{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:09:26Z","timestamp":1790507366678,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T00:00:00Z","timestamp":1614902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Traditionally, the diagnostic mainstay of recurrent urinary tract infection has been urinary culture. However, the causative uropathogen of recurrent cystitis has not been well established. Urine DNA next-generation sequencing (NGS) can provide additional information on these infections. Herein, we compared urine NGS results and urine cultures in patients with acute uncomplicated cystitis (AUC) and recurrent cystitis (RC), and evaluated the difference in microbiome patterns in the NGS results. Patients who underwent urine culture and NGS due to AUC or RC were retrospectively reviewed. All urine samples were collected via a transurethral catheter and studied utilizing a type of NGS called 16S ribosomal RNA gene amplification and sequencing. The sensitivity of urine NGS was significantly higher than that of conventional urine culture (69.0% vs. 16.7%, p &lt; 0.05). The detection rate of urine NGS was slightly lower in the RC group than in the AUC group (67.7% vs. 72.7%). Microbiome diversity was significantly higher in the RC group compared to the AUC group (p = 0.007), and the microbiome composition was significantly different between the AUC and RC groups. In the urine NGS results, Pseudomonas, Acinetobacter, and Enterobacteriaceae were found in the AUC group, and Sphingomonas, Staphylococcus, Streptococcus, and Rothia spp. were detected in the RC group. Urine NGS can significantly increase the diagnostic sensitivity compared to traditional urine culture methods, especially in RC patients. AUC and RC patients had significant differences in bacterial diversity and patterns. Therefore, recurrent cystitis might be approached from a different perspective.<\/jats:p>","DOI":"10.3390\/jcm10051097","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T11:46:09Z","timestamp":1614944769000},"page":"1097","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7802-0381","authenticated-orcid":false,"given":"Jeong-Ju","family":"Yoo","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5602-5723","authenticated-orcid":false,"given":"Hee","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Song","sequence":"additional","affiliation":[{"name":"GC Genome, Green Cross Laboratories, Department of Laboratory Medicine, Yongin 16924, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjung","family":"Kim","sequence":"additional","affiliation":[{"name":"GC Genome, Green Cross Laboratories, Department of Laboratory Medicine, Yongin 16924, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5897-8309","authenticated-orcid":false,"given":"Jina","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1413-2800","authenticated-orcid":false,"given":"Zisun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Surgery, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Woo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-Yeon","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 31151, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3959-0928","authenticated-orcid":false,"given":"Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Urology, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"on the behalf of SMS (Soonchunhyang microbiome multi-disciplinary study group)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1086\/318850","article-title":"Urinary tract infections: Disease panorama and challenges","volume":"183","author":"Stamm","year":"2001","journal-title":"J. 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