{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:40:11Z","timestamp":1789663211728,"version":"4.0.1"},"reference-count":67,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T00:00:00Z","timestamp":1621555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Glaucoma Association for glaucoma patients and families AGAF","award":["0111 46 1307147 76"],"award-info":[{"award-number":["0111 46 1307147 76"]}]},{"name":"VLC_BIOMED","award":["29\/2016A"],"award-info":[{"award-number":["29\/2016A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Glaucoma has no cure and is a sight-threatening neurodegenerative disease affecting more than 100 million people worldwide, with primary open angle glaucoma (POAG) being the most globally prevalent glaucoma clinical type. Regulation of gene expression and gene networks, and its multifactorial pathways involved in glaucoma disease are landmarks for ophthalmic research. MicroRNAs (miRNAs\/miRs) are small endogenous non-coding, single-stranded RNA molecules (18\u201322 nucleotides) that regulate gene expression. An analytical, observational, case-control study was performed in 42 patients of both sexes, aged 50 to 80 years, which were classified according to: (1) suffering from ocular hypertension (OHT) but no glaucomatous neurodegeneration (ND) such as the OHT group, or (2) have been diagnosed of POAG such as the POAG group. Participants were interviewed for obtaining sociodemographic and personal\/familial records, clinically examined, and their tear samples were collected and frozen at 80 \u00b0C until processing for molecular-genetic assays. Tear RNA extraction, libraries construction, and next generation sequencing were performed. Here, we demonstrated, for the first time, the differential expression profiling of eight miRNAs when comparing tears from the OHT versus the POAG groups: the miR-26b-5p, miR-152-3p, miR-30e-5p, miR-125b-2-5p, miR-224-5p, miR-151a-3p, miR-1307-3p, and the miR-27a-3p. Gene information was set up from the DIANA-TarBase v7, DIANA-microT-CDS, and TargetScan v7.1 databases. To build a network of metabolic pathways, only genes appearing in at least four of the following databases: DisGeNet, GeneDistiller, MalaCards, OMIM PCAN, UniProt, and GO were considered. We propose miRNAs and their target genes\/signaling pathways as candidates for a better understanding of the molecular-genetic bases of glaucoma and, in this way, to gain knowledge to achieve optimal diagnosis strategies for properly identifying HTO at higher risk of glaucoma ND. Further research is needed to validate these miRNAs to discern the potential role as biomarkers involved in oxidative stress, immune response, and apoptosis for the diagnosis and\/or prognosis of OHT and the prevention of glaucoma ND.<\/jats:p>","DOI":"10.3390\/jcm10112227","type":"journal-article","created":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T13:15:15Z","timestamp":1621602915000},"page":"2227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["miRNAs and Genes Involved in the Interplay between Ocular Hypertension and Primary Open-Angle Glaucoma. Oxidative Stress, Inflammation, and Apoptosis Networks"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-0798","authenticated-orcid":false,"given":"Jorge","family":"Raga-Cervera","sequence":"first","affiliation":[{"name":"Hospital of Manises, 46940 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose M.","family":"Bolarin","sequence":"additional","affiliation":[{"name":"Technological Centre of Information and Communication Technologies (CENTIC), 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7211-9129","authenticated-orcid":false,"given":"Jose M.","family":"Millan","sequence":"additional","affiliation":[{"name":"Sequencing Service at the University and Polytechnic Hospital La Fe, 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6245-7271","authenticated-orcid":false,"given":"Jose J.","family":"Garcia-Medina","sequence":"additional","affiliation":[{"name":"Ophthalmic Research Unit \u201cSantiago Grisol\u00eda\u201d\/FISABIO, 46017 Valencia, Spain"},{"name":"Department of Ophthalmology, General University Hospital \u201cMorales Meseguer\u201d, 30007 Murcia, Spain"},{"name":"Department of Ophthalmology and Optometry, University of Murcia, 30120 Murcia, Spain"},{"name":"Spanish Net of Ophthalmic Research OFTARED RD16\/0008\/0022, Institute of Health Carlos III., 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Pedrola","sequence":"additional","affiliation":[{"name":"Sequencing Service at the University and Polytechnic Hospital La Fe, 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Abell\u00e1n-Abenza","sequence":"additional","affiliation":[{"name":"Technological Centre of Information and Communication Technologies (CENTIC), 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6466-9377","authenticated-orcid":false,"given":"Mar","family":"Valero-Vello","sequence":"additional","affiliation":[{"name":"Ophthalmic Research Unit \u201cSantiago Grisol\u00eda\u201d\/FISABIO, 46017 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9569-1451","authenticated-orcid":false,"given":"Silvia M.","family":"Sanz-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Ophthalmic Research Unit \u201cSantiago Grisol\u00eda\u201d\/FISABIO, 46017 Valencia, Spain"},{"name":"Spanish Net of Ophthalmic Research OFTARED RD16\/0008\/0022, Institute of Health Carlos III., 28029 Madrid, Spain"},{"name":"Cellular and Molecular Ophthalmobiology Group, Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E.","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Laboratory of Cytomics, Joint Research Unit Principe Felipe Research Center and University of Valencia, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Galarreta-Mira","sequence":"additional","affiliation":[{"name":"University Clinic Hospital of Valladolid, 47003 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Bendala-Tufanisco","sequence":"additional","affiliation":[{"name":"Spanish Net of Ophthalmic Research OFTARED RD16\/0008\/0022, Institute of Health Carlos III., 28029 Madrid, Spain"},{"name":"Mixed Research Unit for Visual Health and Veterinary Ophthalmology CEU\/FISABIO, 46020 Valencia, Spain"},{"name":"Physiology Department, Faculty of Health Sciences, CEU University, Alfara del Patriarca, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aloma","family":"Mayordomo-Febrer","sequence":"additional","affiliation":[{"name":"Spanish Net of Ophthalmic Research OFTARED RD16\/0008\/0022, Institute of Health Carlos III., 28029 Madrid, Spain"},{"name":"Mixed Research Unit for Visual Health and Veterinary Ophthalmology CEU\/FISABIO, 46020 Valencia, Spain"},{"name":"Animal Medicine and Surgery Department, Veterinary Medicine Faculty, CEU University, Alfara del Patriarca, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-7837","authenticated-orcid":false,"given":"Maria D.","family":"Pinazo-Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Ophthalmic Research Unit \u201cSantiago Grisol\u00eda\u201d\/FISABIO, 46017 Valencia, Spain"},{"name":"Spanish Net of Ophthalmic Research OFTARED RD16\/0008\/0022, Institute of Health Carlos III., 28029 Madrid, Spain"},{"name":"Cellular and Molecular Ophthalmobiology Group, Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1179-1592","authenticated-orcid":false,"given":"Vicente","family":"Zan\u00f3n-Moreno","sequence":"additional","affiliation":[{"name":"Ophthalmic Research Unit \u201cSantiago Grisol\u00eda\u201d\/FISABIO, 46017 Valencia, Spain"},{"name":"Spanish Net of Ophthalmic Research OFTARED RD16\/0008\/0022, Institute of Health Carlos III., 28029 Madrid, Spain"},{"name":"Faculty of Health Sciences, Valencian International University, 46002 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ophtha.2015.10.053","article-title":"Primary Open-Angle Glaucoma Preferred Practice Pattern(\u00ae) Guidelines","volume":"123","author":"Prum","year":"2016","journal-title":"Ophthalmology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1016\/j.ophtha.2014.05.013","article-title":"Global prevalence of glaucoma and projections of glaucoma burden through 2040: A systematic review and meta-analysis","volume":"121","author":"Tham","year":"2014","journal-title":"Ophthalmology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S162","DOI":"10.1016\/j.survophthal.2007.08.012","article-title":"What is the present pathogenetic concept of glaucomatous optic neuropathy?","volume":"52","author":"Flammer","year":"2007","journal-title":"Surv. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1442-9071.2010.02462.x","article-title":"Glaucomatous optic neuropathy evaluation project: A standardized internet system for assessing skills in optic disc examination","volume":"39","author":"Kong","year":"2010","journal-title":"Clin. Exp. Ophthalmol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ajo.2017.09.020","article-title":"Comparison of glaucoma progression detection by optical coherence tomography and visual field","volume":"184","author":"Zhang","year":"2017","journal-title":"Am. J. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Parra-Blesa, A., Sanchez-Alberca, A., and Garcia-Medina, J.J. (2020). Clinical-evolutionary staging system of primary open-angle glaucoma using optical coherence tomography. J. Clin. Med., 9.","DOI":"10.3390\/jcm9051530"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1001\/archopht.1977.04450040054004","article-title":"\u2018Glaucoma Suspect\u2019 or \u2018Ocular Hypertension\u2019?","volume":"95","author":"Shaffer","year":"1977","journal-title":"Arch. Ophthalmol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"33373","DOI":"10.1038\/srep33373","article-title":"Quadrant field pupillometry detects melanopsin dysfunction in glaucoma suspects and early glaucoma","volume":"6","author":"Adhikari","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4103\/1673-5374.286953","article-title":"Roles of the ocular pressure, pressure-sensitive ion channel, and elasticity in pressure-induced retinal diseases","volume":"16","author":"Pang","year":"2021","journal-title":"Neural Regen. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1001\/archopht.120.10.1268","article-title":"Reduction of intraocular pressure and glaucoma progression: Results from the Early Manifest Glaucoma Trial","volume":"120","author":"Heijl","year":"2002","journal-title":"Arch. Ophthalmol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lo, K.-J., Ko, Y.-C., Hwang, D.-K., and Liu, C.J.-L. (2020). The influence of topical non-steroidal anti-inflammatory drugs on the intraocular pressure lowering effect of topical prostaglandin analogues\u2014A systemic review and meta-analysis. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0239233"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"903","DOI":"10.2174\/1570159X15666170705101910","article-title":"Strategies to reduce oxidative stress in glaucoma patients","volume":"16","year":"2018","journal-title":"Curr. Neuropharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"197","DOI":"10.4103\/1673-5374.244781","article-title":"Evidence on neuroprotective properties of coenzyme Q10 in the treatment of glaucoma","volume":"14","author":"Nucci","year":"2019","journal-title":"Neural Regen. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.4103\/1673-5374.274325","article-title":"Beneficial effects of saffron (Crocus sativus L.) in ocular pathologies, particularly neurodegenerative retinal diseases","volume":"15","author":"Salazar","year":"2020","journal-title":"Neural Regen. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Pinazo-Dur\u00e1n, M.D., Garc\u00eda-Medina, J.J., Bolar\u00edn, J.M., Sanz-Gonz\u00e1lez, S.M., Valero-Vello, M., Abell\u00e1n-Abenza, J., Zan\u00f3n-Moreno, V., and Moreno-Monta\u00f1\u00e9s, J. (2020). Computational analysis of clinical and molecular markers and new theranostic possibilities in primary open-angle glaucoma. J. Clin. Med., 9.","DOI":"10.3390\/jcm9093032"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most mammalian mRNAs are con-served targets of microRNAs","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6033","DOI":"10.1038\/s41467-020-19865-9","article-title":"Quantification of purified endogenous miRNAs with high sensitivity and spec-ificity","volume":"11","author":"Shin","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_18","first-page":"1136","article-title":"[Expression and function of microRNA in the eye]","volume":"48","author":"Zhang","year":"2012","journal-title":"[Zhonghua yan ke za zhi] Chin. J. Ophthalmol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/s41598-017-00788-3","article-title":"Multimodal regulation orchestrates normal and complex disease states in the retina","volume":"7","author":"Olivares","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.exer.2013.03.009","article-title":"FOXC1 in human trabecu-lar meshwork cells is involved in regulatory pathway that includes miR-204, MEIS2, and ITG\u03b21","volume":"111","author":"Paylakhi","year":"2013","journal-title":"Exp. Eye. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1038\/s41598-017-01003-z","article-title":"Regulation of intraocular pressure by microRNA cluster miR-143\/145","volume":"7","author":"Li","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5089","DOI":"10.1038\/srep05089","article-title":"Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system","volume":"4","author":"Tanaka","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1167\/iovs.18-24878","article-title":"Identification of candidate miRNA Biomarkers for Glaucoma","volume":"60","author":"Hindle","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.cellsig.2018.11.006","article-title":"Neuroprotective effects of overexpressed microRNA-200a on acti-vation of glaucoma-related retinal glial cells and aoptosis of ganglion cells via downregulating FGF7-mediated MAPK signaling pathway","volume":"54","author":"Peng","year":"2019","journal-title":"Cell Signal."},{"key":"ref_25","first-page":"437","article-title":"Cytokine expression in tears of patients with glaucoma or dry eye disease: A prospective, observational cohort study","volume":"29","year":"2018","journal-title":"Eur. J. Ophthalmol."},{"key":"ref_26","first-page":"297","article-title":"Tear 1H NMR-based metabolomics application to the molecular diagnosis of aqueous tear deficiency and Mei-bomian gland dysfunction","volume":"64","year":"2020","journal-title":"Ophthalmic Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.ajo.2005.07.044","article-title":"Categorizing the stage of glaucoma from pre-diagnosis to end-stage disease","volume":"141","author":"Mills","year":"2006","journal-title":"Am. J. Ophthalmol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1186\/s12859-019-2974-3","article-title":"miRFA: An automated pipeline for microRNA functional analysis with correlation support from TCGA and TCPA expression data in pancreatic cancer","volume":"20","author":"Sund","year":"2019","journal-title":"BMC Bioinform."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3637","DOI":"10.1093\/hmg\/ddz222","article-title":"Identification of genes involved in glaucoma pathogenesis using combined network analysis and empirical studies","volume":"28","author":"Moazzeni","year":"2019","journal-title":"Hum. Mol. Genet."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"D833","DOI":"10.1093\/nar\/gkw943","article-title":"DisGeNET: A comprehensive platform integrating information on human disease-associated genes and variants","volume":"45","author":"Bravo","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/s41596-018-0103-9","article-title":"Pathway enrichment analysis and visualization of omics data using g:Profiler, GSEA, Cytoscape and EnrichmentMap","volume":"14","author":"Reimand","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"W60","DOI":"10.1093\/nar\/gky311","article-title":"GeneMANIA update","volume":"46","author":"Franz","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1007\/s10067-019-04728-6","article-title":"Altered expression of circular RNA in primary Sj\u00f6gren\u2019s syndrome","volume":"38","author":"Su","year":"2019","journal-title":"Clin. Rheumatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s00592-019-01448-w","article-title":"Comparison of expression profiling of circular RNAs in vitreous humour between diabetic retinopathy and non-diabetes mellitus patients","volume":"57","author":"He","year":"2020","journal-title":"Acta Diabetol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1080\/00365513.2019.1637931","article-title":"Three new circulating microRNAs may be associated with wet age-related macular degeneration","volume":"79","author":"Elbay","year":"2019","journal-title":"Scand. J. Clin. Lab. Investig."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1167\/iovs.61.5.2","article-title":"Expression of mRNAs, miRNAs, and lncRNAs in human trabecular meshwork cells upon mechanical stretch","volume":"61","author":"Youngblood","year":"2020","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2955","DOI":"10.1167\/iovs.17-23599","article-title":"microRNA profiling in glaucoma eyes with varying degrees of optic neuropathy by using next-generation sequencing","volume":"59","author":"Liu","year":"2018","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_38","first-page":"1057","article-title":"Evaluation of circulating miRNAs in wet age-related macular degeneration","volume":"20","author":"Ertekin","year":"2014","journal-title":"Mol. Vis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1097\/IJG.0b013e31815c3a7f","article-title":"Oxidative stress in primary open-angle glaucoma","volume":"17","year":"2008","journal-title":"J. Glaucoma"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4078","DOI":"10.1167\/iovs.17-22242","article-title":"Oxidative stress-related molecular biomarker candidates for glaucoma","volume":"58","author":"Hondur","year":"2017","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.exer.2012.07.011","article-title":"Aqueous humor oxidative stress proteomic levels in primary open angle glaucoma","volume":"103","author":"Bagnis","year":"2012","journal-title":"Exp. Eye Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.2147\/OPTH.S254504","article-title":"Differential miRNA Expression: Signature for glaucoma in pseudoexfoliation","volume":"14","author":"Rao","year":"2020","journal-title":"Clin. Ophthalmol."},{"key":"ref_43","first-page":"2825","article-title":"miR-27a-3p regulates proliferation and apoptosis of colon cancer cells by potentially targeting BTG1","volume":"18","author":"Su","year":"2019","journal-title":"Oncol. Lett."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.2147\/CMAR.S234897","article-title":"MiR-27a-3p Regulated the aggressive phenotypes of cervical cancer by targeting FBXW7","volume":"12","author":"Ben","year":"2020","journal-title":"Cancer Manag. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"104718","DOI":"10.1016\/j.phrs.2020.104718","article-title":"MicroRNA-27a-3p aggravates renal ischemia\/reperfusion injury by promoting oxidative stress via targeting growth factor receptor-bound protein 2","volume":"155","author":"Zhao","year":"2020","journal-title":"Pharmacol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1089\/jop.2013.0191","article-title":"Role of MicroRNAs in the Trabecular Meshwork","volume":"30","author":"Gonzalez","year":"2014","journal-title":"J. Ocul. Pharmacol. Ther."},{"key":"ref_47","unstructured":"Drewry, M.D., Cai, J., Helwa, I., Hu, E., Liu, S., Mu, H., Hu, Y., Johnson, W.M., Gonzalez, P., and Stamer, W.D. (2020). Genome-wide Expression Profiling and Pathway Analysis in Cyclic Stretched Human Trabecular Meshwork. BioRXiv."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"DesJarlais, M., Rivera, J.C., Lahaie, I., Cagnone, G., Wirt, M., Omri, S., and Chemtob, S. (2019). MicroRNA expression profile in retina and choroid in oxygen-induced retinopathy model. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0218282"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"116779","DOI":"10.1016\/j.lfs.2019.116779","article-title":"MiR-152-3p regulates cell proliferation, invasion and extracellular matrix expression through by targeting FOXF1 in keloid fibroblasts","volume":"234","author":"Wang","year":"2019","journal-title":"Life Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/acn3.692","article-title":"miR-30e-5p as predictor of generalization in ocular myasthenia gravis","volume":"6","author":"Sabre","year":"2019","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_51","first-page":"20131057","article-title":"Constructing and characterizing a bioactive small molecule and microRNA association network for Alzheimer\u2019s disease","volume":"18","author":"Meng","year":"2013","journal-title":"J. R. Soc. Interface"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"640389","DOI":"10.1100\/2012\/640389","article-title":"Neurobiology, Pathophysiology, and treatment of melatonin deficiency and dysfunction","volume":"2012","author":"Hardeland","year":"2012","journal-title":"Sci. World J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4185","DOI":"10.2174\/1381612825666191113110225","article-title":"Melatonin: Implications for ocular disease and therapeutic potential","volume":"25","author":"Scuderi","year":"2019","journal-title":"Curr. Pharm. Des."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1167\/tvst.9.6.23","article-title":"MicroRNAs in the vitreous humor of patients with retinal detachment and a different grading of proliferative vitreoretinopathy: A pilot study","volume":"9","author":"Toro","year":"2020","journal-title":"Transl. Vis. Sci. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Smit, K.N., Chang, J., Derks, K., Vaarwater, J., Brands, T., Verdijk, R.M., Wiemer, E.A., Mensink, H.W., Pothof, J., and De Klein, A. (2019). Aberrant MicroRNA expression and its implications for uveal melanoma metastasis. Cancers, 11.","DOI":"10.3390\/cancers11060815"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"27","DOI":"10.4321\/S0365-66912005000100006","article-title":"Ocular hypertension as the principal indicator of onset of uveal melanoma","volume":"80","author":"Bianciotto","year":"2005","journal-title":"Arch. Soc. Esp. Oftalmol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.exer.2010.07.009","article-title":"The immune response in glaucoma: A perspective on the roles of oxidative stress","volume":"93","author":"Tezel","year":"2011","journal-title":"Exp. Eye Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5816","DOI":"10.1167\/iovs.15-17294","article-title":"Proteomics analysis of molecular risk factors in the ocular hypertensive human retina","volume":"56","author":"Yang","year":"2015","journal-title":"Investig. Opthalmol. Vis. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1703810","DOI":"10.1155\/2017\/1703810","article-title":"Involvement of Nrf2 in ocular diseases","volume":"2017","author":"Batliwala","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1080\/15384101.2019.1593645","article-title":"Down-regulated LAMA4 inhibits oxidative stress-induced apoptosis of retinal ganglion cells through the MAPK signaling pathway in rats with glaucoma","volume":"18","author":"Wang","year":"2019","journal-title":"Cell Cycle"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Vernazza, S., Tirendi, S., Bassi, A.M., Traverso, C.E., and Sacc\u00e0, S.C. (2020). Neuroinflammation in primary open-angle glaucoma. J. Clin. Med., 9.","DOI":"10.3390\/jcm9103172"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Tabak, S., Schreiber-Avissar, S., and Beit-Yannai, E. (2021). Crosstalk between MicroRNA and oxidative stress in primary open-angle glaucoma. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052421"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Velkovska, M.A., Gori\u010dar, K., Blagus, T., Dol\u017ean, V., and Cvenkel, B. (2021). Association of genetic polymorphisms in oxidative stress and inflammation pathways with glaucoma risk and phenotype. J. Clin. Med., 10.","DOI":"10.3390\/jcm10051148"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Zuo, L., Prather, E.R., Stetskiv, M., Garrison, D.E., Meade, J.R., Peace, T.I., and Zhou, T. (2019). Inflammaging and oxidative stress in human diseases: From molecular mechanisms to novel treatments. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20184472"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/S0896-1549(03)00066-X","article-title":"Predictive DNA testing for glaucoma: Reality in 2003","volume":"16","author":"Mackey","year":"2003","journal-title":"Ophthalmol. Clin. N. Am."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.clinbiochem.2005.11.010","article-title":"Molecular diagnostics of genetic eye diseases","volume":"39","author":"Fan","year":"2006","journal-title":"Clin. Biochem."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1038\/s41588-019-0556-y","article-title":"Multitrait analysis of glaucoma identifies new risk loci and enables polygenic prediction of disease susceptibility and progression","volume":"52","author":"Craig","year":"2020","journal-title":"Nat. Genet."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/10\/11\/2227\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:05:15Z","timestamp":1760162715000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/10\/11\/2227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,21]]},"references-count":67,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["jcm10112227"],"URL":"https:\/\/doi.org\/10.3390\/jcm10112227","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,21]]}}}