{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:52:01Z","timestamp":1788306721538,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T00:00:00Z","timestamp":1631491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Introduction: Obesity, which is a serious problem in children, has a negative impact on many organs, including kidneys, and obesity-related glomerulopathy (ORG) is an increasingly common cause of ESKD (end-stage kidney disease) in adults. Early-detected and -treated glomerular lesions are reversible, so it is important to find a useful marker of early damage. The study aimed to evaluate the albumin-to-creatinine ratio (ACR), urinary alpha-1-acid glycoprotein (\u03b11-AGP), and mRNA of podocyte-specific proteins as indicators of glomerular injury and their relationship with the degree of obesity and metabolic disorders. Materials and Methods: A total of 125 obese children and 33 healthy peers were enrolled. Patients were divided into two groups, depending on SDS BMI values. ACR, \u03b11-AGP, mRNA expression of nephrin, synaptopodin, podocin, and C2AP protein in urine sediment were measured. Results: ACR values did not differ between groups and were within the normal range. \u03b11-AGP and mRNA expression were significantly higher in obese children compared with controls. mRNA expression of the remaining podocyte proteins was similar in both groups. No significant differences concerning all examined parameters were found depending on the degree of obesity. There was a positive significant correlation between \u03b11-AGP and ACR. Conclusions: Increased \u03b11-AGP before the onset of albuminuria suggests its usefulness as a biomarker of early glomerular damage in obese children. An increased podocin mRNA expression also indicates podocyte damage and may be linked to ORG development. The lack of increase in expression of other podocyte proteins suggests that podocin mRNA may be a more specific and sensitive biomarker. The degree of obesity has no impact on the tested parameters, but further studies are needed to confirm it.<\/jats:p>","DOI":"10.3390\/jcm10184129","type":"journal-article","created":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T21:37:12Z","timestamp":1631569032000},"page":"4129","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children"],"prefix":"10.3390","volume":"10","author":[{"given":"Anna","family":"Medy\u0144ska","sequence":"first","affiliation":[{"name":"Department of Pediatric Nephrology, Wroclaw Medical University, 50-367 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Chrzanowska","sequence":"additional","affiliation":[{"name":"Department of Pediatric Endocrinology and Diabetology, Wroclaw Medical University, 50-367 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3216-438X","authenticated-orcid":false,"given":"Katarzyna","family":"Ko\u015bcielska-Kasprzak","sequence":"additional","affiliation":[{"name":"Specialist Laboratory at the Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-367 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Bartoszek","sequence":"additional","affiliation":[{"name":"Specialist Laboratory at the Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-367 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1810-3131","authenticated-orcid":false,"given":"Marcelina","family":"\u017babi\u0144ska","sequence":"additional","affiliation":[{"name":"Specialist Laboratory at the Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-367 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danuta","family":"Zwoli\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Wroclaw Medical University, 50-367 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1093\/ije\/dyz273","article-title":"Has the prevalence of overweight, obesity and central obesity levelled off in the United States? Trends, patterns, disparities, and future projections for the obesity epidemic","volume":"49","author":"Wang","year":"2020","journal-title":"Int. J. Epidemiol."},{"key":"ref_2","unstructured":"World Health Organization (2000). Obesity: Preventing and Managing the Global Epidemic. WHO Expert Consultation, WHO. Available online: http:\/\/whqlibdoc.who.int\/trs\/WHO_TRS_894.pdf?ua=1."},{"key":"ref_3","first-page":"641","article-title":"The prevalence of overweight and obesity among Polish school-aged children and adolescents","volume":"70","author":"Grajda","year":"2017","journal-title":"Prz. Epidemiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1097\/MNH.0000000000000212","article-title":"The dual roles of obesity in chronic kidney disease: A review of the current literature","volume":"25","author":"Rhee","year":"2016","journal-title":"Curr. Opin. Nephrol. Hypertens."},{"key":"ref_5","first-page":"1","article-title":"Which Patients with Obesity Are at Risk for Renal Disease?","volume":"5","author":"Sandino","year":"2021","journal-title":"Nephron"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/978-3-030-10616-4_6","article-title":"The Growing Epidemic of Chronic Kidney Disease: Preventive Strategies to Delay the Risk for Progression to ESRD","volume":"1121","author":"Assadi","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"440","DOI":"10.7326\/0003-4819-81-4-440","article-title":"The nephrotic syndrome: A complication of massive obesity","volume":"81","author":"Weisinger","year":"1974","journal-title":"Ann. Intern. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1007\/s00467-009-1120-2","article-title":"Obesity and preterm birth: Additive risks in the progression of kidney disease in children","volume":"24","author":"Abitbol","year":"2009","journal-title":"Pediatr. Nephrol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1089\/ham.2018.0017","article-title":"Urinary Alpha-1-Acid Glycoprotein Is a Sensitive Marker of Glomerular Protein Leakage at Altitude","volume":"19","author":"Talks","year":"2018","journal-title":"High Alt. Med. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S1359-6101(02)00054-0","article-title":"Alpha(1)-acid glycoprotein: An acute phase protein with inflammatory and immunomodulating properties","volume":"14","author":"Hochepied","year":"2003","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"22174","DOI":"10.1074\/jbc.M109.085464","article-title":"Adipocytokine orosomucoid integrates inflammatory and metabolic signals to preserve energy homeostasis by resolving immoderate inflammation","volume":"285","author":"Lee","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s10059-012-2181-9","article-title":"Orosomucoid serum concentrations and fat depot-specific mRNA and protein expression in humans","volume":"33","author":"Alfadda","year":"2012","journal-title":"Mol. Cells"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1177\/1076029616637185","article-title":"Serum and Urinary Orosomucoid in Young Patients With Type 1 Diabetes: A Link Between Inflammation, Microvascular Complications, and Subclinical Atherosclerosis","volume":"22","author":"Andrawes","year":"2016","journal-title":"Clin. Appl. Thromb. Hemost."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1159\/000013302","article-title":"Urinary excretion of podocytes reflects disease activity in children with glomerulonephritis","volume":"18","author":"Hara","year":"1998","journal-title":"Am. J. Nephrol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1159\/000046097","article-title":"Urinary podocytes in primary focal segmental glomerulosclerosis","volume":"89","author":"Hara","year":"2001","journal-title":"Nephron"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1097\/00000441-200008000-00009","article-title":"Urinary podocytes for the assessment of disease activity in lupus nephritis","volume":"320","author":"Nakamura","year":"2000","journal-title":"Am. J. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1093\/ndt\/15.9.1379","article-title":"Urinary excretion of podocytes in patients with diabetic nephropathy","volume":"15","author":"Nakamura","year":"2000","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1542\/peds.58.2.259","article-title":"Asimple estimate ofglomerular filtration rate inchildren derived from body length and plasma create\u2014Nine","volume":"58","author":"Schwartz","year":"1976","journal-title":"Pediatrics"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1038\/sj.ijo.0803136","article-title":"Microalbuminuria in pediatric obesity: Prevalence and relation to other cardiovascular risk factors","volume":"30","author":"Burgert","year":"2006","journal-title":"Int. J. Obes."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1097\/HJH.0b013e328364bcbf","article-title":"Prevalence and factors related to urinary albumin excretion in obese youths","volume":"31","author":"Lurbe","year":"2013","journal-title":"J. Hypertens."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1590\/2175-8239-jbn-2018-0006","article-title":"Albuminuria and glomerular filtration rate in obese children and adolescents","volume":"41","author":"Sawamura","year":"2019","journal-title":"J. Bras. Nefrol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1080\/00365510802531100","article-title":"Increased urinary orosomucoid excretion: A proposed marker for inflammation and endothelial dysfunction in patients with type 2 diabetes","volume":"69","author":"Christiansen","year":"2009","journal-title":"Scand. J. Clin. Lab. Investig."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.2337\/diacare.27.5.1176","article-title":"Parallel increase in urinary excretion rates of immunoglobulin G, ceruloplasmin, transferrin, and orosomucoid in normoalbuminuric type 2 diabetic patients","volume":"27","author":"Narita","year":"2004","journal-title":"Diabetes Care"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.ekir.2017.01.007","article-title":"The renal pathology of obesity","volume":"2","author":"Tsuboi","year":"2017","journal-title":"Kidney Int. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s13105-015-0389-9","article-title":"Orosomucoid, an acute response protein with multiple modulating activities","volume":"71","author":"Luo","year":"2015","journal-title":"J. Physiol. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1111\/1744-9987.12530","article-title":"Elevated High Sensitivity C-Reactive Protein Increases the Risk of Microalbuminuria in Subjects With Cardiovascular Disease Risk Factors","volume":"21","author":"Yang","year":"2017","journal-title":"Ther. Apher. Dial."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1016\/j.dsx.2019.07.048","article-title":"Urinary podocyte-associated mRNA profile in Egyptian patients with diabetic nephropathy","volume":"13","author":"Fayed","year":"2019","journal-title":"Diabetes Metab. Syndr."},{"key":"ref_28","first-page":"2358","article-title":"Messenger RNA expression of podocyte-associated molecules in urinary sediment of patients with lupus nephritis","volume":"34","author":"Wang","year":"2007","journal-title":"J. Rheumatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1053\/j.ajkd.2006.07.025","article-title":"Podocyte lesions in patients with obesity-related glomerulopathy","volume":"48","author":"Chen","year":"2006","journal-title":"Am. J. Kidney Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1002\/oby.21156","article-title":"Increased urine podocyte-associated messenger RNAs in severe obesity are evidence of podocyte injury","volume":"23","author":"Pereira","year":"2015","journal-title":"Obesity"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"320.e1","DOI":"10.1016\/j.ajog.2007.02.007","article-title":"Urinary podocyte excretion as a marker for preeclampsia","volume":"196","author":"Garovic","year":"2007","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1681\/ASN.2007121328","article-title":"Urine podocyte mRNAs mark progression of renal disease","volume":"20","author":"Sato","year":"2009","journal-title":"J. Am. Soc. 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