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However, no pharmacological treatment is currently approved to prevent the outbreak of NASH, which leads to fibrosis and cirrhosis. Preclinical research is required to improve our knowledge of NAFLD physiopathology and to identify new therapeutic targets. In the present review, we summarize advances in NAFLD preclinical models from cellular models, including new bioengineered platforms, to in vivo models, with a particular focus on genetic and dietary mouse models. We aim to discuss the advantages and limits of these different models.<\/jats:p>","DOI":"10.3390\/jcm10010036","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T22:56:45Z","timestamp":1608850605000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":115,"title":["In Vitro and In Vivo Models of Non-Alcoholic Fatty Liver Disease: A Critical Appraisal"],"prefix":"10.3390","volume":"10","author":[{"given":"Pierre-Antoine","family":"Soret","sequence":"first","affiliation":[{"name":"Centre de Recherche Saint-Antoine (CRSA), Sorbonne Universit\u00e9, Inserm, 75012 Paris, France"},{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Hepatology Department, Reference Center for Inflammatory Biliary Diseases and Autoimmune Hepatitis, Saint-Antoine Hospital, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Magusto","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine (CRSA), Sorbonne Universit\u00e9, Inserm, 75012 Paris, France"},{"name":"Institute of Cardiometabolism and Nutrition (ICAN), Sorbonne Universit\u00e9, Inserm, AP-HP, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Housset","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine (CRSA), Sorbonne Universit\u00e9, Inserm, 75012 Paris, France"},{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Hepatology Department, Reference Center for Inflammatory Biliary Diseases and Autoimmune Hepatitis, Saint-Antoine Hospital, 75012 Paris, France"},{"name":"Institute of Cardiometabolism and Nutrition (ICAN), Sorbonne Universit\u00e9, Inserm, AP-HP, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9mie","family":"Gautheron","sequence":"additional","affiliation":[{"name":"Centre de Recherche Saint-Antoine (CRSA), Sorbonne Universit\u00e9, Inserm, 75012 Paris, France"},{"name":"Institute of Cardiometabolism and Nutrition (ICAN), Sorbonne Universit\u00e9, Inserm, AP-HP, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"European Association for the Study of the Liver (EASL), European Association for the Study of Diabetes (EASD), and European Association for the Study of Obesity (EASO) (2016). 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