{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:26:56Z","timestamp":1792873616198,"version":"4.5.0"},"reference-count":22,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T00:00:00Z","timestamp":1616371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012774","name":"Innovation Fund Denmark","doi-asserted-by":"publisher","award":["5016-000088"],"award-info":[{"award-number":["5016-000088"]}],"id":[{"id":"10.13039\/100012774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004196","name":"Odense University Hospital","doi-asserted-by":"publisher","award":["1620 (A-nummer)"],"award-info":[{"award-number":["1620 (A-nummer)"]}],"id":[{"id":"10.13039\/501100004196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Sperm DNA fragmentation index (DFI) can be analyzed by a flow cytometric assay after treatment with acid and acridine orange. In this prospective, cohort study, the value of DFI was determined in a semen analysis collected before fertility treatment (baselineDFI) in 146 couples and during 1\u20133 intrauterine inseminations (IUI) in 211 couples (511 cycles). The pregnancy rate (PR)\/cycle was 9.9% if baselineDFI was &gt;10 and 21.7% if baselineDFI was \u226410, (p &lt; 0.005). The live birth rate (LBR)\/cycle was 5% if baselineDFI was &gt;10 and 14.2% if baselineDFI was \u226410 (p &lt; 0.005). PR\/patient was 23.1% if baselineDFI was &gt;10 and 45.5% if baselineDFI was \u226410 (p &lt; 0.005). LBR\/patient was 12.4% if baselineDFI was &gt;10 and 34% if baselineDFI was \u226410 (p &lt; 0.005). When isolating non-stimulated IUI cycles and couples with female age &lt; 35, a significant difference in PR and LBR between couples with high DFI and low DFI was seen. Results suggest that DFI &gt; 10 could advice against timed coitus and non-stimulated IUI cycles. Analysis for DFI performed before treatment provides information about PR and LBR after IUI.<\/jats:p>","DOI":"10.3390\/jcm10061310","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T12:55:07Z","timestamp":1616417707000},"page":"1310","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["DNA Fragmentation in Human Spermatozoa and Pregnancy Rates after Intrauterine Insemination. Should the DFI Threshold Be Lowered?"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7756-3862","authenticated-orcid":false,"given":"Anne Sofie","family":"Rex","sequence":"first","affiliation":[{"name":"Centre of Andrology &amp; Fertility Clinic, Odense University Hospital, 5000 Odense, Denmark"},{"name":"Aagaard Gynecological Clinic, Skejby, 8200 Aarhus, Denmark"},{"name":"Research Unit of Gynecology and Obstetrics, Faculty of Health, University of Southern Denmark, 5000 Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsen","family":"Wu","sequence":"additional","affiliation":[{"name":"Centre of Andrology &amp; Fertility Clinic, Odense University Hospital, 5000 Odense, Denmark"},{"name":"Research Unit of Gynecology and Obstetrics, Faculty of Health, University of Southern Denmark, 5000 Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rn","family":"Aagaard","sequence":"additional","affiliation":[{"name":"Aagaard Gynecological Clinic, Skejby, 8200 Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5383-5501","authenticated-orcid":false,"given":"Jens","family":"Fedder","sequence":"additional","affiliation":[{"name":"Centre of Andrology &amp; Fertility Clinic, Odense University Hospital, 5000 Odense, Denmark"},{"name":"Research Unit of Gynecology and Obstetrics, Faculty of Health, University of Southern Denmark, 5000 Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s10815-015-0624-x","article-title":"Impact of sperm DNA chromatin in the clinic","volume":"33","author":"Ioannou","year":"2016","journal-title":"J. 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