{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T00:02:36Z","timestamp":1781308956425,"version":"3.54.1"},"reference-count":31,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:00:00Z","timestamp":1619395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FRENCH MINISTRY OF HEALTH","award":["PHRC 2010 (10-052)"],"award-info":[{"award-number":["PHRC 2010 (10-052)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Dietary supplementation is commonly used in men with male infertility but its exact role is poorly understood. The aim of this multicenter, randomized, double-blind, placebo-controlled trial was to evaluate the impact of high-dose folic acid supplementation on IVF-ICSI outcomes. 162 couples with male infertility and an indication for IVF-ICSI were included for one IVF-ICSI cycle. Male partners of couples wishing to conceive, aged 18\u201360 years old, with at least one abnormal spermatic criterion were randomized in a 1:1 ratio to receive daily supplements containing 15 mg of folic acid or a placebo for 3 months from Day 0 until semen collection for IVF-ICSI. Sperm parameters and DNA fragmentation before and after the treatment and the biochemical and clinical pregnancy rates after the fresh embryo transfer were analyzed. We observed an increase in the biochemical pregnancy rate and a trend for a higher clinical pregnancy rate in the folic acid group compared to placebo (44.1% versus 22.4%, p = 0.01 and 35.6% versus 20.4%, p = 0.082, respectively). Even if no changes in sperm characteristics were observed, a decrease in DNA fragmentation in the folic acid group was noted (8.5 \u00b1 4.5 vs. 6.4 \u00b1 4.6, p &lt; 0.0001). High-dose folic acid supplementation in men requiring IVF-ICSI for male infertility improves IVF-ICSI outcomes.<\/jats:p>","DOI":"10.3390\/jcm10091876","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T02:31:43Z","timestamp":1619490703000},"page":"1876","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["High-Dose Supplementation of Folic Acid in Infertile Men Improves IVF-ICSI Outcomes: A Randomized Controlled Trial (FOLFIV Trial)"],"prefix":"10.3390","volume":"10","author":[{"given":"Emmanuelle","family":"Mathieu d\u2019Argent","sequence":"first","affiliation":[{"name":"Service de Gyn\u00e9cologie Obst\u00e9trique et M\u00e9decine de la Reproduction, AP-HP Sorbonne Universit\u00e9 site Tenon, 4 rue de la Chine, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Ravel","sequence":"additional","affiliation":[{"name":"CHU Rennes, Laboratoire de Biologie de la Reproduction-CECOS, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail)-UMR_S 1085, Universit\u00e9 de Rennes, 35000 Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Clinical Research Platform of East of Paris (URCEST-CRC-CRB), APHP Sorbonne Universite, H\u00f4pital Saint Antoine, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Morcel","sequence":"additional","affiliation":[{"name":"Centre d\u2019Assistance M\u00e9dicale \u00e0 la Procr\u00e9ation, Service de Gyn\u00e9cologie-Obst\u00e9trique, CHU de Brest, H\u00f4pital Morvan, 29609 Brest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Massin","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Intercommunal de Cr\u00e9teil, Department of Gynecology, Obstetrics and Reproductive Medicine, Universit\u00e9 Paris-Est Cr\u00e9teil, 94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Sussfeld","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie Obst\u00e9trique et M\u00e9decine de la Reproduction, AP-HP Sorbonne Universit\u00e9 site Tenon, 4 rue de la Chine, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tabassome","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Clinical Research Platform of East of Paris (URCEST-CRC-CRB), APHP Sorbonne Universite, H\u00f4pital Saint Antoine, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Antoine","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie Obst\u00e9trique et M\u00e9decine de la Reproduction, AP-HP Sorbonne Universit\u00e9 site Tenon, 4 rue de la Chine, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Mandelbaume","sequence":"additional","affiliation":[{"name":"Service de Biologie de la Reproduction, Centre d\u2019Etudes et de Conservation des \u0153ufs et du Sperme Humains (CECOS), H\u00f4pital Tenon, Assistance Publique-H\u00f4pitaux de Paris (APHP), Universit\u00e9 Paris 06, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emile","family":"Dara\u00ef","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie Obst\u00e9trique et M\u00e9decine de la Reproduction, AP-HP Sorbonne Universit\u00e9 site Tenon, 4 rue de la Chine, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7445-5345","authenticated-orcid":false,"given":"Kamila","family":"Kolanska","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie Obst\u00e9trique et M\u00e9decine de la Reproduction, AP-HP Sorbonne Universit\u00e9 site Tenon, 4 rue de la Chine, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/S0015-0282(02)03105-9","article-title":"Best practice policies for male infertility","volume":"77","author":"Sharlip","year":"2002","journal-title":"Fertil. 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