{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:09:39Z","timestamp":1789049379474,"version":"build-2803163510"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9liuksen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005744","name":"Lastentautien Tutkimuss\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/501100005744","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010135","name":"Finska L\u00e4kares\u00e4llskapet","doi-asserted-by":"publisher","award":["-"],"award-info":[{"award-number":["-"]}],"id":[{"id":"10.13039\/100010135","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Interleukin (IL)-8 (CXCL8), a chemokine involved in neutrophil recruitment, has been implicated in ductular reaction and liver fibrogenesis. We studied liver and serum IL-8 expression in a large biliary atresia (BA) cohort and explored its prognostic and pathophysiological potential. IL-8 expression was assessed in liver utilizing quantitative polymerase chain reaction (qPCR), immunohistochemistry and in situ hybridization and in serum using an enzyme-linked immunosorbent assay, among 115 BA patients, 10 disease controls and 68 normal controls. Results were correlated to portoenterostomy (PE) outcomes, biochemical and histological liver injury, transcriptional markers of fibrosis and cholangiocytes, and expression of other related cytokines. IL-8 was markedly overexpressed in liver and serum of BA patients at PE (n = 88) and in serum samples obtained during postoperative follow-up (n = 40). IL-8 expression in the liver was predominantly in cholangiocytes within areas of ductular reaction. Liver IL-8 mRNA expression correlated positively with its serum concentration, bile ductular proliferation, Metavir fibrosis stage, and transcriptional markers of activated myofibroblasts (ACTA2) and cholangiocytes (KRT19). Taken together, IL-8 may mediate liver injury in BA by promoting ductular reaction and associated liver fibrogenesis. Prognostic value of serum IL-8 to predict native liver survival was limited and confined to the postoperative period after PE.<\/jats:p>","DOI":"10.3390\/jcm10122705","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T11:19:20Z","timestamp":1624015160000},"page":"2705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Prognostic and Pathophysiologic Significance of IL-8 (CXCL8) in Biliary Atresia"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1606-3028","authenticated-orcid":false,"given":"Nimish","family":"Godbole","sequence":"first","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"},{"name":"Section of Pediatric Surgery, Pediatric Liver and Gut Research Group and Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iiris","family":"Nyholm","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"},{"name":"Section of Pediatric Surgery, Pediatric Liver and Gut Research Group and Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hukkinen","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"},{"name":"Section of Pediatric Surgery, Pediatric Liver and Gut Research Group and Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph R.","family":"Davidson","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, GOS-UCL Institute of Child Health, London WC1N 1EH, UK"},{"name":"Department of Pediatric Surgery, King\u2019s College Hospital, London SE5 9RS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanasios","family":"Tyraskis","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, King\u2019s College Hospital, London SE5 9RS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Eloranta","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noora","family":"Andersson","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jouko","family":"Lohi","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e4ivi","family":"Heikkil\u00e4","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5271-2366","authenticated-orcid":false,"given":"Antti","family":"Kyr\u00f6nlahti","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjut","family":"Pihlajoki","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Davenport","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, King\u2019s College Hospital, London SE5 9RS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9452-0661","authenticated-orcid":false,"given":"Markku","family":"Heikinheimo","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"},{"name":"Department of Pediatrics, Washington University in St. Louis, St. Louis, MO 63130, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6016-6334","authenticated-orcid":false,"given":"Mikko P.","family":"Pakarinen","sequence":"additional","affiliation":[{"name":"Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"},{"name":"Section of Pediatric Surgery, Pediatric Liver and Gut Research Group and Pediatric Research Center, Children\u2019s Hospital, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jaut.2016.06.005","article-title":"Biliary atresia: A comprehensive review","volume":"73","author":"Lakshminarayanan","year":"2016","journal-title":"J. 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