{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:17:40Z","timestamp":1760239060696,"version":"build-2065373602"},"reference-count":58,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T00:00:00Z","timestamp":1601683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R15 HD084262-01"],"award-info":[{"award-number":["R15 HD084262-01"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCDD"],"abstract":"<jats:p>In the last two decades, the zebrafish has emerged as an important model species for heart regeneration studies. Various approaches to model loss of cardiac myocytes and myocardial infarction in the zebrafish have been devised, and have included resection, genetic ablation, and cryoinjury. However, to date, the response of the zebrafish ventricle to cautery injury has not been reported. Here, we describe a simple and reproducible method using cautery injury via a modified nichrome inoculating needle as a probe to model myocardial infarction in the zebrafish ventricle. Using light and electron microscopy, we show that cardiac cautery injury is attended by significant inflammatory cell infiltration, accumulation of collagen in the injured area, and the reconstitution of the ventricular myocardium. Additionally, we document the ablation of cardiac nerve fibers, and report that the re-innervation of the injured zebrafish ventricle is protracted, compared to other repair processes that accompany the regeneration of the cauterized ventricle. Taken together, our study demonstrates that cautery injury is a simple and effective means for generating necrotic tissue and eliciting a remodeling and regenerative response in the zebrafish heart. This approach may serve as an important tool in the methods toolbox for regeneration studies in the zebrafish.<\/jats:p>","DOI":"10.3390\/jcdd7040041","type":"journal-article","created":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T07:22:16Z","timestamp":1601709736000},"page":"41","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cauterization as a Simple Method for Regeneration Studies in the Zebrafish Heart"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2998-9508","authenticated-orcid":false,"given":"Papa K. Van","family":"Dyck","sequence":"first","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Hockaden","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma C.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa R.","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamil L.","family":"Hester","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1277-5797","authenticated-orcid":false,"given":"Neil","family":"Pillai","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8546-6595","authenticated-orcid":false,"given":"Gabrielle C.","family":"Coffing","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Burns","sequence":"additional","affiliation":[{"name":"College of Optometry, University of Houston, Houston, TX 77204, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7230-6883","authenticated-orcid":false,"given":"Pascal J.","family":"Lafontant","sequence":"additional","affiliation":[{"name":"Department of Biology, DePauw University, Greencastle, IN 46135, USA"},{"name":"Buehler Biomedical Imaging Center, DePauw University, Greencastle, IN 46135, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ritchey, M.D., Wall, H.K., George, M.G., and Wright, J.S. 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