036"],"award-info":[{"award-number":["YSL-2025-ZY02036"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is the primary vector of Candidatus Liberibacter asiaticus, the causal agent of huanglongbing, the most devastating disease of citrus worldwide. Understanding its population structure and developing lineage-specific markers are important for surveillance, quarantine and pest management. Here, 133 complete mitochondrial genomes were analyzed, comprising 77 previously published sequences, 13 newly assembled genomes and 43 metagenome-assembled mitogenomes. Phylogenetic analysis revealed two highly differentiated mitochondrial lineages\u2014an Asian lineage (n = 91, including the Uruguay sample) and an American lineage (n = 42, including the Pakistan sample) (FST = 0.560, p &lt; 0.001)\u2014separated by 32 fixed variants (132 genomes, excluding the reference sequence MF426268.1). Within the Asian lineage, the Yunnan\u2013Guizhou Plateau population showed significant differentiation from lowland populations (FST = 0.130, p &lt; 0.05), reduced nucleotide diversity and moderately supported monophyletic clustering consistent with long-term isolation, while demographic analyses suggested that Southeast China may have acted as a post-colonization expansion center. Candidate lineage-specific markers, including variants in trnG and rrn16, provide molecular resources for population-origin assignment, quarantine surveillance and pest monitoring; these candidates require validation across broader geographic sampling.<\/jats:p>","DOI":"10.3390\/insects17090917","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:41:18Z","timestamp":1788266478000},"page":"917","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mitochondrial Genomes Reveal Global Population Structure, Lineage Divergence, and Candidate Diagnostic Markers of Diaphorina citri"],"prefix":"10.3390","volume":"17","author":[{"given":"Lijuan","family":"Yin","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Suming","family":"Dai","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"College of Horticulture, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Dan","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Horticulture, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Dazhi","family":"Li","sequence":"additional","affiliation":[{"name":"Yuelushan Laboratory, Changsha 410128, China"},{"name":"College of Horticulture, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Yunsheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1146\/annurev-ento-120811-153542","article-title":"Biology and management of Asian citrus psyllid, vector of the huanglongbing pathogens","volume":"58","author":"Stelinski","year":"2013","journal-title":"Annu. Rev. Entomol."},{"key":"ref_2","first-page":"7","article-title":"Huanglongbing: A destructive, newly-emerging, century-old disease of citrus","volume":"88","year":"2006","journal-title":"J. Plant Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1094\/PHYTO-12-12-0331-RVW","article-title":"Citrus huanglongbing: A newly relevant disease presents unprecedented challenges","volume":"103","author":"Wang","year":"2013","journal-title":"Phytopathology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1146\/annurev-phyto-073009-114418","article-title":"Current epidemiological understanding of citrus huanglongbing","volume":"48","author":"Gottwald","year":"2010","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/jipb.12437","article-title":"Huanglongbing: An overview of a complex pathosystem ravaging the world\u2019s citrus","volume":"58","author":"Douhan","year":"2016","journal-title":"J. Integr. Plant Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Singerman, A., and Useche, P. (2016). Impact of Citrus Greening on Citrus Operations in Florida, University of Florida IFAS Extension.","DOI":"10.32473\/edis-fe983-2016"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1653\/0015-4040(2004)087[0330:ACPSPA]2.0.CO;2","article-title":"Asian citrus psyllids (Sternorrhyncha: Psyllidae) and greening disease of citrus: A literature review and assessment of risk in Florida","volume":"87","author":"Halbert","year":"1998","journal-title":"Fla. Entomol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s10658-015-0804-7","article-title":"Global climate suitability of citrus huanglongbing and its vector, the Asian citrus psyllid, using two correlative species distribution modeling approaches, with emphasis on the USA","volume":"144","author":"Halbert","year":"2016","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1653\/0015-4040(2004)087[0401:DOTACP]2.0.CO;2","article-title":"Distribution of the Asian citrus psyllid, Diaphorina citri Kuwayama (Rhynchota: Psyllidae) in the Caribbean basin","volume":"87","author":"Halbert","year":"2004","journal-title":"Fla. Entomol."},{"key":"ref_10","unstructured":"Yamamoto, P.T., Felippe, M.R., Garbim, L.F., Coelho, J.H.C., Ximenes, N.L., Martins, E.C., Leite, A.P.R., Sousa, M.C., Abrah\u00e3o, D.P., and Braz, J.D. (2006, January 16\u201320). Diaphorina citri (Kuwayama) (Hemiptera: Psyllidae): Vector of the bacterium \u201cCandidatus Liberibacter americanus\u201d. Proceedings of the Huanglongbing-Greening International Workshop, S\u00e3o Paulo, Brazil."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/s10658-016-0921-y","article-title":"First occurrence of Diaphorina citri in East Africa, characterization of the Ca. Liberibacter species causing huanglongbing (HLB) in Tanzania, and potential further spread of D. citri and HLB in Africa and Europe","volume":"146","author":"Shimwela","year":"2016","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1051\/fruits:2001131","article-title":"Biological control of Diaphorina citri (Hemiptera: Psyllidae) in Guadeloupe by imported Tamarixia radiata (Hymenoptera: Eulophidae)","volume":"56","author":"Quilici","year":"2001","journal-title":"Fruits"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/eea.12025","article-title":"Asian citrus psyllid, Diaphorina citri, vector of huanglongbing disease","volume":"146","author":"Hall","year":"2013","journal-title":"Entomol. Exp. Appl."},{"key":"ref_14","first-page":"1787","article-title":"Overview of worldwide diversity of Diaphorina citri Kuwayama mitochondrial cytochrome oxidase 1 haplotypes: A tool for huanglongbing management","volume":"105","author":"Boykin","year":"2012","journal-title":"J. Econ. Entomol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Fuentes, A., Braswell, W.E., Ruiz-Arce, R., and Racelis, A. (2018). Genetic variation and population structure of Diaphorina citri using cytochrome oxidase I sequencing. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0198399"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/ece3.3680","article-title":"Global mtDNA genetic structure and hypothesized invasion history of a major pest of citrus, Diaphorina citri (Hemiptera: Liviidae)","volume":"8","author":"Luo","year":"2018","journal-title":"Ecol. Evol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1093\/jee\/toz046","article-title":"Diaphorina citri (Hemiptera: Psylloidea) in China: Two invasion routes and three transmission paths","volume":"112","author":"Zhang","year":"2019","journal-title":"J. Econ. Entomol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4541","DOI":"10.1111\/j.1365-294X.2009.04380.x","article-title":"Mitochondrial DNA as a marker of molecular diversity: A reappraisal","volume":"18","author":"Galtier","year":"2009","journal-title":"Mol. Ecol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1093\/aesa\/87.6.651","article-title":"Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers","volume":"87","author":"Simon","year":"1994","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1146\/annurev-ento-011613-162007","article-title":"Insect mitochondrial genomics: Implications for evolution and phylogeny","volume":"59","author":"Cameron","year":"2014","journal-title":"Annu. Rev. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.tree.2014.10.008","article-title":"DNA barcodes for ecology, evolution, and conservation","volume":"30","author":"Kress","year":"2015","journal-title":"Trends Ecol. Evol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1002\/ps.5044","article-title":"Population diversity of Diaphorina citri (Hemiptera: Liviidae) in China based on whole mitochondrial genome sequences","volume":"74","author":"Wu","year":"2018","journal-title":"Pest Manag. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1744-7348.2000.tb00060.x","article-title":"Effects of temperature on biology and life table parameters of the Asian citrus psyllid, Diaphorina citri Kuwayama (Homoptera: Psyllidae)","volume":"137","author":"Liu","year":"2000","journal-title":"Ann. Appl. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.biocontrol.2009.04.001","article-title":"Exclusion techniques reveal significant biotic mortality suffered by Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae) populations in Florida citrus","volume":"50","author":"Qureshi","year":"2009","journal-title":"Biol. Control"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/brv.12107","article-title":"The role of the uplift of the Qinghai-Tibetan Plateau for the evolution of Tibetan biotas","volume":"90","author":"Favre","year":"2015","journal-title":"Biol. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"dsac027","DOI":"10.1093\/dnares\/dsac027","article-title":"High-quality, chromosome-scale genome assemblies: Comparisons of three Diaphorina citri (Asian citrus psyllid) geographic populations","volume":"29","author":"Carlson","year":"2022","journal-title":"DNA Res."},{"key":"ref_27","unstructured":"Wei, D., Yin, L., Xia, Y., Qiu, T., Li, N., Zhang, Y., Yang, Z., Li, D., and Dai, S. (South China Fruits, 2026). Genetic diversity and population structure analysis of Diaphorina citri in Hunan Province, South China Fruits, (In Chinese)."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1094\/PHYTO-02-22-0067-R","article-title":"Strain tracking of \u2018Candidatus Liberibacter asiaticus\u2019, the citrus greening pathogen, by high-resolution microbiome analysis of Asian citrus psyllids","volume":"112","author":"Higgins","year":"2022","journal-title":"Phytopathology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: Improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"msag117","DOI":"10.1093\/molbev\/msag117","article-title":"IQ-TREE 3: Phylogenomic inference software using complex evolutionary models","volume":"43","author":"Wong","year":"2026","journal-title":"Mol. Biol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: Fast model selection for accurate phylogenetic estimates","volume":"14","author":"Kalyaanamoorthy","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1093\/molbev\/msx248","article-title":"DnaSP 6: DNA sequence polymorphism analysis of large data sets","volume":"34","author":"Rozas","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1111\/2041-210X.12410","article-title":"POPART: Full-feature software for haplotype network construction","volume":"6","author":"Leigh","year":"2015","journal-title":"Methods Ecol. Evol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1093\/bioinformatics\/btt763","article-title":"DIYABC v2.0: A software to make approximate Bayesian computation inferences about population history using single nucleotide polymorphism, DNA sequence and microsatellite data","volume":"30","author":"Cornuet","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1093\/bioinformatics\/btv684","article-title":"Reliable ABC model choice via random forests","volume":"32","author":"Pudlo","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Pickrell, J.K., and Pritchard, J.K. (2012). Inference of population splits and mixtures from genome-wide allele frequency data. PLoS Genet., 8.","DOI":"10.1038\/npre.2012.6956.1"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1755-0998.2010.02847.x","article-title":"Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows","volume":"10","author":"Excoffier","year":"2010","journal-title":"Mol. Ecol. Resour."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1098\/rstb.2005.1713","article-title":"DNA barcodes for biosecurity: Invasive species identification","volume":"360","author":"Armstrong","year":"2005","journal-title":"Philos. Trans. R. Soc. B"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1093\/jhered\/89.5.415","article-title":"Shallow population histories in deep evolutionary lineages of marine fishes: Insights from sardines and anchovies and lessons for conservation","volume":"89","author":"Grant","year":"1998","journal-title":"J. Hered."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5948","DOI":"10.1073\/pnas.100110397","article-title":"Reduced genetic variation and the success of an invasive species","volume":"97","author":"Tsutsui","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/9\/917\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:55:35Z","timestamp":1788267335000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/17\/9\/917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":40,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["insects17090917"],"URL":"https:\/\/doi.org\/10.3390\/insects17090917","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}