{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T19:15:50Z","timestamp":1772133350523,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,4]],"date-time":"2023-06-04T00:00:00Z","timestamp":1685836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2018YFA0801005"],"award-info":[{"award-number":["2018YFA0801005"]}]},{"name":"National Key R&amp;D Program of China","award":["2018YFA0800500"],"award-info":[{"award-number":["2018YFA0800500"]}]},{"name":"National Key R&amp;D Program of China","award":["SN-ZJU-SIAS-004"],"award-info":[{"award-number":["SN-ZJU-SIAS-004"]}]},{"name":"Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study","award":["2018YFA0801005"],"award-info":[{"award-number":["2018YFA0801005"]}]},{"name":"Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study","award":["2018YFA0800500"],"award-info":[{"award-number":["2018YFA0800500"]}]},{"name":"Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study","award":["SN-ZJU-SIAS-004"],"award-info":[{"award-number":["SN-ZJU-SIAS-004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCDD"],"abstract":"<jats:p>The heart switches its main metabolic substrate from glucose to fatty acids shortly after birth, which is one of reasons for the loss of heart regeneration capability in adult mammals. On the contrary, metabolic shifts from oxidative phosphorylation to glucose metabolism promote cardiomyocyte (CM) proliferation after heart injury. However, how glucose transportation in CMs is regulated during heart regeneration is still not fully understood. In this report, we found that the expression of Glut1 (slc2a1) was upregulated around the injury site of zebrafish heart, accompanied by an increase in glucose uptake at the injury area. Knockout of slc2a1a impaired zebrafish heart regeneration. Our previous study has demonstrated that the expression of \u0394113p53 is activated after heart injury and \u0394113p53+ CMs undergo proliferation to contribute to zebrafish heart regeneration. Next, we used the \u0394113p53 promoter to generate the Tg(\u0394113p53:cmyc) zebrafish transgenic line. Conditional overexpression of cmyc not only significantly promoted zebrafish CM proliferation and heart regeneration but also significantly enhanced glut1 expression at the injury site. Inhibiting Glut1 diminished the increase in CM proliferation in Tg(\u0394113p53:cmyc) injured hearts of zebrafish. Therefore, our results suggest that the activation of cmyc promotes heart regeneration through upregulating the expression of glut1 to speed up glucose transportation.<\/jats:p>","DOI":"10.3390\/jcdd10060246","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T02:18:29Z","timestamp":1685931509000},"page":"246","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tg(\u0394113p53:cmyc) Transgene Upregulates glut1 Expression to Promote Zebrafish Heart Regeneration"],"prefix":"10.3390","volume":"10","author":[{"given":"Zimu","family":"Tang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Biosystems Homeostasis & Protection, College of Life Sciences, Cancer Center, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Biosystems Homeostasis & Protection, College of Life Sciences, Cancer Center, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Lo","sequence":"additional","affiliation":[{"name":"College of Animal Sciences, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4459-8804","authenticated-orcid":false,"given":"Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Biosystems Homeostasis & Protection, College of Life Sciences, Cancer Center, Zhejiang University, Hangzhou 310058, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1016\/j.jacc.2020.11.010","article-title":"Global Burden of Cardiovascular Diseases and Risk Factors, 1990\u20132019: Update From the GBD 2019 Study","volume":"76","author":"Roth","year":"2020","journal-title":"J. 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