{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:58:00Z","timestamp":1790751480912,"version":"4.1.0"},"reference-count":131,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2016,10,30]],"date-time":"2016-10-30T00:00:00Z","timestamp":1477785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institues of Health, National Institute of Allergy and Infectious Diseases","award":["R56-AI110721"],"award-info":[{"award-number":["R56-AI110721"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Dengue (DENV), yellow fever, chikungunya, and Zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. Besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, Aedes aegypti. The \u201cvectorial capacity\u201d of A. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmit a given arbovirus. This intrinsic ability is known as \u201cvector competence\u201d. Based on whole transcriptome analysis, several genes and pathways have been predicated to have an association with a susceptible or refractory response in A. aegypti to DENV infection. However, the functional genomics of vector competence of A. aegypti is not well understood, primarily due to lack of integrative approaches in genomic or transcriptomic studies. In this review, we focus on the present status of genomics studies of DENV vector competence in A. aegypti as limited information is available relative to the other arboviruses. We propose future areas of research needed to facilitate the integration of vector and virus genomics and environmental factors to work towards better understanding of vector competence and vectorial capacity in natural conditions.<\/jats:p>","DOI":"10.3390\/insects7040058","type":"journal-article","created":{"date-parts":[[2016,10,31]],"date-time":"2016-10-31T11:09:46Z","timestamp":1477912186000},"page":"58","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Genome Investigations of Vector Competence in Aedes aegypti to Inform Novel Arbovirus Disease Control Approaches"],"prefix":"10.3390","volume":"7","author":[{"given":"David","family":"Severson","sequence":"first","affiliation":[{"name":"Department of Biological Sciences and Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanta","family":"Behura","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Brady, O.B., Gething, P.W., Bhatt, S., Messina, J.P., Brownstein, J.S., Hoen, A.G., Moyes, C.L., Farlow, A.W., Scott, T.W., and Hay, S.I. (2012). Refining the global spatial limits of dengue virus transmission by evidence-based consensus. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001760"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jcv.2014.08.030","article-title":"Yellow fever","volume":"64","author":"Monath","year":"2015","journal-title":"J. Clin. Virol."},{"key":"ref_4","first-page":"211","article-title":"Chikungunya virus infections: An overview","volume":"36","author":"Caglioti","year":"2013","journal-title":"New Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.antiviral.2016.03.010","article-title":"Zika virus: History, emergence, biology, and prospects for control","volume":"130","author":"Weaver","year":"2016","journal-title":"Antivir. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e77","DOI":"10.1038\/emi.2016.74","article-title":"Vaccines and immunization strategies for dengue prevention","volume":"5","author":"Liu","year":"2016","journal-title":"Emerg. Microbes Infect."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"D24","DOI":"10.1016\/j.vaccine.2015.05.095","article-title":"Trials and tribulations on the path to developing a dengue vaccine","volume":"33","author":"Thomas","year":"2015","journal-title":"Vaccine"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1146\/annurev.ento.45.1.371","article-title":"Insecticide resistance in insect vectors of human disease","volume":"45","author":"Hemingway","year":"2000","journal-title":"Annu. Rev. Entomol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1146\/annurev-ento-010814-020828","article-title":"Insecticide resistance in mosquitoes: Impact, mechanisms, and research directions","volume":"60","author":"Liu","year":"2015","journal-title":"Annu. Rev. Entomol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1179\/2047773213Y.0000000095","article-title":"Genetic control of Aedes mosquitoes","volume":"107","author":"Alphey","year":"2013","journal-title":"Pathog. Glob. Health"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev-ento-011613-162002","article-title":"Genetic control of mosquitoes","volume":"59","author":"Alphey","year":"2014","journal-title":"Annu. Rev. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s40475-013-0007-2","article-title":"Novel genetic and molecular tools for the investigation and control of dengue virus transmission by mosquitoes","volume":"1","author":"Franz","year":"2014","journal-title":"Curr. Trop. Med. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1038\/nbt.2350","article-title":"Successful suppression of a field mosquito population by sustained release of engineering male mosquitoes","volume":"30","author":"Harris","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4772","DOI":"10.1073\/pnas.1019295108","article-title":"Genetic elimination of dengue vector mosquitoes","volume":"108","author":"Nimmo","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/nature10355","article-title":"The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations","volume":"476","author":"Walker","year":"2011","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"249","DOI":"10.3390\/ijerph10010249","article-title":"Nature, nurture and evolution of intra-species variation in mosquito arbovirus transmission competence","volume":"10","author":"Tabachnick","year":"2013","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3741","DOI":"10.3390\/v7072795","article-title":"Tissue barriers to arbovirus infection in mosquitoes","volume":"7","author":"Franz","year":"2015","journal-title":"Viruses"},{"key":"ref_18","unstructured":"Marquardt, W.C. (2005). Biology of Disease Vectors, Elsevier. [2nd ed.]."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Lambrechts, L., Chevillon, C., Albright, R.G., Thaisomboonsuk, B., Richardson, J.H., Jarman, R.G., and Scott, T.W. (2009). Genetic specificity and potential for local adaptation between dengue viruses and mosquito vectors. BMC Evol. Biol.","DOI":"10.1186\/1471-2148-9-160"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.pt.2010.12.001","article-title":"Quantitative genetics of Aedes aegypti vector competence for dengue viruses: Towards a new paradigm?","volume":"27","author":"Lambrechts","year":"2011","journal-title":"Trends Parasitol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Lequime, S., Fontaine, A., Gouilh, M.A., Moltini-Conclois, I., and Lambrechts, L. (2016). Genetic drift, purifying selection and vector genotype shape dengue virus intra-host genetic diversity in mosquitoes. PLoS Genet., 12.","DOI":"10.1371\/journal.pgen.1006111"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"85","DOI":"10.4269\/ajtmh.2002.67.85","article-title":"Variation in vector competence for dengue 2 virus among 24 collections of Aedes aegypti from Mexico and the United States","volume":"67","author":"Bennett","year":"2002","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"445","DOI":"10.4269\/ajtmh.2005.73.445","article-title":"Potential role of sylvatic and domestic African mosquito species in dengue emergence","volume":"73","author":"Diallo","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s00239-002-2360-y","article-title":"Geographic genetic variation in populations of the dengue virus vector Aedes aegypti","volume":"55","author":"Failloux","year":"2002","journal-title":"J. Mol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Goncalves, C.M., Melo, F.F., Bezerra, M.T., Chaves, B.A., Silva, B.M., Silva, L.D., Pessanha, J.E.M., Arias, J.R., Secundino, N.F.C., and Norris, D.E. (2014). Distinct variation in vector competence among nine field populations of Aedes aegypti from a Brazilian dengue-endemic risk city. Parasites Vectors.","DOI":"10.1186\/1756-3305-7-320"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.4269\/ajtmh.1979.28.1045","article-title":"Variation in susceptibility to oral infection with dengue viruses among geographic strains of Aedes aegypti","volume":"28","author":"Gubler","year":"1979","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/j.1365-2915.2007.00699.x","article-title":"Investigations of dengue-2 susceptibility and body size among Aedes aegypti populations","volume":"21","author":"Schneider","year":"2007","journal-title":"Med. Vet. Entomol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4269\/ajtmh.1998.58.283","article-title":"Effect of size and geographic origin of Aedes aegypti on oral infection with dengue-2 virus","volume":"58","author":"Sumanochitrapon","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"308","DOI":"10.4269\/ajtmh.1990.43.308","article-title":"Variation among strains of Aedes aegypti in susceptibility to oral infection with denge virus type 2","volume":"43","author":"Tardieux","year":"1990","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"886","DOI":"10.4269\/ajtmh.2006.75.886","article-title":"Aedes aegypti vectorial capacity is determined by the infecting genotype of the dengue virus","volume":"75","author":"Anderson","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"539","DOI":"10.4269\/ajtmh.2003.68.539","article-title":"Efficiency of dengue serotype 2 virus strains to infect and disseminate in Aedes aegypti","volume":"68","author":"Armstrong","year":"2003","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Fernandes da Moura, A.J., Varjal de Melo Santos, M.A., Oliveira, C.M.F., Guedes, D.R.D.G., de Carvalho-Leandro, D., da Cruz Brito, M.L., Rocha, H.D.R., Gomez, L.F., and Ayres, C.F.J. (2015). Vector competence of the Aedes aegypti population from Santiago Island, Cape Verde, to different serotypes of dengue virus. Parasites Vectors.","DOI":"10.1186\/s13071-015-0706-8"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1111\/tmi.12373","article-title":"Oral susceptibility of Aedes aegypti (Diptera: Culicidae) from Senegal for dengue serotypes 1 and 3 viruses","volume":"19","author":"Gaye","year":"2014","journal-title":"Trop. Med. Int. Health"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Khoo, C.C.H., Doty, J.B., Held, N.L., Olson, K.E., and Franz, A.W.E. (2013). Isolation of midgut escape mutants of two American genotype dengue 2 viruses from Aedes aegypti. Virol. J.","DOI":"10.1186\/1743-422X-10-257"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1128\/JVI.06458-11","article-title":"Dengue-1 virus clade replacement in Thailand associated with enhanced mosquito transmission","volume":"86","author":"Lambrechts","year":"2012","journal-title":"J. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"689","DOI":"10.4269\/ajtmh.12-0488","article-title":"Reduction of Aedes aegypti vector competence for dengue virus under large temperature fluctuations","volume":"88","author":"Carrington","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1017\/S0016672303006463","article-title":"Variation over space and time of Aedes aegypti in Phnom Penh (Cambodia): Genetic structure and oral susceptibility to a dengue virus","volume":"82","author":"Paupy","year":"2003","journal-title":"Genet. Res. Camb."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Vazeille, M., Gaborit, P., Mousson, L., Girod, R., and Failloux, A.-B. (2016). Competitive advantage of a dengue 4 virus when co-infecting the mosquito Aedes aegypti with dengue 1 virus. BMC Infect. Dis.","DOI":"10.1186\/s12879-016-1666-0"},{"key":"ref_39","unstructured":"Marquardt, W.C. (2005). Biology of Disease Vectors, Elsevier. [2nd ed.]."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Smith, D.L., Battle, K.E., Hay, S.I., Barker, C.M., Scott, T.W., and McKensie, F.E. (2012). Ross, Macdonald, and a theory for the dynamics and control of mosquito-transmitted pathogens. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002588"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.coviro.2016.09.008","article-title":"Ecological effects on arbovirus-mosquito cycles of transmission","volume":"21","author":"Tabachnick","year":"2016","journal-title":"Curr. Opin. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/j.1365-2915.2007.00694.x","article-title":"Body size-associated survival and dispersal rates of Aedes aegypti in Rio de Janeiro","volume":"21","author":"Codeco","year":"2007","journal-title":"Med. Vet. Entomol."},{"key":"ref_43","first-page":"61","article-title":"The size of emerging and host-seeking Aedes aegypti and the relationship of size to blood-feeding success in the field","volume":"2","author":"Nasci","year":"1986","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1093\/jmedent\/28.4.522","article-title":"Influence of larval and adult nutrition on biting persistence in Aedes aegypti (Diptera: Culicidae)","volume":"31","author":"Nasci","year":"1991","journal-title":"J. Med. Entomol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"441","DOI":"10.2307\/2411116","article-title":"Costs and benefits of mosquito refractoriness to malaria parasites: Implications for genetic variability of mosquitoes and genetic control of malaria","volume":"51","author":"Yan","year":"1997","journal-title":"Evolution"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1111\/mec.12771","article-title":"Mosquitoes rely on their gut microbiota for development","volume":"23","author":"Coon","year":"2014","journal-title":"Mol. Ecol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Coon, K.L., Brown, M.R., and Strand, M.R. (2016). Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae). Parasit. Vectors.","DOI":"10.1186\/s13071-016-1660-9"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1603\/0022-2585-37.1.89","article-title":"Longitudinal studies of Aedes aegypti (Diptera: Culicidae) in Thailand and Puerto Rico: Blood feeding frequency","volume":"37","author":"Scott","year":"2000","journal-title":"J. Med. Entomol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.meegid.2010.10.019","article-title":"Heritability and adaptive phenotypic plasticity of adult body size in the mosquito Aedes aegypti with implications for dengue vector competence","volume":"11","author":"Schneider","year":"2011","journal-title":"Infect. Genet. Evol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1093\/jmedent\/43.3.484","article-title":"Ecological factors influencing Aedes aegypti (Diptera: Culicidae) productivity in artificial containers in Salinas, Puerto Rico","volume":"43","author":"Barrera","year":"2006","journal-title":"J. Med. Entomol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.actatropica.2009.06.008","article-title":"Environmental conditions in water storage drums and influences on Aedes aegypti in Trinidad, West Indies","volume":"112","author":"Hemme","year":"2009","journal-title":"Acta Trop."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"53","DOI":"10.7601\/mez.53.53_1","article-title":"Effect of temperature on the development of Aedes aegypti and Aedes albopictus","volume":"53","author":"Kamimura","year":"2002","journal-title":"Med. Entomol. Zool."},{"key":"ref_53","first-page":"338","article-title":"Effect of food and temperature on Aedes aegypti (L.) and Aedes triseriatus (SAY) larval development","volume":"28","author":"Keirans","year":"1968","journal-title":"Mosq. News"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1038\/ni1237","article-title":"The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of drosophila","volume":"6","author":"Dostert","year":"2005","journal-title":"Nat. Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1744-7917.2008.00185.x","article-title":"Innate immunity in Drosophila: Pathogens and pathways","volume":"15","author":"Govind","year":"2008","journal-title":"Insect Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1146\/annurev.immunol.25.022106.141615","article-title":"The host defense of Drosophila melanogaster","volume":"25","author":"Lemaitre","year":"2007","journal-title":"Annu. Rev. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/j.1600-065X.2010.00985.x","article-title":"Intracellular immune responses of dipteran insects","volume":"240","author":"Steinert","year":"2011","journal-title":"Immunol. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/S1369-5274(02)00294-1","article-title":"How Drosophila combats microbial infection: A model to study innate immunity and host-pathogen interactions","volume":"5","author":"Tzou","year":"2002","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1126\/science.1139862","article-title":"Evolutionary dynamics of immune-related genes and pathways in disease-vector mosquitoes","volume":"316","author":"Waterhouse","year":"2007","journal-title":"Science"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1111\/j.1462-5822.2011.01649.x","article-title":"Innate immune control of West Nile virus infection","volume":"13","author":"Arjona","year":"2011","journal-title":"Cell. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.dci.2009.12.005","article-title":"Mosquito immune defenses against Plasmodium infection","volume":"34","author":"Cirimotich","year":"2010","journal-title":"Dev. Comp. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0952-7915(00)00186-2","article-title":"Innate immune defense against malaria infection in the mosquito","volume":"13","author":"Dimopoulos","year":"2001","journal-title":"Curr. Opin. Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1099\/vir.0.013201-0","article-title":"Advances in dissecting mosquito innate immune responses to arbovirus infection","volume":"90","author":"Fragkoudis","year":"2009","journal-title":"J. Gen. Virol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4921","DOI":"10.1016\/j.jmb.2013.10.006","article-title":"Insect antiviral innate immunity: Pathways, effectors and connections","volume":"425","author":"Kingsolver","year":"2013","journal-title":"J. Mol. Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3142","DOI":"10.3390\/v5123142","article-title":"The role of innate immunity in conditioning mosquito susceptibility to West Nile Virus","volume":"5","author":"Prasad","year":"2013","journal-title":"Viruses"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4479","DOI":"10.3390\/v6114479","article-title":"Mosquito immunity against arboviruses","volume":"6","author":"Sim","year":"2014","journal-title":"Viruses"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/S0188-4409(02)00373-9","article-title":"Flavivirus susceptibility in Aedes aegypti","volume":"33","author":"Black","year":"2002","journal-title":"Arch. Med. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1146\/annurev.ento.46.1.183","article-title":"Genetic and physical mapping in mosquitoes: Molecular approaches","volume":"46","author":"Severson","year":"2001","journal-title":"Ann. Rev. Entomol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"965","DOI":"10.4269\/ajtmh.1998.59.965","article-title":"Quantitative genetics of vector competence for dengue-2 virus in Aedes aegypti","volume":"59","author":"Bosio","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1534\/genetics.104.035634","article-title":"Quantitative trait loci that control dengue-2 virus dissemination in the mosquito Aedes aegypti","volume":"170","author":"Bennett","year":"2005","journal-title":"Genetics"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1093\/genetics\/156.2.687","article-title":"Quantative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti","volume":"156","author":"Bosio","year":"2000","journal-title":"Genetics"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1111\/j.0962-1075.2004.00522.x","article-title":"Quantitative trait loci affecting dengue midgut infection barriers in an advanced intercross line of Aedes aegypti","volume":"13","author":"Bennett","year":"2004","journal-title":"Insect Mol. Biol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1006\/expr.1995.1126","article-title":"Aedes aegypti: A quantitative trait locus (QTL) influencing filarial worm intensity is linked to QTL for susceptibility to other mosquito-borne pathogens","volume":"81","author":"Beerntsen","year":"1995","journal-title":"Exp. Parasitol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1365-2583.1994.tb00153.x","article-title":"Chromosomal mapping of two loci affecting filarial worm susceptibility in Aedes aegypti","volume":"3","author":"Severson","year":"1994","journal-title":"Insect Mol. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1093\/genetics\/139.4.1711","article-title":"Restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito Aedes aegypti","volume":"139","author":"Severson","year":"1995","journal-title":"Genetics"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1534\/genetics.105.055178","article-title":"Amplified fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the yellow fever mosquito Aedes aegypti","volume":"173","author":"Zhong","year":"2006","journal-title":"Genetics"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Timoshevskiy, V.A., Severson, D.W., deBruyn, B.S., Black, W.C., Sharakhov, I.V., and Sharakhova, M.V. (2013). An integrated linkage, chromosome, and genome map for the yellow fever mosquito Aedes aegypti. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002052"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1094\/PHYTO-96-0120","article-title":"The genetic architecture of disease resistance in maize: A synthesis of published studies","volume":"96","author":"Wisser","year":"2006","journal-title":"Phytopathology"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2773","DOI":"10.1007\/s00018-010-0357-z","article-title":"Dengue virus life cycle: Viral and host factors modulating infectivity","volume":"67","author":"Wilschut","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2583.2011.01098.x","article-title":"An update on mosquito cell expressed dengue virus receptor proteins","volume":"21","author":"Smith","year":"2012","journal-title":"Insect Mol. Biol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1099\/vir.0.83357-0","article-title":"Functional entry of dengue virus into Aedes albopictus mosquito cells is dependent on clathrin-mediated endocytosis","volume":"89","author":"Acosta","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.virol.2008.05.012","article-title":"Endocytic pathway followed by dengue virus to infect the mosquito cell line C6\/36 HT","volume":"378","author":"Mosso","year":"2008","journal-title":"Virology"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4269\/ajtmh.2006.74.132","article-title":"Quantitative analysis of dengue-2 virus RNA during the extrinsic incubation period in individual Aedes aegypti","volume":"74","author":"Richardson","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Salazar, M.I., Richardson, J.H., Sanchez-Vargas, I., Olson, K.E., and Beaty, B.J. (2007). Dengue virus type 2: Replication and tropisms in orally infected Aedes aegypti mosquitoes. BMC Microbiol.","DOI":"10.1186\/1471-2180-7-9"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1093\/jmedent\/30.2.326","article-title":"Midgut basal lamina thickness and dengue-1 virus dissemination rates in laboratory strains of Aedes albopictus (Diptera: Culicidae)","volume":"30","author":"Thomas","year":"1993","journal-title":"J. Med. Entomol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"9072","DOI":"10.1073\/pnas.1303395110","article-title":"Host and viral features of human dengue cases shape the population of infected and infectious Aedes aegypti mosquitoes","volume":"110","author":"Nguyet","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"422","DOI":"10.4269\/ajtmh.13-0186","article-title":"Comparative susceptibility of mosquito populations in North Queensland, Australia to oral infection with dengue virus","volume":"90","author":"Ye","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Ye, Y.H., Chenoweth, S.F., Carrasco, A.M., Allen, S.L., Frentiu, F.D., van den Hurk, A.F., Beebe, N.W., and McGraw, E.A. (2016). Evolutionary potential of the extrinsic incubation period of dengue virus in Aedes aegypti. Evolution.","DOI":"10.1111\/evo.13039"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1126\/science.1138878","article-title":"Genome sequence of Aedes aegypti, a major arbovirus vector","volume":"316","author":"Nene","year":"2007","journal-title":"Science"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Xi, Z., Ramirez, J.L., and Dimopoulos, G. (2008). The Aedes aegypti Toll pathway controls dengue virus infection. PLoS Pathog., 4.","DOI":"10.1371\/journal.ppat.1000098"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"17841","DOI":"10.1073\/pnas.0905006106","article-title":"An evolutionary conserved function of the JAK-STAT pathway in anti-dengue defense","volume":"106","author":"Sim","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Colpitts, T.M., Cox, J., Vanlandingham, D.L., Feitosa, F.M., Cheng, G., Kurscheid, S., Wang, P., Kishnan, M.N., Higgs, S., and Fikrig, E. (2011). Alterations in the Aedes aegypti transcriptome during infection with West Nile, dengue and yellow fever viruses. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002189"},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Bonizzoni, M., Dunn, W.A., Campbell, C.L., Olson, K.E., Marinotti, O., and James, A.A. (2012). Complex modulation of the Aedes aegypti transcriptome in response to dengue virus infection. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0050512"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Sim, S., Ramirez, J.L., and Dimopoulos, G. (2012). Dengue virus infection of the Aedes aegypti salivary gland and chemosensary apparatus induces genes that modulate infection and blood-feeding behavior. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002631"},{"key":"ref_95","unstructured":"Baron, O.L., Ursic-Bedoya, R.J., Lowenberger, C.A., and Ocampo, C.B. (2008). Differential gene expression from midguts of refractory and susceptible lines of the mosquito, Aedes aegypti, infected with dengue-2 virus. J. Insect Sci."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Behura, S.K., Gomez-Machorro, C., Harker, B.W., deBruyn, B., Lovin, D.D., Hemme, R.R., Mori, A., Romero-Severson, J., and Severson, D.W. (2011). Global cross-talk of genes of the mosquito Aedes aegypti in response to dengue virus infection. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001385"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1007\/s10142-014-0376-1","article-title":"Influence of mosquito genotype on transcriptional response to dengue virus infection","volume":"14","author":"Behura","year":"2014","journal-title":"Funct. Integr. Genom."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Chauhan, C., Behura, S.K., deBruyn, B., Lovin, D.D., Harker, B.W., Gomez-Machorro, C., Mori, A., Romero-Severson, J., and Severson, D.W. (2012). Comparative expression profiles of midgut genes in dengue virus refractory and susceptible Aedes aegypti across critical period for virus infection. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047350"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Sim, S., Jupatanakul, N., Ramirez, J.L., Kang, S., Romero-Vivas, C.M., Mohammed, H., and Dimopoulos, G. (2013). Transcriptomic profiling of diverse Aedes aegypti strains reveals increased basal-level immune activation in dengue virus-refractory populations and identifies novel virus-vector molecular interactions. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002295"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.pt.2015.09.009","article-title":"Mosquito defense strategies against viral infection","volume":"32","author":"Cheng","year":"2016","journal-title":"Trends Parasitol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1099\/jgv.0.000429","article-title":"Arboviruses and apoptosis: The role of cell death in determining vector competence","volume":"97","author":"Clem","year":"2016","journal-title":"J. Gen. Virol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.coviro.2015.10.001","article-title":"Arbovirus-mosquito interactions: RNAi pathway","volume":"15","author":"Olson","year":"2015","journal-title":"Curr. Opin. Virol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"19938","DOI":"10.1073\/pnas.0803408105","article-title":"Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes","volume":"105","author":"Myles","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1016\/j.jmb.2013.11.023","article-title":"Innate immunity to dengue virus infection and subversion of antiviral responses","volume":"426","author":"Green","year":"2014","journal-title":"J. Mol. Biol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.meegid.2014.02.010","article-title":"The damage threshold hypothesis and the immune strategies of insects","volume":"24","author":"Conde","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev-immunol-020711-075030","article-title":"Tolerance of infections","volume":"30","author":"Ayres","year":"2012","journal-title":"Annu. Rev. Immunol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.ibmb.2016.07.004","article-title":"Apoptosis-related genes control autophagy and influence DENV-2 infection in the mosquito vector, Aedes aegypti","volume":"76","author":"Eng","year":"2016","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.micpath.2014.03.004","article-title":"Role of intracellular events in the pathogenesis of dengue: An overview","volume":"69\u201370","author":"Jain","year":"2014","journal-title":"Microb. Pathog."},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Lobo, F.P., Mota, B.E.F., Pena, S.D.J., Azevedo, V., Macedo, A.M., Tauch, A., Machado, C.R., and Franco, G.R. (2009). Virus-host coevolution: Common patterns of nucleotide motif usage in Flaviviridae and their hosts. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006282"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.tig.2007.04.004","article-title":"A call to arms: Coevolution of animal viruses and host innate immune responses","volume":"23","author":"Marques","year":"2007","journal-title":"Trends Genet."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.mib.2016.05.002","article-title":"The diversity of insect antiviral immunity: Insights from viruses","volume":"32","author":"Marques","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/j.meegid.2012.04.027","article-title":"Intrinsic features of Aedes aegypti genes affect transcriptional responsiveness of mosquito genes to dengue virus infection","volume":"12","author":"Behura","year":"2012","journal-title":"Infect. Genet. Evol."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Calvo, E., Pham, V.M., Marinotti, O., Andersen, J.F., and Ribeiro, J.M. (2009). The salivary gland transcriptome of the neotropical malaria vector Anopheles darlingi reveals accelerated evolution of genes relevant to hematophagy. BMC Genom.","DOI":"10.1186\/1471-2164-10-57"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/molbev\/msh010","article-title":"Mammalian housekeeping genes evolve more slowly than tissue-specific genes","volume":"21","author":"Zhang","year":"2004","journal-title":"Mol. Biol. Evol."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Bryant, C.E., and Monie, T.P. (2012). Mice, men and the relatives: Cross-species studies underpin innate immunity. Open Biol.","DOI":"10.1098\/rsob.120015"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.coviro.2014.07.002","article-title":"The genetics of host-virus coevolution in invertebrates","volume":"8","author":"Obbard","year":"2014","journal-title":"Curr. Opin. Virol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1111\/eva.12360","article-title":"No evidence for local adaptation of dengue viruses to mosquito vector populations in Thailand","volume":"9","author":"Fransiri","year":"2016","journal-title":"Evol. Appl."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Behura, S.K., Sarro, J., Li, P., Mysore, K., Severson, D.W., Emrich, S.J., and Duman-Scheel, M. (2016). High-throughput cis-regulatory element discovery in the vector mosquito Aedes aegypti. BMC Genom.","DOI":"10.1186\/s12864-016-2468-x"},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Behura, S.K., and Severson, D.W. (2013). Nucleotide substitutions in dengue virus serotypes from Asian and American countries: Insights into intracodon recombination and purifying selection. BMC Microbiol.","DOI":"10.1186\/1471-2180-13-37"},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Lara-Ramirez, E.E., Salazar, M.I., Lopez-Lopez, M.J., Salas-Benito, J.S., Sanchez-Varela, A., and Guo, X. (2014). Large-scale genomic analysis of codon usage in dengue virus and evaluation of its phylogenetic dependence. Biomed. Res. Int.","DOI":"10.1155\/2014\/851425"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1007\/s00438-014-0857-x","article-title":"Bicluster pattern of codon context usages between flavivirus and vector mosquito Aedes aegypti: Relevance to infection and transcriptome response of mosquito genes","volume":"289","author":"Behura","year":"2014","journal-title":"Mol. Genet. Genom."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"4749","DOI":"10.1073\/pnas.1502864112","article-title":"Large-scale recoding of an arbovirus genome to rebalance its insect versus mammalian preference","volume":"112","author":"Shen","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Vasilakis, N., Deardorff, E.R., Kenney, J.L., Rossi, S.L., Hanley, K.A., and Weaver, S.C. (2009). Mosquitoes put the brake on arbovirus evolution: Experimental evolution reveals slower mutation accumulation in mosquito than vertebrate cells. PLoS Pathog.","DOI":"10.1371\/journal.ppat.1000467"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-6851(02)00028-2","article-title":"Development and applications of transgenesis in the yellow fever mosquito, Aedes aegypti","volume":"121","author":"Adelman","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_125","doi-asserted-by":"crossref","unstructured":"Burt, B. (2014). Heritable strategies for controlling insect vectors of disease. Philos. Trans. R. Soc. B.","DOI":"10.1098\/rstb.2013.0432"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1016\/S0020-7519(02)00188-1","article-title":"Genetic transformation of mosquitoes: A quest for malaria control","volume":"32","author":"Moreira","year":"2002","journal-title":"Int. J. Parasitol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ibmb.2003.10.004","article-title":"Insect transgenesis and its potential role in agriculture and human health","volume":"34","author":"Robinson","year":"2004","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.pt.2008.05.002","article-title":"Flipping the paradigm on malaria transmission-blocking vaccines","volume":"24","author":"Dinglasan","year":"2008","journal-title":"Trends Parasitol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.chom.2008.09.002","article-title":"Molecular genetic manipulation of vector mosquitoes","volume":"4","author":"Terenius","year":"2014","journal-title":"Cell Host Microbe"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1126\/science.298.5591.117","article-title":"The ecology of genetically modified mosquitoes","volume":"298","author":"Scott","year":"2002","journal-title":"Science"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/nrmicro2900","article-title":"Rethinking vector immunology: The role of environmental temperature in shaping resistance","volume":"10","author":"Murdock","year":"2012","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/7\/4\/58\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:34:21Z","timestamp":1760211261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/7\/4\/58"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,30]]},"references-count":131,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2016,12]]}},"alternative-id":["insects7040058"],"URL":"https:\/\/doi.org\/10.3390\/insects7040058","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,30]]}}}