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This work analyzed overexpressed sequences from the cercaria phase to the early schistosomulum phase using bioinformatics tools to predict host interaction and selected proteins for predicting T cell epitopes. The final peptides were chemically synthesized, and their toxicity was evaluated in vitro. Peptides were formulated in the Adjuvant Adaptation (ADAD) vaccination system and injected into BALB\/c mice that were challenged with S. mansoni cercariae to assess protection and immunogenicity. A total of 39 highly expressed S.mansoni proteins were identified as being of potential interest. Three T cell peptides predicted to bind MHC mouse and human class II were synthesized and formulated for vaccination. SmGSP and SmIKE reduced the number of eggs trapped in the liver by more than 50% in challenged BALB\/c mice. The liver of mice vaccinated with either SmGSP or SmTNP had a significantly reduced affected liver surface. Transcriptome-based T cell peptides elicit partial protection and could be candidates for a multiantigen vaccine.<\/jats:p>","DOI":"10.3390\/jcm10030445","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T02:07:00Z","timestamp":1611540420000},"page":"445","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["T Cell Peptides Derived from Invasive Stages of Schistosoma mansoni as Potential Schistosomiasis Vaccine"],"prefix":"10.3390","volume":"10","author":[{"given":"Julio","family":"L\u00f3pez-Ab\u00e1n","sequence":"first","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"Vicente","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6290-9493","authenticated-orcid":false,"given":"El\u00edas","family":"Kabbas-Pi\u00f1ango","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Hern\u00e1ndez-Goenaga","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4227-9834","authenticated-orcid":false,"given":"Javier","family":"S\u00e1nchez-Montejo","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Aguiriano","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"del Olmo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, IBSAL-CIETUS, Faculty of Pharmacy, University of Salamanca, Ldo M\u00e9ndez Nieto s\/n, 37007 Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnolia","family":"Vanegas","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Department, Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Carrera 50#26-20, Bogot\u00e1 DC 111321, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-2500","authenticated-orcid":false,"given":"Manuel Alfonso","family":"Patarroyo","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Department, Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Carrera 50#26-20, Bogot\u00e1 DC 111321, Colombia"},{"name":"Microbiology Department, Faculty of Medicine, Universidad Nacional de Colombia, Carrera 45#26-85, Bogot\u00e1 DC 111321, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0244-4740","authenticated-orcid":false,"given":"Antonio","family":"Muro","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Group (e-INTRO), IBSAL-CIETUS (Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca), Faculty of Pharmacy, University of 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2017","volume":"392","author":"Roth","year":"2018","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1016\/S0140-6736(18)32335-3","article-title":"Global, regional, and national disability-adjusted life-years (DALYs) for 359 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990\u20132017: A systematic analysis for the Global Burden of Disease Study 2017","volume":"392","author":"Kyu","year":"2018","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e42","DOI":"10.1016\/S1473-3099(16)30475-3","article-title":"Human schistosomiasis in the post mass drug administration era","volume":"17","author":"Mutapi","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Riveau, G., Schacht, A.M., Dompnier, J.P., Deplanque, D., Seck, M., Waucquier, N., Senghor, S., Delcroix-Genete, D., Hermann, E., and Idris-Khodja, N. (2018). Safety and efficacy of the rSh28GST urinary schistosomiasis vaccine: A phase 3 randomized, controlled trial in Senegalese children. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006968"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Riveau, G., Deplanque, D., Remou\u00e9, F., Schacht, A.M., Vodougnon, H., Capron, M., Thiry, M., Martial, J., Libersa, C., and Capron, A. (2012). Safety and immunogenicity of rSh28GST antigen in humans: Phase 1 randomized clinical study of a vaccine candidate against urinary schistosomiasis. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001704"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-016-1799-4","article-title":"Schistosomiasis vaccines: Where do we stand?","volume":"9","author":"Tebeje","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.immuni.2010.09.017","article-title":"Reverse vaccinology: Developing vaccines in the era of genomics","volume":"33","author":"Sette","year":"2010","journal-title":"Immunity"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.2174\/138161209789105171","article-title":"HLA-DR: Molecular Insights and Vaccine Design","volume":"15","author":"Stern","year":"2009","journal-title":"Curr. Pharm. Des."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Picard, M.A.L., Boissier, J., Roquis, D., Grunau, C., Allienne, J.F., Duval, D., Toulza, E., Arancibia, N., Caffrey, C.R., and Long, T. (2016). Sex-Biased Transcriptome of Schistosoma mansoni: Host-Parasite Interaction, Genetic Determinants and Epigenetic Regulators Are Associated with Sexual Differentiation. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004930"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"156","DOI":"10.3389\/fcimb.2018.00156","article-title":"The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile","volume":"8","author":"Patarroyo","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev-immunol-032712-095910","article-title":"Pathways of antigen processing","volume":"31","author":"Blum","year":"2013","journal-title":"Annu. Rev. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Rojas-Caraballo, J., L\u00f3pez-Ab\u00e1n, J., P\u00e9rez Del Villar, L., Vizca\u00edno, C., Vicente, B., Fern\u00e1ndez-Soto, P., Del Olmo, E., Patarroyo, M.A., and Muro, A. (2014). In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105323"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/nature08160","article-title":"The genome of the blood fluke Schistosoma mansoni","volume":"460","author":"Berriman","year":"2009","journal-title":"Nature"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nature08140","article-title":"The Schistosoma japonicum genome reveals features of host-parasite interplay","volume":"460","author":"Zhou","year":"2009","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/ng.1065","article-title":"Whole-genome sequence of Schistosoma haematobium","volume":"44","author":"Young","year":"2012","journal-title":"Nat. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"20954","DOI":"10.1038\/srep20954","article-title":"Whole genome resequencing of the human parasite Schistosoma mansoni reveals population history and effects of selection","volume":"6","author":"Crellen","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Protasio, A.V., Tsai, I.J., Babbage, A., Nichol, S., Hunt, M., Aslett, M.A., de Silva, N., Velarde, G.S., Anderson, T.J.C., and Clark, R.C. (2012). A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001455"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gobert, G.N., Tran, M.H., Moertel, L., Mulvenna, J., Jones, M.K., McManus, D.P., and Loukas, A. (2010). Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000600"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Anderson, L., Amaral, M.S., Beckedorff, F., Silva, L.F., Dazzani, B., Oliveira, K.C., Almeida, G.T., Gomes, M.R., Pires, D.S., and Setubal, J.C. (2015). Schistosoma mansoni Egg, Adult Male and Female Comparative Gene Expression Analysis and Identification of Novel Genes by RNA-Seq. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0004334"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/978-1-4939-7015-5_6","article-title":"Predicting secretory proteins with signaIP","volume":"1611","author":"Nielsen","year":"2017","journal-title":"Methods Mol Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ijpara.2016.11.007","article-title":"The genome of the protozoan parasite Cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates","volume":"47","author":"Palmieri","year":"2017","journal-title":"Int. J. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Saha, S., and Raghava, G.P.S. (2006). AlgPred: Prediction of allergenic proteins and mapping of IgE epitopes. Nucleic Acids Res., 34.","DOI":"10.1093\/nar\/gkl343"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s002510050595","article-title":"SYFPEITHI: Database for MHC ligands and peptide motifs","volume":"50","author":"Rammensee","year":"1999","journal-title":"Immunogenetics"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nielsen, M., and Lund, O. (2009). NN-align. An artificial neural network-based alignment algorithm for MHC class II peptide binding prediction. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-296"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/9780470122778.ch6","article-title":"Solid-Phase Peptide Synthesis","volume":"Volume 32","author":"Merrifield","year":"1969","journal-title":"Advances in Enzymology and Related Areas of Molecular Biology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5131","DOI":"10.1073\/pnas.82.15.5131","article-title":"General method for the rapid solid-phase synthesis of large numbers of peptides: Specificity of antigen-antibody interaction at the level of individual amino acids","volume":"82","author":"Houghten","year":"1985","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.2147\/IJN.S29506","article-title":"PLGA nanoparticle-mediated delivery of tumor antigenic peptides elicits effective immune responses","volume":"7","author":"Ma","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetpar.2004.07.023","article-title":"Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant\/immunomodulator system ADAD","volume":"126","author":"Ramajo","year":"2004","journal-title":"Vet. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6091","DOI":"10.1016\/j.bmcl.2006.08.113","article-title":"Synthesis and evaluation of some lipidic aminoalcohols and diamines as immunomodulators","volume":"16","author":"Plaza","year":"2006","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"303","DOI":"10.4103\/0976-500X.119726","article-title":"How to calculate sample size in animal studies?","volume":"4","author":"Charan","year":"2013","journal-title":"J. Pharmacol. Pharmacother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1093\/ilar.43.4.244","article-title":"Guidelines for the design and statistical analysis of experiments using laboratory animals","volume":"43","author":"Festing","year":"2002","journal-title":"ILAR J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/s13071-016-1500-y","article-title":"Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems","volume":"9","author":"Vicente","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1007\/s00436-012-3151-z","article-title":"Susceptibility or resistance of praziquantel in human schistosomiasis: A review","volume":"111","author":"Wang","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.3109\/13880209.2014.970289","article-title":"Evaluation of a method for induction of praziquantel resistance in Schistosoma mansoni","volume":"53","author":"Lotfy","year":"2015","journal-title":"Pharm. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2988","DOI":"10.1016\/j.vaccine.2016.03.079","article-title":"Advancing a vaccine to prevent human schistosomiasis","volume":"34","author":"Merrifield","year":"2016","journal-title":"Vaccine"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"28665","DOI":"10.3402\/jev.v4.28665","article-title":"Protein and small non-coding RNA-enriched extracellular vesicles are released by the pathogenic blood fluke Schistosoma mansoni","volume":"4","author":"Nowacki","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijpara.2015.09.002","article-title":"Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates","volume":"46","author":"Sotillo","year":"2016","journal-title":"Int. J. Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Cai, P., Liu, S., Piao, X., Hou, N., Gobert, G.N., McManus, D.P., and Chen, Q. (2016). Comprehensive Transcriptome Analysis of Sex-Biased Expressed Genes Reveals Discrete Biological and Physiological Features of Male and Female Schistosoma japonicum. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004684"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13071-016-1947-x","article-title":"A next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke Schistosoma japonicum","volume":"10","author":"Cai","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3459","DOI":"10.1021\/cr100223m","article-title":"Structural and immunological principles leading to chemically synthesized, multiantigenic, multistage, minimal subunit-based vaccine development","volume":"111","author":"Patarroyo","year":"2011","journal-title":"Chem. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Agallou, M., Margaroni, M., Athanasiou, E., Toubanaki, D.K., Kontonikola, K., Karidi, K., Kammona, O., Kiparissides, C., and Karagouni, E. (2017). Identification of BALB\/c Immune Markers Correlated with a Partial Protection to Leishmania infantum after Vaccination with a Rationally Designed Multi-epitope Cysteine Protease A Peptide-Based Nanovaccine. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005311"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"609994","DOI":"10.3389\/fimmu.2020.609994","article-title":"Arachidonic Acid Is a Safe and Efficacious Schistosomicide, and an Endoschistosomicide in Natural and Experimental Infections, and Cysteine Peptidase Vaccinated Hosts","volume":"11","author":"Tallima","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"McManus, D.P. (2020). Recent progress in the development of liver fluke and blood fluke vaccines. Vaccines, 8.","DOI":"10.3390\/vaccines8030553"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e00176","DOI":"10.1016\/j.parepi.2020.e00176","article-title":"Current status and future prospects of protein vaccine candidates against Schistosoma mansoni infection","volume":"11","author":"Eyayu","year":"2020","journal-title":"Parasite Epidemiol. Control"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1006\/expr.1995.1120","article-title":"Schistosoma mansoni: Excretory-secretory polypeptides exhibit selective binding to plasma components of the snail Biomphalaria glabrata","volume":"81","author":"Davids","year":"1995","journal-title":"Exp. Parasitol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1007\/s00436-012-3092-6","article-title":"Molecular cloning and characterization of glutamine synthetase, a tegumental protein from Schistosoma japonicum","volume":"111","author":"Qiu","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1007\/s00709-020-01515-y","article-title":"Pathogen-host interaction mediated by vesicle-based secretion in schistosomes","volume":"257","author":"Bischofsberger","year":"2020","journal-title":"Protoplasma"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ijid.2017.10.024","article-title":"Kunitz-type protease inhibitor as a vaccine candidate against schistosomiasis mansoni","volume":"66","author":"Ranasinghe","year":"2018","journal-title":"Int. J. Infect. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.3389\/fimmu.2018.01762","article-title":"Schistosoma mansoni SmKI-1 or Its C-Terminal Fragment Induces Partial Protection Against S. mansoni Infection in Mice","volume":"9","author":"Morais","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.3389\/fimmu.2019.02498","article-title":"Peptides Derived of Kunitz-Type Serine Protease Inhibitor as Potential Vaccine Against Experimental Schistosomiasis","volume":"10","author":"Protasio","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Hassan, A.S., Zelt, N.H., Perera, D.J., Ndao, M., and Ward, B.J. (2019). Vaccination against the digestive enzyme Cathepsin B using a YS1646 Salmonella enterica Typhimurium vector provides almost complete protection against Schistosoma mansoni challenge in a mouse model. PLoS Negl. Trop. Dis., 13.","DOI":"10.1101\/652644"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Tallima, H., Dvo\u0159\u00e1k, J., Kareem, S., Abou El Dahab, M., Abdel Aziz, N., Dalton, J.P., and El Ridi, R. (2017). Protective immune responses against Schistosoma mansoni infection by immunization with functionally active gut-derived cysteine peptidases alone and in combination with glyceraldehyde 3-phosphate dehydrogenase. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005443"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.bmc.2015.01.033","article-title":"The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni","volume":"23","author":"Roubille","year":"2015","journal-title":"Bioorganic Med. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1186\/s13071-017-2227-0","article-title":"Developing a mathematical model for the evaluation of the potential impact of a partially efficacious vaccine on the transmission dynamics of Schistosoma mansoni in human communities","volume":"10","author":"Stylianou","year":"2017","journal-title":"Parasites Vectors"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/10\/3\/445\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:14:40Z","timestamp":1760159680000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/10\/3\/445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,24]]},"references-count":55,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["jcm10030445"],"URL":"https:\/\/doi.org\/10.3390\/jcm10030445","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,24]]}}}