{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:30:59Z","timestamp":1777555859977,"version":"3.51.4"},"reference-count":26,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016643","name":"Japan Advanced Institute of Science and Technology","doi-asserted-by":"publisher","award":["JP239149"],"award-info":[{"award-number":["JP239149"]}],"id":[{"id":"10.13039\/100016643","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>In the current clinical islet transplantation, intraportal transplantation is regarded as the gold-standard procedure. However, in this procedure, 50 to 70% of the transplanted islets are immediately damaged due to a strong innate immune response based on islet\u2013blood contact. We investigated the transplant efficiency of a novel method of liver surface transplantation using a syngeneic keratinocyte sheet to avoid islet\u2013blood contact. To examine the influence of the keratinocyte sheet, substantial amounts of syngeneic islets (8 IEQs\/g) were transplanted on the liver surface of diabetic rats, while marginal amounts of islets (4 IEQs\/g) were transplanted via intraportal transplantation to compare the transplant efficiency. Blood glucose, intraperitoneal glucose tolerance, immunohistochemistry, and in vivo imaging findings of the cell sheet were evaluated. The study showed that islet transplantation to the liver surface immediately followed by a syngeneic keratinocyte sheet covering was effective for curing diabetic rats, while no rats were cured in the group without the cell sheet. Notably, islet grafts transplanted via this approach appeared to penetrate into the liver parenchyma. However, the transplant efficiency did not reach that of intraportal transplantation. Further refinements of this approach by introducing mesothelial or fibroblast cell sheets in combination with a preferable scaffold for islet grafts may help to improve the transplant efficiency.<\/jats:p>","DOI":"10.3390\/jcm10040724","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T18:45:00Z","timestamp":1613155500000},"page":"724","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Liver Surface Is an Attractive Transplant Site for Pancreatic Islet Transplantation"],"prefix":"10.3390","volume":"10","author":[{"given":"Akiko","family":"Inagaki","sequence":"first","affiliation":[{"name":"Division of Transplantation and Regenerative Medicine, Graduate School of Medicine, Tohoku University, Sendai 980-0872, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiro","family":"Imura","sequence":"additional","affiliation":[{"name":"Division of Transplantation and Regenerative Medicine, Graduate School of Medicine, Tohoku University, Sendai 980-0872, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Pathology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai 983-8536, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Ohashi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Goto","sequence":"additional","affiliation":[{"name":"Division of Transplantation and Regenerative Medicine, Graduate School of Medicine, Tohoku University, Sendai 980-0872, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1056\/NEJM200007273430401","article-title":"Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen","volume":"343","author":"Shapiro","year":"2000","journal-title":"N. 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